• Mycobiology
• /
• v.47 no.4
• /
• pp.466-472
• /
• 2019

For the purpose of protecting the rights of Lentinula edodes breeders, we developed a new simple sequence repeat (SSR) marker set consisting only of genetically independent tetranucleotide or longer core motifs. Using available genome sequences for five L. edodes strains, we designed primers for 13 SSR markers that amplified polymorphic sequences in 20 L. edodes cultivars. We evaluated the independence of every possible marker pair based on genotype data. Consequently, eight genetically independent markers were selected. The polymorphic information content values of the markers ranged from 0.269 to 0.764, with an average of 0.409. The markers could distinguish among 20 L. edodes cultivars and produced highly repeatable and reproducible results. The markers developed in this study will enable the precise identification of L. edodes cultivars, and may be useful for protecting breeders' rights.

• Asian-Australasian Journal of Animal Sciences
• /
• v.19 no.12
• /
• pp.1702-1705
• /
• 2006

A simulation study was conducted to evaluate a fine-mapping method exploiting population-wide linkage disequilibrium. Data were simulated according to the pedigree structure based on a large paternal half-sib family population with a total of 1,034 or 2,068 progeny. Twenty autosomes of 100 cM were generated with 5 cM or 1 cM marker intervals for all founder individuals in the pedigree, and marker alleles and a number of quantitative trait loci (QTL) explaining a total of 70% phenotypic variance were generated and randomly assigned across the whole chromosomes, assuming linkage equilibrium between the markers. The founder chromosomes were then descended through the pedigree to the current offspring generation, including recombinants that were generated by recombination between adjacent markers. Power to detect QTL was high for the QTL with at least moderate size, which was more pronounced with larger sample size and denser marker map. However, sample size contributed much more significantly to power to detect QTL than map density to the precise estimate of QTL position. No QTL was detected on the test chromosomes in which QTL was not assigned, which did not allow detection of false positive QTL. For the multiple QTL that were closely located, the estimates of the QTL positions were biased, except when the QTL were located on the right marker positions. Our fine mapping simulation results indicate that construction of dense maps and large sample size is needed to increase power to detect QTL and mapping precision for QTL position.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2007.11a
• /
• pp.535-536
• /
• 2007

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.29 no.7B
• /
• pp.613-627
• /
• 2004

In this paper, we propose an Aggregate Fairness Maker (ARM) required for an Edge router to improve fairness of throughput among the flows of Assured Service in DiffServ with different round trip time (RTT) and we propose a user flow Three Color Marker (uf-TCM) as a flow marker that marks packets from the flow as green, yellow, or red. A yellow packet is the packet that consumes loss token in uf-TCM as well as that is demoted green packet in AM due to disobey the aggregate traffic profile. The proposed AFH promotes yellow packet to green packet or demotes green packet to yellow packet through the fair method without per-flow management, and it improves the feirness of throughput among the flows as well as link utilization. A yellow packet and a red packet have the same drop precedence at Core Router in our scheme. So we can use the RIO buffer management scheme. We evaluated the performance of our proposed AFM and the REDP Marker that was proposed to improve fairness without per-flow management. Simulation results show that, compared with the REDP marker, proposed AFM can improve performance of throughput fairness among the flows with different RTT and link utilization under the over-provisioning, exact-provisioning, and under-provisioning network environments at Multiple DiffServ domains as well as at Single DiffServ domain.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.28 no.6B
• /
• pp.588-603
• /
• 2003

In this paper, we propose an Aggregate Three Color Marker without per flow management which is required for an Edge router to improve End-to-End QoS of Assured Service in DiffServ. Proposed Aggregate Three Color Marker is used with the Adaptive RIO-DC scheme to achieve the minimum rate guarantee without per flow management. Assuming that the admission control for Assured Service has been performed, proposed Aggregate Three Color Marker measures incoming In-profile traffic rate at the output link of an edge router using a token-bucket with a token rate equal to the sum of contracted rates of admitted flows passing the edge router. If there are token losses from the token bucket, out-of-profile packets are promoted to Yellow packets within the aggregate traffic profile. And yellow packets are demoted to out-of-profile packets at the input link to an Edge router fer the purpose of fairness maintenance. In-profile packets and Yellow packets are processed identically at the RIO-DC buffer management scheme in our proposed method. Simulation results show that through using proposed Aggregate Three Color Marker with the Adaptive RIO-DC scheme, the minimum rate guarantee for Assured Service can be achieved without per flow management at multiple DiffServ domains.

• The Journal of the Acoustical Society of Korea
• /
• v.18 no.5
• /
• pp.44-51
• /
• 1999

This paper presents a massively parallel computational model for the efficient integration of speech and natural language understanding. The phoneme model is based on continuous Hidden Markov Model with context dependent phonemes, and the language model is based on a knowledge base approach. To construct the knowledge base, we adopt a hierarchically-structured semantic network and a memory-based parsing technique that employs parallel marker-passing as an inference mechanism. Our parallel speech recognition algorithm is implemented in a multi-Transputer system using distributed-memory MIMD multiprocessors. Experimental results show that the parallel speech recognition system performs better in recognition accuracy than a word network-based speech recognition system. The recognition accuracy is further improved by applying code-phoneme statistics. Besides, speedup experiments demonstrate the possibility of constructing a realtime parallel speech recognition system.

• The Journal of Korean Medicine
• /
• v.25 no.3
• /
• pp.45-54
• /
• 2004

Objectives : This study was aimed to evaluate marker substances in Gami-Honghwa-Tang (KH-19) by high performance liquid chromatography-photodiode array detector (HPLC-DAD). Gami-Honghwa-Tang is composed of nine crude herbs, Rehmanniae Radix Preparata, Angelicae Gigantis Radix, Cnidii Rhizoma, Paeoniae Radix, Corni Fructus, Moutan Cortex Radicis, Lycii Fructus, Carthami Flos and Glycyrrhizae Radix. Methods : The separation was performed on an Aquasil C18 (4.6×250mm) column by gradient elution with 0.05% TFA in H2O - 0.05% TFA in acetonitrile (0 min 100:0, 20 min 90:10, 40 min 70:30, 60 min 50:50, 80 min 0:100, 90 min 100:0) as the mobile phase at a flow-rate of 1.0 ml/min with detection at 190-800nm. Also we examined the contents for bacteria, pesticide residue and harmful heavy metals. Results : HPLC-DAD was employed to determine the quantities and the qualities of several marker substances such as 5­hydroxymethyl-2-furaldehyde (5-HMF), paeonol, loganin, paeoniflorin, glycyrrhizin, and decursin in the KH-19. There were no bacterial contents, pesticide residues, or harmful heavy metals. Conclusions : We suggest these results could be a useful evidence for quality control of KH-19. This method permits fingerprints of selected individual marker substances from herbal prescriptions without derivatization, multiple purification steps, or lengthy separation times.

• Horticultural Science & Technology
• /
• v.31 no.1
• /
• pp.72-79
• /
• 2013

Inactivation of the gene coding for dihydroflavonol 4-reductase (DFR) is responsible for the color difference between red and yellow onions (Allium cepa L.). Two inactive DFR-A alleles, DFR-$A^{PS}$ and DFR-$A^{DEL}$, were identified in our previous study. A functional marker was developed on the basis of the premature stop codon that inactivated the DFR-$A^{PS}$ allele. A derived cleaved amplified polymorphic sequences (dCAPS) primer was designed to detect the single nucleotide polymorphism, an A/T transition, which produced the premature stop codon. Digested PCR products clearly distinguished the homozygous and heterozygous red $F_2$ individuals. Meanwhile, to develop a molecular marker for detection of the DFR-$A^{DEL}$ allele in which entire DFR-A gene was deleted, genome walking was performed and approximately 3 kb 5' and 3' flanking sequences of the DFR-$A^R$ coding region were obtained. PCR amplification using multiple primers binding to the extended flanking regions showed that more of the extended region of the DFR-A gene was deleted in the DFR-$A^{DEL}$ allele. A dominant simple PCR marker was developed to identify the DFR-$A^{DEL}$ allele using the dissimilar 3' flanking sequences of the DFR-A gene and homologous DFR-B pseudogene. Distribution of the DFR-$A^{PS}$ and DFR-$A^{DEL}$ alleles in yellow onion cultivars bred in Korea and Japan was surveyed using molecular makers developed in this study. Results showed predominant existence of the DFR-$A^{PS}$ allele in yellow onion cultivars.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.26 no.4B
• /
• pp.413-420
• /
• 2006

The construction of a submerged breakwater has become increasing due to their multiple effects on the coastal zone. Recently, marker rocks have been installed on the submerged breakwater to indicate its position to the vessels instead of buoy systems, since a buoy is not only improper for the ocean view, but also its mooring system may be damaged by the impulsive wave force caused by wave breaking on the breakwater. The accurate estimation of wave forces on such rocks is deemed necessary for their stability design. In this study, the characteristics of irregular wave forces acting on a marker rock, which was installed on a submerged breakwater, was investigated on the basis of laboratory experiments. It was revealed that the dimensionless highest one-third wave force tends to decrease with increasing the installation distance of a marker rock from the leading crown edge of a submerged breakwater. Also, the drag and inertia coefficients for irregular wave forces, which were obtained using the Morison equation, were investigated in relation to K.C. number.

• Animal Bioscience
• /
• v.34 no.9
• /
• pp.1425-1438
• /
• 2021

Maintaining a high level of beef consumption requires paying attention not only to quantitative traits but also to the quality and dietary properties of meat. Growing consumer demands do not leave producers many options for how animals are selected for breeding and animal keeping. Meat and carcass fatness quality traits, which are influenced by multiple genes, are economically important in beef cattle breeding programs. The recent availability of genome sequencing methods and many previously identified molecular markers offer new opportunities for animal breeding, including the use of molecular information in selection programs. Many gene polymorphisms have thus far been analyzed and evaluated as potential candidates for molecular markers of meat quality traits. Knowledge of these markers can be further applied to breeding programs through marker-assisted selection. In this literature review, we discuss the most promising and well-described candidates and their associations with selected beef production traits.