• 한국동물위생학회지
• /
• 제46권2호
• /
• pp.115-122
• /
• 2023

This study was performed to investigate the distribution of mosquito vectors related to the zoonotic disease in Daejeon. Samples were taken using a blacklight trap once a month from March to November 2021 at the slaughterhouse in Daejeon. A total of 820 mosquitoes were captured and classified into 5 genera and 8 species. Among the collected mosquitoes, 319 (38.9%) and 295 (35.93%) were Aedes vexans nipponii and Culex pipiens pallens, respectively, making them the dominant species. The overall number of mosquitoes collected started to increase from May and reached the largest value of 329 (40.12%) in June. Trapped mosquitoes are created 72 pools by environmental condition and by species. The pools were tested by PCR methods for 7 zoonotic pathogens. Flavivirus-positive products were confirmed by DNA sequencing. Japanese encephalitis viruses were detected in 3 pools collected from cow lairage (Culex pipiens pallens) in May, cow by-product processing room (Aedes vexans nipponii) in June and cow lairage (Mansonia uniformis) in June. Culex flavivirus were detected in 4 pools. Based on the results of this study, it is considered that continous surveillence of mosquitoes in livestock assembly facilities (slaughterhouse) should be performed for controlling mosquito populations and mediating disease spread by mosquitoes.

• Journal of Microbiology and Biotechnology
• /
• 제30권11호
• /
• pp.1651-1658
• /
• 2020

Since Zika virus (ZIKV) was first detected in Uganda in 1947, serious outbreaks have occurred globally in Yap Island, French Polynesia and Brazil. Even though the number of infections and spread of ZIKV have risen sharply, the pathogenesis and replication mechanisms of ZIKV have not been well studied. ZIKV, a recently highlighted Flavivirus, is a mosquito-borne emerging virus causing microcephaly and the Guillain-Barre syndrome in fetuses and adults, respectively. ZIKV polyprotein consists of three structural proteins named C, prM and E and seven nonstructural proteins named NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5 in an 11-kb single-stranded positive sense RNA genome. The function of individual ZIKV genes on the host innate immune response has barely been studied. In this study, we investigated the modulations of the NF-κB promoter activity induced by the MDA5/RIG-I signaling pathway. According to our results, two nonstructural proteins, NS2A and NS4A, dramatically suppressed the NF-κB promoter activity by inhibiting signaling factors involved in the MDA5/RIG-I signaling pathway. Interestingly, NS2A suppressed all components of MDA5/RIG-I signaling pathway, but NS4A inhibited most signaling molecules, except IKKε and IRF3-5D. In addition, both NS2A and NS4A downregulated MDA5-induced NF-κB promoter activity in a dosedependent manner. Taken together, our results suggest that NS2A and NS4A signifcantly antagonize MDA5/RIG-I-mediated NF-κB production, and these proteins seem to be controlled by different mechanisms. This study could help understand the mechanisms of how ZIKV controls innate immune responses and may also assist in the development of ZIKV-specific therapeutics.