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http://dx.doi.org/10.7852/ijie.2016.33.1.24

Optimization of culture conditions of Bacillus subtilis with α-glucosidase inhibitory activity  

Kim, Yong-Soon (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
Ju, Wan-Taek (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
Kim, Hyun-Bok (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
Sung, Gyoo-Byung (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
Publication Information
International Journal of Industrial Entomology and Biomaterials / v.33, no.1, 2016 , pp. 24-30 More about this Journal
Abstract
1-Deoxynojirimycin (DNJ) have been extensively investigated for their α-glucosidase inhibitor on postprandial hyperglycemia, and applied in nutraceuticals and medicine for preventing or delaying progression of type 2 diabetes. However, the amount of DNJ in mulberry leaves is low (about 0.1%), therefore, more effective extraction method is needed. This study was performed to develop microbial DNJ for biological methods of DNJ as an alternative to the chemical methods. In this study, we obtained evidence for Bacillus subtilis that produce DNJ in large quantities by high performance liquid chromatography. Inhibition of α-glucosidase activity was determined to DNJ production or non-production. Investigation of the effect of mulberry leaves powder concentration (1~5%), using the DNJ high-production bacteria, provided evidence for microbial mass production of DNJ. When the 4% mulberry leaf powder for 9 days was used, the α-glucosidase inhibitory activity was over the 85%. Also, the results presented in this study confirm DNJ yield's increasement in microbes using the various of nutrients and provide insight of ways to improve DNJ yields in microorganisms.
Keywords
mulberry leaf; 1-Deoxynojirimycin (DNJ); fermentation; α-glucosidase;
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Times Cited By KSCI : 2  (Citation Analysis)
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