• 제목/요약/키워드: Morphometric study

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Morphological and molecular characterization of root-lesion nematode Pratylenchus hippeastri from Korea

  • Sungchan Huh;Namsook Park;Heonil Kang;Changhwan Bae;Insoo Choi
    • Korean Journal of Environmental Biology
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    • 제41권4호
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    • pp.657-665
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    • 2023
  • The root-lesion nematode Pratylenchus spp. is the most important plantparasitic nematode due to its worldwide distribution, wide host ranges, and migratory endoparasitic characteristics. One population of Pratylenchus collected from the giant pussy willow (Salix chaenomeloides Kimura) in the Andong area as part of a nematode survey in Korea was characterized morphologically and by molecular methods. The analysis of morphological measurements and morphometric characteristics, as well as DNA sequencing of the rRNA large subunit (LSU) D2/D3 expansion segments and the internal transcribed spacer (ITS) gene sequence, confirmed the identity of this population as P. hippeastri. This study is the first report of P. hippeastri associated with Salix chaenomeloides in Korea and worldwide. Further studies on distribution and pathogenicity in different P. hippeastri host crops, such as grapevines, strawberries, and apples, are necessary. The taxonomic keys to 16 Pratylenchus species in Korea are provided.

Atlantooccipital assimilation associated with combined atlas arch defect: a radiological case report

  • Athikhun Suwannakhan;Pannawat Trerattanavong;Laphatrada Yurasakpong;Woranan Kirisattayakul;Nutmethee Kruepunga;Kiarttiyot Tuntiseranee;Kanitin Rumpansuwon;Thanyaporn Senarai
    • Anatomy and Cell Biology
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    • 제57권3호
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    • pp.468-472
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    • 2024
  • In this report, atlantooccipital assimilation (AS), anterior arch defect (AAD), and posterior arch defect (PAD) of the atlas, and several variations around the craniocervical junction were identified on computed tomography (CT) of a patient of unknown sex and age. Coronal and sagittal CT scans showed AS and bilateral fusion of the atlas and the base of occipital bone. Axial CT scan at the atlas revealed PAD type B on the left side and midline AAD. Morphometric measurements indicated a potential ventral spinal cord compression. In addition, mid-sagittal CT revealed the presence of fossa navicularis magna and incomplete formation of the transverse foramen on the right side. This study reports an extremely rare AS associated with AAD, PAD, and other variations of the clivus and the atlas. To our knowledge, no similar case has been reported in the literature.

In Vitro Cultivation of Cymatocarpus solearis(Brachycoeliidae) Metacercariae to Obtain the Adult Stage without the Marine Turtle Definitive Host

  • Grano-Maldonado, Mayra;Alvarez-Cadena, Jose
    • Parasites, Hosts and Diseases
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    • 제48권1호
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    • pp.49-55
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    • 2010
  • In vitro cultivation of trematodes would assist studies on the basic biology of the parasites and their hosts. This is the first study to use the yolk of unfertilized chicken eggs as a simple and successful method of ovocultivation and the first time to obtain the adult-stage of the trematode Cymatocarpus solearis Braun, 1899 (Digenea: Brachycoeliidae). Chicken eggs were inoculated with metacercariae from the muscle of the spiny lobster, Panulirus argus (Latreille, 1804). The metacercariae were excysted and incubated for 576 hr (24 days) at $38^{\circ}C$ to obtain the adult stage. Eggs in utero were normal in shape and light brown color. The metacercariae developed into mature parasites that have been identified as the adultstage found in marine turtles. The adult lobsters collected in Quintana Roo State, Mexico, showed the prevalence of 49.4% and the mean intensity of 26.0 per host (n=87). A statistical study was performed to determine that no parasitic preference was detected for male versus female parasitized lobsters. Morphometric measurements of the adult-stage of C. solearis obtained in our study have been deposited in the National Helminths Collection of the Institute of Biology of the National Autonomous University of Mexico. This study is significant because it is the first time that a digenean of the family Brachycoeliidae has been demonstrated to develop in vitro from metacercariae into adults capable of producing eggs using the yolk of unfertilized chicken eggs. Secondly, this technique allows to obtain the adult stage of C. solearis without the presence of its marine turtle host, allows us to describe the mature parasites, and thus contribute to our understanding of the biology of C. solearis.

Morphometric analysis of bone in the ovariectomized rat using in vivo micro-CT (In vivo micro-CT를 이용한 난소적출 백서의 골구조 형태계측학적 분석)

  • Lee, Chang-Jin;Lee, Wan;Lee, Byung-Do
    • Imaging Science in Dentistry
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    • 제38권1호
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    • pp.29-37
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    • 2008
  • Purpose: The aim of this study was to observe the bony change in the OVX rat longitudinally and to study the alendronate effect. Materials and Methods: Eighteen Sprague-Dewley rats, eight-week old each, were randomly assigned into three groups: one of those sham-operated (N=4), the other two were OVX: saline-treated (N=7) and alendronate-treated group (N=7). The saline-treated group was administered with saline solution (0.1mL/100g) daily, while the alendronate-treated group was given alendronate (1mg/kg, Sigma-Aldrich Corp. Korea) daily. Micro-CT scannings of the lumbar were consecutively done at baseline, at 3-week intervals during 9 weeks. Two and three dimensional bony analysis were done. Bone mineral density (BMD) was measured with Piximus (GE Lunar Co. USA). The average values of these three methods were compared with each group. Results: After 6 weeks the BMD of the OVX group showed lower tendency than that of sham group. After 6 weeks many 3D parameters of micro-CT showed higher values in the OVX-alendronate group compared with the OVXsaline group. Most 2D bony parameters were higher in the OVX-alendronate group compared with the OVX-saline group at 9 weeks. Conclusion: This study showed low BMD of the OVX group after 6 weeks and showed the effect of alendronate on the BMD and bony structures of ovariectomized rats. This study also showed usefulness of in vivo micro-CT in monitoring individual bone changes over time.

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Morphometric analysis of sacral corridor in the upper three sacral segments to prevent neurovascular injury

  • Binita Chaudhary;Prem Kumar;Ruchika Narayan;Adil Asghar;Padamjeet Panchal;Neelam Kumari
    • Anatomy and Cell Biology
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    • 제57권2호
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    • pp.221-228
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    • 2024
  • Although studies of the sacral corridor dimension have been reported in the European population, little attention has been paid to this issue in the Asian population. The purpose of the study is to estimate the safe dimension of the corridor to avoid neurovascular damage during the fixation of the sacral fracture. The study aimed to examine the cephalocaudal (vertical) and the anteroposterior diameter of the bony passage in the upper three sacral segments. The study further examines the effect of age and sex on corridor dimensions at different sacral levels. Three-dimensionally reconstructed sacra from computed tomography of normal subjects were included in the study. Cephalocaudal and anteroposterior diameters were measured in coronal and axial sections using Geomagic Freeform Plus software. Anteroposterior diameter of the sacral corridor at the first, second, and third sacral segments are significantly higher in males (P=0.013, 0.0011, and <0.0001, respectively). The length of the sacrum also revealed sexual dimorphism (P<0.00016). The anteroposterior diameter of the second sacral segment (ap-S2c) correlated moderately with the first sacral anteroposterior diameter (ap-S1c) (R=0.519, P<0.001). The ap-S2c exhibited a moderate correlation to the third sacral segment (ap-S3c) (R=0.677, P<0.001). The sacral corridor at the level of S1 has the largest cephalocaudal (18.25 mm) and anteroposterior diameter (17.11 mm). Placement of the screw in the first sacral corridor may avoid damage to the neurovascular bundle during the fixation of the sacral fracture.

Morphometric Relationship between the Cervicothoracic Cord Segments and Vertebral Bodies

  • Kim, Ji Hoon;Lee, Chul Woo;Chun, Kwon Soo;Shin, Won Han;Bae, Hack-Gun;Chang, Jae Chil
    • Journal of Korean Neurosurgical Society
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    • 제52권4호
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    • pp.384-390
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    • 2012
  • Objective : The objective of this study was to investigate the morphologic characteristics between the vertebral body and the regions of the cervical and thoracic spinal cords where each rootlets branch out. Methods : Sixteen adult cadavers (12 males and 4 females) with a mean age of 57.9 (range of 33 to 70 years old) were used in this study. The anatomical relationship between the exit points of the nerve roots from the posterior root entry zone at each spinal cord segment and their corresponding relevant vertebral bodies were also analyzed. Results : Vertical span of the posterior root entry zone between the upper and lower rootlet originating from each spinal segment ranged from 10-12 mm. The lengths of the rootlets from their point of origin at the spinal cord to their entrance into the intervertebral foramen were 5.9 mm at the third cervical nerve root and increased to 14.5 mm at the eighth cervical nerve root. At the lower segments of the nerve roots (T3 to T12), the posterior root entry zone of the relevant nerve roots had a corresponding anatomical relationship with the vertebral body that is two segments above. The posterior root entry zones of the sixth (94%) and seventh (81%) cervical nerve roots were located at a vertebral body a segment above from relevant segment. Conclusion : Through these investigations, a more accurate diagnosis, the establishment of a better therapeutic plan, and a decrease in surgical complications can be expected when pathologic lesions occur in the spinal cord or vertebral body.

Clinical Outcome of Modified Cervical Lateral Mass Screw Fixation Technique

  • Kim, Seong-Hwan;Seo, Won-Deog;Kim, Ki-Hong;Yeo, Hyung-Tae;Choi, Gi-Hwan;Kim, Dae-Hyun
    • Journal of Korean Neurosurgical Society
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    • 제52권2호
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    • pp.114-119
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    • 2012
  • Objective : The purpose of this study was 1) to analyze clinically-executed cervical lateral mass screw fixation by the Kim's technique as suggested in the previous morphometric and cadaveric study and 2) to examine various complications and bicortical purchase that are important for b-one fusion. Methods : A retrospective study was done on the charts, operative records, radiographs, and clinical follow up of thirty-nine patients. One hundred and seventy-eight lateral mass screws were analyzed. The spinal nerve injury, violation of the facet joint, vertebral artery injury, and the bicortical purchases were examined at each lateral mass. Results : All thirty-nine patients received instrumentations with poly axial screws and rod systems, in which one hundred and seventy-eight screws in total. No vertebral artery injury or nerve root injury were observed. Sixteen facet joint violations were observed (9.0%). Bicortical purchases were achieved on one hundred and fifty-six (87.6%). Bone fusion was achieved in all patients. Conclusion : The advantages of the Kim's technique are that it is performed by using given anatomical structures and that the complication rate is as low as those of other known techniques. The Kim's technique can be performed easily and safely without fluoroscopic assistance for the treatment of many cervical diseases.

A morphometric study on stainless steel crowns of the primary first molar using a three dimensional scanner

  • Lee, Jihyun;Shin, Teo Jeon;Kim, Young-Jae;Kim, Jung-Wook;Jang, Ki-Taeg;Lee, Sang-Hoon;Kim, Chong-Chul;Hyun, Hong-Keun
    • The Journal of the Korean dental association
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    • 제54권6호
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    • pp.414-428
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    • 2016
  • Objectives: The aim of this study was to assess the morphologic characteristics of two types of stainless steel crowns (SSCs) for the first primary molar using a 3D scanner. Study design: Two types of SSCs, KIDS CROWN (KC) and 3M ESPE ND-96 (ND), for the first primary molars were scanned using a 3D scanner. The mesiodistal and buccolingual diameters at the height of the contour and the cervical margin, occlusocervical diameters on the mesial, distal, buccal, and lingual aspects were measured, and the crown shape ratio, the smooth surface crown height ratio, and the cervical convergence were calculated. Results: In the crown shape ratio of the mandibular SSC, KC was larger buccolingually compared with ND. In the smooth surface crown height ratio, ND was larger than KC in all of the maxilla and mesial, distal, and lingual aspects of the mandible. ND was more convergent to the cervical mesiodistally and buccolingually compared with KC. Conclusion: In the superimposed images of the maxillary SSC, the mesiolingual and distolingual line angles of KC were more prominent compared with ND. In the mandible, ND demonstrated higher cusps and more obvious buccal developmental lobes than KC. ND showed a larger cervical undercut than KC.

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Characteristics of Channel Bend Reach and Shape of Cross-Section (유로 만곡부 특성과 단면현상)

  • Song, Jai Woo;Park, Young Jin;Lee, Yong Hwan
    • KSCE Journal of Civil and Environmental Engineering Research
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    • 제14권5호
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    • pp.1191-1197
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    • 1994
  • The purpose of this study is to examine morphometric characteristics in a channel bend reach. The new shape factor is suggested that channel deformation rate of cross section (${\Delta}A_s$) showed the variation of concentrated location of force due to the current and the variation of erosional section in alluvial channel. In the downstream direction the meaning of decreasing "${\Delta}A_s$" is the stability of channel bed. This study was analyzed morphological characteristics of cross section-width of channel ($W_s$), width to the thalweg ($W_{th}$), maximum depth ($D_{th}$)-on the Guem River, and typical cross sections in channel bend were proposed. The channel migration rate (M) for the study river was represented that the zones of curvature ratio (R/W) with 2~4 were larger 12% than other zones.

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Comparative Analysis of Cervical Lateral Mass Screw Insertion among Three Techniques in the Korean Population by Quantitative Measurements with Reformatted 2D CT Scan Images : Clinical Research

  • Cho, Jae-Ik;Kim, Dae-Hyun
    • Journal of Korean Neurosurgical Society
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    • 제44권3호
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    • pp.124-130
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    • 2008
  • Objective : Our purpose of this study is to compare insertion angles and screw lengths from Roy-Camille, Magerl, and our designed method for cervical lateral mass screw fixation in the Korean population by quantitative measurement of reformatted two dimensional (2D) computed tomography (CT) images. Methods : We selected thirty Korean patients who were evaluated with thin section CT scans and reconstruction program to obtain reformatted 2D-CT images of the transversal plane passing the cranio-caudal angle using three different techniques. We measured the minimum angle to avoid vertebral artery (VA) injury, the ideal angle and depth for bicortical screwing of cervical lateral mass. Morphometric measurements of the lateral masses from C3-C7 were also taken. Results : In all three techniques, the mean safety angles from the VA were less than 8 degrees and the necessary depth of the screw was about 14 mm for safety to the VA and for the bicortical purchase. In our designed technique, the mean $\beta$ angles of each level from C3 to C7 were 29.0. 29.8. 29.5. 26.3. and 23.9 degrees, respectively. Conclusion : Results of this study and data from the literature indicate that differences may exist between the Korean and Western people in the length and angle for ideal lateral mass screw fixation. In addition, our technique needs further cadaveric and clinical study for safety and efficacy for being performed as alternative method for cervical lateral mass fixation.