• Molecules and Cells
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• 제25권2호
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• pp.163-171
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• 2008

The genus Cynodon comprises ten species. The objective of this study was to evaluate the genetic diversity of Korean bermudagrasses at the morphological, cytological and molecular levels. Morphological parameters, the nuclear DNA content and ploidy levels were observed in 43 bermudagrass ecotypes. AFLP markers were evaluated to define the genetic diversity, and chromosome counts were made to confirm the inferred cytotypes. Nuclear DNA contents were in the ranges 1.42-1.56, 1.94-2.19, 2.54, and 2.77-2.85 pg/2C for the triploid, tetraploid, pentaploid, and hexaploid accessions, respectively. The inferred cytotypes were triploid (2n = 3x = 27), tetraploid (2n = 4x = 36), pentaploid (2n = 5x = 45), and hexaploid (2n = 6x = 54), but the majority of the collections were tetraploid (81%). Mitotic chromosome counts verified the corresponding ploidy levels. The fast growing fine-textured ecotypes had lower ploidy levels, while the pentaploids and hexaploids were coarse types. The genetic similarity ranged from 0.42 to 0.94 with an average of 0.64. UPGMA cluster analysis and principle coordinate analysis separated the ecotypes into 6 distinct groups. The genetic similarity suggests natural hybridization between the different cytotypes, which could be useful resources for future breeding and genetic studies.

• The Korean Journal of Ecology
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• 제24권3호
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• pp.149-156
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• 2001

한국산 거머리말속(Zostera, Zosteraceae) 중 포기거머리말(Zostera caespitosa Miki)의 생태 및 분류학적 특성을 파악하기 위하여 생육 환경과 형태적인 특징을 분석하였다. 한반도에 자생하는 포기거머리말읜 생물지리학적인 분포는 동해연안의 덕산항과 대변, 황해 연안의 백령도 두무진과 남해 연안의 진해에서 종의 실체가 확인되었다. 포기거머리말의 생육지는 만과 항구로 수심 2.5∼5.2 m의 사질과 사니질의 퇴적환경에서 생육하였다. 본 종은 같은 생육지에 생육하는 거머리말 군집보다 깊은 수심에서 군집을 형성하였다. 외부 형태적인 특징은 황해 연안의 개체가 동해 연안의 개체보다 크게 신장하였으며, 생식지가 출현한 시기 의 수온은 6.0∼13.7℃로 1월부터 4월초까지 개화하였다. 포기거머리말의 생육지의 수심, 퇴적물의 평균입도와 유기물함량은 개체의 형태적인 형질 변이에 영향을 미치는 것으로 나타났다.

• Ocean and Polar Research
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• 제32권4호
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• pp.369-377
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• 2010

To characterize ecotoxicological responses to a natural estrogen, $17{\beta}$-estradiol, we evaluated the life-history of the parental ($F_0$) and first generation ($F_1$) of the harpacticoid copepod, Tigriopus japonicus sensu lato. We evaluated the survival of nauplii and copepodites, the number of days until the emergence of copepodites and adult males, the sex ratio, brooding success, and the first brooding day of adult females. No significant differences in the survival rate were noted in response to treatments with different concentrations of $17{\beta}$-estradiol. However, $17{\beta}$-estradiol induced developmental delay and skewed the sex ratio toward males. Copepod development was delayed significantly in the 0.1 and $1\;{\mu}g\;l^{-1}$ $17{\beta}$-estradiol treatment groups relative to the control group, with a more pronounced delay in the $F_1$ group. Body length and biomass were significantly smaller in the $17{\beta}$-estradiol treated groups than in the controls. The male emergence of T. japonicus s.l. was very high in the 10 and $30\;{\mu}g\;l^{-1}$ $17{\beta}$-estradiol treatment group. Furthermore, exposure to $17{\beta}$-estradiol resulted in morphological deformities such as shrinking and swelling of the urosome, twisted setae of the caudal rami, setal loss of swimming legs, abnormal segmentation of antennules, and dwarfism.

• Journal of Biosystems Engineering
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• 제42권3호
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• pp.227-233
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• 2017

Purpose: Morphological properties of soybean roots are important indicators of the vigor of the seed, which determines the survival rate of the seedlings grown. The current vigor test for soybean seeds is manual measurement with the human eye. This study describes an application of a machine vision technique for rapid measurement of soybean seed vigor to replace the time-consuming and labor-intensive conventional method. Methods: A CCD camera was used to obtain color images of seeds during germination. Image processing techniques were used to obtain root segmentation. The various morphological parameters, such as primary root length, total root length, total surface area, average diameter, and branching points of roots were calculated from a root skeleton image using a customized pixel-based image processing algorithm. Results: The measurement accuracy of the machine vision system ranged from 92.6% to 98.8%, with accuracies of 96.2% for primary root length and 96.4% for total root length, compared to manual measurement. The correlation coefficient for each measurement was 0.999 with a standard error of prediction of 1.16 mm for primary root length and 0.97 mm for total root length. Conclusions: The developed machine vision system showed good performance for the morphological measurement of soybean roots. This image analysis algorithm, combined with a simple color camera, can be used as an alternative to the conventional seed vigor test method.

• 한국약용작물학회지
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• 제23권5호
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• pp.400-405
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• 2015

Background : As the characterization of medicinal plants is an important aspect of traditional Asian herbal medicine, this study examines the morphological and anatomical characteristics in four fructus form medicinally important plants belonging to the family Apiaceae. Methods and Results : Fruit morphology of four genera was examined using microtome sections and light microscopy. The Anethi Fructus, Anethum graveolens (Siraja) has unique wing-like and membranous lateral ribs that are, approximately $500{\mu}m$ wide. The Coriandri Fructus, Coriandrum sativum (Hoyuja) does not have oil ducts at the dorsal region of the mericarp and differs in the development of the dorsal ribs. The ribs appear upon drying and therefore, consist of primary and secondary ribs. The Foeniculi Fructus, Foeniculum vulgare (Hoehyang) characteristically develops three dominant dorsal ribs and has a strong aromatic fragrance. Finally, the Cnidii Fructus, Torilis japonica (Sasangja) has many oil ducts at the dorsal and carpophores regions and develops many hooked trichomes one the fructus surface. Conclusions : We conclude from this study that each plant has several prominent and distinguishing morphological and anatomical characteristics. Therefore, fruit morphology is very useful for identifying these medicinal plants. In addition, the correct use and herbal name need to be standardized for plants used traditionally in Asian medicine within Korea, China, and Japan.

• 한국지구과학회지
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• 제26권8호
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• pp.836-843
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• 2005

서해 배타적경제수역내 해사채취구역에서 해저지형 변화를 규명하기 위하여 2회에 걸쳐 단일빔음향측심기를 이용한 측심자료를 획득하였다. 교차점 분석에 의한 측심자료의 정확도는 IHO 표준의 2등급에 해당하였다. 지형도는 폭 300 m, 깊이 10 m의 구덩이 형태를 나타내었으며, 이러한 지형의 변화 과정이 두 지형도 수심차의 분포에서 표현되었다. 그러나 해사채취량을 정량화하기 위해서는 더욱 정확하고 정밀한 자료가 요구된다. 이러한 지형변화는 퇴적환경 및 저서생태 환경에 영향을 미칠 수 있으므로 한정된 모래자원을 관리하고 지속가능한 개발을 위해서는 과학적인 조사자료에 근거한 환경영향평가가 요구된다.

• 대한지리학회지
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• 제44권1호
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• pp.31-55
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• 2009

이 연구에서는 한반도 전체의 수치고도모델 분석을 통해 평탄지의 공간적 분포를 살펴보고, 평탄지의 유형을 분류한 뒤, 그 결과를 토대로 평탄지의 형성과정에 관한 일반모델을 제시하였다. 한반도에서는 산지와 뚜렷하게 대비되는 평탄지가 존재하고 있으며, 그 형태적 특성과 위치를 종합적으로 고려할 경우, 용암대지와 하해혼성평탄지, 구릉성평탄지, 산간분지, 고위평탄지, 하천주변평탄지 등으로 분류가 가능하다. 평탄지의 특성은 융기 및 해수면 변화와 관련된 기준면의 변동과 지질단위들의 위상관계에 의해 결정되며, 이들 평탄지들이 독립적으로 존재하는 것은 아니가 하천을 따른 삭박환경의 변화에 따라 동적으로 연계되어 있다. 이 연구에서 제시하는 평탄지 형성의 일반모델은 과거 개별적으로 이루어져 왔던 한반도의 평탄면과 각종 침식지형, 그리고 단구지형에 대한 연구들이 평탄지의 분포와 특성을 통해 통합될 수 있는 가능성을 제시해 준다.

• 생명과학회지
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• 제16권7호
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• pp.1195-1198
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• 2006

The importance of wild plant resources along with the development of high biotecyhnology is highlighted for exploitation of new materials which can make the added value. The goal of this study is providing fundamental data bases for developing new materials through the analyses of morphological characteristics, antibiotics and molecular cytogenetics in Baegseon (Dictamnus dasycarpus Turcz.). Baegseon has several characteristics; there are two types of flower color, pink and white, the seed germination starts about February 20th, the maximum flowering season is around May 17th in southern Korea, the growth period is about 60 days. The number of chromosomes are 2n=2x=36, the size of chromosomes in metaphase is $4.2{\sim}8.1{\mu}m$. The amount of 2C nuclear DNA is 1.93pg, and there is no variation of genome size amoung varieties. The extraction juice of baegseon young roots has the excellent antibiotic activity against the mold (Botrytis cinerea).

• 한국정보통신학회논문지
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• 제19권9호
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• pp.2129-2137
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• 2015

국내외적으로 무인자동차에 대한 연구와 개발이 활발히 진행되고 있다. 무인자동차를 성공적으로 구현하기 위해서는 매우 많은 요소 기술들을 필요로 한다. 특히 교통신호등의 검출과 인식 시스템은 무인자동차에서 컴퓨터 비전 기술의 핵심적인 요소기술로 주목 받고 있다. 최근까지 제안된 대부분의 교통 신호등 인식 방식들은 잡음과 환경적인 요소에 따라 의존적인 색깔 성분 분석 방법을 사용함으로써 인식률 개선에 있어 제한적인 성능 특성을 갖고 있다. 본 논문에서는 이러한 기존의 방식의 한계를 극복하기 위해 교통신호등이 갖는 형태학적인 특성을 최대한 고려한 방법을 제안한다. 제안한 방식은 색깔 성분과 사각형 특성, 원형 특성과 같은 형태학적 특성을 동시에 고려함으로써 인식 효율을 크게 증대시킨다. 다양한 모의실험을 통하여 제안한 방식은 교통신호등 인식률뿐만 아니라 오인식률 성능을 크게 개선시킬 수 있음을 보인다.

• 한국발생생물학회지:발생과생식
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• 제23권1호
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• pp.11-19
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• 2019

The study was conducted to investigate the effects of alpha-linolenic acid (ALA) combined with bovine serum albumin (BSA) or methyl-beta-cyclodextrin (MBCD) on plasma and acrosomal membrane damages, mitochondrial activity, morphological abnormality, motility, and oxidative stress in frozen-thawed boar sperm. In previous our study, 3 ng/mL ALA had been shown protective effect during freezing process of boar sperm. Therefore, we used 3 ng/mL ALA in present study and ALA was combined with same molar ratio of BSA or MBCD (ALA+BSA and ALA+MBCD, respectively). To confirm the effect of two carrier proteins, same volume of BSA and MBCD without ALA were added during cryopreservation. Membrane damage, mitochondrial activity, reactive oxygen species (ROS) and lipid peroxidation (LPO) levels were measured using flow cytometry, and movement of sperm tail as motility parameter and morphological abnormality were observed under light microscope. In results, all of sperm parameters were enhanced by ALA combined with BSA or MBCD compared to control groups (p<0.05). Mitochondrial activity, morphological abnormality, ROS and LPO levels in ALA+BSA or MBCD groups were no significant difference compared with ALA, BSA and MBCD treatment groups. On the other hand, plasma and acrosomal membrane intact, and sperm motility in ALA+MBCD group were higher than single treatment groups (p<0.05), whereas ALA+BSA did not differ. Our findings indicate that carrier proteins such as BSA and MBCD could improve the effect of ALA during cryopreservation of boar sperm, and treatment of ALA with carrier proteins enhance membrane integrity, mitochondrial activity through reduction of ROS-induced LPO.