• Journal of Life Science
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• v.21 no.9
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• pp.1226-1233
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• 2011

In neurons, kinesin is the molecular motor that transport cargos along microtubules. KIF5s (alias kinesin-I), are heterotetrameric motor conveying cargos, but the mechanism as to how they recognize and bind to a specific cargos has not yet been completely elucidated. To identify the interaction proteins for KIF5C, yeast two-hybrid screening was performed, and specific interaction with the $\underline{S}$am68-$\underline{l}$ike $\underline{m}$ammalian protein $\underline{2}$ (SLM-2), a member of the $\underline{s}$ignal $\underline{t}$ransducers and $\underline{a}$ctivators of $\underline{R}$NA (STAR) family of RNA processing proteins, was found. SLM-2 bound to the carboxyl (C)-terminal region of KIF5C and to other KIF5 members. The C-terminal domain of Sam68, SLM-1, SLM-2 was essential for interaction with KIF5C in the yeast two-hybrid assay. In addition, glutathione S-transferase (GST) pull-downs showed that SAM68, SLM-1, and SLM-2 specifically interacted to Kinesin-I complex. An antibody to SAM68 specifically co-immunoprecipitated SAM68 associated with KIF5s and coprecipitated with a specific set of mRNA. These results suggest that Kinesin-I motor protein transports RNA-associated protein complex in cells.

• Journal of Life Science
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• v.22 no.12
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• pp.1608-1613
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• 2012

A conventional kinesin, KIF5/Kinesin-I, transports various cargoes along the microtubule through interaction between its light chain subunit and the cargoes. Kinesin light chains (KLCs) interact with many different cargoes using their tetratricopeptide repeat (TPR) domain, but the mechanism underlying recognition and binding of a specific cargo has not yet been completely elucidated. We used the yeast two-hybrid assay to identify proteins that interact with the TPR domain of KLC1. We found an interaction between the TPR domain of KLC1 and an amyloid precursor protein (APP)-binding protein PAT1 (protein interacting with APP tail 1). The yeast two-hybrid assay demonstrated that the TPR domain-containing region of KLC1 mediated binding to the C-terminal tail region of PAT1. PAT1 also bound to KLC2 but not to kinesin heavy chains (KIF5A, KIF5B, and KIF5C) in the yeast two-hybrid assay. These protein-protein interactions were also observed in the glutathione S-transferase (GST) pull-down assay and by co-immunoprecipitation. Anti-PAT1 antibody as well as anti-APP anti-body co-immunoprecipitated KLC and KHCs associated with PAT1 from mouse brain extracts. These results suggest that PAT1 could mediate interactions between Kinesin-I and APP containing vesicles.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.9 no.3
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• pp.257-265
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• 2016

In this study, an optimal design for motor-driven track type traveling system applied into high clearance working machineries in orchard is proposed. Tractive performance and hill climbing ability were predicted and evaluated for the optimal motor traveling system by taking into account of soil characteristics in orchard utilizing the high clearance working machineries. Design criteria for tractive performance were based on the traction force calculated from tractive effort subtracted by motion resistance, while hill climbing ability had its design criteria that fulfill the climbing 20% slope ground at a speed of 3km/h. Based on the evaluation results of traction and climbing ability, two DC48V, 4500rpm, 1.6kW AC motors were independently applied to both left and right side of orbits; each motor is designed to transmit power on driving sprocket of track type traveling system via 50:1 reduction gear ratio. The motor-driven track type traveling system developed in the study found to have 396 kgf of tractive force, which is 12.5% higher than climbing resistance at orchard soil having 20% slope ground (352 kgf), demonstrating sufficient tractive performance and hill climbing ability.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.41 no.8
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• pp.771-782
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• 2017

This paper describes the development of a wearable robot to assist ankle power for the elderly. Previously developed wearable robots have generally used motors and gears to assist muscle power during walking. However, the combination of motor and reduction gear is heavy and has limitations on the simultaneous control of stiffness and torque due to the friction of the gear reducer unlike human muscles. Therefore, in this study, Mckibben pneumatic muscle, which is lighter, safer, and more powerful than an electric motor with gear, was used to assist ankle joint. Antagonistic actuation using a pair of pneumatic muscles assisted the power of the soleus muscles and tibialis anterior muscles used for the pitching motion of the ankle joint, and the model parameters of the antagonistic actuator were experimentally derived using a muscle test platform. To recognize the wearer's walking intention, foot load and ankle torque were calculated by measuring the pressure and the center of pressure of the foot using force and linear displacement sensors, and the stiffness and the torque of the pneumatic muscle joint were then controlled by the calculated ankle torque and foot load. Finally, the performance of the developed ankle power assistive robot was experimentally verified by measuring EMG signals during walking experiments on a treadmill.

• Journal of the Korean Society for Nondestructive Testing
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• v.34 no.2
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• pp.165-170
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• 2014

Shear horizontal (SH) waves propagate as a type of plate wave in a thin sheet. The dispersion characteristics of SH waves can be used for signal analysis. Therefore, SH-waves are useful for monitoring the structural health of a thin-sheet-structure. An electromagnetic acoustic transducer (EMAT), which is a non-contact ultrasonic transducer, can generate SH-waves easily by varying the shape and array of magnets and coils. Therefore, an EMAT can be applied to an automated ultrasonic testing system for structural health monitoring. When used as a sensor, however, the EMAT has a weakness in that electromagnetic interference (EMI) noise can occur easily in the automated system because of motors and electric devices. Alternatively, a fiber optic sensor works well in the same environment with EMI noise because it uses a light signal instead of an electric signal. In this paper, a fiber Fabry-P$\acute{e}$rot interferometer (FFPI) was proposed as a sensor to receive the SH-waves generated by an EMAT. A simple test was performed to verify the performance of the FFPI sensor. It is thus shown that the FFPI can receive SH-wave signals clearly.

• Resources Recycling
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• v.25 no.6
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• pp.13-22
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• 2016

This study is aimed at compare and evaluate the environmental impact of End-of-Life Vehicle(ELV) on the eco-friendly technology dismantling and recycling system, using Life Cycle Assessment (LCA) method. In this study, it was analyzed for the environmental impacts of raw materials, disassemble process, recycle parts separation and waste treatment into the process of ELV treatment by greenhouse gas and resource consumption, etc. Through this study, the indoor rail type dismantling technology were recycling rate applied on the alternate system was increased by approximately 8%. As a result, it was 3 to 88% by improving the environmental impact category. In addition, the added benefit of approximately 8 - 62% in pre-market occurred through the recycling rate, improve parts reuse rate of ELV. Through the results of this study, legal compliance, improved reuse and recycling ratio, used parts market reach, enable exports has identified the need for the effort that the dissemination and diffusion of eco-friendly technology.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.10
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• pp.415-424
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• 2019

In this paper, a minimum rotation radius was designed and fabricated to overcome the threshold so that elderly or disabled people who have difficulty moving can move and transfer safely and conveniently in a narrow room. In the indoor environment, where the sedentary culture develops, this study aimed to provide convenience for passengers with fracture diseases, geriatric diseases, and other knee and waist diseases. First, links, seats, armrests, covers, motors, batteries, chargers, controllers, etc. were attached to the frame so that they could be moved and lifted indoors. The product design and structure were designed considering the user's environment and physical characteristics, and IoT functions were added. A driving experiment was performed to confirm the operating performance of the manufactured indoor moving and lifting wheelchair. The performance tests, such as continuous running time, turning radius, maximum actuator load, maximum lift height, sound pressure level, minimum sensing distance of the driving aid sensor, interworking of server and app programs, device compatibility, and duty cycle error rate, were performed. As a result of the test, the built-in wheelchair could achieve the performance test target of each item and operate successfully.

• Journal of the Korean Association of Geographic Information Studies
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• v.24 no.3
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• pp.58-72
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• 2021

In July 2021, UNCTAD classified Korea as a developed country. After the Korean War in the 1950s, economic development was promoted despite difficult conditions, resulting in epoch-making national growth. However, in order to respond to the rapidly changing global economy, it is necessary to continuously study the domestic industrial ecosystem and prepare strategies for continuous change and growth. This study analyzed the industrial ecosystem of the automobile industry where it is possible to obtain transaction data between companies by applying complexity spatial network analysis. For data, 295 corporate data(node data) and 607 transaction data (link data) were used. As a result of checking the spatial distribution by geocoding the address of the company, the automobile industry-related companies were concentrated in the Seoul metropolitan area and the Southeastern(Dongnam) region. The node importance was measured through degree centrality, betweenness centrality, closeness centrality, and eigenvector centrality, and the network structure was confirmed by identifying density, distance, community detection, and assortativity and disassortivity. As a result, among the automakers, Hyundai Motor, Kia Motors, and GM Korea were included in the top 15 in 4 indicators of node centrality. In terms of company location, companies located in the Seoul metropolitan area were included in the top 15. In terms of company size, most of the large companies with more than 1,000 employees were included in the top 15 for degree centrality and betweenness centrality. Regarding closeness centrality and eigenvector centrality, most of the companies with 500 or less employees were included in the top 15, except for automakers. In the structure of the network, the density was 0.01390522 and the average distance was 3.422481. As a result of community detection using the fast greedy algorithm, 11 communities were finally derived.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.12 no.2
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• pp.162-169
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• 2019

Non-destructive freshness measurement using spectroscopy has been carried out several times, but research on freshness and freshness has not been conducted. Therefore the purpose of this study is to develop a system for visually measuring and quantifying the air sack inside the egg by non - destructive method. The experimental environment which designed a small chamber was composed of 850nm band of two IR lasers, IR camera and two servo motors to acquire air sack Images. When the air sack volume ratio is 2.9% or less and the density is 0.9800 or more, the Haugh Unit value is 60 or more It was judged to be a fresh egg of a grade B or higher. These results mean, using the weight measurement, nondestructive decision system, and freshness evaluating algorithm. It can be expected to distinguish grade B or more marketable eggs without using destructive methods.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.3
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• pp.455-464
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• 2019

The sensorless control based on the flux linkage of PM synchronous motors has excellent position estimation characteristics at low speeds. However, a limitation arises because the integrator of flux estimator is saturated by the DC offset generated during the analog to digital conversion(ADC) process of the measured current. In order to overcome this limitation, HPF with a low cutoff frequency is used. However, the estimation performance is deteriorated (Ed- the verb deteriorate already includes the meaning of 'problem') at high speed due to the low cutoff frequency, and increasing the cutoff frequency of the HPF induces further problems of phase leading and initial starting failure at low speeds. In this paper, the cutoff frequency of HPF was synchronized to the operation frequency of the motor: at low speeds the cutoff frequency was set to low in order to reduce the phase leading of the estimated flux, and at high speeds it was set to high to raise the DC offset removal performance. As a result, the operating range was increased by 200%. Furthermore, a phase compensation algorithm is proposed to reduce the phase leading of the HPF to less than 1.5 degrees over the full operating range. The proposed sensorless control algorithm was verified by experiment with a PM synchronous motor for a washing machine.