• Molecules and Cells
• /
• 제21권3호
• /
• pp.315-329
• /
• 2006

Two, well characterized cationic channels, the ryanodine receptor (RyR) and the canonical transient receptor potential cation channel (TRPC) are briefly reviewed with a particular attention on recent developments related to the interplay between the two channel families.

• Journal of Ginseng Research
• /
• 제47권5호
• /
• pp.615-621
• /
• 2023

Korean Red Ginseng (KRG) plays a key role in heme oxygenase (HO)-1 induction under physical and moderate oxidative stress conditions. The transient and mild induction of HO-1 is beneficial for cell protection, mitochondrial function, regeneration, and intercellular communication. However, chronic HO-1 overexpression is detrimental in severely injured regions. Thus, in a chronic pathological state, diminishing HO-1-mediated ferroptosis is beneficial for a therapeutic approach. The molecular mechanisms by which KRG protects various cell types in the central nervous system have not yet been established, especially in terms of HO-1-mediated mitochondrial functions. Therefore, in this review, we discuss the multiple roles of KRG in the regulation of astrocytic HO-1 under pathophysiological conditions. More specifically, we discuss the role of the KRG-mediated astrocytic HO-1 pathway in regulating mitochondrial functions in acute and chronic neurodegenerative diseases as well as physiological conditions.

• Bulletin of the Korean Chemical Society
• /
• 제29권8호
• /
• pp.1505-1509
• /
• 2008

Comparative molecular simulations were performed to establish molecular interaction and inhibitory effect of flavonoid myricetin on formation of amyloid fibris. For computational comparison, the conformational stability of myricetin with amyloid $\beta$ -peptide (A$\beta$ ) and $\beta$ -amyloid fibrils (fA$\beta$) were traced with multiple molecular dynamics simulations (MD) using the CHARMM program from Monte Carlo docked structures. Simulations showed that the inhibition by myricetin involves binding of the flavonoid to fA$\beta$ rather than A$\beta$ . Even in MD simulations over 5 ns at 300 K, myricetin/fA$\beta$ complex remained stable in compact conformation for multiple trajectories. In contrast, myricetin/A$\beta$ complex mostly turned into the dissociated conformation during the MD simulations at 300 K. These multiple MD simulations provide a theoretical basis for the higher inhibitory effect of myricetin on fibrillogenesis of fA$\beta$ relative to A$\beta$ . Significant binding between myricetin and fA$\beta$ observed from the computational simulations clearly reflects the previous experimental results in which only fA$\beta$ had bound to the myricetin molecules.

• 한국인터넷방송통신학회논문지
• /
• 제10권1호
• /
• pp.7-13
• /
• 2010

질소 분위기하에서 분자선 에피탁시 장치로 성장한 GaN 에피층을 고온 열처리 하였다. 시료는 적절한 조건하에서 급속 열처리 후 구조적인 특성의 향상을 나타내었다. 시료의 결정성의 향상은 에피층의 격자 관련 요소들의 흐트러짐의 감소에 기인한다. 에피층의 열처리는 950도의 급속 열처리로를 이용하여 수행하였다. 고온 급속 열처리가 GaN 에피층의 구조적인 특성들에 미치는 효과는 x선 회절을 통하여 연구하였다. x선 회절 스펙트럼에 있어서 Bragg 피크는 열처리 시간이 증가할수록 각도가 큰 쪽으로 이동하였다. 또한 피크의 FWHM은 열처리 시간이 증가함에 따라 약간의 증가 후 감소하였으며 이후 다시 증가하였다. 이와 같은 결과는 급속 고온 열처리된 GaN 에피층에서 격자 상수에 영향을 미치는 인자들이 에피층의 품질을 좋게 하는 방향으로 일률적으로 변화하는 것이 아니라 에피 품질을 나쁘게 하는 방향으로도 변화한다는 것을 의미한다. 적절한 조건 하에서의 급속 열처리는 에피층의 격자 상수에 관여하는 인자들의 흐트러짐을 감소시켜 에피 결정의 질을 향상시킨다.

• 한국정보전자통신기술학회논문지
• /
• 제13권5호
• /
• pp.397-402
• /
• 2020

타입 1 콜라겐 단백질은 인체 내에서 가장 많이 존재하는 단백질이다. 이 단백질은 점탄성 거동을 보이며 이는 힘줄에서도 찾아볼 수 있다. 분자동역학 시뮬레이션 방법에는 rescaling 방법과 reassignment 방법으로 온도를 조절할 수 있다. rescaling 방법은 온도를 주어진 온도로 책정하는 방법이고, reassignment 방법은 원하는 온도로 맥스웰 분포를 이용하여서 온도를 책정하는 방법이다. 우리는 reassignment 방법에서 콜라겐 단백질의 거동이 시간에 따라서 변화하는 현상을 찾아내었다. 반면에 rescaling 방법에서는 시간에 무관하게 거동하였다. 콜라겐에 다른 속도로 인장을 가하였을 경우, 예를 들어 0.5, 1, 2, 5 Å/ps의 속도로 40 Å까지 힘을 가했을 경우, rescaling 방법에서는 속도에 따른 변화가 거의 없었던 반면, reassignment 방법의 경우 대략 80nm, 100nm, 130nm, 180nm까지 인장이 되었음을 보여준다. 이 현상에 대한 물리학적 의미를 명확하게 규명하지는 못하였지만, 단백질에 관한 시뮬레이션을 실행하는데 있어서 주의를 기울여 수행하여야 한다는 점에서 이 논문의 가치가 있다고 생각한다.

• 반도체디스플레이기술학회지
• /
• 제20권1호
• /
• pp.70-76
• /
• 2021

A cylindrical microlens (CML) has been widely used as an optical element for organic light-emitting diodes (OLEDs), light diffusers, image sensors, 3D imaging, etc. To fabricate high-performance optoelectronic devices, the CML with high aspect ratio is demanded. In this work, we report on facile solution-based processes (i.e., slot-die and needle coatings) to fabricate the CML using poly(methyl methacrylate) (PMMA). It is found that compared with needle coating, slot-die coating provides the CML with lower aspect ratio due to the wide spread of solution along the hydrophilic head lip. Although needle coating provides the CML with high aspect ratio, it requires a high precision needle array module. To demonstrate that the aspect ratio of CML can be enhanced using slot-die coating, we have varied the molecular weight of PMMA. We can achieve the CML with higher aspect ratio using PMMA with lower molecular weight at a fixed viscosity because of the higher concentration of PMMA solute in the solution. We have also shown that the aspect ratio of CML can be further boosted by coating it repeatedly. With this scheme, we have fabricated the CML with the width of 252 ㎛ and the thickness of 5.95 ㎛ (aspect ratio=0.024). To visualize its light diffusion property, we have irradiated a laser beam to the CML and observed that the laser beam spreads widely in the vertical direction of the CML.

• 한국정보과학회:학술대회논문집
• /
• 한국정보과학회 2005년도 한국컴퓨터종합학술대회 논문집 Vol.32 No.1 (B)
• /
• pp.259-261
• /
• 2005

수천개의 Gene Expression Measurement를 생성해 내는 DNA Microarray 연구는 조직과 세포의 표본으로부터 진단에 유용한 Gene Expression 정보를 모으게 된다. 이런 종류의 Data를 분석하기 위하여 SVM(Support Vector Machine)을 사용한 새로운 방법이 연구되어왔다. 본 논문에서는 Gene Expression Data에 대한 고유벡터(Eigen Vector)를 이용하여 SVM의 성능을 향상시키고 질병진단에 유용한 Gene을 찾아 내는 알고리즘을 기술한다. 고유벡터를 통하여 Gene을 선택적으로 SVM Learning에 참가 시키고 분류의 결과를 통하여 추가된 Gene이 질병 진단에 미치는 영향력을 알아냄으로써 질병에 대한 Gene 역할을 파악 하는데 활용할 수 있다.

• Bulletin of the Korean Chemical Society
• /
• 제33권5호
• /
• pp.1707-1714
• /
• 2012

Grb2 is an adapter protein involved in the signal transduction and cell communication. The Grb2 is responsible for initiation of kinase signaling by Ras activation which leads to the modification in transcription. Ligand based pharmacophore approach was applied to built the suitable pharmacophore model for Grb2. The best pharmacophore model was selected based on the statistical values and then validated by Fischer's randomization method and test set. Hypo1 was selected as a best pharmacophore model based on its statistical values like high cost difference (182.22), lowest RMSD (1.273), and total cost (80.68). It contains four chemical features, one hydrogen bond acceptor (HBA), two hydrophobic (HY), and one ring aromatic (RA). Fischer's randomization results also shows that Hypo1 have a 95% significant level. The correlation coefficient of test set was 0.97 which was close to the training set value (0.94). Thus Hypo1 was used for virtual screening to find the potent inhibitors from various chemical databases. The screened compounds were filtered by Lipinski's rule of five, ADMET and subjected to molecular docking studies. Totally, 11 compounds were selected as a best potent leads from docking studies based on the consensus scoring function and critical interactions with the amino acids in Grb2 active site.

• Rapid Communication in Photoscience
• /
• 제1권1호
• /
• pp.13-15
• /
• 2012

New porous $TiO_2$ nanostructures with shapes of pearl and rice were synthesized by hydrothermal treatment of $TiO_2$-liposome nanocomposites in acid and base solutions, respectively, as identified by scanning electron microscopy (SEM), transmission electron microscopy (TEM) images and large Brunauer-Emmett-Teller (BET) surface areas. The x-ray diffraction (XRD) patterns and selected area electron diffraction proved them to be well-defined anatase crystals. Their UV-visible reflectance absorption spectra were observed to have low band gap energy (3.03 and 3.07 eV, respectively), exhibiting surface absorption band in the visible range from 400 to 600 nm. The degradation of methylene blue (MB) over the $TiO_2$ nanostructures was observed upon visible-light irradiation, which was found to be very efficient as compared with any other conventional visible-light responsive $TiO_2$ nanostructures.

• Molecules and Cells
• /
• 제41권3호
• /
• pp.168-178
• /
• 2018

Intracellular communication via ubiquitin (Ub) signaling impacts all aspects of cell biology and regulates pathways critical to human development and viability; therefore aberrations or defects in Ub signaling can contribute to the pathogenesis of human diseases. Ubiquitination consists of the addition of Ub to a substrate protein via coordinated action of E1-activating, E2-conjugating and E3-ligating enzymes. Approximately 40 E2s have been identified in humans, and most are thought to be involved in Ub transfer; although little information is available regarding the majority of them, emerging evidence has highlighted their importance to human health and disease. In this review, we focus on recent insights into the pathogenetic roles of E2s (particularly the ubiquitin-conjugating enzyme E2O [UBE2O]) in debilitating diseases and cancer, and discuss the tantalizing prospect that E2s may someday serve as potential therapeutic targets for human diseases.