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Effects of a specific blend of essential oils on apparent nutrient digestion, rumen fermentation and rumen microbial populations in sheep fed a 50:50 alfalfa hay:concentrate diet

  • Khateri, N.;Azizi, O.;Jahani-Azizabadi, H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.3
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    • pp.370-378
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    • 2017
  • Objective: An experiment was conducted to investigate the effects of a specific mixture of essential oils (MEO), containing thyme, clove and cinnamon EO, on rumen microbial fermentation, nutrient apparent digestibility and blood metabolites in fistulated sheep. Methods: Six sheep fitted with ruminal fistulas were used in a repeated measurement design with two 24-d periods to investigate the effect of adding MEO at 0 (control), 0.8, and 1.6 mL/d on apparent nutrient digestibility, rumen fermentation characteristics, rumen microbial population and blood chemical metabolites. Animals were fed with a 50:50 alfalfa hay:concentrate diet. Results: Ruminal pH, total volatile fatty acids (VFA) concentration, molar proportion of individual VFA, acetate: propionate ratio and methane production were not affected with MEO. Relative to the control, Small peptides plus amino acid nitrogen and large peptides nitrogen concentration in rumen fluid were not affected with MEO supplementation; while, rumen fluid ammonia nitrogen concentration at 0 and 6 h after morning feeding in sheep fed with 1.6 mL/d of MEO was lower (p<0.05) compared to the control and 0.8 mL/d of MEO. At 0 h after morning feeding, ammonia nitrogen concentration was higher (p<0.05) in sheep fed 0.8 mL/d of MEO relative to 1.6 mL/d and control diet. Ruminal protozoa and hyper ammonia producing (HAP) bacteria counts were not affected by addition of MEO in the diet. Relative to the control, no changes were observed in the red and white blood cells, hemoglobin, hematocrit, glucose, beta-hydroxybutyric acid, cholesterol, total protein, albumin, blood urea nitrogen and aspartate aminotransferase and alanine aminotransferase concentration. Apparent total tract digestibility of dry matter, crude proten, organic matter, and neutral detergent fiber were not influenced by MEO supplementation. Conclusion:The results of the present study suggested that supplementation of MEO may have limited effects on apparent nutrient digestibility, ruminal fermentation and protozoa and HAP bacteria count, blood cells and metabolites.

Establishment for Improving Productivity of Cattle by Fecal Steroid and Milk Urea Nitrogen Analysis - I. Development of Enzyme-linked Immunosorbent Assay for Progesterone and Milk Urea Nitrogen Analysis in Cattle

  • Chung-Boo Kang;Woo-Song Ha;Ji-In Kwon;Young-Sang Yu;Chul-Ho Kim;Soo-Dong Kwak
    • Biomedical Science Letters
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    • v.8 no.4
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    • pp.235-244
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    • 2002
  • This study was carried out to determine the blood and milk progesterone by enzyme-linked immunosorbent assay (ELISA), and milk urea nitrogen (MUN) in cows. MUN and protein concentration were determined using automated infared procedures. The optimum conditions of ELISA system was investigated including the first and second antibody titres, bound percent, and enzyme conjugate and also the factors on MUN and protein concentration by sampling procedures and addition of preservatives. Progesterone antibodies did not react to pregnenlone, testosterone, estrone, estradiol-l7$\beta$, aldosterone, cortisol, corticosterone and 11$\alpha$-dehydroxycortisone (DOC), but reacted with only progesterone. The intra and inter-assay coefficient of variation 4.5%, 6.1~9.4% when used of bovine serum. The morning, MUN concentration (17.6$\pm$2.8 mg/100 ml) in the 13 herds was similar to that of evening MUN concentration of the lactating cows from the same herd. A significant relationship between morning and evening milk samples of upper parameters was found r=0.93. Difference in MUN concentration with sampling procedures and using of preservatives were investigated.

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A Case Report of Polyarticular Type Juvenile Idiopathic Arthritis(JIA) Patient Ineffective Response to DMARDs and NSAIDs (항류마티스제(DMARDs)와 비스테로이드성 소염진통제(NSAIDs)에 반응하지 않는 다수관절형 소아기 류마티스 관절염 환아 증례 보고)

  • Yoo, Chang-Kil;Lee, Yun-Ju
    • The Journal of Pediatrics of Korean Medicine
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    • v.24 no.1
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    • pp.57-64
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    • 2010
  • Objectives This study is to examine case that has an significant result on treating Polyarticular Type Juvenile Idiopathic Arthritis(JIA) the patient who did not response to DMARDs and NSAIDs with oriental medicine. JIA is the most common form of persistent arthritis in children before age 16. The symptoms of JIA is pain and edema of joints, fever, lethargy, reduced physical activity, and poor appetite. But the symptoms vary. Methods The patient felt pain and had limitation of mobility on his joints, but the DMARDs and NSAIDs were not effective. Therefore, he was treated with oriental medicine and withdrew from DMARDs and NSAIDs. The patient took DMARDs and NSAIDs from May 2007 to November 2008(He did not took DMARDs and NSAIDs from December 2007 to June 2008), and took oriental medicine from November 2008 until now. Results The patient's pain and limited mobility on his joints, edema, morning stiffness, fatigue was gone, and could maintain the condition. He doesn't have DMARDs or any other western medicine anymore. Conclusions This study shows that a Polyarticular Type Juvenile Idiopathic Arthritis patient who had ineffective response to DMARDs and NSAIDs can reach to the clinical remission by oriental medicine treatment. His morning stiffness, fatigue, pain of joints, edema was gone and ESR was in normal range with Oriental medicine treatment.

Study on Characteristics of Wastewater Flow for Namyangju City (남양주시 하수발생특성 연구)

  • Hwang, Byung-Gi;Lee, Eui-Sang;Kim, Kyung-Won;Son, Jung-Eun
    • Journal of Environmental Impact Assessment
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    • v.12 no.2
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    • pp.73-86
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    • 2003
  • In this study, we performed 8 times of water quality sampling and continuous measurement of flow to identify wastewater flow characteristics for wastewater treatment basins of Namyangju city. The field survey has been conducted 6 times in dry weather period and 2 times in wet weather period. Each survey conducted 24-hr water quality sampling in the interval of 2-hr. From the analysis of flow data and water quality data, the following conclusions can be drawn. For Namyangju-1 station, which represents residential and commercial mixed area, flow rises early in the morning before office-going hour and moves up and down within narrow range, and lasted after office-leaving hour, resulting from continuous commercial activity. In case of Namyangju-5 station representing community and Namyangju- 6 station representing residential area, the flow immediately falls down after rising early in the morning and later rises again at the office-leaving hour, and arrives at peak flows around late evening. In the analysis of wastewater characteristics for weekdays and weekends, without landuse types the flow rate of weekends is higher than that of weekdays because it reflects population, being not contributed to generate wastewater during the working hours of weekdays, stay home and produce wastewater for weekends period.

Comparison of Breakfast and Nutritional Attitudes in Elementary School Students by Obesity Level in Chungnam Area (충남 일부지역 초등학생의 비만도에 따른 아침식사와 영양태도 비교)

  • Lee, Soen Yong;Lee, Je-Hyuk;Kim, Myung Hee
    • Journal of the Korean Society of Food Culture
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    • v.29 no.5
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    • pp.390-405
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    • 2014
  • The aim of this study was to investigate the actual conditions underlying frequency of eating breakfast and nutritional attitudes in elementary school students in Chungnam area. Students had the highest ratio for eating breakfast everyday and spent 10-20 minutes eating breakfast. Students went to bed at 10-11 PM and woke up at 7-7:30 AM in the morning on average. The biggest reason for skipping breakfast was insufficient time in the morning. Additionally, favorite breakfast menu of subjects was a Korean style meal. The number of elementary students that recognized necessity of nutritional education was highest among subjects. Favorite educational method of subjects was education by teachers. Additionally, number of female students that recognized effect of breakfast on health status was significantly higher than boy students. Ratio of eating breakfast everyday was dependent on BMI value. Most subjects preferred video learning materials as the main nutritional education method. Overweight subjects showed the highest ratio for eating with loss of willpower compared to other students when they skipped the breakfast. Female subjects ate a greater variety of foods compared to male students, and showed better nutritional attitudes towards a desirable dietary life than male subjects. To prevent undesirable food habits and improve intake of various nutrients, systematic nutrition education is required to regulate breakfast of elementary students.

A Study on Work Analysis and Productivity of Food Service in Elementary Schools using Work Sampling (Work Sampling 을 이용한 국민학교급식의 작업분석과 생산성에 관한 연구)

  • Ahn, Tae-Hyun;Lee, Sook-Young
    • Korean journal of food and cookery science
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    • v.8 no.3
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    • pp.323-332
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    • 1992
  • This study was carried out to investigate work analysis by use of work sampling and productivity of school feeding at three elementary schools in Seoul. The results were as knows: 1. In the mean work percent of dietitians The direct work functions showed the highest, as the mean work percent was 40.4% for me direct work functions, 29.9% for me indirect work functions and 29.8% for me delays. Among the direct work functions, routine clerical was 30.9%, and receiving was 9.5%. 2. In the mean work percent of bakers The direct work functions (73.5%) marked the highest. Among those, cooking was 41.8%, transportation was 15.9%, and cleaning was 8.5%. 3. In the mean work percent of workers The direct work functions (781%) were me highest. Among those, the decreasing order was cooking (32.3%), cleaning (27.8%), and transportation (13.8%). 4. Work percent per labor hour The work percent per labor hour of dietitians was higher in the morning than in me afternoon, while they continued to work even during the lunch time. Bakers usually worked in me morning, but rarely worked in me afternoon. Workers had lunch and break time after their food service. In me school aided by parents volunteers, the total work time of workers was reduced by an hour and their work percent in me afternoon was high. 5. The number of mea1s served per labor hour was 32.6 meals, and me labor time spent per served mea1 was 1.8 minutes.

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The Relations of Preschoolers' Cortisol Pattern at Home to Tendency of Internalizing Behavior (유아의 가정에서의 코티솔 패턴과 내면화 행동성향 간의 관계)

  • Lee, Young;Shin, Yee-Jin;Chung, Jee-Nha;Min, Sung-Hye;Min, Hyun-Suk;Lee, Kyung-Sook
    • Journal of the Korean Home Economics Association
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    • v.45 no.9
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    • pp.53-62
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    • 2007
  • The purposes of this study were to look at the patterns of Korean preschoolers' cortisol levels at home and to examine the relations of cortisol pattern to the preschooler's internalizing behavioral tendency with focused on behavioral inhibition and internalizing problematic behavior. The subjects of the study were forty 4-year old preschoolers(27 boys and 13 girls). Saliva was collected at home during the weekends, and cortisol was analyzed by Radioimmunoassay procedure. "Strange Peer Situation Experiment" developed by Rubin, Coplan, Fox and Calkins(1995) and revised by Rubin, Burgess and Hastings(2002) was used to measure the preschooler's behavior inhibition. Problematic behavior was measured through a "Korean Children's Behavior Checklist" (Oh, K. J., Lee, H. R., Hong, K. E., & Ha, E. H., 1997). The results were as follows: First, on average for a 4-year old, the level of cortisol was 0.22 in the morning, and 0.14 in the afternoon. There was a pattern that cortisol levels had declined in the afternoon. There was no difference in cortisol levels between genders. Secondly, there was a tendency that children of higher inhibition level at the lab showed the higher cortisol levels in the morning. However, this difference was not statistically significant. Thirdly, the higher the cortisol levels at home, the more showed internalizing behavior. Externalizing behavior was not correlated with the cortisol levels. It was concluded that cortisol may be related to children's internalizing behavior tendency.

Objective Classification of Fog Type and Analysis of Fog Characteristics Using Visibility Meter and Satellite Observation Data over South Korea (시정계와 위성 관측 자료를 활용한 남한 안개의 객관적인 유형 분류와 특성 분석)

  • Lee, Hyun-Kyoung;Suh, Myoung-Seok
    • Atmosphere
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    • v.29 no.5
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    • pp.639-658
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    • 2019
  • The classification of fog type and the characteristics of fog based on fog events over South Korea were investigated using a 3-year (2015~2017) visibility meter data. One-minute visibility meter data were used to identify fog with present weather codes and surface observation data. The concept of fog events was adopted for the better definition of fog properties and more objective classification through the detailed investigation of life cycle of fog. Decision tree method was used to classify the fog types and the final fog types were radiation fog, advection fog, precipitation fog, cloud base lowering fog and morning evaporation fog. We enhanced objectivity in classifying the types of fog by adding the satellite and the buoy observations to the conventional usage of AWS and ceilometer data. Radiation fog, the most common type in South Korea, frequently occurs in inland during autumn. A considerable number of advection fogs occur in island area in summer, especially in July. Precipitation fog accounts for more than a quarter of the total fog events and frequently occurs in islands and coastal areas. Cloud base lowering fog, classified using ceilometer, occurs occasionally for all areas but the occurrence rate is relatively high in east and west coastal area. Morning evaporation fog type is rarely observed in inland. The occurrence rate of thick fog with visibility less than 100 meters is amount to 21% of total fog events. Although advection fog develops into thick fog frequently, radiation fog shows the minimum visibility, in some cases.

A Comparison of open and Directed Teaching Styles on Creativity and Achievement in Mathematical Concepts of Nursery School Chidren (유아원 아동의 창의력과 수학개념의 성취에 대한 개방수업방식과 지시수업방식의 비교)

  • Lee, Myoung Cho
    • Korean Journal of Child Studies
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    • v.10 no.1
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    • pp.1-10
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    • 1989
  • This study compared the effects of open and directed teaching styles on creativity and mathematical achievement. The subjects were 32 three- and four-year-old children enrolled in the Home Economics Laboratory Nursery School at the University of Arkansas during the fall semester of 1987. In this study, the open teaching style was a child-oriented method of teaching with the help or guidance rather than the actual instruction of teacher, while the directed teaching style was a teacher-oriented method of teaching with actual instruction of the teacher. Forty-eight activities and materials relevant to mathematical concepts appropriately designed for the subjects were used. The nursery school children were divided into morning and afternoon groups. Utilizing a Latin square design, the children in the morning group were taught by the directed teaching style for four weeks followed by a three week period of no planned mathematical activities, then taught by the open teaching style for four weeks. The children in the afternoon group followed the same schedule except the open teaching style was first. At the end of the two four-week sessions of mathematics experiences Thinking Creatively in Action and Movement and selected items of Tests of Basic Experiences 2: Mathematics were administered. The scores of each of the two tests were analyzed using a t-test of dependent measures for the two teaching styles, the sex, and the age of the children. Children taught using the directed teaching style showed a significantly higher originality and mathematical achievement scores than those taught using the open teaching style. Differences for sex and age revealed that the directed teaching style was a significantly better method of instruction to foster the originality for boys and the mathematical achievement for four-year-old children.

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A Study on the Effects of Protain Intakes on the Adaptation of Shift Work (단백질 섭취량이 주야 교대근무의 적응에 미치는 영향)

  • 왕수경
    • Journal of the Korean Home Economics Association
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    • v.30 no.1
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    • pp.87-98
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    • 1992
  • This study was undertaken to investigate protein effects on the adaptation of shift work. Since biorhythms differ according to the time of day, an investigation of the change of metabolism during day-shift and night-shift was undertaken by dividing twenty 3-shift worker into two groups: dormitory diet group and milk & egg supplemented group. Between the two groups, estimations were made on the concentrations of serum protain, albumin, cholesterol and cortisol and the excretion of vanilymandelic acid(VMA), creatinine and nitrogen in urine. The results are summarized as follows: 1. Serum ingredients were gathered from workers at 6:00-7:00h and 14:00-15:00h on the day of their change from morning shift(6:00-14:00h) to night shift(22:00-6:00h). On examnation of the serum ingredient it was found that, in dormitory diet group only the concentrarion level of serum albumin showed a change of rhythm adapted to night work, and in the milk & egg supplemented group the concentration levels of serum protein, albumin and cholesterol all showed a change of rhythm adapted to night work. Serum cortisol of both groups showed the same rhythm as during morining shfits, which implied nonadatation to night work, but the concentration was observed to be lower. 2. Excretion of urinary VMA and nitrogen were higher during night work than during morning work: they were also higher on the 6th day than on the 1st, in comparing the two groups, excretion of VMA was higher among the dormitory diet group, whereas the opposite was ture for excretion of nitrogen among the milk & egg supplemented group. There was no sign of difference in excretion of cretinine among the two experiment groups.

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