• Title/Summary/Keyword: Molecular Systematics

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Characterization of Heterochlorella luteoviridis (Trebouxiaceae, Trebouxiophyceae) isolated from the Port of Jeongja in Ulsan, Korea

  • Kim, Kyeong Mi;Kang, Nam Seon;Jang, Hyeong Seok;Park, Joon Sang;Jeon, Byung Hee;Hong, Ji Won
    • Journal of Marine Bioscience and Biotechnology
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    • v.9 no.2
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    • pp.22-29
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    • 2017
  • A unicellular green alga was axenically isolated from the Port of Jeongja, Ulsan, Korea. Morphological, molecular, and biochemical analyses revealed that the isolate belonged to Heterochlorella luteoviridis. This is the first report of this species in Korea. The microalgal strain was named as H. luteoviridis MM0014 and its growth, lipid composition, and biomass properties were investigated. The strain thrived over a wide range of temperatures ($5-30^{\circ}C$) and withstood up to 0.5 M NaCl. The results of gas chromatography/mass spectrometry analysis showed that the isolate was rich in nutritionally important polyunsaturated fatty acids. Its major fatty acids were linoleic acid (35.6%) and ${\alpha}$-linolenic acid (16.2%). Thus, this indigenous marine microalga is a potential alternative source of ${\omega}3$ and ${\omega}6$ polyunsaturated fatty acids, which are currently obtained from fish and plant oils. Ultimate analysis indicated that the gross calorific value was $19.7MJ\;kg^{-1}$. In addition, the biomass may serve as an excellent animal feed because of its high protein content (51.5%). Therefore, H. luteoviridis MM0014 shows promise for applications in the production of microalgae-based biochemicals and biomass feedstock.

Redescription of Two Holostichid Species of Genus Holosticha Wrzesniowski 1877 (Ciliophora, Hypotrichida, Holostichidae) from Seoul, Korea (한국산 전열하무충 속 하모류 2종(유모 문, 하모 목, 전열하모 과)의 재기재)

  • Shin, Mann-Kyoon;Kim, Won
    • Animal Systematics, Evolution and Diversity
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    • v.9 no.2
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    • pp.251-259
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    • 1993
  • The soil hypotrichs collected from the moss-covered soils in the campus of Seoul National University were identified as Holosticha multistylata Kahl, 1928 and H. sylvatica Foissner, 1982. These two species are discovered for the first time from Korea and redescribed with illustrations. The description was based on the observation of living specimens, protargol impregnated specimens and biometric analysis. They are distinguished from congeneric species by their following characteristics : 4 frontal cirri in H. multistylata and 7-8 in H. sylvatica, 3 buccal cirri in H. multistylata and 1 in H. sylvatica, 3 dorsal kineties in H. multistylata and 5 in H. sylvatica.

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New Record of a Sea Urchin Echinometra mathaei (Echinoidea: Camarodonta: Echinometridae) from Jeju Island, Korea and Its Molecular Analysis

  • Lee, Taek-Jun;Shin, Sook
    • Animal Systematics, Evolution and Diversity
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    • v.28 no.3
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    • pp.178-184
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    • 2012
  • Echinoids were collected at depths of 5-10 m in Munseom, Jeju Island by SCUBA diving on November 23, 2008 and September 15, 2009. Two specimens were identified as Echinometra mathaei (Blainville, 1825) based on morphological characteristics and molecular analyses of mitochondrial cytochrome c oxidase subunit I partial sequences. Echinometra mathaei collected from Korea was redescribed with photographs and was compared with other species from GenBank based on molecular data. Phylogenetic analyses showed that no significant differences were between base sequences of E. mathaei from Korea and that from GenBank. To date, 13 echinoids including this species have been reported from Jeju Island, and 32 echinoids have been recorded in Korea.

Ants of Chindo Island in Korea (Hymenoptera, Formicidae)

  • Kim, Byung-Jin;Kim, Ki-Gyong;Ryu, Dong-Pyo;Kim, Joong-Hyon
    • Animal Systematics, Evolution and Diversity
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    • v.11 no.1
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    • pp.101-113
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    • 1995
  • The ants found in Chindo Island were investigated from July to October, 1994. We were interested in the ant fauna of this island because it is one of larger islands of Korea and being well supplied with subterranean water, quite different for mother islands in this respect. Collections were examined from 11 sites of Chindo Is. , of which 21 species of 15 genera under 4 subfamilies are classified and reported.

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Recent Advances of the Diversity, Evolution, and Systematics of White Blister Rusts (Albuginales; Oomycetes) (흰녹가루병균(Albuginales; Oomycetes)의 다양성, 진화, 계통분류학 연구의 진보)

  • Choi, Young-Joon
    • The Korean Journal of Mycology
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    • v.44 no.2
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    • pp.73-81
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    • 2016
  • The Albuginales (Oomycetes) consist of obligate biotrophic pathogens, none of which is culturable on artificial media. This group causes white blister rust disease in diverse angiosperm plants, including many economically important crops such as sunflower, horseradish, rape, radish, spinach, and wasabi. Recent advances in molecular phylogenetic tools and findings of new morphological characters have advanced our knowledge on their diversity, evolution, and systematics. This review introduces the white blister rusts and discusses recent innovations resulting from studies on Albuginales.

Molecular Systematics of Tephritidae (Insecta : Diptera): Testing Phylogenetic Position of Korean Acidiella spp. (Trypetini) Using Mitochondrial 16S rDNA Sequences

  • Han, Ho-Yeon;Ro, Kyung-Eui
    • Animal cells and systems
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    • v.6 no.1
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    • pp.13-18
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    • 2002
  • Phylogenetic relationships of Korean Acidiella species were tested using mitochondrial 16S ribosomal RNA gene sequences. We used 16 published sequences as outgroup, and 10 new sequences for nine Korean Acidiella species as ingroup. The number of aligned sites was 1,281 bp, but 1,135 bp were used for the analysis after excluding sites with missing data or gaps. Among these 1,135 sites, 464 sites were variable and 340 were informative for parsimony analysis. Phylogenetic information was extracted from this data set using neighbor-joining, maximum likelihood and maximum parsimony methods and compared to a morphology-based phylogenetic hypothesis. Our molecular data suggest that: (1) the tribe Trypetini appears to be monophyletic even when the nine additional Acidiella species are added to our previous phylogenetic analysis; (2) all the Korean Acidiella species belong to the Trypeta group, but the genus Acidiella is not supported as monophyletic; (3) the close relationship of A. circumvaga, A. issikii, and A. sapporensis is supported; (4) the close relationship of A. pachypogon and two additional new Acidiella species is strongly supported; and (5) the possible presence of two or more cryptic species among the specimens previously identified as A. obscuripennis is suggested. Sequence data from the mitochondrial 16S rDNA allowed us to better understand the systematic status of Korean Acidiella species. They indicated that the current concept about the genus Acidiella is insufficient and needs to be refined further. This study also showed a few interesting relationships, that had not been recognized by morphological study alone. Based on this study, we were able to plan further experiments to analyze relationships within the Trypeta Group.

Pteriomorphia (Mollusca : Bivalvia) from Ullng Island , Korea (울릉도 해안 익형아강(연체동물문: 이매패강))

  • Byung Lae Choe;Won Kim;Jong Rak Lee;Sook Hee Yoon
    • Animal Systematics, Evolution and Diversity
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    • v.10 no.1
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    • pp.61-83
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    • 1994
  • Thirty-three species or subspecies of marine peteriomorphs from Ullng Island, Korea are described . All the described species are new to the fauna of this island. Among them, five speices, Modiolus (Modiolus) comptus Sowerby, 1915, Gregareiella coralliophaga(Gmelin, 1791) , Musculus (Musculus) laevigatus (Gray, 1824), Chlamys(Coralichlamys) jousseaumei Bavay, 19.4, Spndylus (Spondylus) varius Sowerby, 1829 are newly recorded in Korean waters. These five species are redescribed.

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New Recods of Hypotrichs from Korea (Protozoa, Ciliphora, Hypotrichida) (한국산 미기록 하모충류의 재기재)

  • Shin, Mann-Kyoon;Kim, Hoon-Soo;Kim, Won
    • Animal Systematics, Evolution and Diversity
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    • no.nspc3
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    • pp.15-22
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    • 1992
  • Some hypotrichous ciliates collected from the Han River and the Mountain Kwank were cultured at laboratory. They were identified as Paruroleptus lepisma Wenzel, 1953 and Euplotes aediculatus Pierson, 1943. These two species are reported for the first from Korea. Morphological and biometrical studies of them were carried out by observing both wild and cultured cells and the infraciliature of silver stained specimens. The two speices were redesribed and analyzed biometrically for their taxonomic characters.

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