• Title/Summary/Keyword: Molecular genetics

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Application of Decision Tree for the Classification of Antimicrobial Peptide

  • Lee, Su Yeon;Kim, Sunkyu;Kim, Sukwon S.;Cha, Seon Jeong;Kwon, Young Keun;Moon, Byung-Ro;Lee, Byeong Jae
    • Genomics & Informatics
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    • v.2 no.3
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    • pp.121-125
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    • 2004

  • The purpose of this study was to investigate the use of decision tree for the classification of antimicrobial peptides. The classification was based on the activities of known antimicrobial peptides against common microbes including Escherichia coli and Staphylococcus aureus. A feature selection was employed to select an effective subset of features from available attribute sets. Sequential applications of decision tree with 17 nodes with 9 leaves and 13 nodes with 7 leaves provided the classification rates of $76.74\%$ and $74.66\%$ against E. coli and S. aureus, respectively. Angle subtended by positively charged face and the positive charge commonly gave higher accuracies in both E. coli and S. aureusdatasets. In this study, we describe a successful application of decision tree that provides the understanding of the effects of physicochemical characteristics of peptides on bacterial membrane.

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Nucleotide Sequencing and PCR-RFLP of Insulin-like Growth Factor Binding Protein-3 Gene in Riverine Buffalo (Bubalus bubalis)

  • Padma, B.;Kumar, Pushpendra;Choudhary, V.;Dhara, S.K.;Mishra, A.;Bhattacharya, T.K.;Bhushan, B.;Sharma, Arjava
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.7
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    • pp.910-913
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    • 2004

  • Insulin-like growth factor binding protein-3 (IGFBP-3) gene is a structural gene associated with the growth and development of the animals. The present investigation was carried out to unravel nucleotide sequence and polymerase chain reactionrestriction fragment polymorphism (PCR-RFLP) of IGFBP-3 gene in buffalo. Genomic DNA was isolated from a total of 157 animals belonging to Murrah, Surti, Jaffarabadi and Nagpuri breeds of Indian riverine buffalo. A 655 bp of IGFBP-3 gene was amplified in all the breeds and amplicons were digested with Hae III, Taq I and Msp I restriction enzymes. On digestion with Hae III yielded single restriction pattern of 8 fragments of sizes 201, 165, 154, 56, 36, 19, 16 and 8 bp in all the animals studied. Similarly Taq I and Msp I also revealed single restriction pattern yielding fragments of sizes 240 and 415 bp and 145 and 510 bp, respectively. This shows nonpolymorphic nature of restriction sites in buffalo. Nucleotide sequencing of 587 bp of IGFBP-3 gene in Murrah buffalo was done and submitted to the GenBank (Accession No. AY304829). Nucleotide sequencing revealed an addition of 4 bases in the intronic region as compared to cattle.

  • https://doi.org/10.5713/ajas.2004.910 인용 PDF KSCI

Genetics and Molecular Biology in Aquaculture - Review -

  • Lakra, W.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.6
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    • pp.894-898
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    • 2001

  • Genetics has played a pivotal role in increasing the world food production through revolutions in plant and animal sciences. Though the attention on fisheries has been inadequate but the growing importance of modern genetic manipulations and biotechnological innovations to aquaculture has been realized. Recent advances in fish genetics and molecular biology have provided a suite of useful techniques, which have several applications in aquaculture. This paper reviews the advancement in the applications of selection, hybridization, chromosome engineering, sex control, gene transfer and molecular technologies for enhanced aquaculture productivity.

  • https://doi.org/10.5713/ajas.2001.894 인용 PDF

Morphological and molecular analysis of indigenous Myanmar mango (Mangifera indica L.) landraces around Kyaukse district

  • Kyaing, May Sandar;Soe, April Nwet Yee;Myint, Moe Moe;Htway, Honey Thet Paing;Yi, Khin Pyone;Phyo, Seinn Sandar May;Hlaing, Nwe Nwe Soe
    • Journal of Plant Biotechnology
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    • v.46 no.2
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    • pp.61-70
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    • 2019

  • There is vast genetic diversity of Myanmar Mangoes. This study mainly focused on indigenous thirteen different mango landraces cultivated in central area of Myanmar, Kyauk-se District and their fruit characteristics by 18 descriptors together with genetic relationship among them by 12 SSR markers. Based on the morpho-physical characters, a wide variation among accessions was found. Genetic characterization of thirteen mango genotypes resulted in the detection of 302 scorable polymorphic bands with an average of 4.33 alleles per locus and an average polymorphism information content (PIC) of 0.7. All the genotypes were grouped into two major clusters by UPGMA cluster analysis and a genetic similarity was observed in a range of 61 ~ 85%. This study may somehow contribute insights into the identification of regional mango diversity in Myanmar and would be useful for future mango breeding program.

  • https://doi.org/10.5010/JPB.2019.46.2.061 인용 PDF KSCI