• Development and Reproduction
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• v.6 no.1
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• pp.31-35
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• 2002

The major urinary proteins(MUPs) of mice that bind hydrophobic molecules known as pheromones are regulated in part by the actions of growth hormone. The expression of the MUPs was therefore investigated in transgenic mice that express a human growth hormone-releasing factor gene from a metallothionein gene promoter(MT-GRF) and as a result have elevated growth hormone levels. MUPs were severely down-regulated in the urine of these animals compared to normal mice or to control transgenic mice expressing another gene(the inhibin a subunit) from the same metallothionein promoter(MT-Inh) and more MUPs disappeared in male mice than female ones. MUPs were also down-regulated in the urine of the UT-GRF-injected mice. In addition, it was observed that the urine of the MT-GRF mice included a high molecular weight protein that co-migrates with the major serum protein albumin, indicating an impairment in glomerular filtration within the kidney. The urinary loss of serum proteins was more severe in male MT-GRF mice than female ones. Thus the overexpression of human GRF mimics changes observed in MUP protein expression and glomerular function in other models of growth hormone hypersecretion with sex-dependent differential effects.

• Environmental Analysis Health and Toxicology
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• v.19 no.4
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• pp.389-399
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• 2004

Metallothionein (MT), a small protein molecule which can bind or release metal ions, is involved in the regulation of cellular metal homeostasis. This study was investigated the accumulation of cadmium in blood, tissue (liver, kidney and brain), and the effect of cadmium on several key genes (MT-I, MT-II, ZnT-1) in zinc metabolism in the mouse. Mouses weighing 20∼25 g were randomly assigned to control and cadmium treated group (Cd group). Cd group was intraperitoneally injected with cadmium 2, 4, 8 mg/kg and control group was administerd with saline. Mouses of each group were sacrificed by decapitation 4 hours after the administration of cadmium. Cadmium contents in blood, liver, kidney and brain were increased by a dose-dependent manner. Accumulation of cadmium was mainly occurred in liver and kidney. Induction of MT-I and MT-II protein was increased, but ZnT-1 expression was decreased in a dose-dependent manner by the treatment of 2∼8 mg/kg cadmium. These results suggested that cadmium can be transported to brain and alter the expression of several key genes in zinc homeostasis.

• Journal of the Korean Institute of Landscape Architecture
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• v.21 no.3
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• pp.77-88
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• 1993

This study is a fundamental research to propose the preservation and management technique of urban skylines in case of Seoul City, and to find landscape resources that based on investigation of characteristics of urban landscape. The question are is designed for citizens' perception of urban landscaped. The results of this study is summarized as follows.; 1) The representative landscapes in Seoul City are chiefly Mt.Nam, Hand River, 63 Bldg, Kyungbokgung, Namdaemum, etc. The difference of the reasons of reasons of chosing for representative landscapes is that there must be a certain difference in direction of preservation and management of the landscape. 2) The ward which don't have obvious representative landscapes requires process survey and designation for representative landscapes, and technique for strenghtening them. 3) The major places of viewing the impressive or beautiful landscape are classfied into places of viewing whole Seoul City(ex;Mt.Nam, 63 Bldg, Seoul Toewr, MT.Kwanak, etc) and places of viewing Han River(ex;Youido Square, Olympic Road, Riverside parks of Han River etc). 4) For conservation and management of urban landscapes, it is requisitive for harmony of preservation and management technique between Seoul City and each ward.

• Korean Journal of Ichthyology
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• v.23 no.1
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• pp.61-69
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• 2011

Gene and promoter structures of metallothionein(MT) from Miho spine loach (Cobitis choii; Cypriniformes) were characterized, and the transcriptional responses to experimental exposures to heavy metals and heat stress were examined. The C. choii metallothionein displayed well-conserved features of teleostean metallothioneins at gDNA, mRNA and amino acid levels. Bioinformatic analysis predicted that the C. choii MT regulatory region potentially possessed various motifs or elements targeted by various transcription factors associated with metal-coordinating regulation (e.g., metal transcription factor-1), immune responses (e.g., nuclear factor kappa B), and thermal modulations (e.g., heat shock factor). Acute heavy-metal exposures to 0.5 or $1.0\;{\mu}M$ of cadmium (Cd), copper (Cu), manganese (Mn), nickel (Ni) or zinc (Zn) showed that MT transcription was significantly stimulated by Cd (9.6-fold relative to non-exposed control) and Cu (10.4-fold), only moderately by Mn (2.4-fold), but hardly by Ni and Zn. Elevation of water temperature from $25^{\circ}C$ to $31^{\circ}C$ caused a rapid modulation of MT mRNAs toward upregulation to 9.5-fold; however, afterward the elevated mRNA level slightly decreased during further incubation at $31^{\circ}C$ for 6 h. Results from this study suggest that MT-based expression assay could be a useful basis for better understanding the metal- and/or heat-caused stresses in this endangered fish species.

• Korean Journal of Ichthyology
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• v.23 no.1
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• pp.10-20
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• 2011

Genetic determinant for metallothionein (MT), a cysteine-rich protein playing essential roles in metal detoxification and homeostasis, was characterized in the Korean bitterling (Acheilognathus signifer, Cyprinidae), an endemic fish species. The full-length A. signifer MT (AsMT) cDNA (551 bp) is composed of a single open-reading frame (ORF) to encode a polypeptide of 60 amino acids containing 20 cysteine residues whose positions are conserved in most cypriniform MTs. At the genomic level, the AsMT (2,593 bp spanning the 5'-flanking region to the 3'-untranslated region) represented a conserved tripartite (three exons interrupted by two introns) structure with AT-rich introns. The upstream regulatory region (-1,914 bp from the ATG initiation codon) of AsMT displayed various sites and motifs for transcription factors involved in the metal-mediated regulation and stress/immune responses. The AsMT transcript was ubiquitously detected in various organs with variable expression levels, where the ovary and intestine showed the highest expression, while the heart and skeletal muscle represented the lowest level. During an exposure to copper (immersion in $0.5\;{\mu}M$ Cu for 48 h), the levels of AsMT transcripts were significantly elevated in the liver (more than 3.5-fold), moderately in the gill, kidney, and spleen (ranging from 1.5- to 2.5-fold), and barely in the brain and intestine. Results of this study could form a useful basis to explore the metal-related stress physiology of this endangered fish species.

• Proceedings of the Plant Resources Society of Korea Conference
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• v.18 no.1
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• pp.66-66
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• 2005

• The Korean Journal of Ecology
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• v.6 no.4
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• pp.33-59
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• 1983

An investigation has been performed on the flora and life form of the vascular plants at Mt. Sori in Kwangneung from March to November in 1982. In a survey field trip, 539 species, 344 genera, 102 families of plants. are collected. Among them Hylomecon vernale, Aconitum coreanum, A. pseudoproliferum, Adonis amurensis, Anemone raddeana is important plant in this area and they are becoming sparse than past. In the life form spectrum hemiryptophytes is 40.6%.

• 한국지구물리탐사학회:학술대회논문집
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• 2010.10a
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• pp.63-66
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• 2010

• Journal of the Korean Society of Environmental Restoration Technology
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• v.18 no.6
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• pp.201-214
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• 2015

This study was conducted to identify forest topsoil's usefulness from July 2013 to Oct 2014. Generally, there are abundant seeds in topsoils and it can be used at restoration works. Mt. GARIWANG is famous for biodiversity in Korea. This study was aimed to find out the suitable utilization methods of natural topsoils for restoration works at sky-slopes construction sites. Test beds was made to identify suitable collecting times and suitable topsoil thickness. The main results are summarized as follows. First, Mt. GARIWANG have diverse species and in topsoils we can find lots of burried seeds which can be used at restoration works. Second, according to indoor experiment, as the depth of topsoil used thicker, the number of plants and coverage rates were increased. Also, there were no distinct differences in coverage rates between 5cm and 8cm thick topsoil plots and it can be possible to use at least 3cm thick topsoils even thou lower coverage rates. Third, as the results of research about indoor and outdoor experimental plots, there were difference in plant emergency. If we use the stabilizer to protect soil erosion, then we can get more increased results at sloped outdoor experimental plots. Fourth, based on environmental impact assessment, there was 389 species in whole region of Mt. GARIWANG. Through this seed bank experiments, we can find 23 varieties, including 4 tree varieties and 19 herbaceous varieties. We can find lots of native herbaceous species from topsoils. Fifth, this research was done at the specific area of Mt. GARIWANG and used limited topsoils. But we monitoring more longer periods, then there will be more useful outcomes. Finally by researching topsoils of Mt. GARIWANG, we can find diverse native plants. Thus, we must reuse natural topsoils of Mt. Gariwang for restoration of original vegetation.

• Geophysics and Geophysical Exploration
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• v.10 no.4
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• pp.259-267
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• 2007

An efficient algorithm for inverting static-shifted magnetotelluric (MT) data has been proposed to produce a three-dimensional (3D) resistivity model. In the Gauss-Newton approach, computational costs associated with construction of a full sensitivity matrix usually make 3D MT inversion impractical. This computational difficulty may be overcome by using approximate sensitivities. We use four kinds of sensitivities in particular orders in the inversion process. These sensitivities are computed 1) analytically for an initial, homogeneous earth, 2) exactly for a current model, 3) approximately by the Broyden method, and 4) approximately using the previous adjoint fields. Inversion experiments with static-shifted synthetic and field MT data indicate that inversion results are highly dependent on characteristics of data and thus applying various combinations of sensitivities is helpful in obtaining a good image of the subsurface structure with reasonable computation time.