• International Journal of Industrial Entomology and Biomaterials
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• v.40 no.2
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• pp.41-50
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• 2020

Vermicompost, manure, compost and organic fertilization are ecofriendly. Nowadays, many products resulted from sericulture consumed by humans such as mulberry leaves, fruits, mulberry tea, silk and natural silk cosmetics. Soil applications of three treatments with vermicompost (0.5, 1 and 2 tons per 0.42 hectare) and recommended rate of mineral fertilizers of nitrogen phosphorus potassium were used for investigation. Impact of fertilization on mulberry plant traits of moisture, number of shoots/tree, total shoots length/tree, number of leaves/shoot, number of leaves/ (100g), leaf yield/tree and leaf yield of fadden/season were recorded. In addition the effect of fertilization on larval and cocoon characters of young instar duration, fifth instar duration, total larval duration, larval mortality percentage, weight of third instar larvae, weight of fourth instar larvae, weight of fifth instar larvae, fresh cocoon weight, fresh shell weight, pupae weight, cocoon shell ratio, silk productivity, cocooning percentage, pupation ratio, number of cocoons/ liter, crop cocoons by number, crop cocoons by weight, fecundity and fertility. Using vermicompost treatment was enhancing plant characters. Treatments of V₃, V₂ and V₁ were shortage young, fifth and larvae durations. Mostly feeding silkworm during the whole larval duration on treated mulberry leaves with vermicompost improving the traits average. Using vermicompost for fertilization by rate of V₃ and V₂ is better than others for cocoon characters for females and males.V₃ and V₂ of vermicompost per 0.42 hectare is recommended for rearing mulberry silkworm instead of mineral fertilization.

• Journal of Sericultural and Entomological Science
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• v.9
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• pp.21-25
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• 1969

Various formulae for estimation of leaf production in mulberry trees were investigated and obtained. Four varieties of mulberry trees were used as the materials, and four characters. namely branch length (X, 1). branch diameter (X, 2). leaf number per branch (X, 3), and leaf area per branch (X, 4). were studies. The formulae to eatimate the leaf yield of mulberry trees are as follows: 1. Y$_1$v$_1$＝-115.760＋0.068X$_1$＋165.756X$_2$ Y$_1$v$_2$＝-221.500＋1.768X$_1$＋38.152X$_2$ Y$_1$v$_3$＝-253.826-0.116X$_1$＋289.507X$_2$ Y$_1$v$_4$＝ -157.559＋1.063X$_1$＋106.088X$_2$ where Y$_1$v$_1$, Y$_1$v$_2$, Y$_1$v$_3$, Y$_1$v$_4$, are showed the estimated yield of the each variety, namely Gaeryang souban, Ilchirye, Nosang. and Suwon Sang No. 4, respectively. X$_1$ and X$_2$ denote the measured values of branch length and branch diameter, respectively. 2. Y$\sub$7/v$_1$＝-118.478-0.665X$_1$＋184.445X$_2$＋2.346X$_3$ Y$\sub$7/v$_2$＝-217.432＋2.062X$_1$＋35.668X$_2$-1.058X$_3$ Y$\sub$7/v$_3$＝-206. 249-0.739X$_1$＋268.08X$_2$＋2.770X$_3$ Y$\sub$7/v$_4$＝-153.383＋0.009X$_1$＋2.024X$_2$＋0.171X$_3$where Y$\sub$7/v$_1$, Y$\sub$7/v$_2$, Y$\sub$7/v$_3$, Y$\sub$7/v$_4$, are the estimated yield of the each variety, namely Gaeryang. Souban, Ilichirye, Nosang, and Suwon Sang No. 4, respectively. X$_1$, X$_2$, X$_3$, denote the measured values of each character. branch length, branch diameter and leaf number per branch, respectively. 3. Y$\sub$11/v$_1$＝82. 567-1.283X$_1$＋15.501X$_2$＋0.640X$_3$＋3.511X$_4$ Y$\sub$11/v$_2$＝136.411＋0.311X$_1$＋1.921X$_2$-0. 217X$_3$＋0.214X$_4$ Y$\sub$11/v$_3$＝150.2Z7-0.139X$_1$＋11.788X$_2$＋0.143X$_3$＋0.381X$_4$ Y$\sub$11/v$_4$＝160.850＋0.323X$_1$＋66.076X$_2$-0.794X$_3$＋2..614X$_4$ where Y$\sub$11/v$_1$, Y$\sub$11/v$_2$, Y$\sub$11/v$_3$, Y$\sub$11/v$_4$, are the estimated yield values of four varieties, and X$_1$, X$_2$, X$_3$, X$_4$ denote the measured values of four characters. namely branch length, branch diameter. leaf number per branch and leaf area per branch. respectively. The estimation method of mulberry leaf yield by measurement of some characters, branch length. branch diameter. leaf number per branch and leaf area per branch. could be the better method to determine the leaf yield of mulberry trees without destroying the leaves and without weighting the leaves of mulberry trees than the other methods.

• International Journal of Industrial Entomology and Biomaterials
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• v.8 no.2
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• pp.175-179
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• 2004

Trichoderma harzianum-THN1 parasitising the egg masses of root knot nematode Meloidogyne incognita was isolated from galled mulberry roots and evaluated for its potential to control root knot disease. In pot experiments root galling was reduced and leaf yield increased significantly following soil treatment with T. harzianum-THN1. The extracts obtained from the soils inoculated with T. harzianum-THN1 drastically inhibited the hatching of nematode eggs and the effect was irreversible even after the eggs were transferred to fresh water. The fungus was equally effective in controlling the disease in nematode infested mulberry garden under field conditions which was significant over the most commonly used egg parasitic fungus Paecilomyces lilacinus. The disease reduction recorded with T. harzianum was on par with the plants treated with the nematicide Carbofuran. The results suggest that T. harzianum- THN1 could be used as a potent ecofriendly biocontrol agent against M. incognita in mulberry without any residual toxicity to silkworms. T. harzianum- THN1 can form an important component of integrated disease management package in mulberry cultivation.

• International Journal of Industrial Entomology and Biomaterials
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• v.48 no.2
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• pp.78-85
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• 2024

Bud sport (bud mutation) is a valuable source for existing new genotypes in mulberry (Morus spp.) as well as critical materials for studying the molecular mechanisms underlying essential traits. Thus, identification, collection, characterization, and conservation of such natural variants are prerequisites for enhancing the mulberry genetic resource in the germplasm. In this context, we identified and characterized three bud sports (VBS-1, VBS-2, and VBS-3) of a popular mulberry cultivar, Victory-1 (V-1). These bud sports are morphologically, anatomically, and genetically more distinct from their mother plant, Victory-1. Moreover, these bud sports display lower growth and yield potential. Furthermore, these showed remarkably lower 2C DNA contents of 0.74 pg (VBS-1), 0.78 pg (VBS-2), and 0.76 pg (VBS-3), when compared to their mother plant V-1 (2C = 0.81 pg). On the other hand, molecular characterization between the bud sports and their mother plant revealed the existence of genetic variation due to the natural bud mutation that occurred in the mulberry cultivar Victory-1.

• International Journal of Industrial Entomology and Biomaterials
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• v.16 no.2
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• pp.67-74
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• 2008

A line x tester analysis was carried out in mulberry (Morns spp.) under different salinity levels to determine the changes in the genetic interaction of various morpho-biochemical characters. Five mulberry genotypes, 3 females and 2 males, differing in salt tolerance were selected for the study. Clones of these parents along with clones of the F1 hybrids were planted in earthen pots and subjected to different levels of salinity (0.0%, 0.25%, 0.50%, 0.75% and 1.00% NaCl). Data on morphological and biochemical characters were subjected to line x tester analysis. The result revealed significant variation among the parents studied. The prominence of non-additive gene effect under control condition suggests the need for well chalked out breeding program to exploit the non-fixable variance of components for improvement of plant height, leaf size and leaf yield, chlorophyll and photosynthesis in mulberry. However, under salinity stress a shift from non-additive gene effect to additive gene effect for the above said character further suggests the need for a change in breeding strategy. The general combining ability (GCA) analysis has identified English black as the best combiner among the parents and the specific combining ability analysis (SCA) found crosses of English black X C776 and Rotndiloba x Mandalaya were good for Plant height and leaf size and English black X C776 and Rotundiloba x C776 were good for biochemical proline and chlorophyll. From the performance of parents and their crosses under different salinity levels and also under normal cultural conditions it is concluded that in mulberry different approaches are required to develop varieties for the irrigated and saline conditions.

• Journal of Sericultural and Entomological Science
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• no.12
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• pp.1-4
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• 1970

The experimental studies were intended to clarify the effects of leaf yield calculations, and also aimed at estimating the correlations between some characters for the selection of desirable stocks of mulberry trees. The analysis of covariances was the variance components procedures in a replicated trial for the varieties, and also it was used to obtain the genotypic, phenotypic and environmental correlations between the eight characters-branch length, node number, branch diameter, branch number per stock, total branch weight, old branch weight, new shoot and leaf weight, and leaf weight, etc. The results obtained are summarized as follows: Phenotypic, genotypic and environmental covariances are shown in Table 1 and phenotypic correlations, genotypic correlations and environmental correlations are shown in Table 2. Genotypic correlations were slightly higher than the corresponding phenotypic correlations between the characters in the varieties, but the variation in values due to the change of environment appeared between the characters. Genotypic correlations between the leaf yield of mulberry trees and other characters indicated that high leaf yield was genetically correlated with six characters namely branch length, node number, branch number per stock, total branch weight, old branch weight and new shoot weight, etc. It was also observed that lower correlations were appeared between the leaf yield and branch diameter, and branch diameter and other all characters studied in both genotypic and phenotypic correlation coefficients.

• International Journal of Industrial Entomology and Biomaterials
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• v.10 no.1
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• pp.65-68
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• 2005

Growth and development of a plant over a growing season is closely related to the daily accumulation of heat. Heat unit measured by accumulated growing degree days (GDD) is becoming increasingly popular to estimate the growth of a plant or even in insect. GDD or heat accumulation per day is measured by calculating average daily temperature and then subtracting the base temperature below which growth does not occur. Heat accumulation per day is added for the desired period and accumulated GDD is determined. The present study was conducted in five seasons in an established garden with K-2, S-36 and V-1 mulberry varieties belonging to Morus indica L. grown under completely irrigated condition at the farm of CSRTI, Mysore during 2001 - 2002. Plants were pruned in each season and the growth of the plant measured by total shoot length and fresh leaf yield was recorded at an interval of 5 days starting from 30 days of pruning (DAP) to 70 days when all the plants were pruned. The accumulated GDD for the corresponding days were recorded and used for analysis. Accumulated growing degree days (GDD) have been found to be perfectly correlated with both growth and yield in all the seasons in all the varieties studied. The high $R^2$ values indicated a strong relationship between the accumulated GDD and, growth and yield of mulberry.

• Journal of Sericultural and Entomological Science
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• v.44 no.1
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• pp.22-27
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• 2002

The Yongcheonppong was cultured in the form of a dense mulberry field coverd with polyethylene film and was tested in Suwon and Chunchon. The agronomic characteristic and some consideration on the result of the silkworm rearing were as follows. The Yongcheonppong was the medium budding variety and was longer in length of young shoots than the Kaeryangppong. The Yongcheonppong was higher in leaf yield in spring than the Kaeryangppong and was lower in leaf yield in autumn than the Kaeryangppong to the contrary. The Yongcheonppong was a little higher in the number of branches and was longer in the length of the branch than the Kaeryangppong and so was appropriate for rearing with mulberry shoots. Yongcheonppong was more resistant to twig blight and die-back and was a little susceptible to dwarf disease. As the Yongcheonppong was most resistant to the freezing injury, it was appropriate for the cold region and enabled the farmers to rear some amount of silkworms every year. In Chunchon, The Yongcheonppong increased by 11% in cocoon yield than the Kaeryangppong when the freezing injury occurred 1 year out of 2 years and produced about the same as the Kaeryangppong in cocoon yield when the freezing injury occurred 1 year out of 3 years.

• International Journal of Industrial Entomology and Biomaterials
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• v.33 no.1
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• pp.24-30
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• 2016

1-Deoxynojirimycin (DNJ) have been extensively investigated for their α-glucosidase inhibitor on postprandial hyperglycemia, and applied in nutraceuticals and medicine for preventing or delaying progression of type 2 diabetes. However, the amount of DNJ in mulberry leaves is low (about 0.1%), therefore, more effective extraction method is needed. This study was performed to develop microbial DNJ for biological methods of DNJ as an alternative to the chemical methods. In this study, we obtained evidence for Bacillus subtilis that produce DNJ in large quantities by high performance liquid chromatography. Inhibition of α-glucosidase activity was determined to DNJ production or non-production. Investigation of the effect of mulberry leaves powder concentration (1~5%), using the DNJ high-production bacteria, provided evidence for microbial mass production of DNJ. When the 4% mulberry leaf powder for 9 days was used, the α-glucosidase inhibitory activity was over the 85%. Also, the results presented in this study confirm DNJ yield's increasement in microbes using the various of nutrients and provide insight of ways to improve DNJ yields in microorganisms.

• Journal of Sericultural and Entomological Science
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• v.20 no.2
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• pp.26-31
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• 1978

The objective of the present study was to investigate the effect of nicotine-contaminated mulberry-leaf, which was grown in the vicinity of tobacco drying plant (TDP), on cocoon crop. Mulberry-leaf harvested from the field at Sericultural Experiment Station (SES), Suweon, Korea, was used as control, supposedly nicotine-free leaf. Leaf harvested from the field in the distances of 30-50m, 300-400m and 700-800m from TDP was fed during the whole larval stage of the silkworm at. SES. The effect of leaf in each treatment level on the quantitative characters of the silkworm was summarized as follows; 1. Larval duration from 4th instar on was significantly longer in the TDP-leaf treatments than for the control. 2. Duration of matured silkworm appearance became longer as the distance of the mulberry-field from TDP got shorter, because the larval duration and growth of the silkworm were not uniform in the TDP-leaf treatments. 3. Mortality rates during the late larval, cocoon spinning, and pupal stages were highest for the 30-50m leaf, especially mortality rates during the late larval and pupal stage were serious. 4. Pupation rate was lowest for the 30-50m leaf and those for the 300-400m and the 700-800m leaf were not significantly different from that of the control. 5. Nicotine damage to cocoon weight and cocoon shell weight was significant in each TDP-leaf level. Cocoon shell ratio was reduced at the same extent in each level, compared with the control. 6. The ratio among cocoon-classes was significantly different between treatments, compared with best-cocoon ratio of 87.1% for the control. Cocoons were not uniform for the 30-50m leaf, and those for the 300-400m and the 700-800m leaf were as almost uniform as those for the control. 7. Loss of fresh cocoon yield became greater as the distance of the mulberry-field from the TDP-got shorter. In conclusion, the critical distance of mulberry-field, which influences larval health, cocoon quality and yield, appeared to be 800m from the TDP. Such other factors as wind direction and topographic location may be involved in the critical distance. 8. From the present experiment, we could obtain only the effect of nicotine on the silkworm through digestive system, since the silkworm was raised at SES in Suweon. If the silkworm.. were raised in the vicinity of the TDP, poison effect of nicotine on the silkworm could beo expected through exoskeleton and tracheal system as well as through digestive system.