• Title/Summary/Keyword: Montenegro

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Viruses Associated with Fig Mosaic Disease in Different Fig Varieties in Montenegro

  • Latinovic, Jelena;Radisek, Sebastjan;Bajceta, Milija;Jakse, Jernej;Latinovic, Nedeljko
    • The Plant Pathology Journal
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    • v.35 no.1
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    • pp.32-40
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    • 2019
  • Symptoms of fig mosaic disease have been noticed on leaves of fig (Ficus carica) for several decades, in Montenegro. In 2014, leaf samples were collected from trees of six fig cultivars in a plantation located in the main fig-producing area of Montenegro, to study the disease. After RNA isolation, samples were tested by RT-PCR for detection of nine fig viruses and three viroids. Four viruses were detected: fig leaf mottle-associated virus 1 (FLMaV-1), fig mosaic virus (FMV), fig mild mottle-associated-virus (FMMaV) and fig badnavirus 1 (FBV-1). Most of the viruses were present in mixed infections. The amplicons of the viruses were directly sequenced from both directions. A BLAST search of these sequences revealed sequence identities with their closest counterparts at GenBank of 92, 97, 92 and 100%, for FLMaV-1, FMV, FMMaV and FBV-1, respectively. Different responses in symptom expression due to the various virus combinations detected have been demonstrated. Variety $Su{\check{s}}ilica$ had the least symptom expression, with only one virus (FBV-1) found. Considering that the production of figs in Montenegro is increasing and has a substantial relevance in this geographic location, the results indicate that more attention should be given to improving the phytosanitary condition of fig trees in the country.

Characterization of Pseudomonas syringae pv. syringae, Causal Agent of Citrus Blast of Mandarin in Montenegro

  • Ivanovic, Zarko;Perovic, Tatjana;Popovic, Tatjana;Blagojevic, Jovana;Trkulja, Nenad;Hrncic, Snjezana
    • The Plant Pathology Journal
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    • v.33 no.1
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    • pp.21-33
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    • 2017
  • Citrus blast caused by bacterium Pseudomonas syringae is a very important disease of citrus occuring in many areas of the world, but with few data about genetic structure of the pathogen involved. Considering the above fact, this study reports genetic characterization of 43 P. syringae isolates obtained from plant tissue displaying citrus blast symptoms on mandarin (Citrus reticulata) in Montenegro, using multilocus sequence analysis of gyrB, rpoD, and gap1 gene sequences. Gene sequences from a collection of 54 reference pathotype strains of P. syringae from the Plant Associated and Environmental Microbes Database (PAMDB) was used to establish a genetic relationship with our isolates obtained from mandarin. Phylogenetic analyses of gyrB, rpoD, and gap1 gene sequences showed that P. syringae pv. syringae causes citrus blast in mandarin in Montenegro, and belongs to genomospecies 1. Genetic homogeneity of isolates suggested that the Montenegrian population might be clonal which indicates a possible common source of infection. These findings may assist in further epidemiological studies of this pathogen and for determining mandarin breeding strategies for P. syringae control.

Optimal intensity measures for probabilistic seismic demand models of RC high-rise buildings

  • Pejovic, Jelena R.;Serdar, Nina N.;Pejovic, Radenko R.
    • Earthquakes and Structures
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    • v.13 no.3
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    • pp.221-230
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    • 2017
  • One of the important phases of probabilistic performance-based methodology is establishing appropriate probabilistic seismic demand models (PSDMs). These demand models relate ground motion intensity measures (IMs) to demand measures (DMs). The objective of this paper is selection of the optimal IMs in probabilistic seismic demand analysis (PSDA) of the RC high-rise buildings. In selection process features such as: efficiency, practically, proficiency and sufficiency are considered. RC high-rise buildings with core wall structural system are selected as a case study building class with the three characteristic heights: 20-storey, 30-storey and 40-storey. In order to determine the most optimal IMs, 720 nonlinear time-history analyses are conducted for 60 ground motion records with a wide range of magnitudes and distances to source, and for various soil types, thus taking into account uncertainties during ground motion selection. The non-linear 3D models of the case study buildings are constructed. A detailed regression analysis and statistical processing of results are performed and appropriate PSDMs for the RC high-rise building are derived. Analyzing a large number of results it are adopted conclusions on the optimality of individual ground motion IMs for the RC high-rise building.

Novel optimal intensity measures for probabilistic seismic analysis of RC high-rise buildings with core

  • Pejovic, Jelena R.;Serdar, Nina N.;Pejovic, Radenko R.
    • Earthquakes and Structures
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    • v.15 no.4
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    • pp.443-452
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    • 2018
  • In this paper the new intensity measures (IMs) for probabilistic seismic analysis of RC high-rise buildings with core wall structural system are proposed. The existing IMs are analysed and the new optimal ones are presented. The newly proposed IMs are based on the existing ones which: 1) comprise a wider range of frequency velocity spectrum content and 2) are defined as the integral along the velocity spectrum. In analysis characteristics of optimal IMs such as: efficiency, practicality, proficiency and sufficiency are considered. As prototype buildings, RC high-rise buildings with core wall structural system and with characteristic heights: 20-storey, 30-storey and 40-storey, are selected. The non-linear 3D models of the prototype buildings are constructed. 720 non-linear time-history analyses are conducted for 60 ground motion records with a wide range of magnitudes, distances to source and various soil types. Statistical processing of results and detailed regression analysis are performed and appropriate demand models which relate IMs to demand measures (DMs), are obtained. The conducted analysis has shown that the newly proposed IMs can efficiently predict the DMs with minimum dispersion and satisfactory practicality as compared to the other commonly used IMs (e.g., PGA and $S_a(T_1)$). The newly proposed IMs overcome difficulties in calculating of integral along the velocity spectrum and present adequate replacement for IMs which comprise a wider range of frequency velocity spectrum content.

Probabilistic estimates of corrosion rate of fuel tank structures of aging bulk carriers

  • Ivosevic, Spiro;Mestrovic, Romeo;Kovac, Natasa
    • International Journal of Naval Architecture and Ocean Engineering
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    • v.11 no.1
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    • pp.165-177
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    • 2019
  • This paper considers corrosion wastage of two ship hull structure members as a part of investigated fuel oil tanks of 25 aging bulk carriers. Taking into account that many factors which influence corrosion wastage of ship hull structures are of uncertain nature, the related corrosion rate ($c_1$) is considered here as a real-valued continuous distribution, assuming that the corrosion wastage starts after 5, 6 or 7 years. In all considered cases, by using available data and applying three basic statistical tests, it is established that between two-parameter continuous distributions, normal, Weibull and logistic distributions are best fitted distributions for the mentioned corrosion rate ($c_1$). Note that the presented statistical, numerical and graphical results concerning two mentioned ship hull structure members allow to compare and discuss the corresponding probabilistic estimates for the corrosion rate ($c_1$).

WEAKLY DENSE IDEALS IN PRIVALOV SPACES OF HOLOMORPHIC FUNCTIONS

  • Mestrovic, Romeo;Pavicevic, Zarko
    • Journal of the Korean Mathematical Society
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    • v.48 no.2
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    • pp.397-420
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    • 2011
  • In this paper we study the structure of closed weakly dense ideals in Privalov spaces $N^p$ (1 < p < $\infty$) of holomorphic functions on the disk $\mathbb{D}$ : |z| < 1. The space $N^p$ with the topology given by Stoll's metric [21] becomes an F-algebra. N. Mochizuki [16] proved that a closed ideal in $N^p$ is a principal ideal generated by an inner function. Consequently, a closed subspace E of $N^p$ is invariant under multiplication by z if and only if it has the form $IN^p$ for some inner function I. We prove that if $\cal{M}$ is a closed ideal in $N^p$ that is dense in the weak topology of $N^p$, then $\cal{M}$ is generated by a singular inner function. On the other hand, if $S_{\mu}$ is a singular inner function whose associated singular measure $\mu$ has the modulus of continuity $O(t^{(p-1)/p})$, then we prove that the ideal $S_{\mu}N^p$ is weakly dense in $N^p$. Consequently, for such singular inner function $S_{\mu}$, the quotient space $N^p/S_{\mu}N^p$ is an F-space with trivial dual, and hence $N^p$ does not have the separation property.

Different Way of LMP/TAP/MHC Gene Clustering in Vertebrates,. Viviparity and Anti-tumor Immunity Failure

  • Bubanovic, Ivan;Najman, Stevo
    • Animal cells and systems
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    • v.9 no.1
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    • pp.1-7
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    • 2005
  • Class I and class II MHC genes have been identified in most of the jawed vertebrate taxa. In all investigated bony fish species, unlike mammals, the classical class I and class II MHC genes are not linked and even are found on different chromosomes. Linking and clustering of the class I and class II MHC genes is not the only phenomenon clearly detected in the evolution of immune system from cartilaginous to mammals. In all non-mammalian classes the LMP/TAP genes are highly conserved within class I genes region, while these genes are conserved within class II genes region only in mammals. Today we know that LMP/TAP genes in mammals have a crucial role in peptide processing for presentation within class I molecules, as well as in anti-tumor immunity. For these reasons, differences in clustering of LMP/TAP/MHC genes can be responsible for the differences in mechanisms and efficacy of anti-tumor immunity in non-mammalian vertebrates compared to same mechanisms in mammals. Also, the differences in cytokine network and anti-tumor antigens presentation within classes of vertebrates can be explained by toe peculiarity of LMP/TAP/MHC gene clustering.

Seismic evaluation of isolated skewed bridges using fragility function methodology

  • Bayat, M.;Daneshjoo, F.;Nistico, N.;Pejovic, J.
    • Computers and Concrete
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    • v.20 no.4
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    • pp.419-427
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    • 2017
  • A methodology, based on fragility functions, is proposed to evaluate the seismic performance of seismic isolated $45^{\circ}$ skewed concrete bridge: 1) twelve types of seismic isolation devices are considered based on two different design parameters 2) fragility functions of a three-span bridge with and without seismic isolation devices are analytically evaluated based on 3D nonlinear incremental dynamic analyses which seismic input consists of 20 selected ground motions. The optimum combinations of isolation device design parameters are identified comparing, for different limit states, the performance of 1) the Seismic Isolated Bridges (SIB) and 2) Not Seismic Isolated Bridge (NSIB) designed according to the AASHTO standards.

Optimization of a four-bar mechanism cyclic pitch control for a vertical axis wind turbine

  • Montenegro-Montero, Mariana;Richmond-Navarro, Gustavo;Casanova-Treto, Pedro
    • Wind and Structures
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    • v.35 no.2
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    • pp.121-130
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    • 2022
  • In this paper, the issue of pitch control in a vertical axis wind turbine was tackled. Programming the Actuator Cylinder model in MATLAB, a theoretical optimum pitch solution was found and then a classic four-bar mechanism was adapted to that theoretical solution to achieve a simple and elegant control of the pitch in the turbine. A simulation using the mechanism worked to find the optimum pitch cycles, where it was found that the mechanism would, in fact, increase the efficiency of the VAWT, by at least 11% and in the best case, over 35%. Another aspect that is studied is the possibility of self-start of the turbine by only changing the pitch on the blades. This analysis, however, proved that a further individual pitch control must be used to surpass the cogging torque. All analyses conducted were done for a specific wind turbine that is 2 m2 in the swept area.