• 제목/요약/키워드: Morphological and cultural characteristics

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Anthracnose of Gardenia (Gardenia jasminoides for, grandiflora Makino) Caused by Glomerella cingulata

  • Kim, Gyoung-Hee;Lee, Jae-Goon;Hur, Jae-Seoun;Koh, Young-Jin
    • The Plant Pathology Journal
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    • 제18권4호
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    • pp.228-230
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    • 2002
  • Anthracnose symptoms caused by Glomerella cingulata were observed on leaves and stems of gardenia in Sunchon, Jeonnam in Korea in 2000. Symptoms on infected plants typically appeared as irregularly circular, dark-brown ring spots and water-soaked brown lesions. Based on cultural and morphological characteristics, the fungus (G-00-03 isolate) from the diseased plants was identified as Colletotrichum gloeosporioides, and its teleomorph stage was Glomerella cingulata. Healthy gardenia artificially inoculated with fungal spores showed anthracnose symptom 7 days after inoculation. This is the first report of gardenia anthracnose caused by Glomerella cingulata in Korea.

Anthracnose of Rapsberry(Rubus coreanus) Caused by Colletotrichum coccodes in Korea

  • Kim, J. H.;S. S. Cheong;J, Ryu;Park, J. S.;Park, Y. G.;Lee, W. H.
    • 한국식물병리학회:학술대회논문집
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    • 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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    • pp.132.2-132
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    • 2003
  • Anthracnose occurred on rapsberry grown in Gochang areas of Korea in 2003. The disease incidence was ranged from 1.1 to 2.6%. Anthracnose of rapsberry appeared as dark brown circular spots on naturally infected stems. The symptoms of infected stems were small brown to dark brown spots and gradually enlarged larger cylindrical dark brown lesions. The causal fungus of anthracnose isolated from the diseased plants was identifed as Colletotrichum coccodes based on the morphological and cultural characteristics. All isolates of C. coccodes were produced similar symptoms on the host leaves by artificial inocultion.

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Occurrence of Tulip Fire Caused by Botrytis tulipae in Korea

  • Hong, Sung-Kee;Kim, Wan-Gyu;Cho, Weon-Dae;Kim, Hong-Gi
    • The Plant Pathology Journal
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    • 제18권2호
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    • pp.106-108
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    • 2002
  • Severe spotted lesions were observed on tulip plants grown in Asan, Yongin, and Icheon areas in Korea during a disease survey in 2000 and 2001. Diseased plants with severe symptoms were blighted and rotted at the late stage of disease development. A total of 15 isolates was obtained from the infected plant parts of tulip. All the isolates were identified as Botrytis tulipae based on their morphological and cultural characteristics. Three isolates of B. tulipae were tested for their pathogenicity to tulip by artificial inoculation. Spotted lesions similar to those observed in the fields were induced on tulip leaves. This is the first report of tulip fore disease caused by B. tulipae in Korea.

해양에서 분리된 Klebsiella sp. KCL-1에 의한 원유분해 특성의 규명 (Characterization of Crude Oil Degradation by Klebsiella sp. KCL-1 Isolated from Sea Water)

  • 차재영;정선용;조용수;최용락;김범규;이영춘
    • 한국미생물·생명공학회지
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    • 제27권6호
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    • pp.452-457
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    • 1999
  • Several bacterial strains utilizing crude oil as their sole carbon and energy sources were isolated from marine. One of the strains named KCL-1 showed the highest degradative activity for crude oil and the best growth rate. This strain was identified as a Klebsiella sp. based on the morphological, biochemical, and physiological characteristics. The optimum cultural conditions were as follows; $32^{\circ}C$~$37^{\circ}C$ for temperature and 7.0 for initial pH. Additionally, the optimal concentration of sodium chloride was 3.0%, indicating that this strain was derived from seawater. KCL-1 could use several kinds of n-alkane hydrocarbons from octadecane to hexacosane as a sole carbon source. The degradation of crude oil by KCL-1 was stimulated by addition of octadecane in the culture. The emulsifying activity by KCL-1 was highest after 3 days of cultivation under the condition of 3.0% sodium chloride, pH 7.0 and $37^{\circ}C$.

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광합성세균에 의한 수소 생산에 관한 연구 (제 1보) 광합성균의 분리 및 동정 (Studies on Hydrogen Evolution by Photosynthetic Bacteria(Part 1) Isolation and Identification of the Photosynthetic Bacteria)

  • 배무;양성우;고영희
    • 한국미생물·생명공학회지
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    • 제10권1호
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    • pp.27-32
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    • 1982
  • 광합성세균에 의한 수소산을 목적으로 성남근교의 논 토양으로부터 수소를 다량 생산하는 비유황세균 1주를 분리하고 동정하였다. 이 균주의 생리적 형태적 및 배양상의 특성을 규명한 결과 Rhodopseudomonas gelatinosa K-13으로 동정되었다.

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김치에서 분리한 Lactobacillus sp. JC-7과 Lactobacillus acidophilus 88간의 Electrofusion 최적조건 설정

  • 조영배;최현정;백형석;전홍기
    • 한국미생물·생명공학회지
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    • 제25권2호
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    • pp.121-128
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    • 1997
  • A lactic acid bacterium was isolated from kimchi. The isolated strain was identified as the genus Lactobacillus through its morphological, cultural, and physiological characteristics and named as Lactobacillus sp. JC-7. The optimum conditions for the electrofusion between streptomycin (2.5 mg/ml) resistant mutant of Lactobacillus acidophilus 88 and kanamycin (600 $\mu$g/ml) resistant mutant of Lactobacillus sp. JC-7 were evaluated. The highest number of fusants were obtained at a capacitance value of 120 msec (1670 $\mu$F), a field strength of 100 V/cm, and a pulse controller setting of 72$\Omega$. The optimum pH of electroporation buffer was 7.5 and the concentration of divalent cation was 1 mM Mg$^{2+}. Electrofusants were efficiently obtained by addition 20% polyethylene glycol to elec- troporation buffer. The yield of fusion was better than that of using polyethylene glycol mediated chemical induction.

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Production of Antihypertensive Angiotensin I-Converting Enzyme Inhibitor from Malassezia pachydermatis G-14

  • Jeong, Seung-Chan;Kim, Jae-Ho;Kim, Na-Mi;Lee, Jong-Soo
    • Mycobiology
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    • 제33권3호
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    • pp.142-146
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    • 2005
  • To produce a novel antihypertensive angiotensin I-converting enzyme (ACE) inhibitor from yeast, a yeast isolate, designated G-14 showing the highest ACE inhibitory activity was obtained and identified as Malassezia pachydermatis based on morphological, biochemical and cultural characteristics. The maximal extracellular ACE inhibitor production was obtained from M. pachydermatis G-14 when the strain was cultured in YEPD medium containing 0.5% yeast extract, 3.0% peptone and 2.0% glucose at $30^{\circ}C$ for 24 h and the final ACE inhibitory activity was 48.9% under the above condition.

Identification of Aspergillus Strain with Antifungal Activity Against Phytophthora Species

  • KANG SUNG WOO;HONG SUK IN;KIM SEUNG WOOK
    • Journal of Microbiology and Biotechnology
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    • 제15권2호
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    • pp.227-233
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    • 2005
  • Fungal strain CGF was isolated from the soil of ChungNam Province, South Korea. Based on the 28S rDNA sequence analysis and the sequence of the internal transcribed spacer (ITS) region of ribosomal DNA, together with morphological and cultural characteristics, this strain was identified as Aspergillus sclerotiorum and renamed Aspergillus sclerotiorum CGF. This is the first strain of Aspergillus sclerotiorum identified in Korea. When the antifungal activity of A. sclerotiorum CGF was evaluated, among the phytopathogenic fungi, mycelial growth of only Phytophthora species was inhibited. Oermination of P. capsid zoospore was also inhibited. The bioactive compound of A. sclerotiorum CGF was highly thermo- and pH-stable.

알칼리성 Protease를 생성하는 알칼리성 세균 및 그 효소활성에 관한 연구 (Studies on the Alkalophilic Bacteria Producing Alkaline Protease and its Enzyme Activities)

  • 박수진;이강만;배무
    • 약학회지
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    • 제34권1호
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    • pp.47-53
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    • 1990
  • Alkalophilic bacteria isolated from compost were selected, identified and tested for production of alkaline protease. The bacterium was tentatively assigned to Bacillus sp. based on the morphological, cultural and biochemical characteristics. The optimum pH of growth was 10 Galactose and Sodium glutamate enhanced the production of alkaline protease. The protease was most active at pH 11.0 and $60^{\circ}C$ and stable in the range of pH 5-11 and temp. $30^{\circ}-55^{\circ}C$. The protease was stabilized by the presence of calcium salts.

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Grovesinia moricola occurring on Parthenocissus tricuspidata

  • Shin, Hyeon-Dong;Choi, Young-Joon;Hong, Sun-Hee;Lee, Yong-Ho
    • 한국균학회지
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    • 제47권3호
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    • pp.271-274
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    • 2019
  • Between August and September 2017, zonate leaf spots with severe defoliation were observed on Parthenocissus tricuspidata in Gimcheon, Korea. The initial symptoms included water-soaked, gray to grayish brown, circular or irregular-shaped leaf spots. As the disease progressed, the spots enlarged, coalesced, and became cream-colored with characteristic target-shaped rings, leading to leaf blight and premature defoliation. Based on cultural and morphological characteristics, the fungus associated with the symptoms was identified as Grovesinia moricola. The identity of this fungus was confirmed by analyzing sequences of the internal transcribed spacer region of ribosomal DNA. The pathogenicity of the isolate was demonstrated by artificial inoculation. To our knowledge, this is the first report of G. moricola occurring on P. tricuspidata globally as well as in Korea.