• Title/Summary/Keyword: Moment arm

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DNA damages with Fpg/Endo Ⅲ FLARE Assay in cynomolgus monkeys exposed to stainless steel welding fume (용접흄 흡입노출 영장류에서 Fpg/Endo Ⅲ FLARE Assay를 이용한 DNA 손상 및 회복)

  • Rim, Kyung Taek;Kim, Soo Jin;Chung, Yong Hyun;Kim, Hyeon Yeong;Maeng, Seung Hee;Yu, Il Je
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.17 no.4
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    • pp.272-281
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    • 2007
  • 선박제조업을 비롯한 운송업 및 건축업 등의 다양한 분야에서 용접기술이 이용되어 옴에 따라 용접근로자들에 대한 산업보건학적 관심이 높아지고 있다. 노출정도가 다양하기는 하지만 용접흄은 6가 크롬을 비롯한 금속화합물과 유해가스, 화학물질 등을 복합적으로 포함하고 있는 스테인레스 스틸 용접흄에 대한 유전독성영향을 평가하기 위하여 흡입챔버를 이용, 실험동물인 영장류에 스테인레스 스틸 용접흄을 노출시키고 혈액 내 lymphocytes에 생성된 용접흄 노출농도 및 시간별 DNA 손상정도 및 그 회복효소를 측정함으로써, 유해성이 완전하게 확인되지 않은 용접흄에 노출되어 나타날 수 있는 암을 비롯한 심각한 건강영향을 예방하기 위한 각 지표들을 찾아 그 유용성을 비교하고자 하였다. 영장류를 노출시키기 위해 robotic arm을 장치한 영장류 흡입노출 시스템을 개발하였으며, 이 노출 시스템을 이용하여 수컷 영장류 6마리에 대해 용접흄 노출시험을 실시하였는데 실험군은 대조군 2, 저농도 ($31mg/m^3$) 노출군 2, 고농도 ($63mg/m^3$) 노출군 2마리로 구성하였고, 1일 2시간씩 일주일에 5일 동안 용접흄에 노출시켰다. 노출 농도는 지속적으로 모니터링 하였고, 노출과정 중에 영장류의 혈액을 채취하여 lymphocytes를 분리, 단세포 DNA 손상을 선별하기 위해 DNA 손상회복 효소인 E. coli formamidopyrimidine-DNA glycosylase (Fpg)와 endonuclease Ⅲ (Thymine Glycol-DNA glycosylase) 투여와 Comet asaay (single cell gel electrophoresis, 단세포겔전기영동기법)를 결합시켜 이용하는 Fpg/Endo III FLARE 분석법을 사용하였다. Fpg enzyme에 의한 olive tail moment값의 변화는 16주 노출군부터 노출부검(34주)군 까지 노출농도가 높아짐에 따른 olive tail moment 기하평균 값의 양 반응관계를 보기는 어렵지만, 고농도군의 경우 27주 노출군에서 가장 높은 olive tail moment 값을 보이고 이후 차츰 감소하였다. 한편 16주에서 22주까지의 노출기간에서는 대조군에 비해 노출군에서 DNA손상정도(olive tail moment값)는 모두 유의하게 높았으나, 6, 12, 18, 25, 31, 33, 35주간 노출하였을 때는 다른 결과를 보였다. 각 실험군의 Fpg enzyme에 의한 tail length값의 분포를 살펴볼 때, 저농도군 및 고농도군에서 27주간 노출하였을 때 가장 높은 tail length 값을 보이고 이후 차츰 감소하는 경향을 보였다. 또한 16, 22주간 노출하였을 때 대조군에 비해 노출군에서 tail length 값이 유의하게 높았으나, 20주간에서만 양 반응관계가 관찰되었고, 다른 주간에서는 양 반응 및 기간 반응관계를 나타내지는 않았다. Endo III enzyme에 의한 olive tail moment값의 변화는 기간별 노출군에서 대조군에 비해 높은 DNA손상정도(olive tail moment값)를 나타내는 결과들이 있었지만, 10, 12, 16, 22, 25, 31주간 노출하였을 때 등 상당수 노출기간에서 반응관계를 나타내지는 않았다. 각 실험군의 Endo III enzyme에 의한 tail length값의 분포를 살펴볼 때, 18, 20, 27, 33주간 노출하였을 때 대조군에 비해 노출군에서 tail length 값이 조금 높았지만, 양 반응 및 기간 반응관계를 보이지 않았고 수치의 크기가 불규칙하게 변화하였다. 즉, DNA에 있어 산화된 pyrimidine을 형성하여 손상된 부위의 염기를 제거함으로써 AP site (abasic site)를 만들고 이들이 Comet assay를 통해 break로 전환된 것을 포함한 DNA손상을 측정하기 위하여 endonuclease III (Endo III)를 첨가시킨 Endo III FLARE 분석법을 실시한 결과, 본 연구에서 나타난 결과는 용접흄 노출 영장류에서 Olive tail moment 및 tail length 공히 노출량 및 노출기간 반응관계를 볼 수 없었다. Endo III FLARE 분석법을 통한 산화적 DNA 손상지표는 영장류에 적용하기에는 적응반응현상으로 대조군과 유의한 차이도 관찰할 수 없었고 더욱이 역으로 대조군에서의 자연발생적 수치가 더 높아질 수 있어 용접흄 노출 영장류의 모니터링 지표로 사용하기에는 제한점이 있었다.

Development of Elbow Wearable Robot for Elderly Workers (고령층 근로자들을 위한 팔꿈치 착용형 로봇의 개발)

  • Lee, Seok-Hoon;Lee, Si-Haeng;Kim, Jung-Yup
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.39 no.6
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    • pp.617-624
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    • 2015
  • This paper describes the development of a wearable robot to assist the elbow muscle for use by elderly workers in aging societies. Various previously developed wearable robots have drawbacks in terms of their price, portability, and slow recognition of the wearer's intention. In this paper, emphasis is placed on the following features to minimize these drawbacks. The first feature is that an actuator is attached only at the elbow joint that withstands the highest moment during arm motion to reduce the weight, volume, and price of the robot and increase its practicality. The second is that operation of the wearable robot is divided into two modes, a tracking mode and a muscle strengthening mode, and the robot can automatically switch between these modes by analyzing the wearer's intention through the brachial muscle strength measuring device developed in this study. The assistive performance of the developed wearable robot is experimentally verified by motion tracking experiments without an external load and muscle strengthening experiments with an external load. During the muscle strengthening experiments, the power of the muscle of the upper arm is measured by a commercial electromyography (EMG) sensor. Motion tracking performance at a speed of $120^{\circ}/s$ and muscle assistance of over 60 % were obtained using our robot.

An Analytical Study on Strain Distribution Using Strain Gauge Attached On Root Surface (치근 부착 스트레인 게이지를 이용한 응력 분포 분석)

  • Kim, Sang-Cheol;Park, Kyu-Chan
    • The korean journal of orthodontics
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    • v.31 no.3 s.86
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    • pp.325-333
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    • 2001
  • Optimal orthodontic treatment could be possible when a orthodontist can predict and control tooth movement by applying a planned force system to the dentition. The moment to force(M/F) ratio at the bracket, has been shown to be a primary determinate of the pattern of tooth movement. As various n/F ratios are applied to the bracket on the tooth crown, strain distribution in periodontium can be changed, and the center of rotation in tooth movement can be determined. It is, therefore, so important in clinicalorthodontics to know the strain distribution in a force system of a M/F ratio. The purpose of this study was to analyze the strain distribution in orthodontic force system by strain gauge attached to tooth root, and to evaluate the usage of the method. For this study, an experimental upper anterior arch model was constructed, where upper central incisors, on the root surface of which, 8 strain gauges were attached, were implanted In the photoelastic resin, as in the case of 4mm midline diastema. Three types of closing of upper midline diastema closure were compared : 1. with elastomeric chain(100g force) in no arch wire, 2. elastomeric chain in .016“ round steel wire, 3. elastomeric chain in .016”x.022“ rectangular steel wire. The results were as follows. 1. Strain distributions on labial, lingual, mesial and distal root surface of tooth were able to be evaluated with the strain gauge method, and the patterns of tooth rotation were understood by presuming the location of moment arm. 2. Extrusion and tipping movement of tooth was seen in closing in no arch wire, and intrusion and bodily movement was seen with steel arch wire inserted.

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Biomechanical Analysis of Tsukahara Vault with Double Salto Backward Piked (도마 츠카하라 몸 접어 뒤로 두 번 돌기의 운동역학적 분석)

  • Lim, Kyu-Chan
    • Korean Journal of Applied Biomechanics
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    • v.14 no.3
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    • pp.135-147
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    • 2004
  • This study was conducted to investigate the technical factors of Lu Yu Fu vault actually performed by three men gymnasts participated in artistic gymnastics competition of 2003 summer Universiade in Daegu through the DLT method of three dimensional cinematography. To analyze these vaults, the instant events of Lu Yu Fu were set in the board touchdown(BTD), the board takeoff(BTO), the horse touchdown(HTD), the horse takeoff(HTO), the peak height(PH), the mat touchdown(MTD) respectively and the phases of that vault were set in the board contact(BC), the preflight(PRF), the horse contact(HC), the postflight(POF), the grounding on mat(GM) respectively After calculating the performance times, the CG displacement velocity, the kinetic energy impulse reaction force moment arm torque at the horse, the released angle piked angle addressed angle, the angular momentum angular velocity of whole body in x axis, and the horizontal displacement between the feet and CG, the following conclusions were reached. To perform the better Lu Yu Fu vault, a gymnast must have the large horizontal velocity of whole body with fast run-up, decrease the duration time and the horizontal vertical displacement of whole body in PRF, have the enough time to judge the correct magnitude and direction of force to brake or push the horse so as to lengthen the HC duration time at any cost. Also it is desirable to increase the horizontal vertical displacement of whole body in POF if possible, maintain the adequate piked position to decrease the angular velocity of whole body in x axis, prepare the grounding on mat previously and delay the release of the hand from the body to keep the angular momentum.

Study on Convergence Technique through Strength Analysis of Stabilizer Link by Type (스테빌라이저 링크의 종류별 강도 해석을 통한 융합 기술연구)

  • Cho, Jae-Ung
    • Journal of the Korea Convergence Society
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    • v.6 no.1
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    • pp.57-63
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    • 2015
  • In this study, the lower arm is connected and fixed at the model of the automotive stabilizer link as the moment is applied. There are models of 1, 2 and 3 as a length control type, a general type and a single body type respectively. These models are investigated by performing the convergence technique through the design and the strength analysis with CATIA and ANSYS. As the maximum equivalent stress of model 3 has the least, model 3 can endure the highest load among three models. As the fatigue analysis, model 3 has the minimum blocks as the frequency of stress state, model 3 becomes also safest among three models. As models of 1, 2 are in the order of the next safety, the number of blocks becomes larger as the frequency of stress state and the instability becomes higher. And it is possible to be grafted onto the convergence technique at design and show the esthetic sense.

A New Refined Truss Modeling for Shear-Critical RC Members (Part I) - lts derivation of Basic Concept - (전단이 지배하는 RC부재의 새로운 트러스 모델링 기법 연구 (전편) - 기본 개념 유도를 중심으로 -)

  • Kim Woo;Jeong Jae-Pyong;Kim Dae-Joong
    • Journal of the Korea Concrete Institute
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    • v.16 no.6 s.84
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    • pp.785-794
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    • 2004
  • This paper describes a new refined truss modeling technique derived based on the well-known relationship of V=dM/dx=zdT/dx+Tdz/dx in a reinforced concrete beam subjected to combined shear and moment loads. The core of the model is that a new perspective on the shear behavior can be gained by considering the variation of the internal arm length along the span, so that the shear resistance mechanism can be expressed by the sum of two base components; arch action and beam action. The sharing ratio of these two actions is determined by accounting for the compatibility of deformation associated to the two actions. Modified Compression Field Theory and the tension-stiffening effect formula in CEB/FIP MC-90 are employed in calculating the deformations. Then the base equation of V=dM/dx has been numerically duplicated to form a new refined truss model.

Design and performance test of a foot for a jointed leg type quadrupedal walking robot (관절형 4족 보행로봇용 발의 설계 및 성능시험)

  • Hong, Ye-Seon;Yi, Su-Yeong;Ryu, Si-Bok;Lee, Jong-Won
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.21 no.8
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    • pp.1250-1258
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    • 1997
  • This paper reports on the development of a new foot for a quadrupedal jointed-leg type walking robot. The foot has 2 toes, one at the front and the other at the rear side, for stable landing on uneven ground by point contact. The toes can move up and down independantly, guided by double-wishbone shaped parallel links which enable the lower leg to rotate with respect to a remote center on the ground surface. The motion of each toe is damped by a hydropneumatic shock absorber integrated in the foot in order to absorb the dynamic landing shock. Furthermore, the new foot can reduce the maximum hip joint drive torque by shortening the moment arm length between the hip joint and the landing force vector on the ground. Intensive experiments were carried out in this study by using a one-leg walking model to investigate the soft landing performance of the foot which could be hardly offered by conventional robot feet such as a flat plate with a gimbal type ankle joint. And it was confirmed that the hip joint torque of the leg walking on the flat surface could be reduced remarkably by using the new foot.

Biomechanical Testing of Anterior Cervical Spine Implants: Evaluation of Changes in Strength Characteristics and Metal Fatigue Resulting from Minimal Bending and Cyclic Loading

  • Kim, Sung-Bum;Bak, Koang-Hum;Cheong, Jin-Hwan;Kim, Jae-Min;Kim, Choong-Hyun;Oh, Seong-Hoon
    • Journal of Korean Neurosurgical Society
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    • v.37 no.3
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    • pp.217-222
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    • 2005
  • Objective: To achieve optimal fit of implant, it is necessary to bend the implant during spine surgery. Bending procedure may decrease stiffness of plate especially made of titanium and stainless steel. Typically titanium suffers adverse effects including early crack propagation when it is bent. We investigate whether 6 degree bending of titanium plates would decrease the stiffness after full cyclic loading by comparing with non-bending titanium plates group. Methods: Authors experimented 40 titanium alloy plates of 57mm in length, manufactured by 5 different companies. Total 40 plates were divided into two groups (20 bent plates for experimental group and 20 non-bent plates for control group). Twenty plates of experimental group were bent to 6 degree with 3-point bending technique and verified with image analyzer. Using the electron microscope, we sought for a initial crack before and after 3-point bending. Mechanical testing by means of 6000 cyclic axial-compression loading of 35N in compression with moment arm of 35mm-1.1 Nm was conducted on each plate and followed by the electron microscopic examination to detect crack or fissure on plates. Results: The stiffness was decreased after 6000 cyclic loading, but there was no statistically significant difference in stiffness between experimental and control group. There was no evidence of change in grain structure on the electron microscopic magnification. Conclusion: The titanium cervical plates can be bent to 6 degree without any crack or weakness of plate. We also assume that minimal bending may increase the resistance to fatigue fracture in cervical flexion-extension movement.

Biomechanical Properties of the Anterior Walker Dependent Gait of Patients with Knee Osteoarthritis (무릎관절 골관절염 환자의 보행기 보행에서 생역학적 특성)

  • Lee, In-Hee;Kwon, Gi-Hong;Park, Sang-Young
    • The Journal of Korean Physical Therapy
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    • v.25 no.5
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    • pp.239-245
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    • 2013
  • Purpose: Osteoarthritis occurs in many different joints of the body, causing pain, stiffness, and decreased function. The knee is the most frequently affected joint of the lower limb. The aim of this study was to investigate the differences of biomechanics between independent gait and anterior walker dependent gait of patients with osteoarthritis of the knee. Methods: Lower limb joint kinematics and kinetics were evaluated in 15 patients with knee osteoarthritis when walking independently and when walking with an anterior walker. Participants were evaluated in a gait laboratory, with self-selected gait speed and natural arm swing. Results: When walking with a dependent anterior walker, participants walked significantly faster (p<0.01), using a longer stride length (p<0.01), compared to independent gait. When walking with a dependent anterior walker, participants exhibited significantly greater knee flexion/extension motion (p<0.01) and lower knee flexion moment (p<0.05) compared to independent gait. When walking with a dependent anterior walker, participants showed significantly greater peak ankle motion (p<0.01), ankle dorsiflexion/plantarflexion moments (p<0.01), and ankle power generation (p<0.05) compared to independent gait. Conclusion: These biomechanical properties of gait, observed when participants walked with a dependent anterior walker, may be a compensatory response to impaired knee function to allow sufficient power generation for propulsion. Therefore, rehabilitative strategies for patients with osteoarthritis of the knee are needed in order to improve not only knee function but also hip and ankle function.

Hydrodynamic Evaluation for Developing the New Inflatable Kayak (신형 인플래터블 카약 개발을 위한 유체역학적 성능평가)

  • Hah, Chong-Ku;Kim, Ho;Lim, Lee-Young;Ki, Jae-Suk
    • Journal of Advanced Marine Engineering and Technology
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    • v.39 no.3
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    • pp.334-341
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    • 2015
  • This study is to evaluate hydrodynamic performance evaluation between three kinds of inflatable kayaks, that is, a frame kayak, a needle knife kayak, and a v-hull kayak. In order to test, inclining and turning trial test are performed in the Ocean Engineering Basin. Also, a resistance test is performed with a reduced scale kayak in the circulating water channel. Consequently, First, the moment arm of a v-hull kayak is the largest with 132.4mm, but turning radius of one was the smallest of them. Second, the resistance of a needle knife kayak is the smallest with 71N, the center of gravity of one was the lowest with 0.128m of them, and then needle knife kayak occurs in a draft overall. Consequently, the v-hull kayak has had the advantages on restoring force and turning performance than others. The needle knife kayak has been more excellent on resistance and center of gravity than others.