• Title/Summary/Keyword: Molecular evolution

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Crystal structure and functional analysis of the surE protein identify a novel phosphatase family

  • Lee, Jae-Young;Kwak, Jae-Eun;Suh, Se-Won
    • Proceedings of the Korean Biophysical Society Conference
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    • 2001.06a
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    • pp.19-19
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    • 2001
  • The genome sequencing has revealed a large number of proteins of unknown or little characterized functions that have been well conserved during evolution. It remains a great challenge to decipher the molecular and physiological functions of these proteins. One example of the evolutionarily conserved protein family with little understood function is the surE family.(omitted)

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Effects of Recombination on the Pathogenicity and Evolution of Pepper mottle virus

  • Jonson, Miranda Gilda;Seo, Jang-Kyun;Cho, Hong-Soo;Kim, Jeong-Soo;Kim, Kook-Hyung
    • The Plant Pathology Journal
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    • v.25 no.4
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    • pp.417-421
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    • 2009
  • The analysis of the full length genome of Korean isolates of Pepper mottle virus (PepMoV) in previous study showed molecular variations and are found to be related to symptom variation and pathogenicity (Kim et al., 2009, Virus Res. 144:83-88). To fully understand the molecular variation of PepMoV in Korea, we further assessed the role of RNA recombination to biological variation and evolution of PepMoV. Full-length genome of a total of 17 Korean-PepMoV and 2 American (CA and FL) isolates were examined for possible detection of genetic recombination using different recombination detections programs and detected 5 and 8 tentative recombination events using RDP3 and Splits Tree4 programs, respectively. Interestingly, tentative recombinants detected such as isolates 57, 134 and 217 were previously identified as severe isolates and 205135 and 205136 as differentiating isolates (Kim et al., 2009, Virus Res. 144:83-88). In addition, recombination was frequently detected in the Vb isolate, the first PepMoV isolate reported in Korea, suggesting significant involvement in the evolution of PepMoV in Korea. These initial results of our recombination analyses among PepMoV isolates in Korea may serve as clues to further investigate the biological variations and evolution of PepMoV brought about by recombination.

Evolution of Molecular Hydrogen from Glucose by Rhodopseudomonas sp. KCTC 1437 (Rhodopseudomonas sp. KCTC 1437에 의한 포도당으로부터의 수소 생성)

  • Woo, Seung-Jin;Lee, Jeong-Kug;Kwon, Tae-Jong;Kho, Yung-Hee
    • Microbiology and Biotechnology Letters
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    • v.13 no.3
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    • pp.257-263
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    • 1985
  • Rhodopseudomonas sp. KCTC 1437 evolved molecular hydrogen efficiently under light illuminated anaerobic culture condition in the presence of organic acids and various sugars. especially glucose when low concentration of NH$_4$ + or L-glutamate was added to cultures. It was revealed that hydrogen formation from Rhodopseudomonas sp. KCTC 1437 was mediated by two different enzyme systems. Under the nitrogen limiting condition, hydrogen evolution from glucose was catalyzed by nitrogenase. For the nitrogenase activation in vivo, the precultured cells drown on limiting concentration of NH$_4$$^{+}$ as a sole nitrogen source showed more capacity of hydrogen evolution from glucose in the presence of L-glutamate than any other cells .frown on sufficient concentration of NH$_4$$^{+}$, L-glutamate, NH$_4$$^{+}$, or both of L-glutamate and $N_2$. A significant volume of molecular hydrogen was evolved from glucose even in the presence of excess NH$_4$$^{+}$ either in the light or dark anaerobic condition, presumably due to the mediation of hydrogen evolution by fromic hydrogenlyase.enlyase.

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