• Title/Summary/Keyword: Mucous membrane

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MERCURY LEAKAGE TO SALIVA AFTER AMALGAM FILLING (아말감 충전후(充塡後) 수은(水銀)의 타액내(唾液內) 누출(漏出)에 관(關)한 연구(硏究))

  • Kim, Soo-Chul
    • Restorative Dentistry and Endodontics
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    • v.1 no.1
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    • pp.8-10
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    • 1975
  • This study is to determine or detect deposition of mercury particles emitted from dental amalgam fillings. Amalgam fillings were carried out routinely on the dogs' teeth. (CIV cavities) Mercury which contains about 200${\mu}Ci$ of $^{197}Hg$ was employed on this purpose in each cavity. Radioactivity was measured by means of well type scintillation counter from saliva and mucous membrane of surrounding tissue. The C. P. M. value from the saliva was highest, mucous membrane was moderate and least on blood and fecs, as shown table on I. II. III. IV. 1. C.P. Mvalue from Saliva reveals 114 to 135 (lowest and highest) throughout the measuring period until 41 hours. 2. From blood samples no significant value was found. 3. CPM value from feces at2nd day and 4th day was about 90. 4. The highest and lowest CPMvalue from marginal gingiva was 465 and 209 respectively. Palatine showed 281 and 189, esophagus 179 and 165, and pulp from experiment tooth 190 and 104 respectively.

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A histochemical study on the mouse oral mucous membrane toplicated with nicotin (흡연이 인체에 미치는 영향에 관한연구 -(II) Nicotin이 생쥐구강점막에 미치는 영향에 관한 조직화학적연구-)

  • Lee, Sung-Woo
    • The Journal of the Korean dental association
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    • v.10 no.10
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    • pp.654-658
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    • 1972
  • The toplications of 50% nicotin paste in oral mucous membrane of male mice weigh-ingabout 30 gram, were executed for 10, 15 and 20 days. And the tissues were observed in HE and PAS stain. The findings observed are as follows; I. Hyperplastic changes were revealed in underlying connective tissue and spinous cell layer. There were moderate vasodilatations with out edema but PAS reactions were dictinctive. in 10th day of experiment. II. There was remarkable increment of mortality up to 40percent and weight loss, but microscopic findings were not significant differences on than 10th days, in 15th day of experiment. III. Cellular anisocytosis, hyperchromatism and degenerations were observed in spinous cell layer, and vascular engergement in connec tivetissues were observed. And the mortality was up to 70 percent in 20th day of experment.

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Characteristics of Membrane Permeability on the Separation of Solid in a Liquid Livestock Manure (축분액비의 고액분리에 있어서 분리막의 투과특성)

  • 황명구;차기철;이명규
    • Journal of Animal Environmental Science
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    • v.6 no.3
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    • pp.175-184
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    • 2000
  • A lab-scale MF membrane reactor was installed to investigate the membrane permeability, characteristics of membrane fouling at each conditions, and quality of permeate (liquid livestock manure) in the separation of solid-matters using membrane. Experiment was divided three filtration type such as follows; continuous filtration, gravity filtration, and intermittent filtration. As a result of experiment, flux 1 LMH was maintained for 7days, and trans-membrane pressure(TMP) was increased gradually under 10cmHg, but it was increased immediately after 10cmHg, respectively. However, the flux was increased, the Tmax was decreased exponential more and more. During the pure-flux test, most of the fouling of membrane was reversible. At the gravity filtration, permeate could be obtained as 1.75 LMH for 3.5days without any other electronic pressure. As an investigation of membrane surface, this study could be decided that the reason of fouling at the lower flux (Run 1 and 2) was attached matters in membrane surface, but at the higher flux (Run 4-6) was concentration polarization.

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Ultrastructure of the Submandibular Gland in the Lesser White-Toothed Shrew, Crocidura suaveolens (작은땃쥐 Crocidura suaveolens 악하선의 미세구조)

  • Jeong, Soon-Jeong;Jeong, Moon-Jin;Kim, Do-Kyung;Kook, Joong-Ki;Kim, Heung-Joong;Yoon, Myung-Hee;Park, Joo-Cheol
    • Applied Microscopy
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    • v.35 no.2
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    • pp.65-72
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    • 2005
  • The ultrastructure of submandibular gland was examined in the lesser white-toothed shrew, Crocidura suaveolens. The submandibular gland of C. suaveolens was a mixed gland composed of serous and mucous acinar cells. Secretory granules from the acini were discharged through the salivary ducts into the oral cavity. Serous and mucous acinar cells had well developed rough endoplasmic reticulum and mitochondria and large amount of granules. In case of serous acinar granules, an immature granule was formless and had only dense specks, and a matured granule was a complete round type delimiting by a single membrane and had a homogeneous dense center with dense specks on the border. In case of mucous acinar granules, while an immature granule was a round type and had an only homogeneous matrix and an indistinct limiting membrane, a mature granule was an even round type having a variety of pattern with several dense bands into the homogeneous matrix and had a distinct membrane. Therefore, a mature mucous acinar granule of C. suaveolens was not only distinct from those of the other mammalian species to have a variety of pattern but also from those of C. lasiura to have an even round type. A great serous-like secretory granules and Myelin-like body were observed in the cytoplasm and lumen of granular duct cells. Myelin-like body, a characteristic structure only reported in salivary gland of three shrews, was discharged from secretory cell into lumen by the manner of exocytosis which has little differences from discharging manner of secretory granules.

Fine Structure of the Integumentary Supporting Cell and Gland Cell of the Sea Bass, Lateolabrax japonicus (Teleostei: Moronidae) (농어 (Lateolabrax japonicus) 피부계 지지세포와 선세포의 미세구조)

  • Lee, Jung-Sick;Kim, Jae-Won
    • Applied Microscopy
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    • v.29 no.4
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    • pp.417-426
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    • 1999
  • The present investigation is designed to provide basic information on fine structure of the skin of the sea bass, Lateolabrax japonicks in relation to study of epidermal change with environmental and physiological change. The skin of the sea bass is divided into the epidermal layer and dermal layer. Epidermal layer consists of supporting cells and unicellular glands. The supporting cells were classified into the superficial cell, intermediated cell and basal cell. Gland cells were classified into the mucous secretory cell and club cell which is more frequently observed. Superficial cell of epidermal layer is squamous or cuboidal and contains well-developed rough endoplasmic reticulum and the surface is covered with numerous microridges. Superficial cells are connected to another cell with membrane interdigitations and desmosomes. Intermediated cell is ovoid and the electron density is higher than the other supporting cells. Basal cell is cuboidal and has a well-developed mitochondria and membrane interdigitation. The mucous secretory cell has a numerous membrane bounded secretory granules. The cytoplasm of club cell is divided into cortex and medullar. The medullar cytoplasm has a nucleus, intracellular organelles and central vacuole, and the cortical cytoplasm has a well-developed tonofilament. Club cells are connected to another cell with well -developed membrane interdigitations and desmosomes.

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Vaginal Endoscopy for Detecting Estrual Cycles and developing Artificial Insemination Technique in the Bitch (개에서 내시경을 이용한 발정주기 검사 및 인공수정 기법의 실험조사)

  • 신남식;문유식;최윤주;정동희;김용준;권오경
    • Journal of Veterinary Clinics
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    • v.14 no.1
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    • pp.93-98
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    • 1997
  • The vaginal endoscopy was performed in two Labrador Retrievers from onset of vaginal bleeding until metestrus accompanying with vaginal smear and blood progesterone concentration to investigate estral changes, and the intrauterine infusion of radiopaque fluid using vaginal endoscope (rigid 25-degree endoscope, Karl Storz) was detected by radiogrphy to determine if theis technique is feasible for future artificial insemination in the dogs. During the proestrus vaginal mucous membrane folds were edematous and a large amount of clear red discharge was present. In the beginning of estrus, the mucous membrane folds was present and the amount of the red discharge was decreased. In the estrus there were obbvious shrinkage, angulation of the membrane folds and decreasing of the discharge. In metestrus the vaginal folds started to round out and the surface appeared moist and sticky. The endoscope used for endoscopic monitoring of the vaginal mucosa was advanced until the external os of the cervix could be visualized. A plastic catheter, 8Fr guage was manupulated into the cervical os and then advanced through into the uterus. The radiopaque fluid was injected into the uterus through the catheter and radiographs taken to detect the site of insemination using the endoscopic technique revealed the exact infusion of the fluid in the uterus, consequentlyto prove that this technique is usable for more successful artificial insemintion tn the bitch.

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Effects of Kumsooyukkun-jeon on Asthma Induced by Ovalbumin in Mice (금수육군전이 Ovalbumin으로 수발된 Mice의 천식에 미치는 영향)

  • 김춘석;최해윤;김종대
    • The Journal of Korean Medicine
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    • v.23 no.3
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    • pp.104-118
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    • 2002
  • Objectives : To study the effects of Kumsooyukkun-jeon on asthma. Methods : Asthma was induced to Balb/c mice with ovalbumin using the method of Hatfield et al. We measured the histological profiles of lung and trachea, numbers of cellular compartments in the bronchoalveolar lavage fluid (BALF), numbers and morphology of the mast cells in the trachea, numbers of mucous-secretory cells in the bronchus, morphology of the bronchus, ultramicroscopical appearance of surface of trachea and number of cilia and mucous-secretory cells by scanning electron microscope. Results : 1. Hypertrophy of mucous membrane of trachea and bronchus and bronchioles in the lung, peritracheal, peribronchus and peribronchiolar inflammatory cell infiltration, and mucoid exudate deposition the lumen were observed in control groups but these phenomena were recovered in the Kumsooyukkun-jeon groups. 2. Cellular compartments including neutrophil and eosinophil were increased in the BALF of control groups but these phenomena were recovered in the Kumsooyukkun-jeon groups. 3. Degranulation and decrease of the numbers of mast cells were detected in the trachea of control groups. However, these phenomena were recovered in the Kumsooyukkun-jeon groups. 4. Shedding, decrease of cilia cell and increase of mucous-secretory cells in the surface of the trachea were measured in control groups but these phenomena were recovered in the Kumsooyukkun-jeon groups. Conclusions : It is considered that Kumsooyukkun-jeon has somewhat favorable effects on asthma.

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Clinical evaluation of sinus bone graft in patients with mucous retention cyst

  • Kim, Seong-Beom;Yun, Pil-Young;Kim, Young-Kyun
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.38
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    • pp.35.1-35.5
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    • 2016
  • Background: Mucous retention cyst refers to a cyst made by expansion due to the blockage of the salivary gland near the maxillary sinus, and it is surrounded by epithelial cells. Most of them are small; therefore, they cannot be found well and are frequently with antral polyp. The aim of this study was to evaluate the clinical prognosis of sinus bone graft in patients with mucous retention cyst. Methods: This study was performed retrospectively on 23 patients who had sinus bone graft. Group 1 was 8 patients (10 sinuses) who had a mucous retention cyst, and group 2 was 15 patients (17 sinuses) who had no pathologic history about the maxillary sinus. For these patients, sinus bone graft was performed using the lateral approach technique. The total 51 implants were placed 6.22 weeks on the average after sinus bone graft. Sinus membrane perforation during operation, postoperative complications, marginal bone loss after restorative function, implant success rate, and survival rate were analyzed. Results: There was no complication in group 1, and there were three complications in group 2. In group 2, two cases of implants failed. The types of postoperative complications consisted of two minor infections and one wound dehiscence. Two implants of total 51 implants were removed, and the survival rate of implants was 96.08 % (group 1 100 %, group 2 93.5 %). The total success rate of implants was 92.2 % (group 1 95 %, group 2 90.3 %). Conclusions: The clinical prognosis was not affected by the presence of mucous retention cyst.

Ultrastructural and Histochemical Studies on the Epithelial Cells and Mucus-producing Cells of Korean Slug(Limax flavus L.) (한국산 노랑민달팽이(Limax flavus L.)의 표피상피세포와 점액형성세포에 관한 미세구조 및 조직화학적인 연구)

  • Chang, Nam-Sub
    • Applied Microscopy
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    • v.18 no.2
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    • pp.1-20
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    • 1988
  • The species of the slug used in the experiment is Limax flavus L. For identifying the chemical characteristics of the epidermis, granules and mucus-producing cell of this animal is examined with methylene blue-basic fuchsin double stain and PAS-alcian blue reagent. For the ultrastructural research of the epidermal free surface, the epitheial cell and the parenchymal cell are used with scanning electron microscope and transmission elec-tron microscope respectively. I . Epidermal tissue The epidermal tissue of the slug is observed being divided into the dorsal and the ventral side(toot pad) respectively. 1) Dorsal epidermal tissue The dorsal epidermis of the slug is constituted with the simple columnar epithelium and the microvilli are compacted on the epidermal free surface. Two different types of the secretory granules of the neutral and the acid mucus are observed between the epithelial cells, and the neutral mucous granules are highest electron-dense but the acid mucous granules are observed to be electron-lucent. 2) Foot epidermal tissue The Foot epidermis is formed with the taller simple columnar epithelium than the dorsal epidermis and these cells have both a large number of the microvilli and a few number of the large villi. The secretory granules of three different types, which are acid, neutral and mixed mucous granule of two different types are observed between the epithelial cells. The neutral mucous granules are highest electron dense but the acid mucous granules are observed to be electron-lucent. II . Mucous granule-producing cell and mucus-producing cells Seven different types of the granules-producing cell and the mucus-producing cells are observed between the parenchyma. 1) A-type of acid mucous granule-producing cell The electron-lucent granules are largely occupied in the cytoplasm of these cells and then the granules are surrounded by irregular membrane. These electron-lucent granules exhibit alcianophilia with PAS-alcian blue reaction, so these granules are certified to be acid mucopolysaccharide. 2) B-type of acid mucus-producing cell The nucleus and the cytoplasm of these cells are pushed by the acid mucus of the electron-lucent toward the cell membrane. This mucus has been confirmed to be the acid mucopolysaccharide with PAS-alcian blue reagent. 3) A-type of neutral mucous granule-producing cell These cells contain the electron-dense round granules with approximately $1{\mu}m$ in diameter, which exhibit strongly PAS-positive reaction. These granules are confirmed to be the neutral mucoplysaccharide. 4) B-type of neutral mucous granule-producing cell These cells contain two different types of electron dense granules and electron-lucent granules; The former exhibits to be strongly PAS-positive and the latter to have alcianophilia reaction respectively. 5) C-type of neutral mucus-producing cell These cells are similar to the shape and the size of the B-type of mucus-producing cell but these two different types of cells are stained with reversing properties to each other. The mucus of the C-type cell that electron-lucent is largely occupied in the cytoplasm that exhibits strongly PAS-positive reaction. 6) D-type of neutral mucous granule-producing cell These cells contain round granules about $1{\mu}m$ in size which are observed to be medium electron-dense granules and those granules are stained brightly red with PAS-weak positive reaction. The granules are certified to be neutral mucopolysaccharide. 7) E-type of neutral mucous granule-producing cell These cells are similar to the shape and the size of the D-type of neutral mucous granule-producing cell. These cells contain a large number of granules with about $1{\mu}m$ in diameter showing electron-lucent and then granules are seen to be PAS-weak positive reaction. III. Parenchyma The clear cell and dark cell are found in the parenchyma of the Limax flavus L. 1) Clear cell These cells are round formed and the nucleus of the cells are larger than cytoplasm. These cells which have the electron-lucent cytosol possess poorly developed organelles. 2) Dark cell These cells are found to be dark cells due to high electron-density, which exhibit strongly methylene-blue reaction from double stain of methylene blue-basic fuchsin.

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