Journal of Photoscience

Korean Society of Photoscience (한국광과학회)
권호 표지

BINDING OF LEAF NUCLEAR PROTEIN EXTRACTS TO LIGHT-RESPONSIVIE ELEMENTS OF cab PROMOTERS OF Arabidopsis thaliana

  • Lee, Hwa-Hyung (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University) ;
  • Park, Hee-Jin (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University)
  • Published : 1996.06.01
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Abstract

The binding ability of leaf nuclear extracts to the lighbresponsive elements (LREs) of cab promoters of Arabidopsis thaliana has been investigated. The cab promoters were fragmented with restr ction endonucleases into LRE that were identified by Mitra et al. [Plant Mol. Biol. 12, 169179 ( 1989)] and other small fragments. After end labeling with Klenow fragment, the fragments were assayed for binding with the leaf nuclear proteins that were prepared by solubilizing the purified nuclei with 0.5 M ammonium sulfate. The binding ability was assayed by mobility shift assay. To perform successful mobility shift assay, several factors affecting the interaction of protein with DNA were optimized before performing the assay. The LREs had several retardation bands. However, the other promoter fragments from the transcription start site to the far upstream region of the promoters had also retardation bands. No particular relationships could be found between the retardation band distributions and the loci of LRE. It is likely that the light-regulation of cab gene expression may be controlled by the multiple interactions of the regulatory protein factors with DNA motifs.

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References

  1. Plant Cell v.1 Different roles for phytochrome in etiolated and green plants deduced from characterization of Arabidopsis thaliana mutants Chory, J.;C. A. Peto;M. Ashbaugh;R. Saganich;L. Pratt;F. Ausubel
  2. Plant Physiol. v.100 Phytochrome-mediated phototropism in de-etiolated seedlings Ballare, C. L.;A. L. Scopel;S. R. Radosevich;R. E. Kendrick
  3. Photomorphogenesis in Plants(Ed 2) Kendrick, R. E.;G. H. M. Kronenberg
  4. Ann. Rev. Plant Physiol. v.36 Light regulation of gene expression in higher plants Tobin, E. M.;J. Silverthorne
  5. EMBO J. v.5 Phytochrome-controlled expression of a wheat Cab gene in transgenic tobacco seedlings Nagy, F.;S. A. Kay;M. Boutry;M.-Y. Hsu;N.-H. Chua
  6. Plant Physiol. v.88 Expression of light-harvesting chlorophyll a/b-protein genes is phytochrome-regulated in etiolated Arabidopsis thaliana seedlings Karlin-Neumann, G. A.;L. Sun;E. M. Tobin
  7. Photochem. photobiol. v.52 Phytochrome-regulated expression of genes encoding light-harvesting chlorophyll a/b-binding in two long hypocotyl mutants and wild type plants of Arabidopsis thaliana Sun, L.;E. M. Tobin
  8. Plant Mol. Biol. v.19 Sequence of the fourth and fifth Photosystem Ⅱ Type Ⅰ chlorophyll a/b-binding protein genes of Arabidopsis thaliana and evidence for the presence of a full complement of the extended cab gene family McGrath, J. M.;W. B. Terzaghi;P. Sridhar;A. R. Cashmore;E. Pichersky
  9. Plant Mol. Biol. v.12 Structural and functional analyses of Arabidopsis thaliana chlorophyll a/b-binding protein (cab) promoters Mitra, A.;H. K. Choi;G. An
  10. Proc. Natl. Acad. Sci. USA v.85 Identification of upstream regulatory elements involved in the developmental expression of the Arabidopsis thaliana cab1 gene Ha, S. S.;G. An
  11. Mol. Gen. Genet. v.207 Integrated regulation of the photosynthetic gene family from Arabidopsis thaliana in transformed tobacco cells An, G.
  12. Nucleic Acids Res. v.14 Structure and expression of three light-harvesting chlorophyll a/b-binding protein genes in Arabidopsis thaliana Leutwiler, L. S.;E. M. Meyerowitz;E. M. Tobin
  13. Molecular Cloning: A Laboratory Mannual Maniatis, T.;E. F. Fritch;J. Sambrook
  14. Proc. Natl. Acad. Sci. USA v.85 An evolutionary conserved protein binding sequence upstream of a plant light-regulated gene Giuliano, G.;E. Pichersky;V. S. Malik;M. P. Timko;P. A. Scolnik;A. R. Cashmore
  15. Nature v.319 A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes Singh, H.;R. Sen;D. Baltimore;P. A. Sharp
  16. Plant Physiol. v.65 Transcription in isolated wheat nuclei Luthe, D. S.;R. S. Quatrano
  17. EMBO J. v.6 Sequence-specific interactions of a pea nuclear factor with light responsive elements upstream of the rbcS-3A gene Green, P. J.;S. A. Kay;N.-H. Chua
  18. Anal. Biochem. v.72 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Bradford, M. M.
  19. Electrophoresis v.10 Measurement of DNA-protein interaction parameters by electrophoresis mobility shift assay Fried, M. G.
  20. Plant Cell v.1 Binding of a pea nuclear proteins of certainphotoregulated genes is modulated by phosphorylation Datta, N.;A. R. Cashmore
  21. EMBO J. v.9 Photoregulated gene expression may involve ubiquitous DNA binding proteins Schindler, U.;A. R. Cashmore
  22. Plant Cell v.2 Molecular light switches for plant genes Gilmartin, P. M.;L. Sarokin;J. Memelink;N.-H. Chua
  23. Plant J. v.6 Circadian clock-and phytochrome-regulated transcription is conferred by a 78 bp cisacting domain of the Arabidopsis CAB2 promoter Anderson, S. L.;G. R. Teakle;S. J. Martino-Catt;S. A. Kay
  24. Proc. Natl. Acad. Sci. USA v.92 Functional dissection of circadian clock- and phytochrome- regulated transcription of the Arabidopsis CAB2 gene Anderson, S. L.;S. A. Kay
  25. Mol. Gen. Genet. v.215 Novel cis-acting elements in petunia cab gene promoters Gidoni, D.;P. Brosio;D. Bond-Nutter;J. Bedbrook;P. Dunsmuir
  26. EMBO J. v.7 Both positive and negative regulatory elements mediate expression of a photoregulated cab gene from Nicotiana plumbaginifolia Castresana, C.;I. Garcia-Luque;E. Alonso;V. S. Malk;A. R. Cashmore