• Journal of Sericultural and Entomological Science
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• v.9
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• pp.27-34
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• 1969

This study was conducted investigate the effect of Gibberellin and Urea on the growth of mulberry tree with the compound treatment, and the characteristic of silkworm when the mulberry leaves simply treated with GB and the compound treatment with GB and Urea were supplied. The results are as follows. 1. Mulberry tree A. The leaf weight per mulberry tree with the compound treatment of GB+ Urea was heavier in spring silkworm and the leaf yield was increased by 7% compared with the non-treatment plot. B. The growth of wattle, leaf length and leaf width was more accelerated in the compound treatment plot of GB+Urea, the single treatment plot of GB and Urea than th!: non-treatment plot in autumn silkworm, and there was observed an incredse yielding effect of 16% in GB+Urea plot, in 16% GB plot and 18% in Urea plot respectively as compared with th: non-treatment plot, hut yield per l000m unit of wattle length was the least in GB plot. C. In the composition of mulberry leaves of treatment, water and crude protein was increased in the compound treatment plot of GB + Urea and the single treatment plot of GB and Urea than the non-treatment plot, but dry matter and carbohydrate were decredsed. 2. Characteristics of Larva in spring silkworm A. The mortality ratio of 4 and 5 instar plot was equally high in the compound treatment plot of GB+ Urea and the pupation ratio of 4 instar plot was lower than other treatment plot. B. Although there was no significant difference observed in the cocoon weight of 10.000 1st-day worms in both 4 and 5 instar plots between each treatment plot, the single treatment plot of GB showed somewhat great than other treatment plots. C. There was no significant difference observed in the cocoon layer ratio between each treatment plot, but 4 instar plots was slightly higher than 5 instar plots. 3. Characteristics of Larva in Autumn silkworm A. The mortality ratio of the compound treatment plot of GB+ Urea and GB was higher than that of the nontreatment and single treatment plot of Urea. B. The pupation ratio of the single treatment plot of Urea was higher than that of the compound treatment plot of GB + Urea, and there was no significant difference observed between other treatment plot. C. The cocoon weight of 10.000 1st-day th instar worms was heavier in the single treatment plot of Urea than nontreatment plot, GB+Urea plot and GB plot. D. The cocoon layer ratio was no significant difference observed between each-treatment.

• Journal of Korean Society of Forest Science
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• v.100 no.4
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• pp.623-629
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• 2011

The detectable levels and population fluctuations of phytoplasmas infecting dwarf mulberry trees were investigated using nested-PCR and competitive-PCR methods. Samples of five different types were studied : A. petiole of a leaf that displays dwarf symptoms, B. petiole from apparently healthy leaf residing on a branch also supports a leaf with dwarf symptoms, C. the branch portion that supports a leaf with dwarf symptoms, D. the leaf petiole from healthy appearing leaves on branch with no dwarf symptoms, and branch portion of branch with no dwarf symptoms, E. the rootlets of trees with dwarf symptoms. These 5-parts were collected from each tree during June - April, once in every two months. The phytoplasma was detected from all parts of collected mulberry samples during all seasons using nested-PCR with AS-1/AS-2 primer pairs. The phytoplasma was detected until $10^4$ dilution using direct-PCR method, but it was detected until $10^{13}$ dilution by the nested-PCR method. The density of pytoplasma was found to be $7.94{\times}10^{18}-10^{12}copies/{\mu}L$ in mulberry trees. The density of phytoplasma was observed throughout the year in all samples of mulberry trees. The highest rates of phytoplasma was found in the samples B and C during the early growing season followed by the sample A and D during the dormant season. Samples C and E displayed the highest phytoplasma density followed sample D. The density of phytoplasma appeared stable during all the seasons for samples C and A. The result of the present study demonstrates the utility of nested-PCR and competitive-PCR for detection and determination of population fluctuations of phytoplasmas in plant tissues.

• Journal of Sericultural and Entomological Science
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• v.4
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• pp.27-31
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• 1965

The aim of this work was to investigate how the mulberry shoots grew well when the mulberry branches were cut down, in hight, 1/3, $\frac{1}{2}$ and 2/3 of them, respectively, just before the Spring budding. The results obtained are as follows: 1. The rate of un-sprouted buds decreased sharply down as much as half of the control if the mulberry branches were cut off. The treatment, 1/3, was best one of various cut down sizes and the un-sprouted buds concentrated on the lower parts of the branches. 2. Even though the branches were cut down, at first it seemed flat to accelerate the sprouting of buds, but rather to have a tendency to become late. The speed of leaf growth, however, became faster after the sprouting. It took 7~9 days from the beginning of buddings to the 5th full grown leaf, and.7 days for the shortest one in the treatment, 2/3, when compared with 16 days of the control. 3. If the branches were cut down, the growth of the new sprouts was accelerated. For 45 days (on 5th of May to on 10th of June), therefore. the shoots had grown up to 1.6～2.5 times of the control, 34cm long. The shoots of which the branches were cut down at 2/3 of them were longest (83cm). Furthermore. the speed of shoot growth of cut-down branches was faster than that of the control. 4. In the increased rate of the number of the mulberry leaves, there was not significant difference between the control and the treatments. 5. The total amount of shoot growth showed considerably much more in the treatments than in the control. The successing growing shoots of treatment, 2/3 were little in a number but longest per one shoot in mean shoot length. 6. It seems to be much available that we may cut down branches at 2/3 of them in order to get the scions for the cutting as soon as possible.

• Journal of Sericultural and Entomological Science
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• no.12
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• pp.5-11
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• 1970

These works were conducted to investigate the differences of assimilatory productivity under various conditions of cultivation in mulberry trees with the punch method based on dry matter production. The results obtained are as follow： 1. The dry matter of mulberry leaves at 150cm to 200cm high in the morning and from 50cm to 100cm high in the afternoon were higher than other conditions. 2. In daily change of assimilatory, the production of the leaves from 150 cm to 200 cm high in the morning and from 50 cm to 100 cm high in tile afternoon were higher than other conditions. 3. The largest amount of leaf dry matter was produced in south outside of mulberry field, next east outside, and others showed on differences. 4. Increased assimilatory production was occured greatly not only east side of mulberry field from 7 A.M to 10 A.M but also south side of mulberry field from 10 A.M to 1 P.M. 5. In comparison of dry matter production of leaves among varieties, Suwon-sang No. 4 showed greatest amount and followed Sipyung, Kaeryangsuban, Rosang in order. In the varietal differences of assimilatory production per day, Suwonsang No. 4 was greatest and followed Kaeryrangsuban, Sipyung, Rosang in order, 6. Both of the rate of increasing leaf dry matter and net assimilation per day were greatest in the one-half cutting plot and some decreased in one-third cutting plot, in non-cutting plot in order.

• International Journal of Industrial Entomology and Biomaterials
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• v.18 no.2
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• pp.97-103
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• 2009

Sericulturists in the vicinity of Bangalore city irrigate their mulberry gardens with Vrishabhavathy stream water, which is polluted with domestic and industrial wastes from the city. To investigate the effect of pollutants on silkworm crops, a detailed field survey was conducted to study the cocoon yield pattern of the crops raised on mulberry irrigated with wastewater as against irrigation by bore/open wells. The villages along the stream were grouped into five test batches at about a distance of $5{\sim}8$ km from each other. The seasonal yield data with relevant information were collected through questionnaires from 117 rearers using stream water and 35 rearers using bore/open wells, the latter serving as control group. The average yield for 100 layings was 46 to 57 kg in the control group whereas in test groups, it ranged between 34 to 51 kg in the first test group and 22 to 38 kg in the rest. The difference in yield was $9{\sim}19$ kg depending on the season between control and test batches. In summer, this difference was higher, with high co-efficient of variation in test groups ($33{\sim}52$%). Further, water, soil and leaf samples were collected from selected rearers and were analyzed for zinc, copper, iron, lead and nickel. Results indicated significantly higher contents of these metals in samples from gardens using wastewater when compared control samples. Significantly (p<0.05 & p<0.01)) higher levels of zinc ($24{\sim}122$ ppm) and iron ($208{\sim}683$ ppm) were noticed in mulberry leaves during summer followed by winter and rainy season. The significance of high content of heavy metals in mulberry leaves and cocoon yield pattern of this area in relation to the quality of irrigation water is discussed.

• International Journal of Industrial Entomology and Biomaterials
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• v.35 no.2
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• pp.63-70
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• 2017

Native fluorescent pseudomonas bacteria were isolated from rhizosphere soil of mulberry and were evaluated against powdery mildew. In vitro conidial germination study showed significant (P<0.05) variation in conidial germination by bacterial strains Pf1 and Pf3. Mildew incidence was significantly varied due to treatment with various pseudomonas strains in vivo. Significantly (P<0.05) less mildew incidence was in plants treated with the bacterial strain Pf1 (9.11%) followed by Pf3 (13.48%) controlling 69.40% and 54.75% respectively compared with untreated control. Similarly, mildew severity was least (8.51%) in plants treated with strain Pf1 followed by Pf5 (9.23%) and Pf3 (9.72%) controlling the severity by 84.51%, 77.01% and 71.96% respectively compared with control. The bacterial strains significantly influenced biochemical constituents such as chlorophyll, protein and soluble sugar content of the mulberry leaf. Similarly, bacterial strains significantly increased the activity of the peroxidase (PO) and Polyphenol oxydase (PPO) activity from $7^{th}$ day up to the $28^{th}$ day after treatment. The strain Pf1, Pf3 and Pf5 exhibited a marked enhancement in the peroxidase at different periods of infection. Significant (P<0.01) negative correlation was found between powdery mildew severity with phenol content ($R^2=0.67$) as well as peroxidase ($R^2=0.92$) and polyphenol oxidase ($R^2=0.72$) activity thus confirms induction of systemic resistance in mulberry by pseudomonas bacteria. The study shows scope for exploration of rhizosphere fluorescent pseudomonas bacteria for induction of systemic resistance in mulberry to contain powdery mildew disease effectively.

• Proceedings of the Korean Society of Food and Cookery Science Conference
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• 2005.10a
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• pp.85-85
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• 2005

• Biomedical Science Letters
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• v.26 no.2
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• pp.75-84
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• 2020

We evaluated the effect of anti-obesitic activity of MYE (mulberry leaf + Yacon tuber) extracted from Morus alba as muberry leaf and Smallanthus sonchifolia as yacon. 1%, 3%, or 5% of MYE were treated to Sprague-Dawley rats exposed to a high-fat diet. MYE treated rats were suppressed weight during four weeks, and they lost weight significantly after six weeks. Common blood chemistry panels related to liver function revealed significant improvement in the MYE-treated groups. The expression of leptin as indicators for obesity was decreased in perirenal fat. Such results indicate that MYE could be a promising candidate for the improvement of obesity. In addition, MYE effected on deceased glucose metabolism, reducing the activities of glucose-6-phosphate dehydrogenase (G-6-PDH) and glucokinase related to glycogen synthesis. The fatty liver was observed in high-fat diet-treated rats, resulting from increased number of adipose cells and Ito cells. However, this pathologic change was significantly improved by administration of MYE. MYE have significant effects on antioxdative function and glycometabolism against high fat diet. Thereby, it seems that MYE prevent fatty liver by high-fat diet. Thus it is suggested that MYE would be worth being developed as an biofunctional food to prevent undesirable effects caused by obesity.

• Journal of Digital Convergence
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• v.15 no.1
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• pp.347-353
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• 2017

This study aims to figure out how green tea leaves, mulberry leaves, and mate leaves that are often taken around in drinking tea influence on S. mutans known as dental caries causative bacteria. After vaccinating 1% of S. mutans on the extract added badge and incubating 6 hours and 10 hours in $37^{\circ}C$, it calculated absorbance and bacterial colony number. Extract of green tea leaves and mate leaves were added with 0, 0.5, 1.0, 2.0, and 4.0% of concentration. As a result of growth suppressive effect of S. mutans, it may be observed that colony number significantly decreases in higher concentration. When it calculated bacterial colony number with adding 2% extract after 10 hours, 99.0% for green tea leaves, 97.1% for mulberry leaves, and 89.6% for mate leaves appeared.

• Journal of Sericultural and Entomological Science
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• v.32 no.2
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• pp.113-117
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• 1990

The recovery of mulberry from a late frost (April 24 to 27) was investigated for four varieties : Kaeryangppong, Cheongilppong, and Jamsang 21 in Cheongju area, Chungbuk Province. 1. Unfavourable meteorlogical conditions for recovery of mulberry followed the early late frost : lower than normal temperatures in mid-April, less sunshine in early and mid-Jay, lower temperatures and early and less precipitation in late-May, and less precipitation than normal in early June. 2. Jamsang 21 grew to the 2-3 leaf stage first, at which time Kaeryangppong and Jamsang 22 were at the 1-2 leaf stage, and Cheongilppong was at the swallow-bill shape-1 leaf stage. 3. The first leaf stage occurred on May 12, 15 days after the frost and, 19 days later in an average year. The difference lasted until the harvesting season, the 6 leaf stage. 4. Young shoot length was 50-58% of that in 1989. Kaeryangppong was the shortest at 50% and the Jamsang 21 highest at 58% of 1989 values. 5. Leaf number was 52-67% that in 1989. Kaeryangppong was the lowest at 52% and the Jamsang 21 highest at 67% of 1989 values. 6. Young shoot and leaf weight was 44-63%, and leaf weight 39-61%, compared to 1989. Kaeryangppong and Cheongilppong were the lowest, Jamsang 21 and Jamsang 22 were the highest, compared to 1989 data. 7. Young shoot length was 50-58% compared with 1989. Kaeryangppong was the lowest as 50%, the highest Jamsang 21 as 58%. 8. Number of leaf was 52-67% compared with 1989. Kearyangppong was the lowest as 52%, the highest Jamsang 21 as 67%. 9. Young shoot and leaf wight was 44-63%, and leaf weight was 39-61% compared with 1989. Kaeryangppong was the lowest, lowest, Jamsang 21 was the highest, Cheongilppong was similar with Kaeryangppong and Jamsang 22 was similar with Jamsang 21. 10. Rate of young shoot and leaf weight to total weight decreased from 75% in 1989 to 55-61% in 1990. Rate of laef weight to young shoot decreased from 72-82% to 67-71%, showed 5-10% decrease. 11. Leaf yield decreased highly in Jamsang 22 as 57% compared with 1989. Kearyangppong 61%, Jamsang 21 64%, Cheonglppong 72%. Leaf yield was 827kg/10g in Kaeryangppong, 1, 000-1, 050kg/10a in the other varieties.