• Journal of Photoscience
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• 제3권2호
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• pp.85-90
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• 1996

The binding ability of leaf nuclear extracts to the lighbresponsive elements (LREs) of cab promoters of Arabidopsis thaliana has been investigated. The cab promoters were fragmented with restr ction endonucleases into LRE that were identified by Mitra et al. [Plant Mol. Biol. 12, 169179 ( 1989)] and other small fragments. After end labeling with Klenow fragment, the fragments were assayed for binding with the leaf nuclear proteins that were prepared by solubilizing the purified nuclei with 0.5 M ammonium sulfate. The binding ability was assayed by mobility shift assay. To perform successful mobility shift assay, several factors affecting the interaction of protein with DNA were optimized before performing the assay. The LREs had several retardation bands. However, the other promoter fragments from the transcription start site to the far upstream region of the promoters had also retardation bands. No particular relationships could be found between the retardation band distributions and the loci of LRE. It is likely that the light-regulation of cab gene expression may be controlled by the multiple interactions of the regulatory protein factors with DNA motifs.

• 인지과학
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• 제10권1호
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• pp.25-43
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• 1999

본 연구는 사이버 공간에서 사용자와 시스템간의 상호작용을 도울 수 있는 구조디자인 설계에 초점을 맞추고 있다. 구조디자인이란 인터페이스를 구성하는 개별화면이 어떻게 분류되며 이에 따라서 화면내의 정보가 어떻게 표현되어야 하는지를 결정하는 것이다. 본 연구에서는 사이버 쇼핑몰 안에서 쇼핑만족과 항해 편리성을 높일 수 있는 구조디자인을 설계하기 위한 방안으로 교차적 연결과 다 계층 구조를 제시하였다. 교차적 연결이란 여러 가지 범주화 기준이 적용된 정보공간 사이를 연결시켜주고 것이고, 다 계층 구조란 동일한 정보공간에 대해서 여러 가지 기준을 가지고 각 기준에서 바라본 관점으로 정보공간을 표현해주는 구조이다. 본 연구에서는 교차적 연결과 다 계층 구조의 유용성 검증을 위한 두 단계의 실험을 사이버 쇼핑몰을 대상으로 실시하였다. 제1단계 실험은 사용자의 특정 정보 공간에 대한 인지적 틀, 즉 상품 정보 공간에 대한 사용자 관점에서의 다양한 범주화 기준을 파악함으로써 사이버 쇼핑몰의 기본 상품분류체계를 개발하였으며, 제2단계 실험은 1단계 실험결과를 바탕으로 교차적 연결과 다 계층 구조를 사용한 쇼핑몰 구조에 대한 유용성을 검증하는 것이었다. 그 검증 결과는 교차적 연결 측면에서는 교차적 연결이 제공되는 쇼핑몰이 제공되지 않는 쇼핑몰 보다 항해 편리성이 높은 것으로 나왔다. 그리고 이 교차적 연결이 다 층 구조상에서 제공되는 것보다는 단일 계층 구조상에서 제공되는 것이 항해 편리성을 높이고 있다. 한편, 계층구조측면에서는 단일 계층 구조가 제공되는 쇼핑몰이 다 계층 구조가 제공되는 쇼핑몰보다 항해만족과 항해 편리성이 높은 것으로 나왔다. 이를 기초로 본 연구는 이러한 결과에 대한 토론 및 쇼핑몰 구축상의 시사점을 제시하였다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권6호
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• pp.3735-3742
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• 2013

Background: Cardamom (Elettaria cardamom), also known as "Queen of Spices", has been traditionally used as a culinary ingredient due to its pleasant aroma and taste. In addition to this role, studies on cardamom have demonstrated cancer chemopreventive potential in in vitro and in vivo systems. Nevertheless, the precise poly-pharmacological nature of naturally occurring chemo-preventive compounds in cardamom has still not been fully demystified. Methods:In this study, an effort has been made to identify the proapoptopic, anti-inflammatory, anti-proliferative, anti-invasive and anti-angiogenic targets of Cardamom's bioactive principles (eucalyptol, alpha-pinene, beta-pinene, d-limonene and geraniol) by employing a dual reverse virtual screening protocol. Experimentally proven target information of the bioactive principles was annotated from bioassay databases and compared with the virtually screened set of targets to evaluate the reliability of the computational identification. To study the molecular interaction pattern of the anti-tumor action, molecular docking simulation was performed with Auto Dock Pyrx. Interaction studies of binding pose of eucalyptol with Caspase 3 were conducted to obtain an insight into the interacting amino acids and their inter-molecular bondings. Results:A prioritized list of target proteins associated with multiple forms of cancer and ranked by their Fit Score (Pharm Mapper) and descending 3D score (Reverse Screen 3D) were obtained from the two independent inverse screening platforms. Molecular docking studies exploring the bioactive principle targeted action revealed that H- bonds and electrostatic interactions forms the chief contributing factor in inter-molecular interactions associated with anti-tumor activity. Eucalyptol binds to the Caspase 3 with a specific framework that is well-suited for nucleophilic attacks by polar residues inside the Caspase 3 catalytic site. Conclusion:This study revealed vital information about the poly-pharmacological anti-tumor mode-of-action of essential oils in cardamom. In addition, a probabilistic set of anti-tumor targets for cardamom was generated, which can be further confirmed by in vivo and in vitro experiments.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2009년도 제38회 동계학술대회 초록집
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• pp.13-13
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• 2010

Switching an elementary excitation by injecting a single carrier would offer the exciting opportunity for the ultra-high data storage technologies. However, there has been no methodology available to investigate the interaction of low energy discrete carriers with nano-structures. In order to map out the spatial dependency of such single carrier level interactions, we developed a pulse-and-probe algorithm, combining with low temperature scanning tunneling microscopy. The new tool, which we call single carrier spectroscopy, allows us to track the interaction with the target macrostructure with tunneling carriers on a single carrier basis. Using this tool, we demonstrate that it is possible not only to locally write and erase individual bi-solitons, reliably and reversibly, but also to track of creation yields of single and multiple bi-solitons. Bi-solitons are pairs of solitons that are elementary out-of-phase excitations on anti-ferromagnetically ordered pseudo-spin system of Si dimers on Si(001)-c(42) surfaces. We found that at low energy tunneling the single bisoliton creation mechanism is not correlated with the number of carriers tunneling, but with the production of a potential hole under the tip. An electric field at the surface determines the density of the local charge density under the tip, and band-bending. However a rapid, dynamic change of a field produces a potential hole that can be filled by energetic carriers, and the amount of energy released during filling process is responsible for the creation of bi-solitons. Our model based on the field-induced local hole gives excellent explanation for bi-soliton yield behaviors. Scanning tunneling spectroscopy data supports the existence of such a potential hole. The mechanism also explains the site-dependency of bi-soliton yields, which is highest at the trough, not on the dimer rows. Our study demonstrates that we can manipulate not just single atoms and molecules, but also single pseudo-spin excitations as well.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2003년도 춘계학술대회
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• pp.381-388
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• 2003

The material deficiencies in the form of pre-existing defects can initiated cracks and fractures. The stress distribution and fatigue crack initiation life of engineering materials may be associated with the size, the shape and the relative location of defects contained in the component. The objective of this study is to investigate the effect of arbitrarily located hole defect around the rivet hole of a wing section in monolithic aluminum and Al/GFRP laminates under cyclic bending moment during a service load. The stress distribution and the fatigue crack initiation behavior near a rivet hole of on the relationships between stress concentration factor ($K_t$) and relative position of defects were considered. The test results indicated the features of different stress field. Therefore, the stress concentration factor ($K_t$) and the fatigue crack initiation behavior was illustrated different behavior according to each position of hole defect around the rivet hole in monolithic aluminum and Al/GFRP laminates.

• Biomolecules & Therapeutics
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• 제20권3호
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• pp.346-351
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• 2012

Cells show various stress signs when they are challenged with severe physiological problems. Majority of such cellular stresses are conveyed to endoplasmic reticulum (ER) and unfolded protein response (UPR) serves as typical defense mechanism against ER stress. This study investigated an interaction between ER stress agents using macropage cell line Raw 264.7. When activated by lipopolysaccharide (LPS), the cell lines showed typical indicators of ER stress. Along with molecular chaperones, the activation process leads to the production of additional inflammatory mediators. Following activation, the macrophage cell line was further treated with TUN and characterized in terms of chaperone expression and cytokine secretion. When treated with TUN, the activated macrophage cell leads to increased secretion of IL-6 although expression of ER stress markers, GRP94 and GRP78 increased. The secretion of cytokines continued until the addition of BFA which inhibits protein targeting from ER to Golgi. However, secretion of cytokines was ceased upon dual treatments with BFA and TG. This result strongly implies that cells may differently deal with various polypeptides depending on the urgency in cellular function under ER stress. Considering IL-6 is one of the most important signal molecules in macrophage, the molecule might be able to circumvent ER stress and UPR to reach its targeting site.

• Nuclear Engineering and Technology
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• 제51권1호
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• pp.104-115
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• 2019

Since the Fukushima Daiichi accident, there has been an emphasis on the risk resulting from multi-unit accidents. Human reliability analysis (HRA) is one of the important issues in multi-unit probabilistic safety assessment (MUPSA). Hence, there is a need to properly identify all the human and organizational factors relevant to a multi-unit incident scenario in a nuclear power plant (NPP). This study identifies and categorizes the human and organizational factors relevant to a multi-unit incident scenario of NPPs based on a review of relevant literature. These factors are then analyzed to ascertain all possible unit-to-unit interactions that need to be considered in the multi-unit HRA and the pattern of interactions. The human and organizational factors are classified into five categories: organization, work device, task, performance shaping factors, and environmental factors. The identification and classification of these factors will significantly contribute to the development of adequate strategies and guidelines for managing multi-unit accidents. This study is a necessary initial step in developing an effective HRA method for multiple NPP units in a site.

• Bulletin of the Korean Chemical Society
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• 제26권2호
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• pp.285-291
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• 2005

The nonstructural protein 5B (NS5B) of hepatitis C virus (HCV) is the viral RNA-dependent RNA polymerase (RdRp), which is the essential catalytic enzyme for the viral replication and is an appealing target for the development of new therapeutic agents against HCV infection. A small amount of serum from a single patient with hepatitis C was used to get the genome of a Korean HCV isolate. Sequence analysis of NS5B 1701 nucleotides showed the genotype of a Korean isolate to be subtype 1b. The soluble recombinant HCV NS5B polymerase lacking the C-terminal 24 amino acids was expressed and purified to homogeneity. With the highly purified NS5B protein, we established in vitro systems for RdRp activity to identify potential polymerase inhibitors. The rhodanine family compounds were found to be potent and specific inhibitors of NS5B from high throughput screening (HTS) assay utilizing the scintillation proximity assay (SPA) system. The binding mode of an inhibitor was analyzed by measuring various kinetic parameters. Lineweaver-Burk plots of the inhibitor suggested it binds not to the active site of NS5B polymerase, but to an allosteric site of the enzyme. The activity of NS5B in in vitro polymerase reactions with homopolymeric RNA requires interaction with multiple substrates that include a template/primer and ribonucleotide triphosphate. Steady-state kinetic parameter, such as Km, was determined for the ribonucleotide triphosphate. One of compounds found interacts directly with the viral polymerase and inhibits RNA synthesis in a manner noncompetitively with respect to UTP. Furthermore, we also investigated the ability of the compound to inhibit NS5B-directed viral RNA replication using the Huh7 cell-based HCV replicon system. The investigation is potentially very useful for the utility of such compounds as anti-hepatitic agents.

• 한국지진공학회논문집
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• 제9권2호통권42호
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• pp.47-58
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• 2005

이산폰툰형 부유식교량의 시간영역 지진응답해석을 지진파의 공간분포를 고려하여 수행하였다. 지반운동의 공간변화는 파의 통과 영향, 엇결성 뎡향 및 국부지반 영향을 반영하는 결맞음 함수 모델로 고려하였다. 부유식 교량의 상부구조는 공간뼈대요소와 탄성현수케이블요소를 이용하여 모델링 하였고, FHWA 가이드라인의 스프링 모델을 사용하여 교대의 지반 구조물 상호작용에 대한 영향을 고려하엿으며, 경계요소법으로 산정한 폰툰 동수력계수의 주파수 의종성을 고려하기 위해서 시간지연함수를 사용하였다. 지반운동의 공간변화를 고려한 다중지점 지진입력을 교량의 양단에 도입하였고 응답의 시간이력을 동시 가진 시와 비교하였다. 장주기의 동적특성을 갖는 이산폰툰형 부유식 교량의 지진응답 해석에서 지반운동의 공간분포를 고려할 경우에 주몰할만한 응답의 증폭이 나타날 수 있음을 확인하였다.

• Archives of Plastic Surgery
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• 제51권1호
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• pp.94-101
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• 2024

Background For the small glabrous skin defect, Thenar and Hypothenar skin are useful donors and they have been used as a free flap. Because of similar skin characteristics, both flaps have same indications. We will conduct comparative study for the donor morbidity of the Free thenar flap and Hypothenar free flap. Methods From January 2011 to December 2021, demographic data, characteristics of each flap, and complications using retrospective chart review were obtained. Donor outcomes of the patient, who had been followed up for more than 6 months, were measured using photographic analysis and physical examination. General pain was assessed by Numeric Rating Scale (NRS) score, neuropathic pain was assessed by Douleur Neuropathique 4 Questions (DN4) score, scar appearance was assessed by modified Vancouver Scar Scale (mVSS), and patient satisfaction was assessed on a 3-point scale. Statistical analysis was performed on the outcomes. Results Out of the 39 survey respondents, 17 patients received Free thenar flaps, and 22 patients received Hypothenar free flaps. Thenar group had higher NRS, DN4, and mVSS (p < 0.05). The average scores for the Thenar and Hypothenar groups were 1.35 and 0.27 for NRS, 2.41 and 0.55 for DN4, and 3.12 and 1.59 for mVSS, respectively. Despite the Hypothenar group showing greater satisfaction on the 3-point scale (1.82) compared with the Thenar group (1.47), the difference was not significant (p = 0.085). Linear regression analysis indicated that flap width did not have a notable impact on the outcome measures, and multiple linear regression analysis revealed no significant interaction between flap width and each of the outcome measures. Conclusion Despite the limited number of participants, higher donor morbidity in general pain, neuropathic pain, and scar formation was noted in the Thenar free flap compared with the Hypothenar free flap. However, no difference in overall patient satisfaction was found between the two groups.