• 제목/요약/키워드: Multi-species

검색결과 521건 처리시간 0.025초

Effects of Dietary Carbohydrases on Fecal Microbiome Composition of Lactating Sows and Their Piglets

  • Lee, Jeong Jae;Song, Minho;Kyoung, Hyunjin;Park, Kyeong Il;Ryu, Sangdon;Kim, Younghoon;Shin, Minhye
    • Journal of Microbiology and Biotechnology
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    • 제32권6호
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    • pp.776-782
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    • 2022
  • Corn-soybean meal diets are commonly used in the pork industry as a primary source of energy and protein. However, such a diet generally contains non-starch polysaccharides (NSPs) which present a challenge in finding ways to improve their availability and digestibility. Dietary multi-carbohydrases (MCs) have been proposed as an efficient approach to utilize NSPs, and can result in improved growth performance and host intestinal fitness. In this study, we evaluated the effects of MC in lactation diets on gut microbiota composition of lactating sows and their litters. The experimental design contained two dietary treatments, a diet based on corn-soybean meal (CON), and CON supplemented with 0.01% multigrain carbohydrases (MCs). Sow and piglet fecal samples were collected on days 7 and 28 after farrowing. Based on the results from 16S rRNA gene amplicon sequencing, MC led to changes in species diversity and altered the microbial compositions in lactating sows and their piglets. Specifically, the MC treatment induced an increase in the proportions of Lactobacillus in piglets. Clostridium and Spirochaetaceae showed a significantly reduced proportion in MC-treated sows at day 28. Our results support the beneficial effects of dietary carbohydrases and their link with improved production due to better host fitness outcomes and gut microbiota composition.

환경생태정보 전달을 위한 가상·증강현실 사용자 경험디자인 연구 - 서울식물원 온실을 중심으로 - (The User Experience Design of Virtual and Augmented Reality for Environmental and Ecological Information - Focusing on the Conservatory of Seoul Botanic Park -)

  • 조경진;이유미;송영근;정욱주
    • 한국환경복원기술학회지
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    • 제25권2호
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    • pp.69-84
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    • 2022
  • The purpose of this research is to examine the user experience design that effectively exhibits botanical information through a virtual habitat built with 3D modeling and scanning data for the conservatory at Seoul Botanic Park. Seoul Botanic Park's conservatory contains environmental and ecological information on the wide spectrum of diverse plants under twelve cities all over the world. However, the exhibition method, which focuses on maps and information boards, has limitations in delivering diverse plant and habitat information to visitors. Virtual and augmented reality can be used as an effective tool for educating and experiencing the contents of various plant species as it can convey the ecological and environmental conditions of the habitat and local culture at diverse levels. This study experimented with constructing virtual habitats using the Unreal Engine and effectively communicating various botanical information through the interaction. With the introduction of a virtual habitat, we intend to enhance the user experience of park visitors and ultimately explore the possibility of using virtual and augmented reality to convey multi-layered environmental and ecological information of landscape.

다중주파수를 이용한 살오징어(Todarodes pacificus)의 분포밀도 추정 (Density Estimation of Japanese Common Squid Todarodes pacificus Using Multi-frequency)

  • 신형호;정종일;이형빈;오우석;박근창;이경훈
    • 한국수산과학회지
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    • 제54권6호
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    • pp.1023-1029
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    • 2021
  • The Japanese common squid Todarodes pacificus is one of the fish species within the total allowable catch (TAC) system which requires further investigation. In this study, the acoustic survey method was used to analyze the distribution of the Japanese common squid Todarodes pacificus across all the seas of South Korea. The sea area within Korea was investigated using the research vessels 20, 21, and 22 of the National Institute of Fisheries Science. The acoustic surveys were carried out from July to September 2019 and February to May 2020. The acoustic systems used in the survey had frequencies of 38 kHz and 120 kHz (EK60, EK80, Simrad, Norway) of the split-beam scientific echosounder. The results showed that, in spring, 277 m2/nmi2 was the highest in the east sea area, and the same in the summer season 880 m2/nmi2 was the highest in the east sea area. In autumn, the highest nautical area scattering coefficient (NASC) value was observed in the coastal portion of the south sea, and in winter, the NASC values were generally low in all the sea area.

236U accelerator mass spectrometry with a time-of-flight and energy detection system

  • Li Zheng;Hiroyuki Matsuzaki;Takeyasu Yamagata
    • Nuclear Engineering and Technology
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    • 제54권12호
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    • pp.4636-4643
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    • 2022
  • A time-of-flight and energy (TOF-E) detection system for the measurement of 236U accelerator mass spectrometry (AMS) has been developed to improve the 236U/238U sensitivity at Micro Analysis Laboratory, Tandem accelerator (MALT), The University of Tokyo. With observing TOF distribution of 235U, 236U and 238U, this TOF-E detection system has clearly separated 236U from the interference of 235U and 238U when measuring three kinds of uranium standards. In addition, we have developed a novel method combining kernel-based density estimation method and multi-Gaussian fitting method to estimate the 236U/238U sensitivity of the TOF-E detection system. Using this new estimation method, 3.4 × 10-12 of 236U/238U sensitivity and 1.9 ns of time resolution are obtained. 236U/238U sensitivity of TOF-E detection system has improved two orders of magnitude better than that of previous gas ionization chamber. Moreover, unknown species other than uranium isotopes were also observed in the measurement of a surface soil sample, which has demonstrated that TOF-E detection system has a higher sensitivity in particle identification. With its high sensibility in mass determination, this TOF-E detection system could also be used in other heavy isotope AMS.

Profiles of Non-aureus Staphylococci in Retail Pork and Slaughterhouse Carcasses: Prevalence, Antimicrobial Resistance, and Genetic Determinant of Fusidic Acid Resistance

  • Yang, Yu Jin;Lee, Gi Yong;Kim, Sun Do;Park, Ji Heon;Lee, Soo In;Kim, Geun-Bae;Yang, Soo-Jin
    • 한국축산식품학회지
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    • 제42권2호
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    • pp.225-239
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    • 2022
  • As commensal colonizers in livestock, there has been little attention on staphylococci, especially non-aureus staphylococci (NAS), contaminating meat production chain. To assess prevalence of staphylococci in retail pork and slaughterhouse carcass samples in Korea, we collected 578 samples from Korean slaughterhouses (n=311) and retail markets (n=267) for isolation of staphylococci and determined antimicrobial resistance phenotypes in all the isolates. The presence of and prevalence of fusB-family genes (fusB, fusC, fusD, and fusF) and mutations in fusA genes were examined in fusidic acid resistant isolates. A total of 47 staphylococcal isolates of 4 different species (Staphylococcus aureus, n=4; S. hyicus, n=1; S. epidermidis, n=10; Mammaliicoccus sciuri, n=32) were isolated. Fusidic acid resistance were confirmed in 9/10 S. epidermidis and all of the 32 M. sciuri (previously S. sciuri) isolates. Acquired fusidic acid resistance genes were detected in all the resistant strains; fusB and fusC in S. epidermidis and fusB/C in M. sciuri. Multi-locus sequence type analysis revealed that ST63 (n=10, 31%) and ST30 (n=8, 25%) genotypes were most prevalent among fusidic acid resistant M. sciuri isolates. In conclusion, the high prevalence of fusB-family genes in S. epidermidis and M. sciuri strains isolated from pork indicated that NAS might act as a reservoir for fusidic acid resistance gene transmissions in pork production chains.

선형 예측 분석 기반의 딱총 새우 잡음 검출 기법 (Linear prediction analysis-based method for detecting snapping shrimp noise)

  • 박진욱;홍정표
    • 한국음향학회지
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    • 제42권3호
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    • pp.262-269
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    • 2023
  • 본 논문에서는 선형 예측 분석을 기반으로 한 딱총새우 잡음 검출을 위한 특징을 제안한다. 딱총새우는 천해에 서식하는 종으로, 높은 진폭의 신호를 생성하고 빈번하게 발생하기 때문에 수중 잡음의 주된 원인 중 하나이다. 제안된 특징은 딱총새우 잡음이 갑작스럽게 발생하고 빠르게 소멸하는 특징을 활용하기 위해 선형 예측 분석을 이용하여 정확한 잡음 구간을 검출하고 딱총새우 잡음의 영향을 줄인다. 선형 예측 분석으로 예측한 값과 실제 측정값 사이의 오차가 크기 때문에 이를 통해 효과적으로 딱총새우 구간 검출이 가능해진다. 추가적으로 제안된 특징에 일정 오경보 확률 탐지기를 결합하여 잡음 구간 검출 성능을 추가적으로 개선한다. 제안한 방법을 딱총새우 잡음 구간 검출 최신 방법으로 알려진 다층 웨이블릿 패킷 분해와 비교한 결과, 제안한 방법이 수신자 조작 특성 곡선과 곡선 아래의 면적 측면에서 성능이 평균적으로 0.12만큼 우수하였고 계산량 측면에서도 계산 복잡도가 더 낮았다.

Effect of Silencing subolesin and enolase impairs gene expression, engorgement and reproduction in Haemaphysalis longicornis (Acari: Ixodidae) ticks

  • Md. Samiul Haque;Mohammad Saiful Islam;Myung-Jo You
    • Journal of Veterinary Science
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    • 제25권3호
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    • pp.43.1-43.13
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    • 2024
  • Importance: Haemaphysalis longicornis is an obligate blood-sucking ectoparasite that has gained attention due its role of transmitting medically and veterinary significant pathogens and it is the most common tick species in Republic of Korea. The preferred strategy for controlling ticks is a multi-antigenic vaccination. Testing the efficiency of a combination antigen is a promising method for creating a tick vaccine. Objective: The aim of the current research was to analyze the role of subolesin and enolase in feeding and reproduction of H. longicornis by gene silencing. Methods: In this study, we used RNA interference to silence salivary enolase and subolesin in H. longicornis. Unfed female ticks injected with double-stranded RNA targeting subolesin and enolase were attached and fed normally on the rabbit's ear. Real-time polymerase chain reaction was used to confirm the extent of knockdown. Results: Ticks in the subolesin or enolase dsRNA groups showed knockdown rates of 80% and 60% respectively. Ticks in the combination dsRNA (subolesin and enolase) group showed an 80% knockdown. Knockdown of subolesin and enolase resulted in significant depletion in feeding, blood engorgement weight, attachment rate, and egg laying. Silencing of both resulted in a significant (p < 0.05) reduction in tick engorgement, egg laying, egg hatching (15%), and reproduction. Conclusions and Relevance: Our results suggest that subolesin and enolase are an exciting target for future tick control strategies.

월성 원자력발전소 주변해역에 서식하는 대형저서동물의 군집구조 (Community Structure of Macrobenthic Assemblages around the Wolseong Nuclear Power Plant, East Sea of Korea)

  • 서인수;문형태;최병미;김미향;김대익;윤재성;변주영;최휴창;손민호
    • 환경생물
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    • 제27권4호
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    • pp.341-352
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    • 2009
  • 본 연구는 월성 원자력발전소 주변해역에 서식하는 대형저서동물의 출현 종수, 생물량 및 군집구조를 파악하기 위하여 2007년 10월부터 2008년 7월까지 계절별로 현장조사를 실시하였다. 총 163종의 대형저서동물이 출현하였고, 단위면적당 ($m^2$) 개체수와 생체량은 각각 1,005개체와 21.81 gWWt이었다. 출현 개체수의 자료를 기초로 LeBris index (1988)를 이용하여 상위 10위까지의 우점종을 선정하였다. 개체수에 근거한 상위 10위 우점종들은 전체 출현 밀도의 69.00%를 점유하였고, 중요 우점종은 다모류의 민얼굴갯지렁이(Spiophanes bombyx), Mediomastus californiensis, 투구갯지렁이(Sigambra tentaculata), 양손갯지렁이(Magelona japonica), 긴자락송곳갯지렁이(Scoletoma longifolia) 및 절지동물문 갑각류의 미동정 옆새우류(Amphipoda spp.) 등이었다. 출현 종의 개체수 자료를 기초로 다변량분석(집괴분석과 다차원배열법)을 실시한 결과, 크게 2개의 주정점군을 포함하여 1개의 소정점군(추계의 정점 9와 동계의 정점 3) 및 3개의 개별 정점(춘계의 정점 4 및 하계의 정점 6과 9)으로 구분되었다. 이 중 다수의 정점들이 포함된 주정점군 1은 모래함량이 우세한 정점들로, 주정점군 2는 펄함량이 상대적으로 높은 환경적 특성을 나타내었다. 특히 모래 함량이 높은 정점군에서는 다모류의 Protodorvillea egena와 이매패류의 개량조개(Mactra chinensis)와 꼬마보라 맛조개(Siliqua pulchella)가 우점한 반면, 펄함량이 많은 정점군에서는 다모류의 M. californiensis, 양손갯지렁이, 오뚜기갯지렁이 (Sternaspis scutata), 긴자락송곳갯지렁이와 이매패류의 말발조개(Thyasira tokunagai)와 아기반 투명조개(Theora fragilis)의 밀도가 높았다. 본 연구해역에 출현한 대형저서동물 군집의 서식기질, 수심 및 온배수 배출에 따른 개체수의 차이유무를 파악하기 위하여 일원일차분산분석을 실시한 결과, 본 연구해역의 대형저서동물 군집은 상기의 무기 환경요인에 대하여 통계적으로 유의한 차이가 없었다(p>0.05).

구강편평세포암종 세포에서 감초 유래 Liquiritigenin의 항증식, 세포주기 정지 및 세포사멸 유도 (Anti-proliferation, Cell Cycle Arrest, and Apoptosis Induced by Natural Liquiritigenin from Licorice Root in Oral Squamous Cell Carcinoma Cells)

  • 곽아원;윤구;채정일;심정현
    • 생명과학회지
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    • 제29권3호
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    • pp.295-302
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    • 2019
  • Liquiritigenin (LG)은 licorice 뿌리에서 분리된 chiral flavonoid이다. LG는 항산화, 항암 및 항염증 효과를 포함하여 다양한 생물학적 활성을 가지고 있다. 구강편평세포암종에서 LG의 항암 활성은 아직 밝혀지지 않았다. 본 연구에서는 구강편평상피암 세포(HN22)에서 LG의 항암 효능을 시험하였다. HN22 세포에 LG를 처리하여 MTT 분석으로 세포 생존율을 평가하였으며, Annecin V/7-Aminactinomycin D 염색, 세포주기 및 Multi-caspase 활성을 $Muse^{TM}$ cell Analyzer로 분석하여 세포사멸 유도를 확인하였다. 분석결과, 구강편평상피암 HN22 세포에 LG를 처리시 G2/M 세포주기 정지를 일으켰으며, Western blotting 통하여 cyclin B1 및 CDC2 발현 감소와 p27 발현 증가를 확인하였다. LG는 활성산소종의 생성을 유발하고, CCAAT/enhancer-binding protein homologous protein (CHOP) 및 78-kDa glucose regulated protein (GRP78)의 발현을 농도의존적으로 유도하였다. HN22 세포에 LG의 처리는 미토콘드리아 막전위의 손실(${\Delta}{\Psi}m$)을 일으켰다. LG를 처리한 HN22 세포의 단백질 분석결과 apoptotic protease activating factor-1 (Apaf-1), cleaved Poly (ADP-Ribose) Polymerase (C-PARP) 및 Bax의 발현을 증가함을 확인하였다. 따라서 우리의 결과는 LG이 구강편평상피암 세포의 세포사멸을 유도하여 항암제 역할을 할 수 있는 천연 화합물임을 시사한다.

한국(韓國)에 있어서의 장내기생충감염(腸內寄生虫感染)의 현황(現況)과 추이(推移) (Current Status and Transition of the Prevalence of Intestinal Parasitic Infections in Korea)

  • 김동찬
    • 농촌의학ㆍ지역보건
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    • 제9권1호
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    • pp.83-108
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    • 1984
  • Out of a total of 58 species of helminthic and protozoan parasitic infections in Korea, so far 38 species were reported as intestinal parasites of man. Quite a few species of the intestinal parasitic infections have long been prevalent throughout the country and this has been a significant public health problem. In this paper, current status and transition of the intestinal parasitic infections in the past years were presented. Chronological reviewing of data show background and prospects of change in the prevalence of infections. In the national prevalence survey on parasitic infections conducted once every five years since 1971, stool examinations were done by both cellophane thick smear and brine flotation techniques. Every egg positive case of Ascaris lumbricoides and Clonorchis sinensis was further examined by Stoll's dilution egg counting technique. In 1981, perianal swab using adhesive cellulose tape was added for Enterobius infection. For protozoan cyst examination conducted by province and city in '81, fecal specimens were fixed in SAF solution and examined by the formalin-ether concentration technique. High prevalence of parasitic infection in ana before the 1960s can be easily understood from the data given by the Ministry of Health and Social Affairs in 1967. From a parasite control point of view, the 1960s was the preparatory period particularly for control of soil-transmitted helminthiasis. Several organizations which have contributed to parasite control were founded in this period and the prevention law of parasitic infections was passed in '66. In the '70s, overall prevalence rates of the common intestinal parasitic infections, which were highly prevalent in the past were turned into reduction phase for the first time. The '80s can be said to be an active control period of parasitic infections. Intestinal helminths According to the reports of the national prevalence survey on parasitic infections, the prevalence of helminthic infections was 84.3%(number of person examined:24,887) in '71, 63.2%(27,178) in '76, and 41.1%(35,018) in '81. By area, the prevalence rate in '81 was 35.1%(20,569) in urban areas and 49.6%(14,447) in rural areas. Intestinal nematodes Ascaris lumbricoides The prevalence of Ascaris infection has decreased significantly in recent years. Among students, the prevalence was 55.4%, in '69 and decreased to 4.7% in '83. In the national prevalence surveys, the prevalence decreased to 13.0% in '81 from 54.9% in '71. By area, the prevalence decreased to 8.5% in '81 from 46.4% in '71 in urban area and 19.4% in '81 from 59.6% in '71 in rural area. By age, the prevalence has become in recent years relatively even in all age groups, although higher prevalence used to be seen in young age groups of around 10 years old, particulary in the highly prevalent rural areas. By sex, the prevalence was higher in the female than in the male. Unfertilized egg positive rates among the ascariasis cases increased gradually up to 55.4% on the average in '81. The intensity of the infection was also significantly decreased. Trichuris trichiura Trichuris infection had also decreased to 23.4% in '81 from 65.4% in '71. By area, the decreasing tendency of the prevalence became faster in urban areas than in the rural areas. The prevalence in urban and rural areas in '71 was 69.7%, and 63.1% respectively and decreased to 19.5% and 29.0% respectively in '81. By age, the prevalence reached a peak at the 10-14 age group and showed relatively even distribution throughout all age groups. By sex, the prevalence was close in young age groups, but in the 30s or over age group, especially in rural area, the prevalence was significantly higher in the female than in the male. The prevalence has much fluctuated depending in the area. The prevalence in rural areas surveyed in the '80s shows a range between 20.9% and 73.7% by locality. It is anticipated that the prevalence of Trichuris infection will drop more rapidly, when mass treatment is conducted. Hookworms Hookworm infection by mostly Ancylostoma duodenale and a few by Necator americanus has decreased to a negligible levels in recent years. In the national prevalence surveys, the prevalence was 10.7% in '71, 2.2% in '76, and 0.5% in '81. The prevalence was higher in rural areas than in urban areas. Wide application of multi-specific anthelminthics in the ascariasis control programmes conducted in the past decade appear to have been effective against hookworm infection. Trichostrongylus orientalis As in the case with hookworm infection, the prevalence of Trichostrongylus infection has reached a negligible levels. In the national prevalence surveys, the prevalence was 7.7% in '71, 1.0% in '76 and 0.2% in '81. Enterobius vermicularis In the national prevalence survey in '81, the egg positive rate was 12.0%. Higher prevalence is expected when examined repeatedly. The prevalence rate was 10.3% in urban area and 14.6% in rural area. In recent surveys conducted in rural areas among schoolchildren, the prevalence was 32.4% in Gimhae Gun in '82 and 64.1% in Yeongyang Gun in '83. By age, the egg positive rate was higher in young age groups of around 10 and sharply decreased in age groups of around 20 and then somewhat increased again in middle age groups. By sex, the prevalence was higher in the female than in the male. Strongyloides stercoralis Strongyloides stercoralis infection has rarely been found in Korea. Three cases were reported in 1914. And 0.1-0.5% were found infected out of 2,642 persons examined at the prisoner-of-war camp on Geojedo in 1956. One case was reported in '54 and '82, respectively. Anisakis spp. No systematic survey has been conducted for anisakiasis In Korea. So far, only several cases have been found 1 case in Seoul in '71, 5 cases in Busan in '81 and 1 case in Busan in '84. Intestinal trematodes Metagonimus yokogawai In the national prevalence survey conducted in 1981, the egg positive rate was 1.24% on the average. High endemic areas are located in the southwestern part of Korea. The prevalence in Hadong Gun was 29.1% on the average in '79. In a survey conducted in 76, the prevalence was 44.0% in Gwangyang, 55.0% in Gogseung and 29.0% in Gurye. The infection is closely correlated with raw sweetfish consumption in these areas. Other intestinal trematodes A human case of Heterophyes heterophyes was reported in 1914. Several species were reported in the '80s : 17 cases of Fibricola seoulensis, 9 cases of Pygidiopsis summa, 8 cases of Heterophyes heterophyes nocens, 1case of Heterophyopsis continua, 2 cases of Stellantchasmus falcatus, 1 case of Stictodora sp., 1 case of Echinostoma hortense, and 4 cases of Echinochasmus japonicus. As the intermediate hosts, snakes and frogs play a role for F.seoulensis and fish for the rest of the species. Intestinal cestodes Taenia saginata and T. solium Egg positive rates in the national prevalence survey were 0.7% in '76 and 1.1% in '81. The prevalence in '81 was 0.6% in urban area and 1.8% in rural area. The proglottid positive rate in Jeju Do was 19.2% on the average. On Udo, Jeju Do in 1983, the egg positive rate among the inhabitants was 2.9%. Hymenolepis nana In the national prevalence survey, egg positive rates were 0.6% in '76 and 0.4% in '81. No difference was seen in the prevalence by area and sex. Hymenolepis diminuta Infected cases were reported : 3 in '64 and I in '66. Egg positive rate in '81 was 0.01% in the national prevalence survey. Diphylobothrium latum So far, about 30 cases have been reported. The cases have been reported more frequently in recent surveys. Mesocestoides sp. A case was reported from a hospitalized patient in Seoul in '67. Spirometra erinacei Two cases were reported in '84 following reidentification of the adult worms collected in '74. Intestinal protozoa Out of a total of 23 species of human protozoan infections in Korea, 13 species were reported as intestinal protozoa : Entamoeba histolytica, E coli, Endolimax nana, Iodamoeba b$\ddot{u}$tschlii, Dientamoeba fragilis, Giardia lamblia, Chilomastix mesnilii, Embadomonas sp., Enteromonas hominis, Trichomonas hominis, Isospora belli, I. Hominis(Sarcocystis hominis), and Balantidium coli. Since the first report on intestinal protozoan infections in 1925, there have been quite a few survey data on the prevalence of the infection. It was found reviewing the data chronologically that up to the early '70s the infection was prevalent around a 30-50%. After that, the protozoan cyst positive rate has shown the tendency of gradual decrease throughout the country. Protozoan cyst survey conducted in Seoul and several provinces in 1981 revealed infection rates of 8.9%(1,310) in Gangweondo, 10.7%(1,703) in Gyenggi Do, 11.7%(1,032) in Jeonra Buk Do, 9.1%(4,116) in Jeonra Nam Do, and 1.4%(5,275) in Seoul. Entamoeba histolytica In the survey conducted by province in '81, the cyst positive rate was 0.8% in Gangweon-do, 0.3% in Gyeonggi Do, 1.4% in both Jeonra Buk Do and Jeonra Nam Do, and 0.2% in Seoul. Giardia lamblia In the survey by province in '81, cyst positive rates were 2.2% in both Gyeonggi Do and Jeonra Buk Do, 1.9% in Jeonra Nam Do, 0.5% in Gangweon Do, and 0.9% in Seoul. Balantidium coli Two cases were reported. One in 1930 and the other in '74. Isospora belli and I. Hominis(Sarcocystis hominis) Isospora belli was reported : 1 case in '56 and 3 cases in '66. I. Hominis, recently identified to be synonymous with Sarcocystis hominis, was reported : 3 cases in '66. Other intestinal protozoa The protozoan parasites other than the above mentioned are generally treated as commensal, although some of them are considered to be pathogenic. The data of '81 show that about 10% of the inhabitants are still infected with protozoa.

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