• Journal of Microbiology and Biotechnology
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• v.25 no.8
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• pp.1241-1245
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• 2015

The aim of the present work was to examine the putative promoter region of the operon ansPAB and to determine the general elements required for the regulation of transcriptional activity. The transcriptional start site of the ansPAB promoter was determined by using highresolution S1-nuclease mapping. Sequence analysis of this region showed -10 and -35 elements, which were consistent with consensus sequences for R. etli promoters that are recognized by the major form of RNA polymerase containing the σ⁷⁰ transcription factor. Mutation studies affecting several regions located upstream of the transcriptional start site confirmed the importance of these elements on transcriptional expression.

• The Plant Pathology Journal
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• v.17 no.2
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• pp.67-70
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• 2001

Medicago truncatula is a diploid legume plant related to the forage crop alfalfa. Recently, it has been chosen as a model species for genomic studies due to its small genome, self-fertility, short generation time, and high transformation efficiency. M. truncatula engages in symbiosis with nitrogen-fixing soil bacterium Rhizobium meliloti. M. truncatula mutants that are defective in nodulation and developmental processes have been generated. Some of these mutants exhibited altered phenotypes in symbiotic responses such as root hair deformation, expression of nodulin genes, and calcium spiking. Thus, the genes controlling these traits are likely to encode functions that are required for Nod-factor signal transduction pathways. To facilitate genome analysis and map-based cloning of symbiotic genes, a bacterial artificial chromosome library was constructed. An efficient polymerase chain reaction-based screening of the library was devised to fasten physical mapping of specific genomic regions. As a genomics approach, comparative mapping revealed high levels of macro- and microsynteny between M. truncatula and other legume genomes. Expressed sequence tags and microarray profiles reflecting the genetic and biochemical events associated with the development and environmental interactions of M. truncatula are assembled in the databases. Together, these genomics programs will help enrich our understanding of the legume biology.

• Biomolecules & Therapeutics
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• v.24 no.2
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• pp.191-198
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• 2016

The vitamin D receptor (VDR) is a member of the nuclear receptor (NR) superfamily. The VDR binds to active vitamin $D_3$ metabolites, which stimulates downstream transduction signaling involved in various physiological activities such as calcium homeostasis, bone mineralization, and cell differentiation. Quercetin is a widely distributed flavonoid in nature that is known to enhance transactivation of VDR target genes. However, the detailed molecular mechanism underlying VDR activation by quercetin is not well understood. We first demonstrated the interaction between quercetin and the VDR at the molecular level by using fluorescence quenching and saturation transfer difference (STD) NMR experiments. The dissociation constant ($K_d$) of quercetin and the VDR was $21.15{\pm}4.31{\mu}M$, and the mapping of quercetin subsites for VDR binding was performed using STD-NMR. The binding mode of quercetin was investigated by a docking study combined with molecular dynamics (MD) simulation. Quercetin might serve as a scaffold for the development of VDR modulators with selective biological activities.

• The Bulletin of The Korean Astronomical Society
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• v.43 no.1
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• pp.66.2-66.2
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• 2018

The probability distribution function of column density (N-PDF) has been used for studying the characteristics of molecular clouds. In particular, the properties of N-PDF can reveal the nature of turbulence and gravity inside the molecular cloud. We use the dust continuum emission at $450{\mu}m$ and $850{\mu}m$ observed as part of the JCMT Gould Belt Survey (GBS) (Mairs et al. 2016), the 12CO J=1-0 line observed with the 45 m telescope at Nobeyama Radio Observatory (NRO) (Shimajiri et al. 2011), 13CO, C18O and HCO+ J=1-0 observed with the 13.7 m telescope at Taeduk Radio Astronomy Observatory (TRAO), as part of the TRAO key science project, "mapping Turbulent properties In star-forming MolEcular clouds down to the Sonic scale" (TIMES; PI: Jeong-Eun Lee). We here present the N-PDFs derived from the continuum and the molecular line emission toward the Orion A molecular cloud and compare their behaviors in order to investigate the chemical and optical depth effects on the N-PDF.

• The Bulletin of The Korean Astronomical Society
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• v.44 no.1
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• pp.76.3-76.3
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• 2019

We present the results from our comparisons of HCN and HCO+ (J=4-3) with HI and $H_2$ gas in NGC 6946, a sample from a mapping study of the dense molecular gas in the strongest star-forming galaxies (MALATANG). The MALATANG is one of the JCMT legacy surveys on the nearest 23 IR-brightest galaxies beyond the Local Group, which aims to study the relations of dense molecular gas with more general cool gas such as atomic and molecular hydrogen gas, and star formation properties in active galaxies. In this work, we particularly focus on the comparisons between the JCMT HCN/HCO+ (J=4-3) data and the THINGS HI/the NRO CO (J=1-0) data. We probe the dense molecular gas mass as a function of HI and $H_2$ mass in different locations in the central ${\sim}1.5kpc^2$ region. We discuss how the excess/deficit of $HI/H_2$ or total cool gas ($HI+H_2$) mass controls the presence and/or the fraction of dense molecular gas.

• Bulletin of the Korean Chemical Society
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• v.32 no.12
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• pp.4281-4285
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• 2011

We introduced a promising patterned substrate by using a microcontact printing method that can be used for SERS immunoassays based on antigen-antibody binding. SERS spectrum of the Raman reporter with antibody, which is rhodamine 6G (R6G) adsorbed on colloidal gold nanoparticles, was observed only for the surfaces in which prostate-specific antigen (PSA) is present on the substrate that is attached to an immobilized layer of antibody on the gold nanoparticles layer of the patterned substrate. Raman mapping images clearly showed that the antibodies on the Raman reporter were successfully and selectively conjugated with the antigen on the patterned substrate. This method could be potentially extended to multi-protein detections and ultrasensitive biosensors.

• Publications of The Korean Astronomical Society
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• v.20 no.1 s.24
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• pp.1-5
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• 2005

We report molecular line observations of CO(1-0), $^{13}CO(1-0)$, CS(2-1), and HCN(1-0) with SRAO 6m telescope toward L1014-IRS which is thought to be a very faint infrared source embedded in previously known 'starless' core L1014. The CO(1-0) observations find several components with different velocities along the line of sight of L1014, $4km\;s^{-1}$ and between $40{\sim}50km\;s^{-1}$. We find a parsec scale CO molecular outflow at the $4km\;s^{-1}$ component for the first time the direction of which is coincident with that of the small scale (${\sim}500pc$) outflow previously found. Although the observation is not covered for whole area of the outflow, the size of the molecular outflow seems not very inconsistent with the expected age of L1014-IRS. More accurate size and shape of the molecular outflow from L1014-IRS will be determined from the full coverage mapping in CO over the outflow region in very near future.

• The Bulletin of The Korean Astronomical Society
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• v.42 no.1
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• pp.33.1-33.1
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• 2017

Molecular clouds are the sites of stellar birth, and conditions within the clouds control the mode and tempo of star formation. In particular, turbulence largely determines the density and velocity fields, and can affect the gas kinetic temperature as it decays via shocks. However, despite its central role in star formation and many years of study, the properties of turbulence remain poorly understood. As a part of the TRAO key science program, "Mapping turbulent properties of star-forming molecular clouds down to the sonic scale (PI: Jeong-Eun Lee)", we mapped the northern region of the Orion A molecular cloud and the L1688 region of the ${\rho}$ Ophiuchus molecular cloud in 2 sets of lines (13CO 1-0/C18O 1-0 and HCN 1-0/and HCO+ 1-0) using the Taeduk Radio Astronomy Observatory (TRAO) 14-m telescope. We analyze these maps using a python package 'Turbustat', a toolkit which contains 16 different turbulent statistics. We will present the preliminary results of our TRAO observations and various turbulence statistical analyses.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.12
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• pp.1665-1669
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• 2001

A QTL was localized near S0120 on porcine chromosome 18. The QTL was significant (p<0.05) for average daily gain (ADG) of body weight and backfat thickness (BFT). The estimates of additive and dominance effects for the QTL were 0.0135 kg/day (p<0.001) and 0.0138 kg/day (p>0.5) for ADG and 1.6115 mm (p<0.001) and 0.9281 mm (p>0.05) for BFT. The location of this QTL coincided with a few growth hormone pathway genes. This study suggested that a QTL allele probably resulted from a mutation responsible for physiological lipase deficiency favoring obesity. This QTL might be important to obesity as well as growth in pigs.

• Journal of The Korean Astronomical Society
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• v.39 no.1
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• pp.19-24
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• 2006

We carried out CO survey toward IR-excess clouds using SRAO 6-m telescope in search of molecular $H_2$. These clouds, which show far-infrared excess over what is expected from HI column density, are considered to be candidates of molecular clouds. In order to find new high Galactic latitude clouds, we made mapping observations for 14 IR-excess clouds selected from Reach et al.(1998) in $^{12}CO$ J = 1 - 0 line, supplementing the similar survey in southern hemisphere (Onishi et al. 2001). $^{12}CO$ emission is detected from three IR-excess clouds among 14 objects. Three newly detected clouds exhibit somewhat clumpy morphology and column densities amount to ${\sim}10^{21}\;cm^{-2}$. One of three clouds, DIR120-28, show discrepancy between IR-excess center and CO emission center. It seems that IR-excess may not be an effective tracer of molecular gas. Instead, optical depth$(\tau)$ excess, i.e., IR-excess corrected for temperature dependence, may be more effective tracer of molecular clouds, since, by combining statistics from both hemispheres, we found that the detection rate is higher for IR-excess clouds with lower dust temperature.