• 제목/요약/키워드: Molecular characterization

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Purification and Characterization of Lipase from the Anaerobic Lipolytic Bacterium Selenomonas lipolytica

  • Behere, AdiTi S.;Dighe, Abhijit S.;Bhosale, Suresh B.;Ranade, Dilip R.
    • Journal of Microbiology and Biotechnology
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    • 제12권1호
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    • pp.142-144
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    • 2002
  • Two different extracellular lipases were produced by an anaerobic bacterium, Selenomonas lipolytica. A major lipase, lipase I, was isolated, which showed optimum activity at pH 6.0 and at $45^{\circ}C$. It showed a molecular weight of 240 kDa and was a tetramer of a subunit having molecular weight of 60 kDa, which is different from the known bacterial lipases.

Characterization of the Active Site of Ascorbyl Free Radical Reductase Purified from Pleurotus ostreatus

  • Yu, Seong-Woon;Kang, Sa-Ouk
    • 한국생물물리학회:학술대회논문집
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    • 한국생물물리학회 1998년도 학술발표회
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    • pp.25-25
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    • 1998
  • Ascorbyl free radical reductase was purified from the white rot fungus, Pleurotus ostreatus. The enzyme contained FMN as a prosthetic group, which was reduced by NADH and reoxidized by ascorbyl free radical. Reduction of ascorbyl free radical by the enzyme was observed by EPR spectroscopy.(omitted)

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Trends in Polymer Separations: Better and More Information on Polymer Distributions

  • Schoenmakers, Peter
    • 한국고분자학회:학술대회논문집
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    • 한국고분자학회 2006년도 IUPAC International Symposium on Advanced Polymers for Emerging Technologies
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    • pp.113-113
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    • 2006
  • Polymer separations are used to obtain information on molecular distributions (molecular-weight distribution, chemical-composition distribution, functionality-type distribution, etc.). The existing methods, such as size-exclusion chromatography are reliable, but imperfect. New methods and improvements to existing methods are being studied and some of the results will be discussed. In addition, comprehensive two-dimensional liquid chromatography allows the complete characterization of two mutually dependent distributions. LCxSEC chromatograms provide a very good qualitative impression of the distributions, but calibration is not straightforward. Finally, progress in mass spectrometry allows much better information to be obtained.

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Characterization of Dextran Produced by L. mesenteroides ATCC 13146

  • Yoo, Sun-Kyun
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2005년도 생물공학의 동향(XVII)
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    • pp.358-362
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    • 2005
  • High molecular weight dextran (39% alcohol, v/v), less soluble dextran, eluted from this column between T 500 and T 2000, a commercial linear dextran, Soluble dextran (45% alcohol, v/v) eluted at between T 70 and T 150 dextran. The molecular weight average of total dextran (50% alcohol, v/v) was between 150,000 to 500,000. A few oligosaccharides were detected from hydrolyzates of less soluble dextran. The hydrolyzates of soluble dextran were a family of DP 1 to 6 isomaltooligosaccharides. Compounds greater than DP 4 were branched isomaltooligosaacharides.

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Silk Fibroin Microsphere and Its Characterization

  • Yeo Ju-Hong;Lee Gwang-Gil;Lee Yong-U;Gwon Hae-Yong;U Sun-Ok
    • 한국잠사학회:학술대회논문집
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    • 한국잠사학회 2003년도 제46회 춘계 학술연구 발표회
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    • pp.56-56
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    • 2003
  • Using gel filtration chromatography (GFC), pure separation of high molecular silk fibroin was obtained and silk fibroin microsphere particles (SFMP) could be simply made by spray dryer method. Also, some of its physicochemical properties and morphology were investigated. The average molecular weight (Mw) of pure silk fibroin protein dissolved in calcium chloride is about 61, 500 g/mol as measured by gel permeation chromatography(GPC). (omitted)

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미국흰불나방(Hyphantria cunea Drury) 혈림프부터 apolipophorin-III의 순수정제 및 특성 (Purification and Characterization of Apolipophorin-III from Haemolvmph of Fall Webworm Hvphantria cunea Drury)

  • 윤화경;서신자김학열
    • 한국동물학회지
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    • 제37권4호
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    • pp.488-494
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    • 1994
  • Apolipophorin-III (ApoLp-III) was purified from adult haemolynph of Hyphantriu cuneo and their molecular weight and synthetic place were investigated. ApoLp-III purification was performed by KBr-density gradient ultracentrifugation followed by gel permeation chromatographv (Sephadex G-1001 and ion-exchange chromatography (CM-52) and their purity was confirmed on 10% SDS-PAGE. ApoLp-III has the molecular weight of 18 ItDa and is synthesized by fat body.

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Recent advances in the characterization and the treatment methods of effluent organic matter

  • Ray, Schindra Kumar;Truong, Hai Bang;Arshad, Zeshan;Shin, Hyun Sang;Hur, Jin
    • Membrane and Water Treatment
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    • 제11권4호
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    • pp.257-274
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    • 2020
  • There are many previous review articles are available to summarize either the characterization methods of effluent organic matter (EfOM) or the individual control treatment options. However, there has been no attempt made to compare in parallel the physicochemical treatment options that target the removal of EfOM from biological treatments. This review deals with the recent progress on the characterization of EfOM and the novel technologies developed for EfOM treatment. Based on the publications after 2010, the advantages and the limitations of several popularly used analytical tools are discussed for EfOM characterization, which include UV-visible and fluorescence spectroscopy, Fourier transform infrared spectroscopy (FTIR), size exclusion chromatography (SEC), and Fourier transform-ion cyclotron resonance-mass spectrometry (FT-ICR-MS). It is a recent trend to combine an SEC system with various types of detectors, because it can successfully track the chemical/functional composition of EfOM, which varies across a continuum of different molecular sizes. FT-ICR-MS is the most powerful tool to detect EfOM at molecular levels. However, it is noted that this method has rarely been utilized to understand the changes of EfOM in pre-treatment or post-treatment systems. Although membrane filtration is still the preferred method to treat EfOM before its discharge due to its high separation selectivity, the minimum requirements for additional chemicals, the ease of scaling up, and the continuous operation, recent advances in ion exchange and advanced oxidation processes are greatly noteworthy. Recent progress in the non-membrane technologies, which are based on novel materials, are expected to enhance the removal efficiency of EfOM and even make it feasible to selectively remove undesirable fractions/compounds from bulk EfOM.

부식산의 흡광도, COD, TOC, 분자크기분포 및 오존처리에 따른 특성변화 (Characterization of Humic Acid and its Variation after Ozonation)

  • 이동석;김명철
    • 산업기술연구
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    • 제17권
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    • pp.297-303
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    • 1997
  • This study investigates the characteristics of humic acid before and after ozonation. The results were comapred in such terms as CODCr, CODMn, TOC(total organic carbon) and molecular size distribution. As a result of ozonation (with the dose of 3mgO3/mgHumic Acid), UV NIS absorbance, CODCr, CODMn and TOC were decreased with similar tendency. The mole fraction of high molecular size was rapidly decreased and the fraction ratio of low molecular size was increased through ozone treatment. This fact can be correlated with the change in the difference and the ratio of CODCr and CODMn (the value of CODCr-CODMn and CODMn/CODCr). From this result, it can be inferred that the molecular size and the degree of chemical oxidative degradation of humic acid after ozonation were changed.

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Synthesis and Characterization of Low Molecular Weight Poly(maleic anhydride-co-vinyl acetate) Copolymers

  • Yoon, Kee-Jong;Woo, Jong-Hyung;Seo, Young-Sam
    • 한국섬유공학회:학술대회논문집
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    • 한국섬유공학회 2003년도 The Korea-Japan Joint Symposium
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    • pp.63-64
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    • 2003
  • As a part of a research on the development of polymeric textile finishing agents, polymerization of low molecular weight copolymers containing maleic anhydride residues have been studied. In order to obtain low molecular weight poly(maleic anhydride-co-vinyl acetate) copolymers, the feed ratio of the two monomers and the concentrations of initiator and chain transfer agent were varied in the copolymerization. The copolymers were characterized using GPC, NMR, FTIR, DSC, and TGA. Copolymers with molecular weights in the range 2,150 to 6,630 have been prepared and characterized. The hydrolysis of the anhydride groups of the copolymer in water is also discussed.

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