• Asian Pacific Journal of Cancer Prevention
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• 제17권1호
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• pp.255-259
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• 2016

Background: Reliable and validated instruments are needed in order to study the quality of life in myeloma patients. This study aimed to translate and explore the psychometric properties of the European Organisation for Research and Treatment of Cancer (EORTC) myeloma module (QLQ-MY20) in Iranian patients. Materials and Methods: Two hundred and fifteen patients with multiple myeloma (MM) were recruited from Imam Khomeini Hospital, Tehran. A standard forward-backward translation procedure was implemented. Participating patients were asked to complete the EORTC QLQ-C30 and the QLQ-MY20 three times, at study entry, after two weeks, and again after three months. Data were tested for the range of measurement, internal consistency, test-retest reliability, known group comparison, responsiveness and factor structure. Results: Mean age of the patients was 60.7 years. No floor and ceiling effects were seen for the QLQ-MY20. Cronbach's ${\alpha}$ was greater than 0.80 for all three multi-item scales (ranging from 0.82 to 0.93). All four scales had test-retest reliability of 0.85 or greater. Results of the confirmatory factor analysis that the hypothesized 3-scale measurement model of the QLQ-MY20. Moreover, the Persian version for the QLQ-MY20 differentiated between subgroups of the patients in terms of beta-2 microglobulin, fracture and performance status. The responsiveness of the QLQ-MY20 to change over time was confirmed within 3 months. Conclusions: the results of our study indicate that our Iranian version of the QLQ-MY20 is a feasible, reliable and valid questionnaire for assessing the condition-specific quality of life of patients with MM.

• Journal of Photoscience
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• 제9권2호
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• pp.182-185
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• 2002

There are 3 UV DNA repair endonuclease activities in mammalian cells that cleave UV -irradiated DNA. Interestingly, mammalian UV endonuclease III with MW of 26.7kD has a lyase activity on AP sites. It also cleaves the phosphodiester bond within a cyclobutane pyrimidine dimer. Genomic analysis of human repair endonuclease III gene revealed that this gene has 100% sequence identity with ribosomal protein S3 (rpS3). Therefore, rpS3 seems to function both in translation and in DNA repair. This gene of about 6.1 kb contains 6 introns and 7 exons, and the first and fifth introns of human rpS3 gene contain functional U15 small nucleolar (sno) RNAs which appear to be involved in ribosome assembly. It is to be noted that the column profile of the endonuclease activity of rpS3 appears to be altered in Xeroderma Pigmentosum (XP) group D cells compared to normal cells indicating that this protein is involved in XP disease as well. XP is a human disease characterized by high sensitivity of skin by UV- or sun-light irradiation and by high frequency of developing skin cancers. We also report here that rpS3 protein is involved in other cellular functions.

• 영어어문교육
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• 제1호
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• pp.69-108
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• 1995

Listening comprehension can be defined as a process of an integrative, positive and creative activity through which listeners get the message of speakers' production using linguistic or non-linguistic redundancy as well as linguistic or non-linguistic knowledge. Compared with reading comprehension, it has many difficulties especially for foreigners. while it can be transferred to the other skills: speaking, reading, writing. With this said, listening comprehension can be taught effectively using the following teaching strategies. First. systematic and intensive instruction of segmental phonemes, suprasegmental phonemes and sound changes must be given to remove the difficulties of listening comprehension concerned with the identification of sounds. Second, vocabulary drill through various games and other activities is absolutely needed until words can be unconsciously recognized. Without this, comprehension is almost impossible. Third, instruction of sentence structures is thought to be essential considering grammar is supplementary to listening comprehension and reading comprehension for academic purpose. So grammar translation drills, mechanical drills, meaningful drills and communicative drills should be performed in succession with common or frequently used structures. Fourth, listening activities for overall comprehension should teach how to receive overall meaning of intended messages intact. Linguists and literatures have listed some specific activities as follows: Total Physical Response, dictation, role playing, singing songs, selective listening, picture recognition, list activities, completion, prediction, true or false choice, multiple choice, seeking of specific information, summarizing, problem-solving and decision-making, recognization of relationships between speakers, recognition of mood, attitude and behavior of speakers.

• Mycobiology
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• 제45권1호
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• pp.39-43
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• 2017

Leaf spot disease on black chokeberry (Aronia melanocarpa) was observed at several locations in Korea during 2014-2015. Leaf spots were distinct, scattered over the leaf surface and along the leaf border, subcircular to irregular and brown surrounded by a distinct dark color, and were expanded and coalesced into irregularly shaped lesions. Severely infected leaves became dry and fell off eventually. The causative agent was identified as Pseudocercospora pyricola. Morphological observations and phylogenetic analyses of multiple genes, including internal transcribed spacer, translation elongation factor 1-alpha, actin, and the large subunit ribosomal DNA were conducted. The pathogenicity test was conducted twice yielding similar results, fulfilling Koch's postulates. To our knowledge, this is the first report on P. pyricola infection of A. melanocarpa globally.

• Genomics & Informatics
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• 제15권4호
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• pp.136-141
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• 2017

Accurate detection of genomic alterations, especially druggable hotspot mutations in tumors, has become an essential part of precision medicine. With targeted sequencing, we can obtain deeper coverage of reads and handle data more easily with a relatively lower cost and less time than whole-exome or whole-genome sequencing. Recently, we designed a customized gene panel for targeted sequencing of major solid cancers. In this study, we aimed to validate its performance. The cancer panel targets 95 cancer-related genes. In terms of the limit of detection, more than 86% of target mutations with a mutant allele frequency (MAF) <1% can be identified, and any mutation with >3% MAF can be detected. When we applied this system for the analysis of Acrometrix Oncology Hotspot Control DNA, which contains more than 500 COSMIC mutations across 53 genes, 99% of the expected mutations were robustly detected. We also confirmed the high reproducibility of the detection of mutations in multiple independent analyses. When we explored copy number alterations (CNAs), the expected CNAs were successfully detected, and this result was confirmed by target-specific genomic quantitative polymerase chain reaction. Taken together, these results support the reliability and accuracy of our cancer panel in detecting mutations. This panel could be useful for key mutation profiling research in solid tumors and clinical translation.

• Structural Engineering and Mechanics
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• 제57권6호
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• pp.989-1014
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• 2016

This paper deals with the analysis of the natural frequencies, mode shapes of an axially loaded beam system carrying ends consisting of non-concentrated tip masses and three spring-two mass sub-systems. The influence of system design and sub-system parameters on the combined system characteristics is the major part of this investigation. The effect of material properties, rotary inertia and shear deformation of the beam system is included. The end masses are elastically supported against rotation and translation at an offset point from the point of attachment. Sub-systems are attached to center of gravity eccentric points out of the beam span. The boundary conditions of the ordinary differential equation governing the lateral deflections and slope due to bending of the beam system including developed shear force frequency dependent terms, due to the sub.system suspension, have been formulated. Exact formulae for the modal frequencies and the modal shapes have been derived. Based on these formulae, detailed parametric studies are carried out. The geometrical and mechanical parameters of the system under study have been presented in non-dimensional analysis. The applied mathematical model is presented to cover wide range of mechanical, naval and structural engineering applications.

• 생물정신의학
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• 제23권4호
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• pp.173-178
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• 2016

Objectives The purpose of this study was to investigate the relationships among circadian types, depressive mood and internet addiction and to identify the mediating effect of depressive mood between circadian types and internet addiction in Korean College Students. Methods A total of 2632 participants completed questionnaires, which included the Korean translation of the Composite Scale of Morningness (CSM), the Patient Health Questionnaire (PHQ-9), and the Korean version of the Internet Addiction Test (KIAT). Statistical analyses were done using correlation analysis, analysis of covariance and multiple linear regression. Results The CSM score was negatively associated with the PHQ-9 score (r = -0.226, p < 0.001) and the KIAT score (r = -0.218, p < 0.001). The eveningness group showed higher scores in depressive mood and internet addiction than the morningness group (p < 0.001). Depressive mood showed a partial mediating effect between circadian types and internet addiction (${\beta}=0.255$, p < 0.001). Conclusions Internet addiction was associated with circadian types and depressive mood. These results suggest that depression and circadian rhythm management would contribute to the prevention of internet addiction.

• 대한한의학원전학회지
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• 제28권1호
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• pp.97-110
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• 2015

Objectives : Usually medical terminology of oriental mecidine has a multiple meaning. But concept of Shanghan(傷寒) should be simple, because Shanghanlun(傷寒論) is a clinical guideline book. So I researched to suggest many concept of Shanghan, which are suitable for each chapter of Shanghanlun. Methods : I enumerated provisions including Shnaghan from the original texts of Gangpyeong-Shanghanlun(康平傷寒論). And I translated and reviewed them. Results : 1. Shanghan of Preface(序文) means a disease of high fatality. 2. Shanghan of Shanghanrye(傷寒例) means diseases due to physical damage of cold weather. 3. Shanghan of Diagnosis of Daeyang Disease(辨大陽病) - Neck stiffness(痙), Dampness(濕), Sun stroke(暍) means certain disease names accompanying fever, chill. 4. Shanghan used in Diagnosis of Diseases is a premise of many provisions of Shanghanlun. And Shanghan is made up of finished fever, expected fever, chill, body pain, loss of appetite, image of tension. Conclusions : We can use a appropriate translation on Shanghan of each chapter of Gangpyeong-Sanghanlun. Especially Shanghan used in "Diagnosis of Diseases" should have more accurate meaning.

• 대한물리의학회지
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• 제10권3호
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• pp.47-55
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• 2015

PURPOSE: Stoke is one of most common disabling conditions and it is still lacking of measuring patient's functioning level. The aim of the study was to develop Korean language version of stroke impact scale 3.0. METHODS: Korean version of stroke impact scale 3.0 was developed in idiomatic modern Korean with a standard protocol of multiple forward and backward translations and an expert reviews to achieve equivalence with the original English version. Interviews with clinicians who were currently managing patients with stroke were also conducted for language evaluation. A reliability test was performed to make final adaptation using a pre-final version. To assess the reliability of the translated questionnaire, the intraclass correlation coefficient (ICC) was calculated for each domain of the scale. RESULTS: Thirty subjects (16 male, 14 female) aged from 20 to 75 years old participated to review the translated questionnaire. Reliability of each domain of the questionnaire was found to be good in strength (ICC=0.74), ADL (ICC=0.81), mobility (ICC=0.90), hand function (ICC=0.80) and social participation (ICC=0.79), communication (ICC=0.77) with total (ICC=0.76). However, domains of memory and thinking (ICC=0.66), and emotion (ICC=0.27) and showed poor reliability. CONCLUSION: This study indicates that the Korean version of SIS 3.0 was successfully developed. Future study needed for obtaining the validity of the Korean version of SIS 3.0.

• Animal cells and systems
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• 제13권4호
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• pp.381-389
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• 2009

Using the DDRT-PCR, a series of differentially expressed genes in human primary cervical cancer was isolated. Among the 250 PCR amplimers, 88 gene fragments were confirmed by reverse Northern hybridization. Homology searches indicated that 26 out of 88 were previously known genes including calmodulin, human BBC1, histone H3.3, a series of ribosomal proteins (RPL19, RPS19, and RPS12), translation initiation factor (eIF-4AI), lactoferrin, integrin ${\alpha}6$, cell-surface antigens (CD9 and CD59), transcription factor (mbp-1), and mitochondrial proteins. Several unknown clones showed sequence homology with known genes. Furthermore, six of the unknown genes showed identical sequence with expressed sequence tags (EST) of unknown function. Differential expression patterns of identified genes were further examined and confirmed with multiple pairs of cervical cancer samples using Northern hybridization. Our profiling of differentially expressed genes may provide useful information about the underlying genetic alterations in human cervical carcinoma and diagnostic markers for this disease. The precise roles of these genes in cancer development remain to be elucidated.