• Title/Summary/Keyword: Multi-species

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Molecular Characterization of a Transient Expression Gene Encoding for 1-Aminocyclopropane-1-carboxylate Synthase in Cotton (Gossypium hirsutum L.)

  • Wang, Xia;Zhang, Ying;Zhang, Jiedao;Cheng, Cheng;Guo, Xingqi
    • BMB Reports
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    • v.40 no.5
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    • pp.791-800
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    • 2007
  • Ethylene performs an important function in plant growth and development. 1-aminocyclopropane-1-carboxylate (ACC) synthase (ACS), the key enzyme involved in ethylene biosynthesis, has been the focus of most ethylene studies. Here, a cotton ACS gene referred to as Gossypium hirsutum ACS1 (GhACS1), was isolated. The full-length cDNA of GhACS1 encodes for a 476-amino acid protein which harbors seven conserved regions, 11 invariant amino acid residues, and the PLP binding active site, all of which characterize ACC synthases. Alignment analysis showed that GhACS1 shared a high degree of identity with other known ACC synthases from different species. Two introns were detected in the genomic DNA sequence, and the results of Southern blot analysis suggested that there might be a multi-gene family encoding for ACC synthase in cotton. From the phylogenetic tree constructed with 24 different kinds of ACC synthases, we determined that GhACS1 falls into group II, and was closely associated with the wound-inducible ACS of citrus. The analysis of the 5' flanking region of GhACS1 revealed a group of putative cis-acting elements. The results of expression analysis showed that GhACS1 displayed its transient expression nature after wounding, abscisic acid (ABA), and $CuCl_2$ treatments. These results indicate that GhACS1, which was transiently expressed in response to certain stimuli, may be involved in the production of ethylene for the transmission of stress signals.

Dispersion and Migration of Potentially Toxic Elements in the Rock-Soil-Plant System from the Boeun Area Underlain by Black Shales, Korea (보은지역 흑색셰일 분포지역에서의 암석-토양-식물계내 잠재적 독성원소들의 분산과 이동)

  • Lee, Jin-Soo;Chon, Hyo-Taek;Kim, Kyoung-Woong
    • Economic and Environmental Geology
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    • v.30 no.6
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    • pp.587-601
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    • 1997
  • This study had three purposes: (1) to investigate the enrichment levels and dispersion patterns of potentially toxic elements in the rock-soil-plant system; (2) to evaluate the uptake ratios of heavy metals from soils into plants and (3) to assess the chemical speciation of heavy metals in soils. Rock, surface soil and plant samples were collected in the Boeun area underlain by black shales of the Okchon Zone. These samples were analyzed for multi-elements using INAA, ICP-AES and AAS. The maximum abundance of U in black shales is 16 mg/kg and radioactivity counts up to 300 cpm. In particular, Mo, V, Ba, Cd, Pb and U are enriched in black shales. Most of soils derived from black shales show high concentrations of U, As, Mo, Ba, Cu, Cd, Pb, Zn and mean concentrations of As and Mo in soils (20 mg/kg of As and 6.6 mg/kg of Mo) are higher than the permissible level suggested by Kloke (1979). Enrichment index values of soils are calculated and higher than 1.0 in the black shale area with the highest value of 6.4. Mean concentration of Cd in plants is higher than those of Cu, Pb and Zn. The concentration of Cd in plant species decreases in the order of Chinese cabbage > red pepper > soybean=sesame > rice stalk > com > rice grain. The biological absorption coefficients (BAC) in plants are in the order of Cd > Zn=Cu > Pb, which suggests that Cd is more bioavailable to plants than Cu, Pb and Zn. From the results of sequential extraction analysis of soils, relatively high proportion of Cu, Pb and Zn are present as residual fractions whereas that of Cd as non-residual fractions. Cadmuim occurs predominantly as exchangeable/water-acid soluble phase in soils, and Cd is more mobile and bioavailable than Cu, Pb and Zn.

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Plant Community Structure of Daetjae(hill)~Baekbongryung(ridge), the Baekdudaegan Mountains (백두대간 댓재에서 백봉령구간 마루금의 식물군집구조 특성)

  • Lee, Soo-Dong;Hong, Suk-Hwan;Kim, Ji-Suk
    • Korean Journal of Environment and Ecology
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    • v.26 no.5
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    • pp.719-729
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    • 2012
  • Baekdudan has 670km long within South Korea, so the study for detail vegetation characteristics is needed. In this study, we surveyed the plant community structure from Daetjae to Baekbongryung for the next restoration and management plan. We designated 40 quadrats ($10m{\times}10m(100m^2)$ for this study. As a result of TWINSPAN, plant community separated 8 different communities such as Abies koreana comm., Pinus densiflora comm., Quercus mongolica comm. and Larix kaempferi comm. etc. Abies koreana comm. and Pinus densiflora comm. which is mainly located in the mountain ridge and near rocks are needed avoidance from the competition with Quercus mongolica comm. The possibility of atrophy of these communities is to be high, the protection is needed. Species diversity index was between 0.8046~1.1283. Most communities have multi-layer structure and have the ecological value of protection.

Analysis of Hydraulic Characteristics of Yeongsan River and Estuary Using EFDC Model (EFDC-NIER 모델을 이용한 영산강 하구 물흐름 특성 분석)

  • Shin, Chang Min;Kim, Darae;Song, Yongsik
    • Journal of Korean Society on Water Environment
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    • v.35 no.6
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    • pp.580-588
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    • 2019
  • The flow of the middle and downstream of the Yeongsan River is stagnant by two weirs of Seungchon and Juksan and the estuary dam and maintained in freshwater. In this study, the Environmental Fluid Dynamics Code-National Institute of Environment Research(EFDC-NIER) model was applied to the Yeongsan River to simulate water flow, temperature, and salinity stratification. The EFDC-NIER model is an improved model which can simulate multi-functional weirs operation, multiple algal species, and the vertical movement mechanism of algal based on the EFDC model. The simulation results for the water level, water temperature, velocity, and salinity reproduced the observed values well. The mean absolute error(MAE) of the model calibration in the annual variations of the water level was 0.1-0.3 m, water temperature was 0.8-1.7 ℃, velocity was 4.5-7.1 cm/sec, and salinity was 1.5 psu, respectively. In the case of scenario simulation for the full opening of the estuary dam, the water level of the estuary dam was directly impacted by the tide so it was predicted to rise - 1.35 m to 0.2 m on average sea level. The velocity was also predicted to increase from 2.7 cm/sec to 50.8 cm/sec, and the flow rate to increase from 53 ㎥/sec to 5,322 ㎥/sec.

Estimation and Characteristics of Atmospheric Deposition Flux of Polycyclic Aromatic Hydrocarbons (PAHs) into the Masan and Haengam Areas of Korea (마산과 행암 지역으로 유입되는 다환방향족탄화수소(PAHs)의 대기 침적 플럭스 산정과 특성)

  • Lee Su-Jeong;Moon Hyo-Bang;Choi Min-kyu
    • Journal of Environmental Science International
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    • v.15 no.2
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    • pp.121-131
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    • 2006
  • Atmospheric bulk (wet and dry) samples were monthly collected in Masan and Heangam areas of Korea, to assess the deposition flux and seasonal variation of polycyclic aromatic hydrocarbons (PAHs). Deposition fluxes of PAHs in bulk samples were determined using gas chromatography coupled to mass spectrometer detector (GC/MSD). Particle deposition fluxes from Masan and Haengam areas varied from 13 to $87\;g/m^2/year$ and from 5 to $52\;g/m^2/year$, respectively. PAHs deposition fluxes in atmospheric bulk samples in Masan and Haengam areas ranged from 135 to $464\;{\mu}g/m^2/year$ and from 62.2 to $194\;{\mu}g/m^2/year$, respectively. Atmospheric deposition fluxes of particles and PAHs in this study were comparable to or slightly lower values than those from different locations in Korea and other countries. PAHs profiles of atmospheric deposition bulk samples showed slightly different from two sampling areas, however the predominant species of PAHs were similar. Indeno (1,2,3-c,d)pyrene, benzo(g,h,i)perylene, phenanthrene compounds were the most detected PAHs in deposition bulk samples. Carcinogenic PAHs occupied the contribution of approximately $30-40\%$ of the total PAHs deposition fluxes. The non-metric multi-dimensional scaling (MDS) was used, to assess the differentiation of PAHs source between two sampling areas. The result suggests that PAHs contamination sources were different according to the location and season surveyed. There was no an apparent relationship between the PAHs deposition flux against temperature and rainfall amount, even though summer season with the highest temperature and the largest amount of precipitation showed the lowest PAHs deposition flux. Benzo(e)pyrene/benzo(a)pyrene ratio indicated that the photo-degradation process was one of important factors to the seasonal variation of PAHs with the lower deposition fluxes.

Colonization and community changes in benthic macroinvertebrates in Cheonggye Stream, a restored downtown stream in Seoul, Korea

  • Shin, Il-Kwon;Yi, Hoon-Bok;Bae, Yeon-Jae
    • Journal of Ecology and Environment
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    • v.34 no.2
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    • pp.175-191
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    • 2011
  • Colonization patterns and community changes in benthic macroinvertebrates in the Cheonggye Stream, a functionally restored stream in downtown Seoul, Korea, were studied from November 2005 to November 2007. Benthic macroinvertebrates were quantitatively sampled 15 times from five sites in the stream section. Taxa richness (59 species in total) increased gradually over the first year, whereas the density revealed seasonal differences with significantly lower values in the winter season and after flood events. The benthic macroinvertebrate fauna may have drifted from the upstream reaches during floods and from the Han River, arrived aerially, or hitchhiked on artificially planted aquatic plants. Oligochaeta, Chironommidae, Psychodidae, and Hydropsychidae were identified as major community structure contributors in the stream. Swimmers and clingers colonized relatively earlier in the upper and middle reaches, whereas burrowers dominated particularly in the lower reaches. Collector-gatherers colonized at a relatively early period throughout the stream reaches, and collector-filterers, such as the net-spinning caddisfly (Cheumatopyche brevilineata), predominated in the upper and middle reaches after a 1-year time period. Cluster analyses and multi-response permutation procedures demonstrated that the Cheonggye Stream shares more similarities with the Jungnang Stream than with the Gapyeong Stream. Detrended correspondence analysis and nonmetric multidimensional scaling demonstrated that physical environmental factors (depth, current velocity, dissolved oxygen, and pH) as well as nutrients (total nitrogen and total phosphorous), water temperature, and conductivity could affect the distribution of benthic macroinvertebrates in the study streams.

Vegetation Rehabilitation and Management Strategy of the fired Woodland in Chesuk-bong of Mt. Chiri (지리산(智異山) 제석봉(帝釋峰) 산화적지(山火跡地)의 식생회복(植生回復) 및 관리방안(管理方案))

  • Chung, Jae-Min;Moon, Hyun-Shik;Ma, Ho-Seop
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.2 no.1
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    • pp.58-65
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    • 1999
  • The purpose of this study was to evaluate the effect of rehabilitation measures on the fired woodland of Chesuk-bong in Mt. Chiri through the vegetation structure and comparison of growth pattern between natural seedlings and transplanted Abies koreana seedlings. It was also discussed the rate of standing and fallen trees of dead conifers in the management strategy for vegetation regeneration and protection of sub-alpine area. In this fired woodland, shrub layers consisting of Weigela subsessilis, Sambucus williamsii var. coreana, Rhododendron schlippenbachii, R. mucronulatum, Tripterygium regelii, and etc. were gradually expanded, and tree species such as Betula ermani, Sorbus commixta, Acer pseudosieboldianum, and Malus baccata var. mandshurica were also regenerated. The growth of height and crown width of Abies koreana seedlings transplanted for vegetation regeneration were a little lower than those of natural seedlings. The seedlings of A. koreana transplanted in this fired woodland grew about 50.6% normally, but the others had multi-branched or growth stopped by death of terminal shoot. Because the number of dead conifers by fire tend to be gradually increased as time passed, it can occurs to the soil erosion and landslide by weakness of the cohesion and resistance of soil. Therefore, it is consider that rehabilitation measures projects of the fired woodland in subalpine area may be more prevention a natural disaster like soil erosion, flood and landslide.

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A Study on the Source Profile Development for Fine Particles (PM2.5) Emitted from Biomass Burning (Biomass-burning에서 배출되는 미세입자 (PM2.5)의 배출원 구성물질 성분비 개발에 관한 연구)

  • Kang, Byung-Wook;Lee, Hak-Sung
    • Journal of Korean Society for Atmospheric Environment
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    • v.28 no.4
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    • pp.384-395
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    • 2012
  • This study was performed to develop the source profiles for fine particles ($PM_{2.5}$) emitted from the biomass burning. The multi-method research strategy included a usage of combustion devices such as field burning, fireplace, and residential wood burning to burn rice straw, fallen leaves, pine tree, and oak tree. The data were collected from multiple sources and measured water-soluble ions, elements, elemental carbon (EC), and organic carbon (OC). From this study, it turned out that OC (34~67%) and EC (1.2~39%) are the major components emitted from biomass burning. In the case of burning rice straw at field burning, OC (66.6%) was the most abundant species, followed by EC (4.3%), $Cl^-$ (3.6%), Cl (2.1%), and $SO^{2-}_4$(1.9%). Burning rice straw, fallen leaves, pine tree, and oak tree at fireplace, the amount of OC was 58.5%, 52.7%, 52.5%, and 61.2%, and that of EC was 1.2%, 18.4%, 36.5%, and 2.7%, respectively. The ratio of OC for the burning of pine tree and oak tree from the residential wood burning device was 56.9% and 34.3%, and that of EC was 25% and 38.6%, respectively. Applying the measured data with respect to the proportion of components emitted from biomass burning to reference model, it turned out that self-diagnosed result was appropriate level, and the result based on the model is in highly corresponding to actual timing of biomass burning.

Identification of Neuroactive Constituents of the Ethyl Acetate Fraction from Cyperi Rhizoma Using Bioactivity-Guided Fractionation

  • Sim, Yeomoon;Choi, Jin Gyu;Gu, Pil Sung;Ryu, Byeol;Kim, Jeong Hee;Kang, Insug;Jang, Dae Sik;Oh, Myung Sook
    • Biomolecules & Therapeutics
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    • v.24 no.4
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    • pp.438-445
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    • 2016
  • Cyperi Rhizoma (CR), the rhizome of Cyperus rotundus L., exhibits neuroprotective effects in in vitro and in vivo models of neuronal diseases. Nevertheless, no study has aimed at finding the neuroactive constituent(s) of CR. In this study, we identified active compounds in a CR extract (CRE) using bioactivity-guided fractionation. We first compared the anti-oxidative and neuroprotective activities of four fractions and the CRE total extract. Only the ethyl acetate (EA) fraction revealed strong activity, and further isolation from the bioactive EA fraction yielded nine constituents: scirpusin A (1), scirpusin B (2), luteolin (3), 6'-acetyl-3,6-diferuloylsucrose (4), 4',6' diacetyl-3,6-diferuloylsucrose (5), p-coumaric acid (6), ferulic acid (7), pinellic acid (8), and fulgidic acid (9). The activities of constituents 1-9 were assessed in terms of anti-oxidative, neuroprotective, anti-inflammatory, and anti-amyloid-${\beta}$ activities. Constituents 1, 2, and 3 exhibited strong activities; constituents 1 and 2 were characterized for the first time in this study. These results provide evidence for the value of CRE as a source of multi-functional neuroprotectants, and constituents 1 and 2 may represent new candidates for further development in therapeutic use against neurodegenerative diseases.

Computational Fluid Dynamic Modeling for Internal Antenna Type Inductively Coupled Plasma Systems (CFD를 이용한 내장형 안테나 유도 결합 플라즈마 시스템 모델링)

  • Joo, Jung-Hoon
    • Journal of the Korean Vacuum Society
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    • v.18 no.3
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    • pp.164-175
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    • 2009
  • CFD is used to analyze gas flow characteristics, power absorption, electron temperature, electron density and chemical species profile of an internal antenna type inductively coupled plasma system. An optimized grid generation technology is used for a complex real-scale models for industry. A bare metal antenna shows concentrated power absorption around rf a feeding line. Skin depth of power absorption for a system is modeled to 50 mm, which is reported 53 mm by experiments. For an application of bipolar plates for hydrogen fuel cells, multi-sheet loading ICP nitriding system is proposed using an internal ICP antenna. It shows higher atomic nitrogen density than reported simple pulsed dc nitriding systems. Minimum gap between sheets for uniform nitriding is modeled to be 39 mm.