• Toxicological Research
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• v.23 no.2
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• pp.159-164
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• 2007

Toxicity screening of a newly developed oral heparin derivative were carried out in 6 male cynomolgus monkeys (Macaca fascicularis), composed of a treatment group and vehicle control group. A newly orally active heparin derivative, developed by Seoul National University, was once given to treatment group at dose of 500 mg/kg. A treatment group did not show any change in body weights, hematological parameters including platelet-related varivables (platelet, PDW, PCT, MPV) and serum biochemical parameters (e.g., AST, ALT, BUN, etc.) for 2 weeks compared with those of vehicle control group. We also confirmed the maximum plasma concentration (Cmax, 1.73 IU/ml) and the time (Tmax, 1 hr) to reach Cmax. The present study will be valuable in the proper interpretation for nonclinical study using cynomolgus monkeys in the development of new drug of heparin derivative.

• Journal of Life Science
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• v.10 no.1
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• pp.37-39
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• 2000

Testis-specific protein Y(TSPY) is thought to play an important role during spermatogenesis in primates. The Arg and Lys-rich region of TSPY was implicated as a potential DNA binding site in the human and Japanese monkey. In the study, we investigated this further through looking at the putative amino acid sequences of Arg and Lys-rich region of TSPY gene from seven species of gibbons, five species of Old World monkeys and five species of New World monkeys. A comparison with those of the human and Japanese monkey revealed that the Arg and Lys-rich motif was hightly conserved in various primates. This finding suggests that a possible role for the Arg and Lys-rich motif in primate TSPY is DNA recognition. The functional implications for TSPY are discussed in the light of this and previous findings.

• Parasites, Hosts and Diseases
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• v.30 no.3
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• pp.177-182
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• 1992

Levels of platelets and other hematological values were monitored in 21 Saimiri and 12 Aotus monkeys over a period of three weeks post·infection with monkey·adapted Indochina CDC-1 strain of Plasmedium falciparum. In both Snlinoiri sciureus boliviensis and Aetus nancymai karyotype-1 monkeys the severest thrombocytopenia was observed at 14 days post-infection coinciding with peak parasitemia, neutropenia, Iynlphocytosis, and anemia associated with severe hemoglobinemia and elevated fibrinogen degeneration products(FDP's), MCH and MCV profiles in Aotus monkeys decreased with ascending parasitemia. In contrast, these parameters in Saimiri were characterized by a significant compensatory increase correlating with parasitemia. In general, thrombocytopenia was one of the earliest clinical manifestations of the infection with the platelets returning to normal levels shortly after peak parasitenlia at 14 days. Platelet kinetics had a strong correlation with hematologic and parasitologic values in the Aotus nlodel. No consistent associations were observed between platelet kinetics and other parameters in the Saimiri model. These data indicate that the Aotus model for malaria is more predictable than the Saimiri. Further, platelet turnover rates and recovery provide a useful prognostic parameter during malaria infection. The results are discussed in relation to the value of the two species of monkeys as models for the pathogenesis of human malaria.

• Proceedings of the Korean Society of Toxicology Conference
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• 2005.11a
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• pp.133-141
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• 2005

• Journal of Veterinary Clinics
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• v.17 no.1
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• pp.83-87
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• 2000

The mortalities of the monkeys that have been raised in Everland Zoological Gardens within a designated period (1976-1999) were retrospectively analyzed based on the clinical charts and/or autopsy reports. During that period, a total of 161monkeys from 8 species were died. Noninfectious and traumatic factors, respiratory and digestive disorders account for the majority of cause of death. 62% of the death was concentrated in the winter season. The mortality of female and male was 53 and 47%, respectively. The mortality of adult monkey(62%) due to neoplasia and senile changes.

• Proceedings of the Korean Society of Toxicology Conference
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• 2003.05a
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• pp.87-87
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• 2003

Various parameters of blood may be fluctuated by environmental factors such as transportation stress including diet, temperature, and so on). Thirty five 3- to 5 years-old cynomolgus monkeys were imported from Japan to evaluate potential toxic effect of a new biopharmaceutical. (omitted)

• Korean Journal of Veterinary Research
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• v.36 no.1
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• pp.161-167
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• 1996

Two cases of stomach mass and one case of tongue mass were observed in Japanese monkeys(Macaca fuscata) which were raised in Yong-in Farm Land. Histologically, two spontaneous tumors were found in the cardiac region of the stomach and were diagnosed as squamous cell carcinoma. These tumors had invaded the submucosa, muscular layer and serosa, but metastasis was not found in the other tissues including the lymph node. One spontaneous tongue mass was diagnosed as squamous cell carcinoma.

• Korean Journal of Veterinary Service
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• v.27 no.2
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• pp.165-169
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• 2004

In order to monitor the parasites, 83 fecal samples were taken from monkeys such as Cercopithecus mitis(6), Macaca fuscata(32), Saimiri sciurea(27), Macaca nigra(5), Papio hamadrysa(8), and Macaca mulatta(5) in Jeonbuk area. Isolation and Identification of the parasites from the feces were determined by the fecal examination using the floatation and microscopical examination, respectively. The detection rate was 21.6%, and mixed infection rate was single 18.0%(15 heads), double 2.4%(2 heads), and triple 1.2% (1 head). The isolates were identified as Strongyloides axei from 7 heads. Trichuris spp from 3 heads, Entamoeba histolytica 3 heads, Toxacaris leonina from 3 heads, and Eimeria spp from 2 heads.

• Journal of Veterinary Science
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• v.21 no.3
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• pp.48.1-48.18
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• 2020

Background: Mature oocytes at the metaphase II status (MII-stage oocytes) played an important role in assisted reproductive technology in non-human primates. Objectives: In order to improve the proportion of MII-stage oocytes retrieval, three different superovulation protocols were performed on 24 female cynomolgus monkeys. Methods: All the monkeys received once-daily injection of follicle-stimulating hormone (25 international unit [IU]) on day 3 of the menstruation, 3-day intervals, twice daily for 8-12 days until the time of human chorionic gonadotropin (1,500 IU) injection, on the 14-17th day of menstruation collecting oocytes. The difference between protocol I and protocol II was that 0.1 mg the gonadotropin-releasing hormone agonist was injected on day 1 of the menstruation, while the difference between personalized superovulation protocol and protocol II was that oocytes could be collected on the 14-17th day of menstrual cycle according to the length of each monkey. Results: The total number of oocytes harvested using the personalized superovulation protocol was much higher than that using protocol I (p < 0.05), and the proportion of MII-stage oocytes was significantly greater than that from either superovulation protocol I or II (p < 0.001 and p < 0.01 respectively), while the proportion of immature oocytes at the germinal vesicle was less than that from superovulation protocol I (p < 0.05). Conclusions: The personalized superovulation protocol could increase the rate of MII-stage oocytes acquired, and successfully develop into embryos after intracytoplasmic sperm injection, and eventually generated fetus.

• Toxicological Research
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• v.22 no.1
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• pp.61-67
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• 2006

Changes in hematology and serum biochemistry after treatment of recombinant human erythropoietin (rHuEPO, HM10760) were screened in 4 male cynomolgus monkeys (Macaca fascicularis). Four monkeys, composed of a treatment group of HM10760 and a positive control group of $Aranesp^(R}$, were subcutaneously administered at same dose of $100{\mu}g/kg$. Both groups did not show any change in body weights and food consumption for 4 weeks compared with those of pretreatment. Both groups did not show any change in total leukocyte count (WBC) and platelet count, while both groups showed increased platelet distribution width (PDW) percentage in HM10760 group during a period from day 5 to day 59 and in $Aranesp^(R}$ group during a period from day 9 to day 26. Both groups showed increases in red blood cells (RBC), hemoglobin (HGB), and hematocrit (HCT) approximately 10 days after treatment compared with those of pretreatment (day 0). The increased levels of RBC, HGB, and HCT were much higher in HM10760 than in $Aranesp^(R}$ by the increases of $3.2%{\sim}12.5%$ for RBC, $3.8%{\sim}17.1%$ for HCT, and $1.85%{\sim}11%$ for HGB. Both groups showed increases in red cells distribution width (RDW) and reticulocyte (RET) compared with those of pretreatment, showing the highest peak from day 9. The increased level of RET lasted up to day 14 in $Aranesp^(R}$ group, while it lasted up to day 23 in HM10760 group. The increased level of RDW lasted up to day 59, it was much higher in HM10760 by the increase of $10.1%{\sim}17.6%$ than in $Aranesp^(R}$ group. In serum biochemistry, both groups showed a decrease in chloride level compared with those of pretreatment. These findings indicated that HM10760 increased RBC, HGB, HCT, RDW, and RET compared with those of pretreatment, and the increased levels were much higher in HM10760 than in $Aranesp^(R}$.