Browse > Article
http://dx.doi.org/10.5012/bkcs.2011.32.12.4281

SERS Immunoassay Using Microcontact Printing for Application of Sensitive Biosensors  

Hong, Won-Jin (Department of Chemistry, Institute for Molecular Science and Fusion Technology, Kangwon National University)
Seo, Hyeong-Kuyn (Department of Chemistry, Institute for Molecular Science and Fusion Technology, Kangwon National University)
Jung, Young-Mee (Department of Chemistry, Institute for Molecular Science and Fusion Technology, Kangwon National University)
Publication Information
Bulletin of the Korean Chemical Society / v.32, no.12, 2011 , pp. 4281-4285 More about this Journal
Abstract
We introduced a promising patterned substrate by using a microcontact printing method that can be used for SERS immunoassays based on antigen-antibody binding. SERS spectrum of the Raman reporter with antibody, which is rhodamine 6G (R6G) adsorbed on colloidal gold nanoparticles, was observed only for the surfaces in which prostate-specific antigen (PSA) is present on the substrate that is attached to an immobilized layer of antibody on the gold nanoparticles layer of the patterned substrate. Raman mapping images clearly showed that the antibodies on the Raman reporter were successfully and selectively conjugated with the antigen on the patterned substrate. This method could be potentially extended to multi-protein detections and ultrasensitive biosensors.
Keywords
Surface-enhanced raman scattering (SERS); Immunoassay; Microcontact printing; Gold nanopaticles; Biosensors;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 0  (Related Records In Web of Science)
Times Cited By SCOPUS : 0
연도 인용수 순위
1 Combs, Z. A.; Chang, S.; Clark, T.; Singamaneni, S.; Anderson, K. D.; Tsukruk, V. V. Langmuir 2011, 27, 3198.   DOI   ScienceOn
2 Galarreta, B. C.; Norton, P. R.; Lagugne-Labarthet, F. Langmuir 2011, 27, 1494.   DOI   ScienceOn
3 Vo-Dinh, T.; Wang, H.-N.; Scaffidi, J. J. Biophoton 2010, 3, 89.
4 Yoon, K. J.; Seo, H. K.; Hwang, H.; Pyo, D.; Eom, I.-Y.; Hahn, J. H.; Jung, Y. M. Bull. Korean Chem. Soc. 2010, 31, 1215.   과학기술학회마을   DOI   ScienceOn
5 Shen, A.; Chen, L.; Xie, W.; Hu, J.; Zeng, A.; Richards, R.; Hu, J. J. Adv. Funct. Mat. 2010, 20, 969.   DOI   ScienceOn
6 Xiao, N.; Yu, C. Anal. Chem. 2010, 82, 3659.   DOI   ScienceOn
7 Han, X. X.; Kitahama, Y.; Itoh, T.; Wang, C. X.; Zhao, B.; Ozaki, Y. Anal. Chem. 2009, 81, 3350.   DOI   ScienceOn
8 Wang, G.; Park, H.-Y.; Lipert, R. J. Anal. Chem. 2009, 81, 9643.   DOI   ScienceOn
9 Bao, F.; Yao, J.-L.; Gu, R.-A. Langmuir 2009, 25, 10782.   DOI   ScienceOn
10 Chon, H.; Lee, S.; Son, S. W.; Oh, C. H.; Choo, J. Anal. Chem. 2009, 81, 3029.   DOI   ScienceOn
11 Jehn, C.; Küstner, B.; Adam, P.; Marx, A.; Ströbel, P.; Schmuck, C.; Schlücker, S. Phys. Chem. Chem. Phys. 2009, 11, 7499.   DOI   ScienceOn
12 Wang, G.; Park, H.-Y.; Lipert, R. J. Anal. Chem. 2009, 81, 9643.   DOI   ScienceOn
13 Han, X. X.; Cai, L. J.; Guo, J.; Wang, C. X.; Ruan, W. D.; Han, W. Y.; Xu, W. Q.; Zhao, B.; Ozaki, Y. Anal. Chem. 2008, 80, 3020.   DOI   ScienceOn
14 Sun, L.; Yu, C.; Irudayaraj, J. Anal. Chem. 2008, 80, 3342.   DOI   ScienceOn
15 Kneipp, K.; Moskovits, M.; Kneipp, H. Surface-Enhanced Raman Scattering-Physics and Applications; Springer: Heidelberg and Berlin. 2006.
16 Aroca, R. Surface-Enhanced Vibrational Spectroscopy; John Wiley and Sons: Chichester; UK. 2006.
17 Schatz G. C.; Van Duyne, P. R. Handbook of Vibrational Spectroscopy Vol. 1: Electromagnetic Mechanism of Surface-Enhanced Spectroscopy, Chalmers, UK. J. M. 2002. Griffiths, P. R. Eds.; John Wiley and Sons: Chichester.
18 Qian, X.-M.; Nie, S. M. Chem. Soc. Rev. 2008, 37, 912.   DOI   ScienceOn
19 Graham, D.; Goodacre, R. Chem. Soc. Rev. 2008, 37, 883.   DOI   ScienceOn
20 Kneipp, K.; Kneipp, H.; Itzkan, I.; Dasari, R. R.; Feld, M. S. Chem. Rev. 1999, 99, 2957.   DOI   ScienceOn
21 Chan, W. C.; Nie, S. Science 1998, 281, 2016.   DOI
22 Jaiswal, J. K.; Mattoussi, H.; Mauro, J. M.; Simon, S. M. Nat. Biotechnol. 2003, 21, 47.   DOI   ScienceOn
23 Rowe, C. A.; Scruggs, S. B.; Feldstein, M. J.; Golden, J. P.; Ligler, F. S. Anal. Chem. 1999, 71, 431.
24 Bruchez, M., Jr.; Moronne, M.; Gin, P.; Weiss, P.; Alivisatos, A. P. Science 1998, 281, 2013.   DOI
25 Lee, M.; Lee, S.; Lee, J.; Lim, H.; Seong, G. H.; Lee, E. K.; Chang, S.; Oh, C. H. Choo, J. Biosens. Bioelectron 2011, 26, 2135.   DOI   ScienceOn
26 Banholzer, M. J.; Millstone, J. E.; Qin, L. D.; Mirkin, C. A. Chem. Soc. Rev. 2008, 37, 885   DOI   ScienceOn
27 Frens, G. Nat. Phys. Sci. 1973, 241, 20.   DOI
28 Pang, I.; Kim, H.; Kim, S.; Lee, J. Kor. J. Mater. Res. 2008, 18, 664.   과학기술학회마을   DOI   ScienceOn
29 Kim, S.; Pang, I.; Lee, J. Macromol. Rapid Commun. 2007, 28, 1574.   DOI   ScienceOn
30 Yang, Y.; Shi, J.; Tanaka, T.; Nogami, M. Langmuir 2007, 23, 12042.   DOI   ScienceOn
31 Guo, S.; Wang, Y.; Wang, E. Nanotechnology 2007, 18, 405602.   DOI   ScienceOn
32 Driskell, J. D.; Uhlenkamp, J. M.; Lipert, R. J.; Porter, M. D. Anal. Chem. 2007, 79, 4141.   DOI   ScienceOn
33 Cao, Y. C.; Jin, R.; Mirkin, C. A. Science 2002, 297, 1536.   DOI   ScienceOn
34 Qin, L.; Zou, S.; Xue, C.; Atkinson, A.; Schatz, G. C.; Mirkin, C. A. Proc. Nat. Acad. Sci. 2006, 103, 13300.   DOI   ScienceOn
35 Driskell, J. D.; Kwarta, K. M.; Lipert, R. J.; Porter, M. D.; Neill, J. D.; Ridpath, J. F. Anal. Chem. 2005, 77, 6147.   DOI   ScienceOn
36 Lyandres, O.; Shah, N. C.; Yonzon, C. R.; Walsh, J. T., Jr.; Glucksberg, M. R.; Van Duyne, R. P. Anal. Chem. 2005, 77, 6134.   DOI   ScienceOn
37 Nie, S.; Emory, S. R. Science 1997, 275, 1102.   DOI   ScienceOn
38 Ni, J.; Lipert, R. J.; Dawson, G. B.; Porter, M. D. Anal. Chem. 1999, 71, 4903.   DOI   ScienceOn
39 Combs, Z. A.; Chang, S.; Clark, T.; Singamaneni, S.; Anderson, K. D.; Tsukruk, V. V. Langmuir 2011, 27, 3198.   DOI   ScienceOn
40 Offenhausser, A.; Bocker-Meffert, S.; Decker, T.; Helpenstein, R.; Gasteier, P.; Groll, J.; Moller, M.; Reska, A.; Schafer, S.; Schulte, P.; Vogt-Eisele, A. Soft Matter 2007, 3, 290.   DOI   ScienceOn
41 Xia, Y.; Whitesides, G. M. Angew. Chem. Int. Ed. 1998, 37, 550.   DOI   ScienceOn
42 Terry, L. A.; White, S. F.; Tigwell, L. J. J. Agric. Food Chem. 2005, 53, 1309.   DOI   ScienceOn
43 Kanda, V.; Kariuki, J. K.; Harrison, D. J.; Mcdermott, M. T. Anal. Chem. 2004, 76, 7252.
44 Kneipp, K.; Wang, Y.; Kneipp, H.; Perelman, L. T.; Itzkan, I.; Dasari, R. R.; Feld, M. S. Phys. Rev. Lett. 1997, 78, 1667.   DOI   ScienceOn
45 Chen, L.; Park, Y.; Seo, H.; Hong, W.; Jung, Y. M.; Zhao, B. J. Raman Spectrosc. 2011 in press.