• IMMUNE NETWORK
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• v.20 no.5
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• pp.40.1-40.15
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• 2020

The protein encoded by the Gene Associated with Retinoid-Interferon-Induced Mortality-19 (GRIM-19) is located in the mitochondrial inner membrane and is homologous to the NADH dehydrogenase 1-alpha subcomplex subunit 13 of the electron transport chain. Multiple sclerosis (MS) is a demyelinating disease that damages the brain and spinal cord. Although both the cause and mechanism of MS progression remain unclear, it is accepted that an immune disorder is involved. We explored whether GRIM-19 ameliorated MS by increasing the levels of inflammatory cytokines and immune cells; we used a mouse model of experimental autoimmune encephalomyelitis (EAE) to this end. Six-to-eight-week-old male C57BL/6, IFNγ-knockout (KO), and GRIM-19 transgenic mice were used; EAE was induced in all strains. A GRIM-19 overexpression vector (GRIM19 OVN) was electrophoretically injected intravenously. The levels of Th1 and Th17 cells were measured via flow cytometry, immunofluorescence, and immunohistochemical analysis. IL-17A and IFNγ expression levels were assessed via ELISA and quantitative PCR. IL-17A expression decreased and IFNγ expression increased in EAE mice that received injections of the GRIM-19 OVN. GRIM19 transgenic mice expressed more IFNγ than did wild-type mice; this inhibited EAE development. However, the effect of GRIM-19 overexpression on the EAE of IFNγ-KO mice did not differ from that of the empty vector. GRIM-19 expression was therapeutic for EAE mice, elevating the IFNγ level. GRIM-19 regulated the Th17/Treg cell balance.

• Horticultural Science & Technology
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• v.28 no.3
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• pp.442-448
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• 2010

The objectives of this study were to analyze mutant lines of Chinese cabbage ($Brassica$ $rapa$ ssp. $pekinensis$) using gene tagging system (plasmid rescue and inverse polymerase chain reaction) and to observe the phenotypic characteristics. Insertional mutants were derived by transferring DNA (T-DNA) of $Agrobacterium$ for functional genomics study in Chinese cabbage. The hypocotyls of Chinese cabbage 'Seoul' were used to obtain transgenic plants with $Agrobacterium$ $tumefaciens$ harboring pRCV2 vector. To tag T-DNA from the Chinese cabbage genomic DNA, plasmid rescue and inverse PCR were applied for multiple copies and single copy insertional mutants. These techniques were successfully conducted to Chinese cabbage plant with high efficiency, and as a result, T-DNA of pRCV2 vector showed distinct various integration patterns in the transgenic plant genome. The polyploidy level analysis showed the change in phenotypic characteristics of 13 mutant lines was not due to variation in somatic chromosome number. Compared with wild type, the $T_1$ progenies showed varied phenotypes, such as decreased stamen numbers, larger or smaller flowers, upright growth habit, hairless leaves, chlorosis symptoms, narrow leaves, and deeply serrated leaves. The polyploidy level analysis showed the change in phenotypic characteristics of 13 mutant lines was not due to variation in somatic chromosome number. To tag T-DNA from the Chinese cabbage genomic DNA, plasmid rescue and inverse PCR were applied for multiple copies and single copy insertional mutants. Mutants that showed distinct phenotypic difference compared to wild type with 1 copy of T-DNA by Southern blot analysis, and with 2n = 20 of chromosome number were selected. These selected mutant lines were sequenced flanking DNA, mapped genomic loci, and the genome information of the lines is being recorded in specially developed database.

• Tuberculosis and Respiratory Diseases
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• v.39 no.6
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• pp.517-525
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• 1992

Background: Since its development by Saiki et al, polymerase chain reaction (PCR) has been very useful in various fields of molecular biology. PCR can be used for the detection of a very small amount of microbial agent, and is especially useful in those patients who are difficult to diagnose microbiologically or serologically. Mycobacterium tuberculosis is a very slowly growing organism and AFB staining frequently shows false negative results, and therefore PCR would be a very rapid, easy, and sensitive diagnostic method for the diagnosis of Mycobacterium tuberculosis. Method: To compare PCR with conventional methods in diagnosing Mycobacterium tuberculosis in sputum, we used sputa of patients who visited or were admitted to Seoul National University Hospital. The amplification targets were 383 base pair DNA, a part of 2520 base pair DNA encoding 65 kD Mycobacterium tuberculosis specific protein (the primers are TB-1, -2), and 123 base pair DNA, a part of IS6110 fragment, which multiple copies are known to exsist PCR one genome (the primers are Sal I-1, -2). We also requested AFB staing and culture to the lab of Seoul National University Hospital with the same sample and compared the results. Results: 1) Using TB-1, -2 primers, PCR was positive in 73.1% (19/26) of culture positive sputa, in 12.5% (1/8) of culture negative. but clinically diagnosed tuberculous sputa, and was negative in all sputa of patients who were clinically diagnosed as non-tuberculous etiology. 2) Using Sal I-I, -2 primers, PCR was positive in 94.1% (32/34) of culture positive sputa, in 23.1% (6/26) of culture negative, but clinically diagnosed tuberculous sputa, and was negative in 87.5% (14/16) of sputa from patients who were clinically diagnosed as non-tuberculous etiology. Conclusion: PCR could be a very rapid, sensitive and specific method for the diagnosis of Mycobacterium tuberculosis in sputa, and further studies should be followed for the development of easier method.

• Spatial Information Research
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• v.4 no.2
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• pp.131-146
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• 1996

The purpose of this study is to extract the main locational factors to affect the location of fastfood stores in Kangnam -Gu, Seoul by using Geographic Information Systems. The Franchise system, which came to be employed in full scale since 1990, now enjoying the booming period, especially fast food industry. The procedure of this research has four steps. First, the spatial distribution of fastfood chain stores in Seoul is analyzed by the land use and road map. Second, the spatial variations of fastfood stores in dong districts of Seoul is explained by multiple regression analysis. Third, the main locational factors to affect the location of fast food stores in Kangnam -Gu are extracted by demand and supply sides using GIS technologies. Finally, the potentiallocational zones where are selected by extracted locational factors are compared with the actual distribution of f astf ood stores in Kangnam - Gu. In terms of demand side, the main locational factors include commercial and service facilities, sub¬ways and bus stops which have a lot of pedestrians, and large apartment districts that have high popu¬lation densities. In terms of supply sides, the main locational factors include land use types and land value, accessibility. After fast food chain stores of Kangnam -Gu are overlaid final potentiallocational zones extracted by demand and supply sides of locational factors, it can be identified that over 80 % of fastfood stores are located in the potentiallocational zone. In conclusion, this study shows that spatial analysis functions may potentially be improved using GIS technologies. However there are still difficulties for the locational analysis for service industries to col¬lect appropriate data in terms of computerized base maps as well as consumer behavior and store characteristics itself.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.10
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• pp.178-184
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• 2017

This study was performed on 16 patients diagnosed with tsutsugamushi disease and cerebral infarction from January 2007 to December 2015. An acute cerebral infarction was diagnosed by brain MRI and MRA. Tsutsugamushi disease was diagnosed using a polymerase chain reaction. To distinguish the difference between the generalized cerebral infarction and infarction with tsutsugamushi disease, the blood pressure and body temperature were measured uponadmission. In general, the blood pressure increases during an acute cerebral infarction. Interestingly, in this study, 12 patients showed a systolic blood pressure less than 130 mmHg uponadmission. The location of the cerebral infarction and whether single or multiple cerebral infarction were examined. Thirteen patients had a cerebral infarction in anterior circulation and 3 patients developed in posterior circulation. To evaluate the coagulation disorders, prothrombin time (PT), activated partial thromboplastin time (aPTT), D-dimer, fibrinogen, fibrin degradation product (FDP). D-dimer, which is generally known to increase in an acute cerebral infarction, showed a significant increase in the 13 patients. Fibrin degradation products (FDP) showed a significant increase in 15 patients. The pathophysiological mechanism of tsutsugamushi disease is known as vasculitis, which may result in an endothelial cell injury and proliferation of the endothelial wall, which may lead to a cerebral infarction accompanied by coagulopathy. Without endothelial cell damage and proliferation, a vasospasm caused by vasculitis may cause vasoconstriction and cerebral infarction.

• Molecules and Cells
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• v.27 no.2
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• pp.175-181
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• 2009

The myosin light chain kinase (MYLK) gene encodes both smooth muscle and nonmuscle cell isoforms. Recently, polymorphisms in MYLK have been reported to be associated with several diseases. To examine the genetic effects of polymorphisms on the risk of asthma and related phenotypes, we scrutinized MYLK by re-sequencing/genotyping and statistical analysis in Korean population (n = 1,015). Seventeen common polymorphisms located in or near exons, having pairwise $r^2$ values less than 0.25, were genotyped. Our statistical analysis did not replicate the associations with the risk of asthma and log-transformed total IgE levels observed among African descendant populations. However, two SNPs in intron 16 (+89872C> G and +92263T> C), which were in tight LD (|D'| = 0.99), revealed significant association with log-transformed blood eosinophil level even after correction multiple testing ($P=0.002/P^{corr}=0.01$ and $P=0.002/P^{corr}=0.01$, respectively). The log-transformed blood eosinophil levels were higher in individuals bearing the minor alleles for +89872C> G and +92263T> C than in those bearing other allele. In additional subgroup analysis, the genetic effects of both SNPs were much more apparent among asthmatic patients and atopic asthma patients. Among atopic asthma patients, the log-transformed blood eosinophil levels were proportionally increased by gene-dose dependent manner of in both +89872C> G and +92263T> C(P = 0.0002 and P = 0.00007, respectively). These findings suggest that MYLK polymorphisms might be among the genetic factors underlying differential increases of blood eosinophil levels among asthmatic patients. Further biological and/or functional studies are needed to confirm our results.

• Journal of Korea Water Resources Association
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• v.52 no.8
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• pp.545-554
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• 2019

The most drought assessments are based on a drought index, which depends on univariate variables such as precipitation and soil moisture. However, there is a limitation in representing the drought conditions with single variables due to their complexity. It has been acknowledged that a multivariate drought index can more effectively describe the complex drought state. In this context, this study propose a Copula-based drought index that can jointly consider precipitation and soil moisture. Unlike precipitation data, long-term soil moisture data is not readily available so that this study utilized a Gaussian Mixture Non-Homogeneous Hidden Markov chain Model (GM-NHMM) model to simulate the soil moisture using the observed precipitation and temperature ranging from 1973 to 2014. The GM-NHMM model showed a better performance in terms of reproducing key statistics of soil moisture, compared to a multiple regression model. Finally, a bivariate frequency analysis was performed for the drought duration and severity, and it was confirmed that the recent droughts over Jeollabuk-do in 2015 have a 20-year return period.

• Journal of the Korean Data Analysis Society
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• v.20 no.6
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• pp.2967-2980
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• 2018

In this study, we intended to find out how to improve the relationship performance between a hospital who is a buyer and its suppliers in the medical supply chain. For specific, in control of the transaction period and the number of bed, we investigated the effect of information sharing between a buyer and its suppliers along with the trust for the suppliers' capabilities perceived by the buyer on the buyer's relationship performance. In addition, in control of the transaction period and the number of bed, we also examined the influences of moderating effect of the interactional justice on the relations between information sharing and relationship performance, and between capability-trust and relationship performance. For this, we conducted reliability analysis, exploratory/confirmatory factor analyses, discriminant validity analysis, and moderated multiple regression analysis including control variables. Our results showed that there are positive effects of information sharing between a buyer and suppliers, and the buyer's perceived trust for the suppliers' capability on the relationship performance while controlling the transaction period and the number of bed. Besides, we empirically confirmed that there was the moderating effect of the interactional justice on the relations between capability-trust and relationship performance, whereas we could not find that statistically significant moderating effect of the interactional justice on the relations between information sharing and relationship performance.

• Journal of the Daesoon Academy of Sciences
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• v.25_2
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• pp.177-213
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• 2015

We live in two giant pendulum in called 'science' and 'religion'. But science and religion are contained in disparate information with each other, Those two is not easy to achieve convergence. But if you accept the ontological scheme of Great Chain of Being(存在의 大連鎖) in the Perennial philosophy(永遠의 哲學), Debate between religion and science is meaningless 'Great Nest of Being(存在의 대둥지, Great Chain of Being)' is similar to the multiple concentric circles, there are different dimension that the each top level to subsume surrounding the lower level. For example, upper zone 'Mysticism(神秘主義)' includes but transcends(or transcends but includes.) the sub-region theology, psychology, biology and physics. The Perennial philosophy is the great spiritual teachers of the world, philosophers and thinkers have adopted a common worldview, a religious views. Philosophers of the perennial philosophy seem to match the cross-cultural almost unanimous about the general level of the 'Great Nest of Being' for the past 3,000 years. The perennial philosophy made the conclusion that God exists in the world. Several types of view of God existing religions in the world have 'Monotheism(一神論)', 'Pantheism(汎神論)' and 'Panentheism (汎在神論)'. Although traditionally the God of the philosophers is the classical Theism, theological trends of today it is moving in the direction of Panentheism. Panentheism see that god is immanent and transcendent. also Daesoon Thoughts is the position of the Panentheism. so this paper points out the fact that the view of God of the perennial philosophy is precisely consistent with the view of God of Daesoon Thoughts. Wilber says 'envelopment [transcend and include]'. The word translates as 'powol(包越)' in Korean. 'Powol(包越)' means that all the developmental evolution is to surround the sub-region developed into the higher realms. View of the God in the perennial philosophy is 'powol theism(包越的 有神論)'. but 'powol immanent God(包越的 內在神論)' rather than building regulations as 'powol theism(包越的 有神論)'. It would have to be a more accurate representation of it. Because in the existing 'theism(有神論)' the god and humans are thought to exist apart. However, Daesoon Thoughts are deemed to also recognize another universal laws. also Sangje(上帝, the Supreme God) is recognized as a cosmic existence that transcends the laws. This point, as the characteristics of the Daesoon Thoughts, In other religions can not be found. Therefore, More specifically represent(More accurately represent), Sangje of the Daesoon Thoughts can be described as 'powol theism' or 'transcendental and included deism(包越的 理神論)'. Importantly, The idea of God can be captured directly by the discipline. In this sense, In terms of the other religions have no discipline law, the practice [discipline] of the Daesoon Thoughts required in the present age. It has the absoluteness.

• Journal of Korean Society of Archives and Records Management
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• v.24 no.2
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• pp.89-112
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• 2024

Metadata is a crucial component of record management, playing a vital role in properly managing and understanding the record. In cases where automatic metadata assignment is not feasible, manual input by records professionals becomes necessary. This study aims to alleviate the challenges associated with manual entry by proposing a method that harnesses ChatGPT technology for extracting records management metadata elements. To employ ChatGPT technology, a Python program utilizing the LangChain library was developed. This program was designed to analyze PDF documents and extract metadata from records through questions, both with a locally installed instance of ChatGPT and the ChatGPT online service. Multiple PDF documents were subjected to this process to test the effectiveness of metadata extraction. The results revealed that while using LangChain with ChatGPT-3.5 turbo provided a secure environment, it exhibited some limitations in accurately retrieving metadata elements. Conversely, the ChatGPT-4 online service yielded relatively accurate results despite being unable to handle sensitive documents for security reasons. This exploration underscores the potential of utilizing ChatGPT technology to extract metadata in records management. With advancements in ChatGPT-related technologies, safer and more accurate results are expected to be achieved. Leveraging these advantages can significantly enhance the efficiency and productivity of tasks associated with managing records and metadata in archives.