• 한국환경보건학회지
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• 제47권1호
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• pp.64-77
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• 2021

Objective: Biological monitoring of trace elements in human blood samples has become an important indicator of the health environment. The purpose of this study was to detect and evaluate multiple metal items in blood samples based on ICP-MS, to perform comparative evaluation with the existing analysis method, and to develop and verify a new method. Methods: 100 μL of whole blood from 80 healthy subjects was used to analyze ten metals (Sb, tAs, Cd, Pb, Mn, Hg, Mo, Ni, Se, Tl) using ICP-MS. Verification of the analysis method included calculation of linearity, accuracy, precision and detection limits. In addition, a comparative test with the conventional graphite furnace atomic absorption spectroscopy (GF-AAS) method was performed. In the case of Pb, Cd, and Hg in whole blood, cross-analysis between Pb, Cd, and Hg analysis methods was performed to confirm the difference between the existing method and the new method (ICP-MS). Results: The coefficient of determination (R2) was 0.999 or higher in seven items and 0.995 or higher in three items. The Pb result showed that Pearson's correlation coefficient was very high at 0.983, and the intraclass correlation coefficient was 0.966. The Cd result showed that Pearson's correlation coefficient was 0.917 between the existing method and the new analysis concentration value. Its intraclass correlation coefficient was 0.960, and there was no significant difference between the two groups. Hg had a low correlation at 0.687, and the intraclass correlation coefficient was 0.761, which was lower than that of Pb and Cd. The intra-day and inter-day accuracy of Pd and Cd were satisfactory, but Hg did not meet the criteria for both accuracy and precision when compared with the conventional analysis method. Conclusion: This study can be meaningful in that it proposes a more efficient and feasible analysis method by verifying a blood heavy metal concentration experiment using multiple simultaneous analyses. All samples were processed and analyzed using the new ICP-MS. It was confirmed that the agreement between the two methods was very high, with the agreement between the current and new methods being 0.769 to 0.998. This study proposes an efficient simultaneous methodology capable of analyzing multiple elements with small samples. In the future, studies of various applications and the reliability of ICP-MS analysis methods are required, and research on the verification of accurate, precise, and continuous analysis methods is required.

• Journal of Applied Biological Chemistry
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• 제63권4호
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• pp.347-355
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• 2020

기상이변으로 인한 식물 스트레스를 평가하기 위해 토양과 식물에 적용할 수 있는 여러 센서가 개발되었다. 따라서 본 연구의 목적은 식물의 온도 스트레스를 평가하기 위해 다양한 센서를 사용하여 기후 및 토양 상태와 식물 반응을 모니터링하는 것이다. 식물 반응을 평가하기 위한 식물 센서로 식물 줄기의 전기전도도, sap flow rate를 모니터링하였다. 식물 줄기의 전기전도도는 물과 이온 수송을 포함한 식물의 생리적 활성을 반영한다. 완전히 자란 Brassica oleracea var. italica를 온도 20/15 ℃ (낮/밤), 16시간 광주기로 하여 대조구로 설정하였으며 저온 15/10 ℃ 및 고온 35/30 ℃에 노출시키면서 토양 및 식물 상태를 모니터링하였다. 식물 줄기의 전기전도도, sap flow rate는 낮에는 증가하고 밤에는 감소하였다. 저온 스트레스 하에서 Brassica oleracea var. italica 줄기의 전기전도도는 대조구보다 낮았고, 고온 스트레스 하에서는 대조구보다 높아 물과 이온 수송이 온도에 의해 영향을 받았음을 나타낸다. 그러나 엽록소 a와 b 함량은 저온 스트레스를 받은 잎에서 증가했고 고온 스트레스를 받은 잎에서는 대조구와 차이가 없었다. 잎의 프롤린 함량은 저온 스트레스에서는 대조구와 차이가 없는 반면, 고온 스트레스에서는 증가했다. 프롤린 합성은 환경 스트레스 하에서 식물의 방어 메커니즘으로 작용한다. 따라서 Brassica oleracea var. Italica는 저온보다 고온 스트레스에 더 민감한 것으로 판단된다.

• 한국시뮬레이션학회논문지
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• 제29권4호
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• pp.9-19
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• 2020

스마트폰의 빠른 보급과 함께 소셜네트워크서비스(Social Network Service, SNS)의 이용자 수는 매우 빠르게 증가하고 있다. 2018년 기준 전 세계 인구 약 71억명 중 27억명이 SNS를 사용하는 것으로 나타났으며, 대한민국에서는 약 5,100만명 중 약 3,120만 명이 SNS를 사용하는 것으로 나타났다. 이와 같이 지속적으로 성장해온 SNS 시장에 대해 다양한 분석연구가 존재해왔으나, 수리적 모델을 통한 정량적 분석 연구는 미진한 실정이다. 본 연구에서는 국내 SNS 시장에서 가장 큰 비중을 차지하고 있는 페이스북, 인스타그램, 트위터를 대상으로 경쟁 관계 분석을 수행하였다. 본 연구의 목적은 SNS 시장의 흐름과 경쟁관계에 관한 몇 가설을 제시하고 해당 가설을 검정하여 SNS 시장의 경향성을 분석하는 것이다. 경쟁관계분석을 위해 Lotka-Volterra(LV) 모형을 적용하였으며, 시간의 흐름에 따른 경쟁관계 변화를 파악하기 위해 Moving Window 기법을 함께 활용하였다. 제시한 가설을 검정 하기위해 Moving Window 기법을 활용하여 몇 가지 시뮬레이션분석을 진행하였다. 각 플랫폼 간의 상관관계와 시간의 흐름에 따른 경쟁관계의 변화를 확인하였다.

• 동의신경정신과학회지
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• 제32권2호
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• pp.81-93
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• 2021

Objectives: Mild cognitive impairment (MCI) is condition of cognitive decline shown in transition from normal aging to dementia. Hominis placenta pharmacopuncture (HPP) is a treatment that combines effects of medication and acupuncture by injecting Hominis placenta into acupoints. The objective of this study was to evaluate the efficacy and safety of HPP for MCI. Methods: This was a randomized, double-blind, placebo-controlled, two-center clinical trial. Eligible patients were randomly allocated to either the HPP group or the placebo group. HPP or saline as placebo was administered to participants for eight weeks. Changes in symptoms were observed. The primary outcome was difference in mean change of Korean Version of the Montreal Cognitive Assessment (MoCA-K) score between the HPP group and the placebo group. Cognitive function, overall status of mood and sleep, and quality of life (QoL) were also assessed. Safety assessment and economic analysis were then conducted. Results: Thirty participants were enrolled. One participant in the placebo group dropped out. The score of MoCA-K increased after treatment. Its mean change was smaller in the HPP group than in the control group. HPP ameliorated Global Deterioration Scale and Korean Dementia Rating Scale subtests for attention, organization, and memory compared to the placebo. However, none of them was significantly different between the two groups. Mood, sleep, and QoL all improved more in the HPP group than in the placebo group, although differences between the two groups were not statistically significant. There was no adverse event probably related to the drug. HPP treatment needed KRW 345,000 more than the placebo group in improving Geriatric Quality of Life scale-Dementia score by one point for one year. Conclusions: Although HPP treatment did not significantly improve cognition, it changed behavioral and psychological symptoms in MCI.

• 대한치과교정학회지
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• 제52권1호
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• pp.66-74
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• 2022

Objective: To investigate demographic and skeletodental characteristics of one-jaw (1J-OGS) and two-jaw orthognathic surgery (2J-OGS) in patients with skeletal Class III malocclusion. Methods: 750 skeletal Class III patients who underwent OGS at 10 university hospitals in Korea between 2015 and 2019 were investigated; after dividing them into the 1J-OGS (n = 186) and 2J-OGS groups (n = 564), demographic and skeletodental characteristics were statistically analyzed. Results: 2J-OGS was more frequently performed than 1J-OGS (75.2 vs. 24.8%), despite regional differences (capital area vs. provinces, 86.6 vs. 30.7%, p < 0.001). Males outnumbered females, and their mean operation age was older in both groups. Regarding dental patterns, the most frequent maxillary arch length discrepancy (ALD) was crowding in the 1J-OGS group (52.7%, p < 0.001) and spacing in the 2J-OGS group (40.4%, p < 0.001). However, the distribution of skeletal pattern was not significantly different between the two groups (all p > 0.05). The most prevalent skeletal patterns in both groups were hyper-divergent pattern (50.0 and 54.4%, respectively) and left-side chin point deviation (both 49.5%). Maxillary spacing (odds ratio [OR], 3.645; p < 0.001) increased the probability of 2J-OGS, while maxillary crowding (OR, 0.672; p < 0.05) and normo-divergent pattern (OR, 0.615; p < 0.05) decreased the probability of 2J-OGS. Conclusions: In both groups, males outnumbered females, and their mean operation age was older. The most frequent ALD was crowding in the 1J-OGS group, and spacing in the 2J-OGS group, while skeletal characteristics were not significantly different between the two groups.

• Animal Bioscience
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• 제35권11호
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• pp.1787-1799
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• 2022

Objective: Choline deficiency, one main trigger for nonalcoholic fatty liver disease (NAFLD), is closely related to lipid metabolism disorder. Previous study in a choline-deficient model has largely focused on gene expression rather than gene structure, especially sparse are studies regarding to alternative splicing (AS). In modern life science research, primary hepatocytes culture technology facilitates such studies, which can accurately imitate liver activity in vitro and show unique superiority. Whereas limitations to traditional hepatocytes culture technology exist in terms of efficiency and operability. This study pursued an optimization culture method for duck primary hepatocytes to explore AS in choline-deficient model. Methods: We performed an optimization culture method for duck primary hepatocytes with multi-step digestion procedure from Pekin duck embryos. Subsequently a NAFLD model was constructed with choline-free medium. RNA-seq and further analysis by rMATS were performed to identify AS events alterations in choline-deficency duck primary hepatocytes. Results: The results showed E13 (embryonic day 13) to E15 is suitable to obtain hepatocytes, and the viability reached over 95% by trypan blue exclusion assay. Primary hepatocyte retained their biological function as well identified by Periodic Acid-Schiff staining method and Glucose-6-phosphate dehydrogenase activity assay, respectively. Meanwhile, genes of alb and afp and specific protein of albumin were detected to verify cultured hepatocytes. Immunofluorescence was used to evaluate purity of hepatocytes, presenting up to 90%. On this base, choline-deficient model was constructed and displayed significantly increase of intracellular triglyceride and cholesterol as reported previously. Intriguingly, our data suggested that AS events in choline-deficient model were implicated in pivotal biological processes as an aberrant transcriptional regulator, of which 16 genes were involved in lipid metabolism and highly enriched in glycerophospholipid metabolism. Conclusion: An effective and rapid protocol for obtaining duck primary hepatocytes was established, by which our findings manifested choline deficiency could induce the accumulation of lipid and result in aberrant AS events in hepatocytes, providing a novel insight into various AS in the metabolism role of choline.

• 대중서사연구
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• 제26권4호
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• pp.117-144
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• 2020

영화 <버닝>(이창동, 2018)은 정교한 서사를 섬세한 영상으로 담아낸 작품으로서 근래 가장 주목받은 한국영화 중 한 편이다. 이 영화는 서로 다른 유형의 인물들이 등장하고 단편적으로 제시되는 객관적 사실과 주관적 욕망이 뒤얽히는 다성성의 텍스트여서 다양한 해석의 여지가 있는 텍스트이다. 이 글은 이 영화가 현대 한국 청년의 현실이라는 특정한 사회역사적 조건을 넘어서는 보편적이면서도 중대한 윤리의 문제를 제기하고 있는 점에 주목해 <버닝>을 주인공 종수를 중심으로 하는 윤리적 담론으로 읽으려 한다. 종수가 처하는 상황과 이에 대한 그의 반응을 무엇보다도 종수의 윤리적 각성과 도약의 관점에서 살펴보고자 한다. 종수는 현재를 사는 대한민국 비정규직 젊은 남성이라고 요약될 수 있는 자신의 사회적 위치에서 해미와 벤을 만나고 관계 맺으며 세상의 미스터리를 접하고 이를 파악하고자 한다. 영화가 촘촘히 보여주는 그의 궤적은 문예창작학과 출신 흙수저 청년의 혼란과 좌절이라는 사회역사적인 차원과 가족해체의 현실과 연인의 갑작스런 실종이라는 개인 심리적 차원이 필연적으로 교차한다. <버닝>은 종수가 비우호적인 조건, 즉 프레카리아트 청년의 처지, 현실 인식의 불확실성에도 불구하고, 세상을 직면하고 미스터리를 탐구하면서 '함께 삶'을 지향하는 윤리적 주체로 우뚝 서는 과정을 그린 감동적인 스토리로 읽힌다. 이 과정은 종수의 고통스런 성장담으로서 무기력했던 비정규직 배달노동자였던 종수가 '작가'가 되는 과정이기도 하다. <버닝>은 자신에게 비우호적인 현실 속에서 혼란스러워 하고 무기력했던 청년이 타자들과의 만남을 겪은 뒤 나름대로 타자들과 세상을 파악하며 자신을 돌아보고 자신이 해야 할 일을 실천하는 윤리적 주체로 일어서는 과정을 그리며, 윤리적 사유를 자극하는 텍스트이다.

• Animal Bioscience
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• 제36권1호
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• pp.29-42
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• 2023

Objective: Pigs, an ideal biomedical model for human diseases, suffer from about 50% early embryonic and fetal death, a major cause of fertility loss worldwide. However, identifying the causal variant remains a huge challenge. This study aimed to detect single nucleotide polymorphisms (SNPs) and candidate genes for the number of mummified (NM) piglets using the imputed whole-genome sequence (WGS) and validate the potential candidate genes. Methods: The imputed WGS was introduced from genotyping-by-sequencing (GBS) using a multi-breed reference population. We performed genome-wide association studies (GWAS) for NM piglets at birth from a Landrace pig populatiGWAS peak located on SSC11: 0.10 to 7.11 Mbp (Top SNP, SSC11:1,889,658 bp; p = 9.98E-13) was identified in cyclin dependent kinase on. A total of 300 Landrace pigs were genotyped by GBS. The whole-genome variants were imputed, and 4,252,858 SNPs were obtained. Various molecular experiments were conducted to determine how the genes affected NM in pigs. Results: A strong GWAS peak located on SSC11: 0.10 to 7.11 Mbp (Top SNP, SSC11:1,889,658 bp; p = 9.98E-13) was identified in cyclin dependent kinase 8 (CDK8) gene, which plays a crucial role in embryonic retardation and lethality. Based on the molecular experiments, we found that Y-box binding protein 1 (YBX1) was a crucial transcription factor for CDK8, which mediated the effect of CDK8 in the proliferation of porcine ovarian granulosa cells via transforming growth factor beta/small mother against decapentaplegic signaling pathway, and, as a consequence, affected embryo quality, indicating that this pathway may be contributing to mummified fetal in pigs. Conclusion: A powerful imputation-based association study was performed to identify genes associated with NM in pigs. CDK8 was suggested as a functional gene for the proliferation of porcine ovarian granulosa cells, but further studies are required to determine causative mutations and the effect of loci on NM in pigs.

• Animal Bioscience
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• 제36권1호
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• pp.10-18
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• 2023

Objective: In this study, we aimed to position the Hungarian Merino among other Merinoderived sheep breeds, explore the characteristics of our sampled animals' genetic similarity network within the breed, and highlight single nucleotide polymorphisms (SNPs) associated with daily weight-gain. Methods: Hungarian Merino (n = 138) was genotyped on Ovine SNP50 Bead Chip (Illumina, San Diego, CA, USA) and positioned among 30 Merino and Merino-derived breeds (n = 555). Population characteristics were obtained via PLINK, SVS, Admixture, and Treemix software, within-breed network was analysed with python networkx 2.3 library. Daily weight gain of Hungarian Merino was standardised to 60 days and was collected from the database of the Association of Hungarian Sheep and Goat Breeders. For the identification of loci associated with daily weight gain, a multi-locus mixed-model was used. Results: Supporting the breed's written history, the closest breeds to Hungarian Merino were Estremadura and Rambouillet (pairwise F_ST values are 0.035 and 0.036, respectively). Among Hungarian Merino, a highly centralised connectedness has been revealed by network analysis of pairwise values of identity-by-state, where the animal in the central node had a betweenness centrality value equal to 0.936. Probing of daily weight gain against the SNP data of Hungarian Merinos revealed five associated loci. Two of them, OAR8_17854216.1 and s42441.1 on chromosome 8 and 9 (-log₁₀P>22, false discovery rate<5.5e-20) and one locus on chromosome 20, s28948.1 (-log₁₀P = 13.46, false discovery rate = 4.1e-11), were close to the markers reported in other breeds concerning daily weight gain, six-month weight, and post-weaning gain. Conclusion: The position of Hungarian Merino among other Merino breeds has been determined. We have described the similarity network of the individuals to be applied in breeding practices and highlighted several markers useful for elevating the daily weight gain of Hungarian Merino.

• 한국수자원학회논문집
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• 제55권11호
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• pp.887-901
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• 2022

개별 강우-유출 사상을 대상으로 최적 매개변수를 산정하는 경우 사상별로 매개변수가 서로 다르며 물리적인 범위를 중심으로 변동성이 커 유역의 대표 매개변수를 결정하는데 어려움이 있다. 매개변수 추정 시 변동성 증가는 강우의 시공간적 변동성과 함께, 유역 내 일부 홍수량 산정지점 기준으로 강우-유출 자료만이 이용 가능하여 매개변수의 식별성(identifiability)이 매우 낮다. 추정되는 매개변수의 변동성 확대에 따른 문제점을 개선하기 위하여, 본 연구에서는 다수의 사상들을 동시에 고려한 매개변수 최적화 방법을 제안하였으며, NSE를 목적함수로 하여 매개변수를 최적화하였다. 개별 사상들을 통합적으로 고려하여 최적매개변수를 산정하는 경우 매개변수의 물리적인 특성을 유지함과 동시에 유역의 공동 매개변수 효율적으로 추정이 가능하였다. 개별 매개변수와 공통 매개변수 NSE의 차이가 최대 0.08 정도를 나타내며, 홍수량 재현 측면에서도 개별적으로 최적화를 수행한 경우와 유사하거나 보다 개선된 결과를 확인할 수 있었다.