• Biotechnology and Bioprocess Engineering:BBE
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• v.11 no.4
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• pp.319-324
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• 2006

Although the sera used in animal cell culture media provide the macromolecules, nutrients, hormones, and growth factors necessary to support cell growth, it could also be an obstacle to the production of recombinant proteins in animal cell culture systems used in many sectors of the biotechnology industry. For this reason, many research groups, including our laboratory, have been trying to develop serum-free media (SFM) or serum-supplemented media (SSM) for special or multi-purpose cell lines. The Chinese hamster ovary (CHO) cell, for example, is frequently used to produce proteins and is especially valuable in the large-scale production of pharmaceutically important proteins, yet information about its genome is lacking. Also, SFMs have only been evaluated by comparing growth patterns for cells grown in SFMs with those grown in SSM or by measuring the titer of the target protein obtained from cells grown in each type of medium. These are not reliable methods of obtaining the type of information needed to determine whether an SFM should be replaced with an SSM. We carried out a cDNA microarray analysis to evaluate MED-3, an SFM developed in our laboratory, as a CHO culture medium When CHO cells were cultured in MED-3 instead of an SSM, several genes associated with cell growth were down-regulated, although this change diminished over time. We found that the insulin-like growth factor (IGF) gene was representative of the proteins that were down-regulated in cells cultured in MED-3. When several key supplements - including insulin, transferrin, ethanolamine, and selenium - were removed from MED-3, the IGF expression was consistently down- regulated and cell growth decreased proportionately. Based on these results, we concluded that when an SFM is used as a culture medium, it is important to supplement it with substances that can help the cells maintain a high level of IGF expression. The data presented in this study, therefore, might provide useful information for the design and development of SFM or SSM, as well as for the design of genome-based studies of CHO cells to determine how they can be used optimally for protein production in pharmaceutical and biomedical research.

• Korean Journal of Community Nutrition
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• v.10 no.5
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• pp.668-676
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• 2005

The purpose of this study was to determine the relationship between the students' levels of involvement in school lunch service and their satisfaction levels with the service. A survey was conducted with $5^{th}\;and\;6^{th}$ grade elementary students eating school lunches. Out of 1,680 questionnaires distributed to the students from 14 schools, 1,254 complete questionnaires $(74.6\%)$ were analyzed. The questionnaire included two 5-point multi-item scales for measuring levels of involvement in and satisfaction with school lunch service, respectively. A factor analysis grouped 20 items measuring school lunch satisfaction into three factors: 'food satisfaction', 'nutrition & sanitation satisfaction', and 'service & environment satisfaction'. As a result of multiple regression analyses controlling the influence of such variables as students' grades, gender, school location, years and places of eating school lunches, students' levels of involvement in school lunch service was proven to be positively associated with levels of school lunch satisfaction overall, as well as satisfaction levels in regard to 'food', 'nutrition & sanitation', and 'service & environment', respectively. It was suggested that efforts such as nutrition education to increase students' levels of involvement in school lunches could be a useful strategy to improve students' satisfaction with school lunch service.

• Journal of Energy Engineering
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• v.11 no.4
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• pp.332-341
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• 2002

This Paper discusses a theoretical approach to assess the acceptability on nuclear energy facili-ties and policies, that is associated with derivation of indicators influencing perception on the nuclear energy. Facets of the public perception include the necessity of nuclear energy, expected benefits and costs, possi-bility of control, nuclear energy risk sentiment level, and equality between present and future generations. It also identifies indicators directly or indirectly affecting the perception facets and classifies them into the knowledge-based and the trust-based. Knowledge on nuclear energy facility is acquired on the foundation of the understanding of fact, through information, education, PR, and experience the media. Meanwhile, trust on nuclear energy Policies as value judgment on reality is built through legitimacy, communication, compensa-tion, participation, and the media. Multi-dimensional analysis on nuclear energy acceptability will provide a key to developing a more realistic and mutually agreeable policies and solving the imminent issues.

• Health Policy and Management
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• v.27 no.4
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• pp.276-283
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• 2017

Background: Electronic cigarette (e-cigarette) has rapidly increased. Even though e-cigarettes are actually not smoking cessation alternative supplies, some people perceive them as smoking cessation alternative supplies. The purpose of this study is to identify the relationship between e-cigarette experiences and smoking cessation behaviors among Korean adults. Methods: Cross-sectional data was collected from 7,550 participants of Korea National Health and Nutrition Examination Survey in 2014. A total of 867 participants who were current smokers were enrolled in this study. Multi-variable logistic regression analysis were performed to identify the relationship between the smoking cessation behavior and the e-cigarette experience. Results: The e-cigarette experience rate of current smokers was 22.1%. The e-cigarette experience rate was higher in groups with the higher income, the higher education level, and the white collar jobs. Smokers with experience of smoking cessation (odds ratio [OR], 3.03; 95% confidence interval [CI], 1.67 to 5.76) and with intention to smoking cessation in the future (OR, 1.59; 95% CI, 1.05 to 2.43) were more likely to experience the e-cigarette. Especially, smokers who used smoking cessation alternative supplies were more likely to experience the e-cigarette than smokers who did not use it (OR, 2.87; 95% CI, 1.59 to 5.18). Conclusion: We confirmed that e-cigarette experiences were associated with smoking cessation experience, smoking cessation plan, and use of smoking cessation aid among smokers in Korea. Additional studies should be continued to confirm whether e-cigarette are continuously influencing the smoking cessation of current smokers in South Korea as one of the smoking cessation alternative supplies.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.4 no.1
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• pp.62-77
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• 2010

Emerging applications with high data rates will need to transport bulk data reliably in wireless sensor networks. ARQ (Automatic Repeat request) or Forward Error Correction (FEC) code schemes can be used to provide reliable transmission in a sensor network. However, the naive ARQ approach drops the whole frame, even though there is a bit error in the frame and the FEC at the bit level scheme may require a highly complex method to adjust the amount of FEC redundancy. We propose a bulk data transmission scheme based on erasure-resilient code in this paper to overcome these inefficiencies. The sender fragments bulk data into many small blocks, encodes the blocks with LT codes and packages several such blocks into a frame. The receiver only drops the corrupted blocks (compared to the entire frame) and the original data can be reconstructed if sufficient error-free blocks are received. An incidental benefit is that the frame error rate (FER) becomes irrelevant to frame size (error recovery). A frame can therefore be sufficiently large to provide high utilization of the wireless channel bandwidth without sacrificing the effectiveness of error recovery. The scheme has been implemented as a new data link layer in TinyOS, and evaluated through experiments in a testbed of Zigbex motes. Results show single hop transmission throughput can be improved by at least 20% under typical wireless channel conditions. It also reduces the transmission time of a reasonable range of size files by more than 30%, compared to a frame ARQ scheme. The total number of bytes sent by all nodes in the multi-hop communication is reduced by more than 60% compared to the frame ARQ scheme.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.41 no.11
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• pp.921-930
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• 2013

Satrec Initiative successfully developed and verified a high-resolution electro-optical camera system, EOS-D Ver.1.0. We designed this system to give improved spatial and radiometric resolution compared with EOS-C series systems. The thermal control subsystem (TCS) of the EOS-D Ver.1.0 uses heaters to meet the opto-mechanical requirements during in-orbit operation and uses different thermal coatings and multi-layer insulation (MLI) blankets to minimize the heater power consumption. Also, we designed and verified a refocusing mechanism to compensate the misalignment caused by moisture desorption from the metering structure. We verified the design margin and workmanship by conducting the qualification level thermal vacuum test. We also performed the verification of thermal math model (TMM) by comparing with thermal balance test results. As a result, we concluded that it faithfully represents the thermal characteristics of the EOS-D Ver.1.0.

• Food Science of Animal Resources
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• v.38 no.2
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• pp.391-402
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• 2018

The aim of the present work was to provide information about Enterococcus strains isolated from pre-packaged chicken samples in Ankara (Turkey), focusing on their prevalence, phenotypic and genotypic characteristics, and antibiotic resistance. We report the first study on the occurrence of antibiotic resistant enterococci in pre-packaged chicken samples in Ankara. A total of 97 suspicious enterococcal isolates were identified from 122 chicken samples. All isolates were identified to species level by phenotypic and molecular methods. In the 16S rDNA sequence analysis, Enterococcus faecium (61.85%) and Enterococcus faecalis (38.15%) were found to be the most frequently detected Enterococcus spp. Of the 97 isolates tested for hemolytic activity, 12.37% enterococcal strains were ${\beta}$-hemolytic. ${\beta}$-Hemolysin was most prevalent among E. faecium (58.33%) compared to E. faecalis (41.66%). Disk diffusion method was used for determining of antibiotic resistance. The analysis of the antimicrobial resistance of the 97 Enterococcus isolates revealed that the resistance to kanamycin (98.96%), rifampicin (80.41%) and ampicillin (60.82%) was most frequent. Furthermore, resistance to erythromycin (38.14%) and ciprofloxacin (34.02%) was also observed. The frequencies of resistance to tetracycline (9.27%), penicillin G (8.24%), and chloramphenicol (3.09%), gentamicin (2.06%) and streptomycin (1.03%) were low. None of the isolates was resistant to vancomycin. Multi-drug resistance was found in 97.93% of Enterococcus strains. E. faecium strains showed a more resistant phenotype than E. faecalis strains according to the antibiotic resistance levels. The results of this study indicated that chicken meat is a potential reservoir for the transmission of antibiotic resistance from animals to humans.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.27 no.1
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• pp.713-722
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• 2009

Gravity measurement can determine the earth gravitational field, also is the fundamental to the research of earth gravitational field, geodesy and geodynamic, vertical movement of the crust, geoid surface, sea level and climate etc. Recently, National Geographic Information Institute (NGII) introduced FG-5 absolute gravity meter in order to lay a foundation for establishment of Absolute Gravity Network, and furthermore NGII plan to construct about 1,200 multi dimensional and function Unified Control Points(UCP) in nationwide. It will play an important role in development of high accuracy geoid model in South Korea. This paper explains the fundamental theory and method of relative gravity measurement, surveys the relative gravity of 21 stations using latest Scintrex CG-5 relative gravimeter. In addition, it calculates gravity values, compare and analysis gravity survey results using datum-free adjustment and weighted constraint adjustment. The results indicate show that datum-free and weighted constraint adjustment methods are available to determine high accuracy gravity achievement, datum-free method is more advantage than weighted constraint adjustment.

• Journal of Korean Society for Atmospheric Environment
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• v.28 no.4
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• pp.384-395
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• 2012

This study was performed to develop the source profiles for fine particles ($PM_{2.5}$) emitted from the biomass burning. The multi-method research strategy included a usage of combustion devices such as field burning, fireplace, and residential wood burning to burn rice straw, fallen leaves, pine tree, and oak tree. The data were collected from multiple sources and measured water-soluble ions, elements, elemental carbon (EC), and organic carbon (OC). From this study, it turned out that OC (34~67%) and EC (1.2~39%) are the major components emitted from biomass burning. In the case of burning rice straw at field burning, OC (66.6%) was the most abundant species, followed by EC (4.3%), $Cl^-$ (3.6%), Cl (2.1%), and $SO^{2-}_4$(1.9%). Burning rice straw, fallen leaves, pine tree, and oak tree at fireplace, the amount of OC was 58.5%, 52.7%, 52.5%, and 61.2%, and that of EC was 1.2%, 18.4%, 36.5%, and 2.7%, respectively. The ratio of OC for the burning of pine tree and oak tree from the residential wood burning device was 56.9% and 34.3%, and that of EC was 25% and 38.6%, respectively. Applying the measured data with respect to the proportion of components emitted from biomass burning to reference model, it turned out that self-diagnosed result was appropriate level, and the result based on the model is in highly corresponding to actual timing of biomass burning.

• Proceedings of the Korean Society for Bioinformatics Conference
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• 2002.06a
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• pp.123-137
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• 2002

생물정보공학을 위한 학문적/실용적 접근은 전산학, 생물학, 유전공학, 수학/통계학등이 유기적으로 통합되어 이루어져야 한다. 그러나 각계의 전문가가 서로의 특정 지식을 활용하기 위한 물리적인 기반이 갖추어져 있지 않은 상태에서는 각 분야의 전문적 지식 활용이 용이하지 않다. 현재의 의료 서비스 제공자/병원이 가진 방대한 의료 데이터를 생물정보공학 령역에서 활용할 수 있도록 해야 하고, 진료 데이터에 근거한 유전적 정보 분석을 위해 생물학 전문가들이 생성하는 인간 질병에 관한 유전적 분석, 연구 결과를 다시 의료 서비스 제공자에게 돌려주는 순환적 사이클이 필요하고, 이러한 순환적 사이클 지원자는 정보 기술이라고 생각한다. 인간 질병 극복과 좀 더 나은 진료, 예방책을 제공할 수 있도록 생물정보공학, 의료정보학, 컴퓨터과학의 통합 활용 목표를 설정할 수 있다. 각계의 전문가가 지식을 공유할 수 있고 기존의 병원 시스템 및 유전 연구소 등의 시스템을 통합하여 유기적으로 엮음으로써 데이터를 의미 있게 해석하고 공유할 수 있도록 지원하는 프레임워크가 절실히 요구된다. 본 세미나에서는 의료정보학과 생물정보공학에서 활용하는 시스템 통합, 전문 지식의 통합적 활용을 위해 각 전문가를 대신하는 에이전트로 구성된 멀티에이전트 플랫폼을 제시하여, 각 분야가 갖는 전문성 확보, 광고, 유기적 연결을 멀티에이전트 시스템에게 위임함으로써 각 영역에서 서비스 할 수 있는 내용과 서비스 제공 주체인 각계의 전문가 집단을 유기적으로 통합하고자 한다. 의료 영역에서 이루어진 의료 영상 통신 시스템 (Picture Archiving and Communication Systems), 의료 정보 표준화를 위한 HL7 (Health Level 7)에 대해서 경북대학교 지능정보 연구실에서 연구, 개발한 내용을 발표한다. 의료 정보 시스템과 생물학 영역의 유전체 정보 데이터베이스 시스템 사이에 의미 있는 데이터 전송, 지식 획득을 위해 정보 기술 분야에서 활용해야 할 영역으로 XML Web Services, Multi-agent Systems, 전문가 컴뮤니티를 위한 그룹웨어 연구 개발에 관해 사례 중심으로 발표한다.