• Title/Summary/Keyword: Molecular weight cut-off (MWCO)

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Evaluation of Ultrafiltration Membrane Using Gel Permeation Chromatography (GPC를 이용한 한외여과막의 평가)

  • 정건용;정동진;김천호;신현수;민병렬;김래현
    • Membrane Journal
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    • v.13 no.2
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    • pp.81-87
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    • 2003
  • The permeation experiments f3r the aqueous dextran solutions of which the molecular weights were distributed between several thousands and million daltons were carried out using the dead-end cell system equipped with sheet membrane to determine the molecular weight cut-off (MWCO) of ultrafiltration membranes. The dextran rejections with respect to molecular weight were obtained by GPC analysis of feed and permeate solutions, then the MWCO was decided as the dextran molecular weight corresponding to the 90% rejection. The MWCO of PBTK membrane manufactured by Millipore was varied between 63,000 and 68,000 daltons as the pressure increased from 0.5 to 2.0 bar. However, the MWCO of PBQK(Millipore) and UE 1812(Saehan) under the above operating pressure increased to 3.5 and 4.3 times, respectively. Also, the MWCO of PBTK increased to 25% as the membrane permeation increased from 10 to 40% of the feed solution.

Effects of Surface Water Chemistry and Physicochemical Characteristics of Humic Acid on Fouling of Membrane (원수의 수질화학과 HA의 물리화학적 특성이 막 오염에 미치는 영향)

  • Bae, Jin-Youl;Han, Ihnsup;Park, Sung-Ho;Shin, Jee-Won
    • Journal of Korean Society on Water Environment
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    • v.21 no.3
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    • pp.242-247
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    • 2005
  • In this study, we investigated the removal efficiencies of pollutants and permeate fluxes depending on chemistry of feed water, various molecular weight cut-offs (MWCOs) and materials of membrane, operating pressure. We used seven MWCO membranes of YC0.5, YM1, YM3, YM10, YM30, YM100 and PM30, humic acid solution and surface water as feed water, and examined variation in permeate flux. Results of TOC removal experiment demonstrate that MWCO lower 1,000daltons could remove humic acid effectively. As increasing solution pH and decreasing divalent cations ($Ca^{2+}$) concentration, TOC removal increased. But $UV_{254}$ removal efficiency increased with higher divalent cation concentration and solution pH. Membrane fouling increased with increasing electrolyte (NaCl), divalent cation concentration and decreasing solution pH. In spite of initial permeate flux of the hydrophobic membrane (PM30) was higher than that of the hydrophilic membrane (YM30), flux decline of PM30 was significant during operation. At higher operating pressure, compactness of the cake layer on the membrane surface increased, resulting in gradual increase in hydraulic resistance.

Micellar Enhanced Ultrafiltration (MEUF) and Activated Carbon Fiber (ACF) Hybrid Processes for the Removal of Cadmium from an Aqueous Solution

  • Rafique, Rahman Faizur;Lee, Seunghwan
    • Korean Chemical Engineering Research
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    • v.52 no.6
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    • pp.775-780
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    • 2014
  • Micellar enhanced ultrafiltration (MEUF) was used to remove cadmium from an aqueous solution using sodium dodecyl sulfate (SDS) as a surfactant. Operational parameters such as initial permeate flux, retentate pressure, initial cadmium concentration, pH solution, molecular weight cut-off (MWCO), and molar ratio of cadmium to SDS were investigated. Removal efficiency of cadmium from an aqueous solution increased with an increase of retentate pressure, pH solution and molar ratio of cadmium to SDS, and decreased with an increase of initial permeate flux. Higher removal efficiency of cadmium from the aqueous solution was achieved using lower MWCO (smaller membrane pore size). Under optimized experimental condition, cadmium removal efficiency reached 74.6 % within an hour. Using MEUF-ACF hybrid process the removal efficiency of both cadmium and SDS was found to be over 90%.

Purification and Characterization of Angiotensin I Converting Enzyme lnhibitory Peptides from Enzymatic Hydrolysate of Cod Liver Protein (대구의 간 단백질의 효소적 가수분해물로부터 안지오텐신 I 전환효소 저해 펩타이드의 분리.정제 및 특성)

  • 최영일;박표잠;최정호;변희국;정인철;문성훈;김세권
    • Journal of Life Science
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    • v.10 no.2
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    • pp.140-149
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    • 2000
  • In order to utilize marine processing waste which would normally be discarded, cod liver protein was hydrolysed by ${\alpha}$-chymotrysin, and the hydrolysate was investigated for the new angiotensin I converting enzyme (ACE) inhibitor. Thy hydrolysate was separated into three major types, with molecular weight cut-off (MWCO) values less than 10 kDa, 5 kDa and 1 kDa of ultrafiltration membranes, respectively. ACE inhibitory peptides were isolated from the fractions passed through MWCO 1 kDa membrane, and purified by using ion-exchange chromatography on a SP-Sephadex C-25 column, gel filtration on a Sephadex G-15 column, and HPLC on an ODS column. The purity was identified with capillary electrophoresis. The amino acid sequences of two peptides were Met-Ile-Pro-Pro-Tyr-Tyr (IC50=10.9 ${\mu}$M) and Gly-Leu-Arg-Asn-Gly-Ile (IC50=35.0 ${\mu}$M)

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Fabrication and Characterization of Alumina Hollow Fiber Ultrafiltration Membrane (알루미나 중공사 한외여과막 제조 및 특성평가)

  • Kim, Yeo-Jin;Kim, Seong-Joong;Kim, Jeong;Cho, Young-Hoon;Park, Hosik;Lee, Pyung-Soo;Park, You-In;Park, Ho-Bum;Nam, Seung-Eun
    • Membrane Journal
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    • v.28 no.1
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    • pp.21-30
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    • 2018
  • In this study, homogeneous and defect-free ceramic ultrafiltration membranes were fabricated by using the sol-gel method. A boehmite sol was synthesized and coated onto the surface of alumina hollow fiber microfiltration membranes. The effect of sol viscosity and surface tension on the coating layer homogeneity and thickness was investigated. The optimum coating repetition using pristine sol was determined to be 3 times, as the samples coated more than 4 times showed delamination. Fixing the coating repetition to 3 times, the effect of sintering temperature was also studied in this work. The samples sintered at $1000^{\circ}C$ exhibited the highest pure water permeability with the molecular weight cut-off (MWCO) of approximately 51 kDa (10 nm dextran), and the samples sintered at 600, $800^{\circ}C$ displayed the MWCO of 12 kDa (5 nm dextran). The ultrafiltration membranes prepared in this work showed competitive performance compared to the reported ceramic ultrafiltration membranes.

Lake Water Treatment Using a Ultrafiltration Membrane Process of Hollow Fiber Type (중공사형 한외여과 막분리 공정에 의한 하천수 처리)

  • 박진용
    • Membrane Journal
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    • v.7 no.1
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    • pp.39-47
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    • 1997
  • The self-designed membrane system was tested to examine the performance of the hollow fiber type polysulfone ultrafiltration(UF) membrane for the treatment of pure water(the 3rd treated water). The molecular weight cut-off's (MWCO) of the membranes used in this study were 5, 000 and 10, 000, respectively. The recovery rate, the ratio of permeate flow rate to the feed flow rate, increased as the temperature rose. The values of MWCO obtained in this study, using 2, 000 ppm polyethylene glycol and dextran solutions with various molecular weight, showed higher values than those suggested by SKI. Based on the results of the primary experiments, the water of the Gongji-stream, in which water quality is deteriorated by the inflow of domestic wastewater, was selected for the UF membrane test. Biological oxygen demand(BOD), total solids, and turbidity of the treated water had much lower values than those of the source water. Therefore, this study confirmed the possibility of the domestic water treatment using the hollow fiber type UF membrane.

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Enzyme Deactivation During Enzyme Recycling with Ultrafiltration Hollow Fibers (한외여과막을 이용한 효소재순환 시스템에서의 효소역가감소)

  • 김준석;정용섭홍석인
    • KSBB Journal
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    • v.11 no.3
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    • pp.347-352
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    • 1996
  • The enzyme deactivation in a membrane recycling system for the simultaneous saccharification and fermentation(SSF) was studied under various temperature and pressure. The optimum molecular weight cut off(MWCO) of the ultrafiltration membrane for recycling cellulase and ${\beta}$-glucosidase was 50,000. When the cellulase was recycled continuously through the membrane system, it was not deactivated. But the activity of ${\beta}$-glucosidase was decreased with an increase in operating temperature and transmembrane pressure. After 720 minutes at $42^{\circ}C$ and 24.8 psig , the activity of ${\beta}$-glucosidase was reduced by 35% of the initial activity. Such tendencies could be well explained by the results of highly induced shear at the fiber surface of membrane when temperature and transmembrane pressure became higher.

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Drinking Water Treatment of Surface Water Using Microfiltration-Nanofiltration Processes (정밀여과 및 나노여과 공정을 이용한 지표수의 상수처리)

  • Lee, Sung-Woo;Kim, Chung-Han;Kwak, Dong-Heui
    • Journal of Korean Society of Water and Wastewater
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    • v.14 no.3
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    • pp.224-230
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    • 2000
  • Membrane processes are capable of removing much materials from water. The removal or rejection characteristics of a membrane is usually depend upon the nominal pore size or MWCO(molecular weight cut off). A membrane with a smaller nominal pore size or MWCO should be capable of removing smaller contaminants from water. A series of experiments was performed to investigate the separation characteristics of membrane processes which consisted of microfiltration(MF) and nanofiltration(NF). To evaluate removal efficiencies of some pollutants such as the consumption of $KMnO_4$, THMFP, NH3-N, Fe, Mn, and pesticides, source water sampled from the Kum river was treated by the those membrane processes. Also, the results of experiments were compared with those of conventional water treatment processes. By two types of the membrane process, total removal efficiency of $KMnO_4$ consumed, THMEP, and $NH_3-N$ were 91.0%, 84.3%, and 85.5%, respectively and those processes were efficient in pesticides removal as well. Most of the effluents satisfied the Korean standard of drinking water quality continuously in the experimental periods. However, NF was needed for producing the safe drinking water in case of treating the raw water contaminated with Mn since removal efficiency of MF was not high enough. On the basis of the experimental results, it was suggested that NF could be applied to remove not only $NH_3-N$ but THMFP even without pre-chlorination.

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Concentration of Fresh Gel from Aloe vera L. by Using Ultrafiltration Process (한외여과 공정에 의한 알로에 베라 겔 농축)

  • Baek, Jin-Hong;Kim, Sung-A;Lee, Shin-Young
    • KSBB Journal
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    • v.23 no.2
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    • pp.169-176
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    • 2008
  • The concentration of fresh gel from Aloe vera L. by using ulfrafiltration (UF) process was investigated and analyzed. The two membranes (organic and ceramic) with different molecular weight cut-off (MWCO) and modules (flat sheet and tubular) was used. Under optimum operation conditions, ceramic (zirconium dioxide) tubular membrane with MWCO of 50 kDa resulted in higher flux, less fouling, more turbid, higher total solid, higher polysaccharide and less aloin content. Optimum operation conditions were transmembrane pressure of 1.0 bar, feed velocity of 240 L/hr and temperature of $23^{\circ}C$. Volume concentration factor of aloe gel was 3.13 at permeate flux of $51.1\;L/m^2{\cdot}hr$ after processing time of 1.66 hr. Aloin in fresh aloe gel by UF process was effectively removed as permeate and bioactive polysaccharide content was 2.1 times higher than that of fresh aloe gel. These results allowed a very good level of concentration degree and polysaccharide content. Thus, ultrafiltration process of this study was suitable for the concentration of fresh aloe gel though the aloe concentrate showed both the viscosity decrease and partially separation of liquid layer during storage at $4^{\circ}C$.

Separation and Purification of Lysozyme from Chicken Eggwhite Through Ultrafiltration (한외여과를 통한 난백 중 라이소자임의 분리정제)

  • Koo Ja-Kyung;Son Dongho;Jun Hoejin;Lee Yunhee;Cho Namjun;Jang Dong Il
    • Membrane Journal
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    • v.15 no.2
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    • pp.121-131
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    • 2005
  • Separation and purification of lysozyme from chicken egg white was studied using ultrafiltration. We have obtained experimental data through the cellulose membranes with the molecular weight cut off (MWCO) of 10 kDa, 30 kDa and 100 kDa in a stirred ultrafiltration device. Certain amounts of egg white were dissolved into 20 mM phosphate buffers of pH 6, 7 and 8 to make protein solutions of $1\%,\;2\%,\;3\%\;and\;10\%$ concentration. Permeation flux increased with increasing MWCO of the membrane. Permeation flux increased with increasing transmembrane pressure (TMP) and decreasing the protein concentration. As the MWCO of membrane decreased, the selectivity increased. The selectivity increased with increasing TMP and protein concentration of the solution.