• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2005년도 춘계학술대회 논문집
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• pp.822-827
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• 2005

Recently engineering systems problems become quite large and complicated. For those problems, design requirements are fairly complex. It is not easy to design such systems by considering only one discipline. Therefore, we need a design methodology that can consider various disciplines. Multidisciplinary Design Optimization (MDO) is an emerging optimization method to include multiple disciplines. So far, about seven MDO methodologies have been proposed for MDO. They are Multidisciplinary Feasible (MDF), Individual Feasible (IDF), All-at-Once (AAO), Concurrent Subspace Optimization (CSSO), Collaborative Optimization (CO), Bi-Level Integrated System Synthesis (BLISS) and Multidisciplinary Optimization Based on Independent Subspaces (MDOIS). In this research, the performances of the methods are evaluated and compared. Practical engineering problems may not be appropriate for fairness. Therefore, mathematical problems are developed for the comparison. Conditions for fair comparison are defined and the mathematical problems are defined based on the conditions. All the methods are coded and the performances of the methods are compared qualitatively as well as quantitatively.

• Structural Engineering and Mechanics
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• 제52권1호
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• pp.205-220
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• 2014

This paper is concerned with the modification of multidisciplinary feasible formulation for MDO problems using the integrated coupled approximate models. A drawback of conventional MDFs is the numerical difficulty in decomposing the design variables and deriving the coupled equations of state. To overcome such a drawback of conventional methods, the coupling in analysis and design is resolved by approximating the state variables in each discipline by the response surface method and by modifying the optimization formulation using the corresponding integrated coupled approximate models. The validity, reliability and effectiveness of the proposed method are illustrated and verified through two optimization problems, a mathematical MDF problem and the multidisciplinary optimum design of suspension unit of wheeled armored vehicle.

• Journal of Mechanical Science and Technology
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• 제16권5호
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• pp.609-618
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• 2002

In practical designs, most of the multidisciplinary problems have a large-size and complicate design system. Since multidisciplinary problems have hundreds of analyses and thousands of variables, the grouping of analyses and the order of the analyses in the group affect the speed of the total design cycle. Therefore, it is very important to reorder and regroup the original design processes in order to minimize the total computational cost by decomposing large multidisciplinary problems into several multidisciplinary analysis subsystems (MDASS) and by processing them in parallel. In this study, a new decomposition method is proposed for parallel processing of multidisciplinary design optimization, such as collaborative optimization (CO) and individual discipline feasible (IDF) method. Numerical results for two example problems are presented to show the feasibility of the proposed method.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2005년도 제24회 춘계학술대회논문집
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• pp.11-15
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• 2005

공중발사 방식은 일반적인 지상발사와 비교해 볼 때 많은 장점을 지니고 있다. 그러나 공중발사 로켓의 형상은 모선에 장착 시 많은 제한이 따르기 때문에 여러 해석분야를 통합한 시스템 설계가 필요하다. 시스템 설계는 순차적 최적화와 MDF 기법을 이용하여 수행되었다. 해석 모듈은 임무분석, 단배분, 추진해석, 형상, 중량해석, 공력해석, 궤적해석을 포함한다. 두 가지 기법 중 MDF 기법을 이용하였을 때 더 좋은 결과를 도출하였다. 시스템 최적화 결과 총 중량 1244.91 kg. 위성중량 7.5 kg, 총 길이 6.18m, 지름 0.60 m을 지닌 초음속 공중발사 로켓이 설계되었다.

• 한국항공우주학회지
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• 제33권12호
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• pp.26-32
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• 2005

공중발사 방식은 일반적인 지상발사와 비교해 볼 때 많은 장점을 지니고 있다. 그러나 공중발사 로켓의 형상은 모선에 장착 시 많은 제한이 따르기 때문에 여러 해석분야를 통합한 시스템 설계가 필요하다. 시스템 설계는 순차적 최적화와 MDF 기법을 이용하여 수행되었다. 해석 모듈은 임무분석, 단배분, 추진해석, 형상, 중량해석, 공력해석, 궤적해석을 포함한다. 두 가지 기법 중 MDF 기법을 이용하였을 때 더 좋은 결과를 도출하였다. 시스템 최적화 결과 총 중량 1244.91kg, 위성중량 7.5kg, 총 길이 6.36 m, 지름 0.60m을 지닌 초음속 공중발사 로켓이 설계되었다.

• Journal of Microbiology and Biotechnology
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• 제27권6호
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• pp.1098-1105
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• 2017

Vesicular stomatitis virus G glycoprotein (VSV-G) has been widely used for pseudotyping retroviral, lentiviral, and artificial viral vectors. The objective of this study was to establish a potential approach for large-scale production of VSV-G. To this end, VSV-G was cloned with an N-terminal His-tag into Pichia pastoris expression vector pPIC3.5K. Three clones ($Mut^s$) containing the VSV-G expression cassette were identified by PCR. All clones proliferated normally in expansion medium, whereas the proliferation was reduced significantly under induction conditions. VSV-G protein was detected in cell lysates by western blot analysis, and the highest expression level was observed at 96 h post induction. VSV-G could also be obtained from the condition medium of yeast protoplasts. Furthermore, VSV-G could be incorporated into Ad293 cells and was able to induce cell fusion, leading to the transfer of cytoplasmic protein. Finally, VSV-G-mediated DNA transfection was assayed by flow cytometry and luciferase measurement. Incubation of VSV-G lysate with the pGL3-control DNA complex increased the luciferase activity in Ad293 and HeLa cells by about 3-fold. Likewise, incubation of VSV-G lysate with the pCMV-DsRed DNA complex improved the transfection efficiency into Ad293 by 10% and into HeLa cells by about 1-fold. In conclusion, these results demonstrate that VSV-G could be produced from P. pastoris with biofunctionalities, demonstrating that large-scale production of the viral glycoprotein is feasible.

• 한국항공우주학회지
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• 제31권5호
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• pp.25-36
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• 2003

항공기 설계는 설계 전 분야에 걸친 설계 요소들을 모두 고려한 통합적 환경에서 이루어 져야 한다. 이를 위해 각 분야간의 데이터 공유 및 일관성, 무결성, 최신성 등이 요구되며 이러한 요구사항을 만족할 수 있는 효율적인 데이터베이스의 설계가 필요하다. 데이터베이스의 설계 순기는 저장되고 관리해야 할 데이터의 분석, E-R Diagram의 작성, 테이블 사상으로 이루어진다. 본 연구에서는 상용의 Oracle 8i 데이터베이스 관리시스템을 이용하여 데이터베이스를 설계, 구축하였다. MDF(MultiDisplinary Feasible), IDF(Individual Discipline Feasible), CO(Collaborative Optimization) 등의 MDO(Multidisciplinary Design Optimization) 기법을 적용할 수 있는 데이터베이스의 설계과정을 정립하고, 간단한 수치예제와 무인전투기 최적화 설계 등의 예제를 통하여 통합환경에서의 데이터베이스 설계 방법의 타당성을 검증하였다.

• Journal of Mechanical Science and Technology
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• 제20권1호
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• pp.133-146
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• 2006

Collaborative optimization (CO) is a multi-level decomposed methodology for a large-scale multidisciplinary design optimization (MDO). CO is known to have computational and organizational advantages. Its decomposed architecture removes a necessity of direct communication among disciplines, guaranteeing their autonomy. However, CO has several problems at convergence characteristics and computation time. In this study, such features are discussed and some suggestions are made to improve the performance of CO. Only for the system level optimization, genetic algorithm is used and gradient-based method is used for subspace optimizers. Moreover, response surface models are replaced as analyses in subspaces. In this manner, CO is applied to aero-structural design problems of the aircraft wing and its results are compared with the multidisciplinary feasible (MDF) method and the original CO. Through these results, it is verified that the suggested approach improves convergence characteristics and offers a proper solution.

• 한국항공우주학회지
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• 제40권8호
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• pp.695-702
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• 2012

본 연구에서는 개념설계 단계에서의 해석 결과의 정확도를 높이기 위한 다정밀도 해석과 모든 분야의 요구도를 만족하기 위한 다분야통합 설계 최적화 기법을 적용하였다. 무인항공기의 해석을 위하여 경험식 기반의 저정밀도 해석도구들이 초기 사이징, 공력, 추진, 임무, 중량, 성능, 안정성 도구들로 모듈화되어 개발 및 검증되었다. 개발된 해석도구를 이용하여 설계통합 프로그램을 구성하고, 설계의 정확도를 증가시키기 위하여 다정밀도 해석에 와류 격자법을 이용하였다. 다분야통합 설계 최적화를 위하여 MDF 기법이 적용되었다. 또한 최적화 도구로는 구배기반 최적화 기법을 적용하였다. 제시한 방법의 타당성을 밝히기 위하여, 저정밀도 해석만을 적용한 방법과 다정밀도 해석을 적용한 두 가지 방법의 최적화 결과를 비교하여 본 연구에서 제안된 다정밀도 해석이 개념설계 단계에서 적용 가능함을 보였다.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제23권5호
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• pp.411-422
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• 2020

With the increasing number of children with inflammatory bowel disease (IBD), very early-onset IBD (VEO-IBD), defined as IBD that is diagnosed or that develops before 6 years of age, has become a field of innovation among pediatric gastroenterologists. Advances in genetic testing have enabled the diagnosis of IBD caused by gene mutations, also known as monogenic or Mendelian disorder-associated IBD (MD-IBD), with approximately 60 causative genes reported to date. The diagnosis of VEO-IBD requires endoscopic and histological evaluations. However, satisfactory small bowel imaging studies may not be feasible in this small population. Both genetic and immunological approaches are necessary for the diagnosis of MD-IBD, which can differ among countries according to the available resources. As a result of the use of targeted gene panels covered by the national health insurance and the nationwide research project investigating inborn errors of immunity, an efficient approach for the diagnosis of MD-IBD has been developed in Japan. Proper management of VEO-IBD by pediatric gastroenterologists constitutes a challenge. Some MD-IBDs can be curable by allogenic hematopoietic stem cell transplantation. With an understanding of the affected gene functions, targeted therapies are being developed. Social and psychological support systems for both children and their families should also be provided to improve their quality of life. Multidisciplinary team care would contribute to early diagnosis, proper therapeutic interventions, and improved quality of life in patients and their families.