• Archives of Reconstructive Microsurgery
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• 제18권1호
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• pp.9-15
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• 2009

Whether a seven days course of anti-${\alpha}{\beta}$-T cell receptor-antibody (${\alpha}{\beta}$-TCRmAb) combined with FK506 therapy promotes survival of limb allografts in fully MHC-mismatched combination (Brown Norway $\rightarrow$ Lewis) was examined. Eight animals received 250 ${\mu}g$/kg/day of ${\alpha}{\beta}$-TCRmAb for 7 days and 2 mg/kg/day of FK506 postoperatively (Combination therapy group). Eight animals had FK506 only (Mono-therapy group) and five animals did not have treatment (Control group). Clinical signs of early rejection with edema or erythema in the skin occurred at an average of 8.6${\pm}$1.5 days postoperatively in Control group and 59.0${\pm}$8.3 days in Mono-therapy group, both of which proceeded to irreversible rejection with necrosis of the epidermis and finally mummification. In Combination therapy group, all animals showed evidence of early rejection at an average of 56.8${\pm}$12.6 days postoperatively, however, in 4 of 8 limbs, early rejection resolved without any treatment and limbs survived >1 year. At 9 months postoperatively, donor skin grafts were accepted and third-party skin grafts were rejected by all four survivors, demonstrating donor-specific tolerance. Little or no detectable chimerism was observed in any of the 4 surviving animals at one-year postoperatively. Combination therapy of ${\alpha}{\beta}$-TCRmAb and FK506 resulted in long-term survival in fully MHC-mismatched limb transplants.

• 농업과학연구
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• 제13권2호
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• pp.176-184
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• 1986

복숭아 혹 진딧물의 생물적(生物的) 방제(防除)를 위한 기초자료(基礎資料)를 얻기 위하여 85년 4~10월(月)까지 대전근교(大田近郊)의 포장에서 복숭아혹진딧물에 기생(寄生)하는 진디벌의 종류(種類)와 이에 기생하는 중기생벌을 조사하였고 진디벌과 중기생벌과의 관계, 주요 진디벌과 중기생벌의 mummy 형성 및 우화 등을 조사하였으며 진디벌의 생존기간(生存期間) 등을 조사(調査)한 결과(結果)는 다음과 같다. 1. 야외에서 채집(採集)된 201개(個)의 mummy 중 진디벌이 38.3% 중기생벌이 44.3%가 우화(羽化)되었다. 2. 복숭아 혹 진딧물에 기생하는 진디벌은 Lysiphlebia japonica와 Trioxys hokkaidenis가 가장 많았다. 3. 중기생벌은 Prataphelinus nikalskajae, Eucoila sp. Ardilea convexa가 많았다. 4. L. japonica가 T. hokkaidensis보다 중기생벌에게 더 많이 기생 당하고 있었다. 5. 종합적인 기생활동능력(寄生活動能力)은 T. hokkaidensis가 큰 것으로 나타났다. 6. L. japonica의 수명은 최대 3일이며 Glucose를 먹이로 하였을 때 가장 길었다.

• The Plant Pathology Journal
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• 제39권3호
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• pp.275-289
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• 2023

Fungal isolates from infected Chinese quince trees were found to cause black rot in Yeongcheon, Gyeongsangbuk Province, Korea. The quince leaves withered and turned reddish-brown and fruits underwent black mummification. To elucidate the cause of these symptoms, the pathogen was isolated from infected leaf and fruit tissues on potato dextrose agar and Levan media. Several fungal colonies forming a fluffy white or dark gray mycelium and two types of fungi forming an aerial white mycelium, growing widely at the edges, were isolated. Microscopic observations, investigation of fungal growth characteristics on various media, and molecular identification using an internal transcribed spacer, β-tubulin, and translation elongation factor 1-α genes were performed. The fungal pathogens were identified as Diplodia parva and Diplodia crataegicola. Pathogenicity tests revealed that the pathogen-inoculated fruits exhibited a layered pattern, turning brown rotting; leaves showed circular brown necrotic lesions. The developed symptoms were similar to those observed in the field. Fungal pathogens were reisolated to fulfill Koch's postulates. Apples were inoculated with fungal pathogens to investigate the host range. Strong pathogenicity was evident in the fruits, with browning and rotting symptoms 3 days after inoculation. To determine pathogen control, a fungicidal sensitivity test was conducted using four registered fungicides. Thiophanate-methyl, propineb, and tebuconazole inhibited the mycelial growth of pathogens. To the best of our knowledge, this is the first report on the isolation and identification of the fungal pathogens D. parva and D. crataegicola from infected fruits and leaves of Chinese quince, causing black rot disease in Korea.

• Parasites, Hosts and Diseases
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• 제27권2호
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• pp.119-130
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• 1989

흰쥐에 이락촌폐흡충(Paragonimus iloktsnenensis)을 감염시키고 폐흡충중의 특성과 진행 과정을 조사한 다음 praziquantel 투여가 폐 병변에 미치는 대과를 관찰하였다. 이락촌폐흡충 피낭 유충을 흰쥐에 감염시킨 바 전례에서 폐장에 병변을 유발하였고 육안적으로 폐 표면으로 돌출하는 출혈성 및 결절성 충낭들이 관찰되었으며, 현미경적으로 충낭과 아울러 폐실질 및 폐포 내에 충란에 의한 육아종 형성, 국소적 무기폐 및 염증 반응을 나타내었고, 병변 주위에는 육아조직과 결체조직의 증가가 동반되었다. 이락촌폐흡충 감염 5주 후에 prasiquantel을 투여한 바, 3일 후부터 이미 충체가 손상을 받아 지행성 변화를 보이기 시작하였고, 그 후로 충체가 점차 사멸, 소실되어 21일 후에 는 충체를 찾아볼 수 없었다 일부 흰쥐에서는 투약 후 14일까지 충체가 살아있는 경우도 관찰되었다. 대부분의 흰쥐에서는 충체가 사멸됨에 따라 폐장의 병변도 회복되기 시작하였는데 충남의 크기가 줄어들고, 충체가 흡수되며, 주위의 염증 반응과 무기폐 현상도 감소되었고, 산란양이 적어 충란 육아종 형성도 감염 대조군에 비해 경미하였다. 이상의 결과로 볼 때 흰쥐에 이락촌폐흡충을 감염시키면 인체의 폐흡충증과 거의 동일한 병변을 일으키며, praziquantel을 투여하면 1차적으로 충체가 손상, 사멸되면서 2차적으로는 폐 병변이 빠른 시간에 잘 복구되므로 결국 폐흡충증을 치유시키는데에 탁월한 효과가 있음을 알 수 있었다.

• 대한수의학회지
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• 제35권3호
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• pp.603-613
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• 1995

Plasma progesterone($P_4$) assay has been introduced to apply to the differential diagnosis of reproductive disorders and the monitoring of responses of ovarian dysfunction to $PGF_2{\alpha}$ or GnRH treatment in the 204 postpartum and postinsemination subestrus dairy cows. 1. The incidence rate of reproductive disorders in 204 subestrus cows, diagnosed by palpation per rectum and plasma $P_4$ determination using 'Two sample test'(Day 0+Day 10) were as follows; silent heat or error of estrus detection 110(53.9%), persistent corpus luteum 26(12.7%), follicular cyst 16(7.8%), inactive ovary 12(5.9%), luteal cyst 11(5.4%), granulosa cell tumor of ovary 1(0.5%), fetal mummification 1(0.5%), endometritis 15(7.4%) and pyometra 12(5.9%), respectively. 2. After the $PGF_2{\alpha}$ treatment to the 76 cows with silent heat or error of estrus detection, persistent corpus luteum, or luteal cyst, plasma $P_4$ concentrations at day 3 post treatment using 'Two sample test'(Day 0+Day 3) remained low(<1.0ng/ml) in all 76 cows. Therefore all 76 cows responded positively to $PGF_2{\alpha}$ treatment. Seventeen cows with follicular cyst or inactive ovary were treated with GnRH. All 7 cows with follicular cyst and 4 cows with inactive ovary remained high($${\geq_-}1.0ng/ml$$) a plasma $P_4$ concentrations at day 12 post treatment using 'Two sample test'(Day 0+Day 12), but 6 cows with inactive ovary remained low(<1.0ng/ml) a plasma $P_4$ concentrations. Therefore all 7 cows with follicular cyst and 4 cows with inactive ovary responded positively, but 6 cows with inactive ovary responded negatively to GnRH treatment. 3. The mean days from treatment to first service, number of cows conceived on first service(%), mean number of services per conception, mean days from initial treatment to conception, and mean number of cows conceived by 100 days post treatment(%) were 5.0 and 26.2 days, 45(59.2%) and 6(35.3%) cows, 1.5 and 1.7 services, 13.6 and 22.6 days, and 62(81.6%) and 9(52.9%) cows in group of $PGF_2{\alpha}$ and GnRH treatment, respectively. These results indicated that plasma $P_4$ assay was practical as an aid to diagnosing reproductive disorders and to monitoring responses of ovarian dysfunction to $PGF_2{\alpha}$ and GnRH treatment in subestrus cows.