• Korean Journal of Fisheries and Aquatic Sciences
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• v.36 no.6
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• pp.632-640
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• 2003

Geographic variation in shell morphology of Thais clavigera $(K\"{u}ster)$ (Gastropoda: Muricidae) was investigated using samples collected from 24 sites along the Korean coast. Multivariate statistical analysis was applied to 9 morphometric and 4 categorical variables. The shells of T. clavigera were classified into two distinct morph types (Type-W and -E). Temperature and salinity of the sampling sites were significantly correlated with the incidence of morph types. Relative abundance of Type-W (thin, yellowish brown shell with triangular nodules) was positively correlated with temperature and negatively correlated with salinity. In contrast, relative abundance of Type-E (thick, dark purple shell with round nodules) was negatively correlated with temperature and positively correlated with salinity. Possible correlation between environmental factors (temperature and salinity) and morphological variations in the shells were discussed.

• Development and Reproduction
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• v.16 no.1
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• pp.47-51
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• 2012

Genomic DNAs (gDNAs) were isolated from the hard clam ($Meretrix$ $lusoria$, Roding, 1798) populations of Gunsan located in the Yellow Sea of the Korean peninsula. Genetic distances among different individuals of the LSCP (light shell color population) population of the hard clam (lane 1-11), GSCP (grey shell color population) population of the hard clam (lane 12-22) and DSCP (dark shell color population) population of the hard clam (lane 23-33), respectively, were generated using Systat version 10 according to the bandsharing values and similarity matrix. The dendrogram, generated by seven reliable oligonucleotides primers, indicates 3 genetic clusters. LSCP population could be evidently discriminated with the other two populations among three populations. The longest genetic distance (0.801) was found to exist between individuals in the two populations, between individuals' no. 33 of the DSCP population and no. 06 of the LSCP population. The higher fragment sizes (>2,000 bp) are much more observed in the GSCP population. Three hard clam populations can be clearly distinguished, especially, by their morphological characters and PCR-based approach.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.7 no.2
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• pp.87-90
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• 1974

The followings are the results of study about morphological variation of mussel, Mytilus coruscus collected respectively from Sanyang-myeon Tongyeong-goon Gyeongnam on 13th January, Yeonwha-do Yokji-myeon Tongyeong-goon Gyeongnam on 15th May and down below the Yeong-do Bridge Busan on 20th August, 1964. The biggest mussel of the above three areas have been from Yeong-do Bridge, whose shell height is 164.1 mm, shell length 77.8mm, shell breadth 52.2mm, total weight 291,9g and shell length is as follows : from Yeong-do Bridge L=0.4954H + 1.9516, from Sanyang-myeon L=0.3718H + 14.145, from Yeonwha-do L=0.4074H + 9,6610 The relationship between shell height and shell breadth is as follows : from Yeong-do Bridge B=0.3426H + 0.2052, from Sanyang-myeon B=0.3084H + 3.6183, from Yeonwha-do B=0.3507H + 0.8028 In view of the above relationship, it is concluded that the slope value of mussel, nearer to the inshore from the off-sea, is similar to that of M. edulis, from which are can presume that M. coruscus could be changed in shell form according to its environment. Growth curve between total weight and shell height is as follows : from Yeong-do Bridge W=0. 00020469 $$H^{2.79745}$ from Sanyang-myeon W=0.00061512$H^{2.53708}$ from Yeonwha-do W=0.00016965$H^{2.83960}$

• Applied Chemistry for Engineering
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• v.31 no.4
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• pp.390-397
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• 2020

To synthesize non-corrosive metallic gold colored α-Al₂O₃ nanoplate-TiO₂ core-shell pigments with controlled roughness, we systematically checked the morphological variation of the TiO₂ shell with the mole ratio of TiCl₄ and NaOH from 1 : 1 to 1 : 1.5, 1 : 2, 1 : 2.5, 1 : 3, 1 : 3.5, and 1 : 4. The more increased mole ratio of TiCl₄ and NaOH resulted in the smoother TiO₂ shell due to the promoted formation of anatase TiO₂ than that of the rutile one. By the heat-treatment of pigments at 500 ℃, we could improve the adhesiveness between TiO₂ shell and α-Al₂O₃ nanoplates without changing their topology and roughness. In addition, the α-Al₂O₃ nanoplate with the robust TiO₂ by heat-treatment exhibited comparable photo-stability against photo-catalytic degradation by UV exposure compared with the commercially available α-Al₂O₃/TiO₂ lustering pigment.

• Animal Systematics, Evolution and Diversity
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• v.27 no.1
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• pp.1-17
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• 2011

Thais Roding, 1798, commonly known as rock-shell, is among the most frequently found gastropod genera worldwide on intertidal rocky shores including those of Japan, China, Taiwan and Korea. This group contains important species in many marine environmental studies but species-level taxonomy of the group is quite complicated due to the morphological variations in shell characters. This study examined the genetic variations and relationships among three Korean Thais species based on the partial nucleotide sequences of mitochondrial cox1 gene fragments. Phylogenetic trees from different analytic methods (maximum parsimony, neighbor-joining, and maximum likelihood) showed that T. bronni and T. luteostoma are closely related, indicating the most recent common ancestry. The low sequence divergence found between T. luteostoma and T. bronni, ranging from 1.53% to 3.19%, also corroborates this idea. Further molecular survey using different molecular marker is required to fully understand a detailed picture of the origin for their low level of interspecific sequence divergence. Sequence comparisons among conspecific individuals revealed extensive sequence variations within the three species with maximum values of 2.43% in T. clavigera and 1.37% in both T. bronni and T. luteostoma. In addition, there is an unexpectedly high level of mitochondrial genotypic diversity within each of the three Korean Thais species. The high genetic diversity revealed in Korean Thais species is likely to reflect genetic diversity introduced from potential source populations with diverse geographic origins, such as Taiwan, Hong Kong, and a variety of different coastal regions in South China and Japan. Additional sequence analysis with comprehensive taxon sampling from unstudied potential source populations will be also needed to address the origin and key factors for the high level of genetic diversity discovered within the three Korean Thais species studied.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.6 no.1_2
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• pp.65-75
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• 1973

Morphological variations of the Korean Oyster, Crassostrea gigas, were studied on the basis of the oyster samples collected from seven ecologically different areas in May and October of 1972 respectively. The sampling areas were Daecheon and Buan in the west coast, Namseong, Cha-myeon, Imyeong and Chubong in the south coast and Pohang in the east coast. The average sizes of the oysters of the west coast were smaller than those of the south coast as well as those of the east coast. The oysters of the east were largest in size, The oysters from subtidal habitats (Chubong and Pohang) had elongated shells and those from intertidal habitats (Buan, Namseong, Imyeong and Chamyeon) had slanted shells. The samples from subtidal habitats showed size variations depending on cultural methods applied. The oysters from Chubong reared by hanging method had their shells as compared to those from Pohang reared by bottom culture method. The ratio of shell height to shell length showed a great variation froms areas to areas with a significant differences. The fatness (meat weight / shell weight) of all the samples collected in October revealed higher value than those collected in May. The oysters from Chubong showed maximum fatness, and those of Pohang showed minimum fatness. Seasonal variations in fatness was not significant in Chubong. On the other hand, it was relatively significant in the oysters from Pohang, Chamyeon, Imyeong and Buan. Water content of the oyster meat was greater in October than in May. It also showed variations in the sampling areas with higher values in the oyster from Imyeong and Chamyeon than those from Chubong, Pohang and Daecheon.

• Fisheries and Aquatic Sciences
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• v.2 no.1
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• pp.98-104
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• 1999

The characteristics of the populations of shortnecked clam (Ruditapes philipinarum) originated from three different seed-production sites, Hadong, Kochang and Ulsan along the coast of Korea, were analysed by the morphological differences and the random amplified polymorphic DNA (RAPD) profiles. The morphology of the shell and survival rate for each population were also investigated 13-months after transplantation to a farming site in Shinsung. The morphology of the populations from Hadong and Kochang showed significant differences (P<0.05), but one year after transplantation to Shinsung, the morphology of these three populations was no longer significantly different (P>0.05). The template DNA for RAPD was efficiently extracted from the digestive diverticula of the clams. Up to 13 of amplified fragments were detected using arbitrary primers. Within the species of R. philipinarum, the genetic similarities ranged from 0.196 to 0.259. The populations from Hadong and Ulsan showed the highest similarity. The survival rates of the populations from Hadong $(69.4\%)$ and Ulsan $(63.8\%)$ were higher than that from Kochang $(41.7\%)$ 13-months after transplantation. From the RAPD analysis, it could be used as one of the primary criterion in determining which shellfish populations among various seed-production sites tend to be genetically similar and more adaptable and transplantable to a farming site.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.33 no.6
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• pp.533-540
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• 2000

Gonadal development, gametogenesis, reproductive cycle, gonad index, flesh weight rate, and first sexual maturity of the turban shell, Lunella coronata coreensis were investigated by histological observation. The materials used were collected monthly from the rocky intertidal zone of Daehang-ri, Buan-gun, Jeollabuk-do, on the west coast of Korea, from July 1998 to June 1999. Sex of L coronata coreensis was separate. The gonad was widely located in the spirals of the visceral mass buried in the digestive gland. The ovary and testis were composed of a number of oogenic follicles and speymatogenic follicles, respectively. Monthly variations in the gonad index increased from March ($23.86{\pm}3.73$) when the water temperature increased and reached the maximun in July ($49.76{\pm}6.47$). And then, the gonad index sharply decreased in September ($15.58{\pm}2.33$). The flesh weight rate ranged from $25.2{\%}$ to $32.3{\%}$, and its variation showed a similar pattern to the gonad index. Individuals $<5.9 mm$ in shell height could not take part in reproduction in both sexes. Percentages of first sexual maturity of female and male specimens ranging from $7.0{\~}7.9 mm$ in shell heights were $84.6{\%}\;and\;91.7{\%}$, respectively, and $100{\%}$ in those over 8.0 mm in shell height in both sexes took part in reproduction. By studying the monthly changes of the morphological features and sizes of germ cells during gametogenesis in the gonad, the reproductive cycle of this species could be devided into five successive stages: early active (December to April), late active (January to July), ripe (May to August), spawning (July to September), and recovery (September to March). The spawning period of this species was once a year between July and September, and the main spawning occurred in July when the seawater temperature reached above $24.8^{\circ}C$. The fully ripe eggs were $150{\~}160\;{\mu}m$ in diameter.

• Development and Reproduction
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• v.10 no.4
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• pp.227-238
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• 2006

Genomic DNAs(gDNAs) were isolated from the venus clam(Gomphina aequilatera) from Samcheok(venus clam from Samcheok; VCS) and Wonsan(venus clam from Wonsan; VCW) located in the East Sea of the Korean Peninsula. The amplified products were generated by agarose gel electrophoresis(AGE) with oligonucleotides primer, detected by staining with ethidium bromide and viewed by ultraviolet ray. The seven arbitrarily selected primers BION-21, BION-23, BION-25, BION-27, BION-29, BION-31 and BION-33 generated the shared loci, polymorphic, and specific loci, with the molecular sizes ranging from 150 bp to 2,400 bp. In this study, 147 polymorphic loci(147/954 loci, 15.41%) in VCS population and 274(274/996 loci, 27.51%) in VCW population were generated with seven primers. These results suggest the genetic variation in VCW population is higher than in VCS population. Especially, the 700 bp bands generated by the primer BION-21 were identified commonly in two Gomphina populations, which identified populations and/or species. This specific primer was found to be useful in the identification of individuals and/or population, resulting from the different DNA polymorphism among individuals/species/population. Two Gomphina populations between the individual SAMCHEOK no. 03 and WONSAN no. 22 showed the longest genetic distance(0.696) in comparison with other individuals used. The complete linkage cluster analysis indicating three genetic groupings and dendrogram revealed close relationships among individual identities within two geographical populations of venus clam(G. aequilatera) from the Samcheok and Wonsan. The intra-species classification and clustering analyses inferred from molecular markers supported the traditional taxonomy of the species based on morphological characters such as shell size, shape and color. Accordingly, as mentioned above, RAPD analysis showed that VCS population was more or less separated from VCW population.