• Title/Summary/Keyword: Morphological Study

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Detailed morphological analysis of axolotl sperm

  • Keskin, Ilknur;Gurgen, Duygu Gursoy;Avinca, Didem;Ozdemir, Ekrem Musa;Keskin, Suat Utku;Karabulut, Seda
    • Korean Journal of Veterinary Research
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    • v.61 no.3
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    • pp.25.1-25.7
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    • 2021
  • The axolotl has extraordinary regeneration capacity compared to other vertebrates. This remarkable potential has been attributed to its life-long neoteny, characterized by the exhibition of embryonic characteristics at the adult stage. A recent study provided a detailed morphological analysis of the sperm morphology of the Ambystoma mexicanum using routine and detailed histological techniques. The primary purpose of the present study is to describe a simple and inexpensive method for evaluating the morphology of axolotl sperm. In this study, spermatophore structures were collected and spread on slides and air-dried. The slides were stained with periodic acid Schiff, toluidine blue, Masson's trichrome, Giemsa, Spermac, and Diff-Quik dye for a morphological examination. The slides were coated with gold/palladium for a scanning electron microscopy examination. The sperm of the axolotl consisted of an elongated head, a neck, and a flagellum covered with an undulating membrane. The lengths of the midpiece, tail, and head were 8.575 ㎛, 356.544 ㎛, and 103.661 ㎛, respectively. In the flagellum part, the wavy membrane structure, whose function has not been explained, surrounds the tail. The data obtained from this study will constitute an important step in designing future research on the reproductive and regeneration capacity of the axolotl.

Comparative Analysis of DTM Generation Method for Stream Area Using UAV-Based LiDAR and SfM (여름철 UAV 기반 LiDAR, SfM을 이용한 하천 DTM 생성 기법 비교 분석)

  • Gou, Jaejun;Lee, Hyeokjin;Park, Jinseok;Jang, Seongju;Lee, Jonghyuk;Kim, Dongwoo;Song, Inhong
    • Journal of The Korean Society of Agricultural Engineers
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    • v.66 no.3
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    • pp.1-14
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    • 2024
  • Gaining an accurate 3D stream geometry has become feasible with Unmanned Aerial Vehicle (UAV), which is crucial for better understanding stream hydrodynamic processes. The objective of this study was to investigate series of filters to remove stream vegetation and propose the best method for generating Digital Terrain Models (DTMs) using UAV-based point clouds. A stream reach approximately 500 m of the Bokha stream in Icheon city was selected as the study area. Point clouds were obtained in August 1st, 2023, using Phantom 4 multispectral and Zenmuse L1 for Structure from Motion (SfM) and Light Detection And Ranging (LiDAR) respectively. Three vegetation filters, two morphological filters, and six composite filters which combined vegetation and morphological filters were applied in this study. The Mean Absolute Error (MAE) and Root Mean Square Error (RMSE) were used to assess each filters comparing with the two cross-sections measured by leveling survey. The vegetation filters performed better in SfM, especially for short vegetation areas, while the morphological filters demonstrated superior performance on LiDAR, particularly for taller vegetation areas. Overall, the composite filters combining advantages of two types of filters performed better than single filter application. The best method was the combination of Progressive TIN (PTIN) and Color Indicies of Vegetation Extraction (CIVE) for SfM, showing the smallest MAE of 0.169 m. The proposed method in this study can be utilized for constructing DTMs of stream and thus contribute to improving the accuracy of stream hydrodynamic simulations.

Morphological Analysis of Wear Particles using Fractal Parameters

  • Cho, Y.S.;Park, H.S.
    • Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
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    • 2002.10b
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    • pp.457-458
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    • 2002
  • The fractal dimension is the characteristics that can quantitatively define the irregularity in natural. It is useful in describing the morphology or various rubbed surface for hydraulic piston motor instead of the stylus profiling method. But fractal parameters had not constructed on the morphological characteristic or rubbed surface because of the insufficient knowledge about a conception of fractal dimension. In this study, for the purpose or applying fractal I parameters practically, we have suggested way to establish the morphological characteristic of rubbed surface with fractal parameters, and we carried out an experiment on the lubricant friction and wear by using Ball-ON-Disk type tester. Materials were the brass and the bronze which are used to slipper-pad in the hydraulic piston motor. We searched for fractal parameters or surface structure with the digital image processing, Surface fractal dimension can be determined by sum of intensity difference of surface pixel. Using the image processing and fractal parameters for rubbed surface in the friction and wear test, morphology of rubbed sur race can be effectively obtained by fractal dimensions.

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Alveolar Fricative Sound Errors by the Type of Morpheme in the Spontaneous Speech of 3- and 4-Year-Old Children (자발화에 나타난 형태소 유형에 따른 3-4세 아동의 치경마찰음 오류)

  • Kim, Soo-Jin;Kim, Jung-Mee;Yoon, Mi-Sun;Chang, Moon-Soo;Cha, Jae-Eun
    • Phonetics and Speech Sciences
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    • v.4 no.3
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    • pp.129-136
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    • 2012
  • Korean alveolar fricatives are late-developing speech sounds. Most previous research on phonemes used individual words or pseudo words to produce sounds, but word-level phonological analysis does not always reflect a child's practical articulation ability. Also, there has been limited research on articulation development looking at speech production by grammatical morphemes despite its importance in Korean language. Therefore, this research examines the articulation development and phonological patterns of the /s/ phoneme in terms of morphological types produced in children's spontaneous conversational speech. The subjects were twenty-two typically developing 3- and 4-year-old Koreans. All children showed normal levels in three screening tests: hearing, vocabulary, and articulation. Spontaneous conversational samples were recorded at the children's homes. The results are as follows. The error rates decreased with increasing age in all morphological contexts. Also, error percentages within an age group were significantly lower in lexical morphemes than in grammatical morphemes. The stopping of fricative sounds was the main error pattern in all morphological contexts and reduced as age increased. This research shows that articulation performance can differ significantly by morphological contexts. The present study provides data that can be used to identify the difficult context for articulatory evaluation and therapy of alveolar fricative sounds.

Comparison of bivalves of Family Pinnidae from Southern Vietnam: A morphometric approach

  • Silina, Alla V.
    • The Korean Journal of Malacology
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    • v.27 no.4
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    • pp.345-351
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    • 2011
  • Comparison of different morphological characteristics of bivalves is very useful for distinguishing species. Therefore, this study used a morphometric approach to document patterns of phenotypic change through the specimens of family Pinnidae inhabiting the bottom sediments at the coasts of An Thoi Archipelago (south-eastern Gulf of Thailand), in that way excluding intraspecific variations among different populations along a wide geographical range. It was revealed that individuals determined as Pinna trigonium separated from specimens of Pinna nigra and Atrina vexillum at high level, but P. nigra and A. vexillum were the single species A. vexillum, as it is assumed now. Also, it was found that both groups of P. trigonium individuals and A. vexillum specimens divided into tree subgroups unified the specimens with the similar morphometric parameters: small, medium and large within the populations, though there were no evident divisions into subgroups in the size distributions of the populations. In addition to the interspecific morphological variations, the species also showed significant intraspecific morphological variations even in the same population. Most probable reason for the appearance of the intraspecific morphological variations and division of all specimens into size-specific subgroups in the population is the fact that the growth of Pinnidae bivalves is not isometric.

Morphological Features of Pollen Grains in Portulaca

  • Kim, InSun
    • Applied Microscopy
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    • v.43 no.2
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    • pp.75-80
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    • 2013
  • The morphological features of pollen grains collected from the broad-leaved and cylindrical-leaved Portulaca species of the Hawaiian Islands were investigated. The variation in size and surface pattern were examined using scanning electron microscopy and statistical analysis. Pollens of the Portulaca were apolar grains of monads exhibiting apertures, colpi, and relatively thin echini. Of particular interest was the differing size of pollen grains among the species studied. Pollens of the broad-leaved Portulaca were considerable in size with a length of about $73{\sim}86{\mu}m$, while the cylindrical-leaved Portulaca tended to have smaller pollen grains ranging from 50~65 in diameter. The smallest pollens were found in cultivar 2 having an average of $43{\mu}m$. The pollen was intectate, and the exine surface appeared granulous, having sculptured elements of spinules and puncta. Two types of puncta were discerned; one forming an operculum, and the other, a simple perforation. Numerous spinules and small puncta were found throughout the cylindrical-leaved Portulaca. In the present study, morphological features of pollen grains from ten Hawaiian Portulaca species, including endemic and unknown taxa, revealed the aforementioned pattern of variation. This paper aim to provide morphological information that could be of phylogenetic value within the Hawaiian Portulaca.

The Syllable Frequency Effect in Semantic Categorization Tasks in Korean

  • Kim, Ji-Hye;Kwon, You-An;Nam, Ki-Chun
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.5 no.10
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    • pp.1879-1890
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    • 2011
  • Previous studies of syllable frequency effects have proposed that inhibitory effects due to high first syllable frequency were the products of competitions between activated lexical candidates within a lexical level. However, these studies have primarily used lexical decision tasks to examine the nature of syllable frequency effects. This study investigates whether a syllable frequency effect can arise in semantic categorization tasks and whether phonologically or orthographically defined syllables interact with semantically related variables such as morphological family size. If the syllable frequency effect was created by activations and competitions on a lexical level, it is highly possible that the effect was related to semantic categorization tasks. To test this hypothesis, we conducted two experiments. In Experiment 1, morphological family size and phonological syllable frequency were factorially manipulated. In Experiment 2, morphological family size and orthographic syllable frequency were factorially manipulated. The results demonstrate that morphemes have no relationship with phonological syllables but do with orthographic syllables. This suggests that phonological syllables and orthographic syllables have different roles in the syllable frequency effect on visual word recognition process.

Probabilistic Segmentation and Tagging of Unknown Words (확률 기반 미등록 단어 분리 및 태깅)

  • Kim, Bogyum;Lee, Jae Sung
    • Journal of KIISE
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    • v.43 no.4
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    • pp.430-436
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    • 2016
  • Processing of unknown words such as proper nouns and newly coined words is important for a morphological analyzer to process documents in various domains. In this study, a segmentation and tagging method for unknown Korean words is proposed for the 3-step probabilistic morphological analysis. For guessing unknown word, it uses rich suffixes that are attached to open class words, such as general nouns and proper nouns. We propose a method to learn the suffix patterns from a morpheme tagged corpus, and calculate their probabilities for unknown open word segmentation and tagging in the probabilistic morphological analysis model. Results of the experiment showed that the performance of unknown word processing is greatly improved in the documents containing many unregistered words.

Regional Heterogeneity of Morphological Changes in Cultured Rat Astrocytes

  • Won, Chung-Kil;Oh, Young-S.
    • The Korean Journal of Physiology and Pharmacology
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    • v.5 no.6
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    • pp.467-477
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    • 2001
  • We examined astrocyte regional heterogeneity in their morphological changes in response to various stimuli. Astrocytes were cultured from six different neonatal rat brain regions including cerebral cortex, hippocampus, cerebellum, mid brain, brain stem and hypothalamus. Astrocyte stellation was induced by serum deprivation and the maximum stellation in different regional astrocytes was achieved after 2 h. After 24 h, in all astrocyte cultures, the level of stellation returned to their original level. Cerebellar or hypothalamic astrocytes were the most or the least sensitive, respectively, to serum deprivation. The order of maximum sensitivity to serum deprivation among different regional astrocytes was: cerebellum>mid $brain{\ge}hippocampus,\;brain\;stem{\ge}cerebral$ cortex>hypothalamus. Isoproterenol-induced astrocyte stellation was also examined in different regional astrocytes, and similar order of maximum sensitivity as in serum deprivation was observed. Next a possible developmental effect on astrocyte morphological changes was examined in cerebral cortex and cerebellum astrocytes cultured from postnatal day 1 (P1), P4 and P7 rat brains. A much higher sensitivity of cerebellum astrocytes to serum deprivation as well as isoproterenol treatment was consistently observed in P1, P4 and P7-derived astrocytes compared to cerebral cortex astrocytes. The present study demonstrates different regional astrocytes maintain different levels of morphological plasticity in vitro.

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Silymarin Inhibits Morphological Changes in LPS-Stimulated Macrophages by Blocking NF-${\kappa}B$ Pathway

  • Kim, Eun Jeong;Lee, Min Young;Jeon, Young Jin
    • The Korean Journal of Physiology and Pharmacology
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    • v.19 no.3
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    • pp.211-218
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    • 2015
  • The present study showed that silymarin, a polyphenolic flavonoid isolated from milk thistle (Silybum marianum), inhibited lipopolysaccharide (LPS)-induced morphological changes in the mouse RAW264.7 macrophage cell line. We also showed that silymarin inhibited the nuclear translocation and transactivation activities of nuclear factor-kappa B (NF-${\kappa}B$), which is important for macrophage activation-associated changes in cell morphology and gene expression of inflammatory cytokines. BAY-11-7085, an NF-${\kappa}B$ inhibitor, abrogated LPS-induced morphological changes and NO production, similar to silymarin. Treatment of RAW264.7 cells with silymarin also inhibited LPS-stimulated activation of mitogen-activated protein kinases (MAPKs). Collectively, these experiments demonstrated that silymarin inhibited LPS-induced morphological changes in the RAW264.7 mouse macrophage cell line. Our findings indicated that the most likely mechanism underlying this biological effect involved inhibition of the MAPK pathway and NF-${\kappa}B$ activity. Inhibition of these activities by silymarin is a potentially useful strategy for the treatment of inflammation because of the critical roles played by MAPK and NF-${\kappa}B$ in mediating inflammatory responses in macrophages.