• 한국수산과학회지
• /
• 제33권2호
• /
• pp.148-152
• /
• 2000

베도라치의 피부는 크게 상피층과 진피층으로 구분된다. 상피층은 지지세포와 선세포들로 구성된다. 지지세포는 표면세포, 중간세포 및 기저세포로 구분되며, 선세포는 점액분비세포와 곤봉랑 세포로 구분된다. 지지세포들의 세포질은 피질부와 수질부로 나누어지는데, 수질부에는 세포소기관의 발달이 현저하며, 피질부에는 미세섬유의 발달이 뚜렷하다. 표면세포는 입방형이며 핵은 말굽형이다. 중간세포는 불규칙한 형태의 핵을 가지며, 핵과 세포질의 전자밀도는 지지세포 가운데 가장 높다. 기저세포는 원주형이나 핵은 세포질의 위쪽에 위치한다. 점액분비세포의 세포질은 타원형의 분비과립이 대부분을 차지하며, AB-PAS에 적색으로 반응하였다. 조면소포체와 골지체가 발달되어 있다. 곤봉상 세포의 세포질 상부에는 원형의 분비성 물질들이 차지하며, 핵은 장방형이고, 세포질에서는 조면소포체와 활면소포체가 발달되어 있다. 색소세포는 세포질에 전자밀도가 높은 색소과립들을 함유한 종류와 반사소판을 함유한 종류로 구분된다. 섬유세포의 세포질은 아주 잘 발달된 조면소포체들이 대부분을 차지한다.

• Applied Microscopy
• /
• 제35권2호
• /
• pp.65-72
• /
• 2005

악하선의 미세구조를 작은땃쥐 Crocidura suaveolens에서 연구하였다. 작은땃쥐의 악하선은 장액선세포와 점액선세포로 구성된 혼합샘이었다. 이 샘포에서 분비된 과립들은 도관을 거쳐 구강으로 분비되었다. 장액선세포와 점액선세포는 잘 발달된 조면소포체와 미토콘드리아 그리고 많은 분비과립을 가지고 있었다. 장액선 분비과립의 경우, 미성숙 분비과립은 무형이면서 전자밀도가 있는 작은 알갱이로만 구성되었고, 성숙 과립은 단일막으로 싸여진 완전한 원형으로 전자밀도가 있는 균질의 중앙부와 전자밀도가 있는 작은 알갱이로 구성된 주변부를 가지고 있었다. 점액선 분비과립의 경우, 미성숙 과립은 원형으로 균질한 기질과 불명확한 경계막을 가지는 반면, 성숙 과립은 균질한 기질 내에 몇 개의 전자밀도가 있는 띠를 가짐으로서 문양의 다양성 가지는 매끈한 원형이었고 명확한 경계막을 가지고 있었다. 즉 작은 땃쥐의 성숙 점액선 분비과립은 문양의 다양성을 가져 다른 포유류와 구분될 뿐만 아니라 매끈한 원형이어서 C. lasiura의 그것과도 구분되었다. 거대한 분비 과립과 미엘린소체가 과립관세포의 세포질과 내강에서 관찰되었다. 3종의 땃쥐류 침샘의 과립관에서 만 보고된 특징적 구조물인 미엘린소체는 분비세포에서 내강으로 분비되었으며 분비과립의 배출방식과 약간의 차이점을 가졌다.

• 한국임상수의학회지
• /
• 제2권1호
• /
• pp.79-104
• /
• 1985

Clinical and cytomorphological studies were carried out in 32 leukotic cattle from Tokachi and Kushiro districts in Hokkaido during the 12 year period from 1969 to 1980. The leukotic cattle were examined :and divided into four types(15 cases of the adult, 11 cases of the thymic, 4 casas of the calf and 2 cases of the skin types). The results obtained were as follows : 1. As for the frequency of the main clinical signs in each type, In the adult type, the main clinical signs (of decreasing order) are as follows: swelling of the superficial lymph nodes>depression and loss of weight>tachycardia>anorekia, anemia of the visible mucous membrane and tachypnea. Those of the thymic type were swelling of the thymus>swelling of the medial iliac lymph nodes> swelling of the superficial lymph nodes>tachypnea. Those of the calf type were swelling of the auperficial lymph nodes>depression and emaciation>tachypnea>anorexia, tachycardia, anemia of the visible mucous membrane and recumbency. Those of the skin type were generalized urticaria-like lesions in skin and swelling of superficial lymph nodes>and depression and loss of weight in the decreasing order of frequency. In addition, large tumor mass in the pelvic cavity and swelling of the medial iliac lymph nodes were detected through rectal palpation in 33.3% and 100% in the adult type cases, respectively. 2. As for the hematological findings, The frequency of occurrence of decreased erythrocyte counts in the decreasing order were as follows : adult>calf>thymic>and skin types. The increase in the total leukocyte count in the order of decreasing frequency were as follows: calf>thymic>adult>and skin types. The increase in the absolute lymphocyte counts was found to be at a low rate, 62.5% of all the cases examined. By contrast, the increase of 5% or more of abnormal lymphocyte rates was observed at a high rate, 96.9% of the total cases. 3. Abnormal lymphocytes were found in all cases examined for lymph nodes biopsied. 4. From the cytomorphological point of view, leukotic cells were divided into 3 types: reticulum cell, lymphoid cell and monocytic cell types. The adult type leukotic cattle were divided with reticulum cell type (66.7%), the lymphoid cell type(22.6%) and monocytic cell type(6.7%). The thymic type was lymphoip cell type(72.7%) and reticulum cell type(27.3%). In the calf type, all were lymphoid cell type while all of the skin type were reticulum cell type only. 5. The leukotic cattle had higher NP frequency in the blood and lymphoid tissue than non-1 eukotic cattle. Especially the adult type had the highest NP frequency. However, it was not recognized that NP were characteristic of leukotic cattle alone. 6. The above findings lead to the conclusion that the most effective diagnostic methods for bovine leukosis are the confirmation of swelling of the superficial and internal lymph nodes and thymus in addition to appearance of abnormal neoplastic cell in the peripheral blood and lymph nodes biopsied.

• 한국어류학회지
• /
• 제19권2호
• /
• pp.120-127
• /
• 2007

한국산 미끈망둑(Luciogobius guttatus)의 피부구조가 머리, 위턱, 아래턱, 뺨, 등, 체측, 배, 아가미뚜껑 등 모두 8개 부분에서 비교 조사되었다. 이들 모두 표피는 맨바깥층, 중간층, 기저층으로 구성되었다. 맨바깥층은 1~4층의 편평세포와 점액세포로 구성되었으며, 중간층은 1~10층의 대형 표피세포를 가지며 이러한 표피세포는 마치 거미줄 같은 형태를 보여주고 있다. 이러한 대형표피세포의 존재는 두꺼운 표피를 갖도록 하며, tonofiilament와 desmosome을 가진다. 한편 기저층은 한 층의 입방상피로 구성되어 있으며, 많은 모세혈관들이 기저층 바로 아래부분에 존재한다. 진피에는 비늘이 존재하지 않으며 거의 대부분 모세혈관을 가지는 치밀층으로 구성되어 있다.

• 한국패류학회지
• /
• 제29권1호
• /
• pp.7-13
• /
• 2013

본 연구는 납에 노출된 바지락, Ruditapes philippinarum 의 생물학적 반응을 알아보고자 하였다. 실험기간은 4주였으며, 실험구는 대조구 1개와 납 노출구 3개 (0.25, 0.50 and 1.00 mg/l) 였다. 실험 결과 납은 바지락의 생존율과 산소 소비율의 저하 및 기관계의 조직학적 변성을 유도하는 것이 확인 되었다. 납 노출구에서 산소소비율은 25-72% 감소하였다. 기관계의 조직학적 분석 결과, 외투막 상피층과 결합조직층의 변성, 아가미 점액세포의 증가와 상피세포의 괴사, 발에서는 상피층의 붕괴, 점액세포의 감소 및 혈림프동의 확장과 결합조직층의 변성을 나타냈다. 소화맹낭의 소화선세관에서는 호염기성세포와 상피세포의 위축 및 농도 의존적으로 lipofuscin의 축적이 확인되었다.

• Applied Microscopy
• /
• 제34권2호
• /
• pp.83-93
• /
• 2004

붉은쏨뱅이의 피부 상피층은 다층상피층으로 지지세포, 선세포, 과립세포 그리고 mitochodria-rich cell들로 구성된다. 상피층은 지지세포의 형태와 구조에 따라 표면층, 중간층 및 기저층으로 구분할 수 있다. 점액세포들은 단세포선으로 상피의 표면층과 중간층에 분포하며, 산성의 비황화 뮤코다당류로 구성된 점액을 가진다. 곤봉상세포는 세포질에 잘 발달된 중심공포, 조면소포체 및 골지체를 가진다. 과립세포는 표면층에 존재하며, 전자밀도가 높은 과립을 가진다. mitochodria-rich cell의 주변부 세포질에는 미세섬유 다발이 발달되어 있으며, 수질부에는 다수의 관상 미토콘드리아를 가진다. 색소세포는 세포질에 존재하는 함유물의 전자밀도에 따라 세 종류로 구분할 수 있다.

• 대한한의학회지
• /
• 제23권4호
• /
• pp.169-185
• /
• 2002

Objectives: This study was carried out to investigate 1he effects of Sasammaickmoondong-tang(SME) on colonic mucosal lesions induced by indomethacin in mouse. Methods: The normal group is 1hat no inflammation elicitated mouse. Control group is that gastro-inflammation elicitated mouse. Sample group is that SME administered mouse after gastro-inflammation elicitation. Results: In the common morphology and histochemical change, control group was observed various injury-mucous surface cell, micro-villi, paneth cell, surface epithelial cell, goblet cell-by hemorrhagic erosion, while sample group was as same as normal group. In the immunohistochemical change, 1he distributions of COX-1, Bcl-2, and BrdU treated with SME noticeably increased than control group(P<0.05). The distributions of TUNEL, $NF-{\kappa}B$, COX-2, $IL-2R-\alpha$, NK-1.1, ICAM-1, and CD11b/18 treated with SME noticeably decreased than control group(P<0.05). And the distribution of SBA was as same as normal group. Conclusions: According to the above results, it is supposed that Sasammaickmoondong-tang is applicable to colonic mucosal lesions.

• 대한한의학회지
• /
• 제24권2호
• /
• pp.121-137
• /
• 2003

Objectives: This study was carried out to investigate the effects of Sasammaickmoondong-tang (SME) on gastric mucosal lesions induced by indomethacin in mice. Methods: The normal group was no inflammation-induced mice. The control group was gastro-inflammation-induced mice. The sample group was mice administered SME after gastro-inflammation elicitation. Results: In the common morphology and histochemical change, the control group was observed with various injury-mucous surface cell, micro-villi, paneth cell, surface epithelial cell, goblet cell - by hemorrhagic erosion, while the sample group was as same as the normal group. In the immunohistochemical change, the distributions of COX-1, Bcl-2, and BrdU treated with SME were noticeably higher than in the control group (p<0.05). The distributions of TUNEL, NF-B, COX-2, IL-2R-, NK-1.1, ICAM-1, and CD11b/18 in those treated with SME were noticeably lower than in the control group (p<0.05). Finally, the distribution of SBA was the same as in the normal group. Conclusions: According to the above results, it is supposed that Sasammaickmoondong-tang is applicable to gastric mucosal lesions.

• 대한치과의사협회지
• /
• 제52권12호
• /
• pp.726-733
• /
• 2014

The gingiva consists of an epithelial layer and an underlying connective tissue layer. The oral epithelium is a keratinized, stratified, squamous epithelium. The epithelium can be divided into the following cell layer: basal layer, prickle cell layer, granular cell layer and keratinized cell layer. The desquamative disease of gingiva means exfoliative diseases of epithelial layer on the gingiva. The chronic desqumative gingivitis is usually related to the dematologic disorders that produce cutaneous and mucous membrane blisters. The cicatricial pemphigoid and lichen planus are representative diseases of the dermatologic cases. Patients may be asymptomatic or symptomatic. When symptomatic, their complaints range from a mild burning sentation to an severe pain. The clinical examination must be considered with a thorough history, and routine histologic and immunofluorescence studies. A systemic approach needs to achieve accurate diagnosis and treatment of the gingival desquamative diseases.

• Applied Microscopy
• /
• 제22권2호
• /
• pp.15-29
• /
• 1992

The gallbladder is known to have the function of the storage and the concentration of the bile produced by the liver. This function is carried out by the removal of water and inorganic electrolytes. Extrahepatic cholestasis or the impairment of excretion of the bile leads to the distension and loss of the function of the gallbladder. The purpose of this study was to examine the ultrastructural characteristics of the normal gallbladder epithelial cells, and their structural changes induced by the ligation of common bile duct of the rabbit. Common bile duct ligation was performed under ether anesthesia. The rabbits were sacrificed on the 1st, 3rd, 5th, 7th and 14th day, respectively after operations. The tissue blocks of the gallbladder were fixed in 2.5% glutaraldehyde-1.5% paraformaldehyde prior to fixation in 1% osmium tetroxide, and embedded in the araldite mixture, and observed with JEM 100 CX-II electron microscope. The results were as follows: 1. The normal gallbladder epithelium of adult rabbit demonstrated two cell types, the ordinary epthelial cell and the dark cell. The dark cells have electron dense cytoplasm, and were found much infrequently, whereas ordinary epthelial cells were found quite numerous. 2. The ordinary epthelial cells of normal gallbladder were provided with the regular microvilli at the free surface and the images of pinocytotic activities in the apical cytoplasm, and exhibit highly convoluted lateral surfaces with elaborated microfolds. These figures of the cells suggest that they are resorptive in functional activity. 3. In the early stages (1st, 3rd, 5th day groups) following the ligation, the apical cytoplasm of some cells is protruding from the free surface and lost their microvilli. Numerous mucous granules filled in the apical and supranuclear cytoplasm compactly. 4. In the late stages (7th, 14th day groups) following the ligation, many light cells containing mumerous mucous granules are seen, between the ordinary epthelial cells. Mucous granules are fused each other, and are discharged into the lumen from the apical cytoplasm. The lateral membranes are straight or undulating without any interdigitations. From the above results, it was concluded that in the cholestasis induced by the common bile duct ligation, there is a tendency for the mucosal epithelium of the rabbit gallbladder to have secretory rather than an absorptive function.