• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.3
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• pp.396-401
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• 2016

This study was performed to investigate the effects of various thermal treatments on the growth of Morganella morganii and Photobacterium phosphoreum and activity of crude histidine decarboxylase (HDC) obtained from M. morganii and P. phosphoreum. Crude HDC and the two strains were treated at $65^{\circ}C$/30 min, $80^{\circ}C$/10 min, $100^{\circ}C$/10 min, and $121^{\circ}C$/10 min. Activity of crude HDC decreased with increasing temperature. Viable cells counts of M. morganii and P. phosphoreum were not detected in any heated samples. SDS-PAGE patterns of heated HDC did not show significant differences up to $100^{\circ}C$. However, at $121^{\circ}C$, protein band intensity was weakened. In native-PAGE, there was a major change in the pattern of HDC at $65^{\circ}C$. These results suggest that thermal treatment can help to reduce histamine production by reducing HDC activity and growth of M. morganii and P. phosphoreum.

• Journal of fish pathology
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• v.32 no.2
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• pp.97-104
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• 2019

The purpose of this study was to investigate the antimicrobial resistance and resistance transfer of Vibrio parahaemolyticus and Morganella morganii isolated from fish products purchased from fish markets in Yeosu April - December 2017. These bacteria were identified by biochemical test and PCR results, and the transfer of antimicrobial resistance was confirmed by the broth mating method. To isolate the transconjugants formed during conjugation, TSA medium containing 50 ㎍/ml of ampicillin (AMP), and 150 ㎍/ml of streptomycin (SM) or 30 ㎍/ml of oxytetracycline (OT) was used. M. morganii isolates showed low susceptibility to AMP, amoxicillin (AML), and colistin (CT), erythromycin, OT, and tetracycline, compared to V. parahaemolyticus resistance to AMP, AML, and CT. The conjugation of V. parahaemolyticus or M. morganii with Escherichia coli resulted in the separation of V. parahaemolyticus and M. morganii showing SM resistance as transconjugants. Meanwhile, Edwardsiella tarda transconjugants showing AMP and AML resistance were obtained from the broth mating of V. parahaemolyticus and E. tarda. But the transfer of the VPA0477 which is a β-lactamase gene of V. parahaemolyticus was not confirmed. These results suggest that resistance transfer between pathogenic bacteria is bidirectional and progresses in a wide variety of patterns.

• BMB Reports
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• v.34 no.5
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• pp.415-420
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• 2001

Chloramphenicol acetyltransferase (CAT) was purified to homogeneity from Morganella morganii starting with ammonium sulphate fractionation, followed by separation on DEAE-Sephadex A50, and G-100 Sephadex gel filtration. The enzyme was purified 133.3 fold and showed a final specific activity of 60 units/mg protein with a yield of 37%. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of the purified enzyme revealed it as a heterotetramer that consists of four subunits with close molecular weights (19.5, 19, 18, and 17.5 kDa). The molecular weight of the native enzyme was calculated to be 78 kDa, as determined by gel filtration, which approximated to that of the four subunits (74 kDa). The enzyme showed a maximum activity at pH 7.8 when incubated at $35^{\circ}C$. A Lineweaver-Burk analysis gave a Km of 5.0 uM and Vmax of 153.8 U/ml. The amino acid composition of the purified enzyme was also determined.

• Journal of Veterinary Clinics
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• v.26 no.4
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• pp.391-394
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• 2009

An Asian water monitor Varanus salvator with physical wound due to bite which was subsequently infected with bacterium resulting to hemorrhage and pus in the skin blisters, abdominal distention and septicemia. Morganella morganii was isolated and identified from the blood and kidney of the reptile, and confirmed by PCR and biochemical tests. The sensitivity of isolated strains to different groups of antibiotics was also evaluated using the disc diffusion method. Pathogenicity test using M. morganii (SNUFPC-MM01) (1.6 ${\times}$ $10^{11}$CFU/mouse) to suckling and adult mice resulted to the death of all mice. This paper describes the first isolation of M. morganii from Asian water monitor in Korea.

• Journal of Microbiology and Biotechnology
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• v.24 no.4
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• pp.465-474
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• 2014

This study was performed to investigate the inhibitory effects of brown algae extracts on histamine production in mackerel muscle. First, antimicrobial activities of brown algae extracts against Morganella morganii were investigated using a disk diffusion method. An ethanol extract of Ecklonia cava (ECEE) exhibited strong antimicrobial activity. The minimum inhibitory concentration (MIC) of ECEE was 2 mg/ml. Furthermore, the brown algae extracts were examined for their ability to inhibit crude histidine decarboxylase (HDC) of M. morganii. The ethanol extract of Eisenia bicyclis (EBEE) and ECEE exhibited significant inhibitory activities (19.82% and 33.79%, respectively) at a concentration of 1 mg/ml. To obtain the phlorotannin dieckol, ECEE and EBEE were subjected to liquid-liquid extraction, silica gel column chromatography, and HPLC. Dieckol exhibited substantial inhibitory activity with an $IC_{50}$ value of 0.61 mg/ml, and exhibited competitive inhibition. These extracts were also tested on mackerel muscle. The viable cell counts and histamine production in mackerel muscle inoculated with M. morganii treated with ${\geq}2.5 $ MIC of ECEE (weight basis) were highly inhibited compared with the untreated sample. Furthermore, treatment of crude HD-Cinoculated mackerel muscle with 0.5% ECEE and 0.5% EBEE (weight basis), which exhibited excellent inhibitory activities against crude HDC, reduced the overall histamine production by 46.29% and 56.89%, respectively, compared with the untreated sample. Thus, these inhibitory effects of ECEE and EBEE should be helpful in enhancing the safety of mackerel by suppressing histamine production in this fish species.

• Journal of Veterinary Clinics
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• v.37 no.6
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• pp.342-344
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• 2020

Bacterial osteomyelitis-or bacterial infection of the bone-is common in reptiles. Unfortunately, its treatment is challenging despite advances in diagnostic and medical technologies. Herein, we present the case of a sexually mature female bearded dragon (Pogona vitticeps) with left forelimb elbow joint stiffness. We diagnosed the reptile with a eft elbow joint traumatic structural abnormality based on gross examination and evaluation of radiographs. Treatment with clindamycin and cephalexin for bacterial infection failed and the reptile died. Necropsy revealed the causative bacteria as Morganella morganii. Treatment of osteomyelitis is typically focused against Staphylococcus aureus as it the most common cause of traumatic bone infection. However, M. morganii, the causative bacterium in this case, has a natural resistance to clindamycin and cephalexin. Recently, these bacteria have begun to appear in clinical reports, more commonly as the causative organisms of bone infections. M. morganii should be considered as a potential cause of infection. Furthermore, antibiotic treatment in such cases should be based on bacterial culture and susceptibility tests.

• Childhood Kidney Diseases
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• v.25 no.2
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• pp.112-116
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• 2021

Hyperammonemia is mainly caused by diseases related to liver failure. However, there are also non-hepatic causes of hyperammonemia, such as urinary tract infection (UTI) due to urease-producing organisms. Urease production by these bacteria induces a hydrolysis of urinary urea into ammonia that can cross the urothelial cell membrane and diffuse into blood vessels, leading to hyperammonemia. Delayed diagnosis and treatment of hyperammonemia can lead to lethal encephalopathy that can cause brain damage and life-threatening conditions. In the presence of obstructive uropathy, UTI by urease-producing bacteria can lead to more severe hyperammonemia due to enhanced resorption of ammonia into the systemic circulation. In this report, we present a case of acute severe hyperammonemic encephalopathy leading to brain death due to accumulation of ammonia in blood caused by Morganella morganii UTI in a 10-year-old girl with cloacal anomaly, causing obstructive uropathy even after multiple corrections.

• Korean Journal of Clinical Laboratory Science
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• v.48 no.2
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• pp.124-129
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• 2016

Endotoxin, also known as lipopolysaccharide (LPS) produced by the cell wall of gram negative bacteria can be present in any liquid or on any biomaterial. Endotoxin in blood can cause fever and inflammation. In this study, we compared bacterial endotoxin using Escherichia coli O157:H7, Klebsiella oxytoca, Salmonella Typhi, Shigella sonnei and Morganella morganii. Bacteria were cultured for use in the experiment, and diluted to $1.5{\times}10^8CFU/mL$. A check marked sensitivity confirmatory test of the Limulus amebocyte lysate (LAL) reagent was performed to examine the validity. The end point reaction to each bacteria sample was confirmed with 10 fold dilution and then the final reaction end point was confirmed by 2 fold dilution between the dilution step and the upper dilution step. According to the results, in detection of endotoxins in more than 0.015 EU/mL, E. coli O157 was 75~37.5 CFU/mL, K. oxytoca 37.5~18.75 CFU/mL, M. morganii and S. Typhi 3.75~1.875 CFU/mL, and S. sonnei 7.5~3.75 CFU/mL. The resulting value was finally ensured by a confirmation test for the inhibitory factor. Based on this study, conduct of further research on bacterial endotoxin is encouraged.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1993.04a
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• pp.106-106
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• 1993

DWC-751은 그람양성 및 음성균주에 대하여 광범위한 항균스펙트럼을 가지는 것으로 나타났다. 그람양성균 S. aureus에 대하여는 cefpirome과 동등하며, cefotaxime 보다 4배, ceftazidime보다 16배 우수하였고 그람음성균에 대하여 DWC-751의 항균력은 cefpirome, cefotaxime보다 2배, ceftazidime보다 4-8배 우수하였다. Ps. aeruginosa에 대한 DWC-751의 항균력은 ceftazidime과 거의 동등한 항균력을 나타내었고, cefpirome보다 2배, cefotaxime보다 4-8배 우수한 항균력을 나타내었다. 임상분리균주 및 ofloxacin 내성균주에 대한 DWC-751의 항균력은 표준균주에 대한 결과와 같이 대조약물보다 우수하였다. 전신감염치료효과에 있어서 Streptococcus pyogenes, Serratia marcescens, Acinetobacter calcoaceticus, Morganella morganii, Proteus mirabilis에 대한 동물실험 결과, ED$_{50}$치에 의한 효능은 cefotaxime 보다 우수하였으며,Enterobacter cloacae, Pseudomonas aeruginosa에 대하여는 ceftazidime과 거의 동등하였다.

• Journal of Veterinary Clinics
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• v.37 no.1
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• pp.57-59
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• 2020

A 3-year-old, captive female river cooter was presented with a 4-day history of progressive unilateral swelling of the right side of the head, lethargy, and anorexia. History, physical examination, and radiographic examination revealed an aural abscess. After administration of antibiotics and supportive care, surgical intervention was performed. Swab samples were collected from the tympanic cavity during surgery for cytology and antimicrobial susceptibility testing. Molecular analyses of 16S ribosomal RNA gene sequences identified Citrobacter spp. and Morganella morganii. The patient was treated with ciprofloxacin and meloxicam and recovered after 2 months. This report describes the successful correction of a unilateral aural abscess that responded well to surgical intervention and a properly selected antibiotic.