• Title/Summary/Keyword: Morphological characterization

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Molecular and Morphological Characterization of a Taxol-Producing Endophytic Fungus, Gliocladium sp., from Taxus baccata

  • Sreekanth, D.;Sushim, G.K.;Syed, A.;Khan, B.M.;Ahmad, A.
    • Mycobiology
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    • v.39 no.3
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    • pp.151-157
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    • 2011
  • The endophytic fungal populations of different tissues of Taxus baccata grown at high altitudes in West Bengal, India were explored. These isolated fungal populations represented different genera, which were screened for taxol production using immunoassay technique. The culture AAT-TS-$4_1$ that produced taxol was identified as Gliocladium sp. based on its cultural, morphological characteristics, internal transcribed spacer, and 18S rRNA sequence analysis. Kinetics of taxol production as a function of culture growth were investigated.

Characterization of a Brown Rot Fungus Isolated from Dwarf Flowering Almond in Korea

  • Shim, Myoung-Yong;Jeon, Young-Jae;Kim, Seong-Hwan
    • Mycobiology
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    • v.35 no.1
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    • pp.30-35
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    • 2007
  • The fruits showing brown rot symptom on dwarf flowering almond were found in Gongju, Chungchungnam-Do in Korea in July 2005. Small water-soaked lesions on the fruits were initiated, and gradually developed to soft rot covered with gray conidia. Then the diseased fruits were shrunk and became grayish-black mummies. A fungus was isolated from the diseased fruit and its morphological, cultural and molecular genetic characteristics were investigated. Typical blastospores of Monilinia spp. were observed under a light microscope both from tissues of the diseased fruits and from PDA-grown cultures. The fungus grew well at $25^{\circ}C$ and on PDA. The ITS ribosomal DNA region (650 bp) of the fungus was amplified by PCR and analyzed. Comparative data on ITS sequence homology among Monilinia spp., ITS sequence-based phylogram and morphological characteristics showed that the fungus is Monilinia fructicola. This is the first report on Monilinia fructicola causing brown rot on fruits of dwarf flowering almond in Korea.

Characterization of Paecilomyces variotii and Talaromyces amestolkiae in Korea Based on the Morphological Characteristics and Multigene Phylogenetic Analyses

  • Nguyen, Thi Thuong Thuong;Paul, Narayan Chandra;Lee, Hyang Burm
    • Mycobiology
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    • v.44 no.4
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    • pp.248-259
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    • 2016
  • During fungal diversity surveys of the order Eurotiales in Korea, two fungal strains, EML-DG33-1 and EML-NCP50, were isolated from samples of rat dung and fig tree leaf collected at a garden located in Gwangju in 2014. To complete the National Species List of Korea, it is a prerequisite to verify whether many questionable species, which were previously recorded but not confirmed, indeed present in Korea. Herein, the isolates were confirmed as undescribed species, Paecilomyces variotii and Talaromyces amestolkiae based on the combination of morphological and phylogenetic analyses of multigenes including the rDNA internal transcribed spacer, ${\beta}-tubulin$, and RNA polymerase II subunit 2.

Morphology and Molecular Phylogeny of Pseudocercospora fatouae Associated with Hypophyllous Leaf Mold on Fatoua villosa in Korea

  • Kui-Jae Lee;In-Young Choi;Lamiya Abasova;Joon-Ho Choi;Jung-Hee Park;Hyeon-Dong Shin
    • The Korean Journal of Mycology
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    • v.51 no.2
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    • pp.147-153
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    • 2023
  • Dark brown leaf molds growing in a vein-limited pattern have been continuously observed on the lower leaf surface of Fatoua villosa in Korea. Fifteen samples deposited in the Korea University herbarium were examined for fungal morphological characterization. Two monoconidial isolates were obtained in 2022, and molecular analysis was performed based on a combined multigene dataset of the internal transcribed spacer (ITS) region and partial actin (actA), translation elongation factor 1-alpha (tef1), and DNA-directed RNA polymerase II second-largest subunit (rpb2). Based on morphological features and molecular phylogenetic analysis, the fungus Pseudocercospora fatouae was identified. To the best of our knowledge, it is the first study reporting the presence of P. fatouae on F. villosa in Korea.

Morphological and Molecular Characterization of Podosphaera Powdery Mildew on Fatoua villosa in Korea

  • In-Young Choi;Lamiya Abasova;Joon-Ho Choi;Chong-Kyu Lee;Hyeon-Dong Shin
    • The Korean Journal of Mycology
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    • v.50 no.3
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    • pp.243-248
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    • 2022
  • In Japan and China, Podosphaera pseudofusca causes powdery mildew in Fatoua villosa. During a taxonomic revision of Korean powdery mildew fungi, isolates newly collected from F. villosa were studied. Through morphological examination and molecular phylogenetic analyses of the internal transcribed spacer region and large subunit gene of rDNA, the powdery mildew fungus was identified as Podosphaera xanthii. In this study, we propose the merging of P. pseudofusca sensu Braun into the P. xanthii complex. To the best of our knowledge, this is the first report of Podosphaera powdery mildew on F. villosa in Korea.

Characterization of Three Species of Endophytic Fungi Isolated from Conifer Leaves in Korea

  • Jae-Wook Choi;Ju-Hui Gwon;Jong-Chul Lee;Hyeok Park;Ahn-Heum Eom
    • The Korean Journal of Mycology
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    • v.50 no.3
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    • pp.173-181
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    • 2022
  • Endophytic fungi were isolated from needle leaves of two conifer species in Korea. The fungal isolates were identified based on their morphological characteristics and phylogenetic analyses of their internal transcribed spacer and large-subunit rDNA regions. Three species of endophytic fungi, namely Celosporium laricicola, Neocatenulostroma germanicum, and Phaeophleospora eucalypticola were the first records in Korea. In this study, we reported the morphological and phylogenetic characteristics of these fungi.

Conservation of Multivoltine Silkworm (Bombyx mori L.) Germplasm in India - An Overview

  • Kumaresan, P.;Sinha, R.K.;Mohan, B.;Thangavelu, K.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.9 no.1
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    • pp.1-13
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    • 2004
  • Indian multivoltine mulberry silkworm (Bombyx mori L.) strains are poor in silk productivity and fibre quality. However, they are commercially exploited for the past five decades either in the form of pure breeds or cross breeds because of their adaptability to adverse climatic condition and acclimatization to specific regions and seasons. In the present paper conservation strategies of multivoltine silkworm genetic resources are dealt along with detailed information on accessibility of genetic resources, method of genetic resources conservation, characterization of genetic resources for morphological and productivity traits of economic importance. Selection of best ten accessions based on various economic parameters including yarn quality and their scope for future utilization are discussed.

Characterization of both adhesion and interfacial interaction between optical fiber coating and structural

  • Brotzu, A.;Felli, F.;Fiori, L.;Caponero, M.A.
    • Smart Structures and Systems
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    • v.4 no.4
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    • pp.439-448
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    • 2008
  • Optical fiber sensors are by now broadly accepted as an innovative and reliable device for structural health monitoring, to be used either embedded into or bonded on structures. The accuracy of the strain measurement achievable by optical fiber sensors is critically dependent on the characteristics of the bonding of the various interface layers involved in the sensor bonding/embedding (structure material and gluing agent, fiber coating and gluing agent, fiber coating and fiber core). In fact, the signal of the bonded/embedded optical fiber sensor must correspond to the strain experienced by the monitored structure, but the quality of each involved interface can affect the strain transfer. This paper faces the characterization, carried on by both mechanical tests and morphological analysis, of the strain transfer function resulting with epoxidic and vinylester gluing agent on polyimide and acrylate coated optical fibers.

Studies on Borassus fruit fiber and its composites with Polypropylene

  • Sudhakara, P.;Obi Reddy, K.;Prasad, C. Venkata;Jagadeesh, Dani.;Kim, H.S.;Kim, B.S.;Bae, S.I.;Song, J.I.
    • Composites Research
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    • v.26 no.1
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    • pp.48-53
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    • 2013
  • This paper summarizes the structural characterization of borassus fruit fibers by means of various characterization techniques, optimization of alkali treatment of borassus fruit fine fibers (BFF) with a 5% concentration sodium hydroxide solution for different time intervals (1, 4, 8 and 12 h) and the changes occurring in borassus fibers. This paper also discusses the manufacturing of BFF/PP compotes using MAPP as a compatibilizer in addition to alkali treatment. Composites were evaluated for their mechanical and morphological properties. The tensile strength and modulus, flexural strength and modulus and impact strength were increased for alkali treated/MAPP composites by 4.5%, 17%, 17.2 %, 9% and 10% respectively.

Characterization of haemocytes in the surf clam Mactra veneriformis

  • Yu, Jin-Ha;Park, Kyung-Il;Park, Sung-Woo
    • Journal of fish pathology
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    • v.22 no.3
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    • pp.305-316
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    • 2009
  • Haemocyte characterization in the surf clam, Mectra veneriformis was carried out based on morphological, cytochemical, and phagocytic characteristics. The haemocytes were classified into two cell types, granulocytes and agranulocytes on the basis of presence of cytoplasmic granules. Granulocytes were then classified again into 2 types, large eosinophilic granulocytes and small eosinophilic granulocytes after staining with May-Grünwald Giemsa. In electron microscopy, both types of granulocytes contained electron-dense and electron-lucent cytoplasmic granules. Agranulocytes (hyalinocytes) were also divided into two cell types, large agranulocytes and small agranulocytes based on their sizes. Both cell types did not have granules in cytoplasm. Granulocyte and agranulocyte were negative for the enzyme activities of alkaline phosphatase, peroxidase, and $\beta$-glucuronidase but positive for phenoloxidase and acid phosphatase activities. Both types of haemocytes have phagocytic activity, with the exception of small agranulocyte, and granulocytes seemed more active in this respect than agranulocytes. This present study is the first study to characterize haemocytes of M. veneriformis.