• Journal of Wetlands Research
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• v.18 no.1
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• pp.58-67
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• 2016

This study was performed to investigate the long-term changes in water quality and benthic ecology around the Nakdong River Estuary. The effect of the estuarine barrage on the ecosystem was also evaluated. The water quality was interpreted using the field survey (2013 and 2014) and monitoring data (MOE, 1989~2013) and the macrobenthic-fauna was investigated through analysis of the field survey data (2014) and literatures review (1985~2013). The long-term variation of water quality of Nakdong River generally showed increased nutrient concentration with decreased discharge, while abrupt influence of the barrage construction was not observed. However, the nutrient and organic matter concentration inside the barrage distinctly was higher than the concentration outside the barrage because the mixing of fresh and seawater was limited by the barrage. Especially, in the period of low discharge during winter, the Chlorophyll-a concentration clearly increased more in the downstream inside the barrage, showing the barrage effect. In other words, stagnant effect caused by barrage construction had an effect on the water quality degradation in dry seasons. As for the benthic ecology inside barrage after barrage construction, molluscans and brackish-water crustaceans disappeared. Outside the barrage, benthic ecosystem has deteriorated and the small-sized organic indicative species like Prionospio membranacea, Pseudopolydora kempi, Sinocorophium sinensis became dominant due to several construction such as Myeong-Gi Bridge, Airport construction, industrial complex after the Nakdong barrage construction.

• Journal of Korean Society of Transportation
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• v.24 no.7 s.93
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• pp.101-114
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• 2006

Recently due to the increase of cars and city life, the traffic congestion has worsened. It Is particularly worse in the center of the metropolis. Within the general public means, the public transport buses have the advantage of being more cheap, accessible and mobile. But as there is no separate lane for buses, the collision of cars and buses are creating damage to public service. In order to solve this situation, the bus priority signal system has been introduced to reduce the bus travel time and improve its services. The purpose of this study is to establish bus priority signal algorithm which builds bus efficiency under the real-time traffic signal control system and to analyze the effect of it. As the green time was calculated against real time (under the real-time traffic signal control system), compared to existing bus priority signal there was a reduction in cross street loss. The modified cycle was used to maintain signal progression. A case study was carried out using VISSIM simulation model. In result of this study, we found that there was a decrease in bus travel time despite some evidence of car delays and compared to existing bus priority signal the delay of dishonor could be reduced dramatically. The analysed result of person delay using MOE, is that there is evidence that when bus priority signal is in effect, the person delay is reduced.

• Journal of Korean Society of Transportation
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• v.30 no.3
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• pp.83-91
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• 2012

The objective of this study is to build a methodology for evaluating intelligent transportation systems (ITS) projects, by selecting measures of effectiveness (MOEs) and developing an approach to collect and process traffic data. While reviewing the existing MOEs and evaluation methodologies for ITS projects, several problems were found, such as the complication in delineating study areas, the absence of standardized evaluation methodologies, and the duplication in selecting MOEs. To tackle these problems, two MOEs capable of directly evaluating traffic conditions were chosen: i.e., average vehicle speed and traffic volume. Both MOEs can not only include all the functions of the existing MOEs, but also be simpler and more objective in evaluating real traffic conditions. The traffic volume can be measured by using either "cordon line" or "all point average" methods. On the other hand, measuring the average vehicle speed depends on site-specific characteristics such as traffic flow states (interrupted or uninterrupted) and traffic conditions (congested or uncongested). The present methodology is easily understandable for anyone and applicable for any ITS project, and is also expected to contribute to building a standardized evaluation system.

• Journal of the Korea Organic Resources Recycling Association
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• v.9 no.2
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• pp.77-86
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• 2001

As construction and expansion of wastewater treatment works is continuing, generation of sludge is increasing. Bur most sludge is not used appropriately but disposed by landfill dumping to sea. Furthermore direct landfilling of sludge cake containing moisture content over 75% has been legally prohibited since 2001 that's enforcement will be more strict in 2003. Such a situation means nowadays recycling of organic waste such as sludge and food waste is necessary. Composting is one of recycling methods commonly and used as an effective means of stabilizing organic waste and then compost can be used as fertilizer. However fertilizer law management which include standard of compost products and other fertilizers applied all sludge products indiscriminiately and was not flexible. So MOE has graded organic composts according to land applications to improve recycle of organic wastes. The classified organic compost which contains low contaminants has been possible to use as various purpose. This study enalyzed 30 samples which were raw materials for compost and compost products management well and to estimate the quality of compost products. Heavy metals were measured in Raw materials and OM/N, NaCl and VS were tested in compost products as well as heavy metals. As a result, approximate 10% of raw materials was not suitable to the grade A and 6.7% over the grade B of the regulation on raw materials for compost. In the case of 30 produced compost propducts approximate, 57% of composts was not compatible with the grade A and B of the regulation on composting product. The qualities of compost products were worse than raw materials, because the compost products have more regulation item raw materials have.

• Journal of The Korean Association For Science Education
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• v.36 no.2
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• pp.325-335
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• 2016

Software (SW) education is guided by the government to operate not only computer subject matter but also related subject matter. SW education is highlighted in the 2015 Revised Curriculum and Guide for Operating SW Education. SW education is related with science education. For example, education on algorithms employing SW and activities using sensors/output control can be an effective strategy for scientific inquiry. The method can also be applied in developing Computational Thinking (CT) in students. In this study, we designed lessons to solve everyday scientific problems using Educational Programming Language (EPL) SW and physical computing materials and applied them to high school students. We conducted surveys that were modified from questionnaires of Internet application capability and based on the standard of accomplishment of SW education as well as elements of CT to find out the change in perceptions on programming and CT of students. We also conducted a survey on students' attitude toward science learning after an SW inquiry activity. In the results, perceptions on programming and CT of students were improved through lessons using unplugged activity, EPL SW, and physical computing. In addition, scores for interest, self-directed learning ability, and task commitment were high.

• Journal of Korean Society of Forest Science
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• v.102 no.3
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• pp.446-454
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• 2013

The present study investigated the biological agents and environmental conditions of the habitats of the Kirengeshoma koreana, a class 2 endangered species class according to the South Korean Ministry of Environment (MOE), elucidated the structural characteristics of the forest stands in the habitats with the importance value and the diversity index, conducted comparative analysis of the factors, and explored their relationship with environmental factors. According to the results of direction analysis of natural habitats of the Kirengeshoma koreana, the direction was distributed to the north or the northeast, and the slope amounted to $15-40^{\circ}$, thus demonstrating that the slope has little effect. In addition, the results showed that the distribution of Kirengeshoma koreana populations dramatically increased starting in areas at least 600 m above the sea level and that the relative light intensity was 3.79% on an average. Most populations were native to gorges and crevices between rocks so that they mainly tended to inhabit areas that could see soil loss in times of precipitation. In addition, a considerable number of the populations were close to hiking trails as well, thus leading to concerns about their artificial destruction. The soil of layer a in the main growth space of the Kirengeshoma koreana was analyzed and turned out to be strongly acidic, measuring pH 4.45-4.89 (average: 4.68). All investigation sections were typical natural broad-leaved tree areas, and, judging from the fact that the Kirengeshoma koreana was most likely to appear in colonies of the Mongolian oak tree (Quercus mongolica Fisch. ex Ledeb.), the Mongolian oak tree seems to be the species of tree that has the greatest effect on the inhabitation of the Kirengeshoma koreana.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.10
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• pp.4409-4419
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• 2015

The Bhopal gas tragedy involving methyl isocyanate (MIC) is one of the most horrific industrial accidents in recent decades. We investigated the genotoxic effects of MIC in long-term survivors and their offspring born after the 1984 occurrence. There are a few cytogenetic reports showing genetic damage in the MIC-exposed survivors, but there is no information about the associated cancer risk. The same is true about offspring. For the first time, we here assessed the micronucleus (MN) frequency using cytokinesis-blocked micronucleus (CBMN) assay to predict cancer risk in the MIC-affected population of Bhopal. A total of 92 healthy volunteers (46 MIC-affected and 46 controls) from Bhopal and various regions of India were studied taking gender and age into consideration. Binucleated lymphocytes with micronuclei (BNMN), total number of micronuclei in lymphocytes (MNL), and nuclear division index (NDI) frequencies and their relationship to age, gender and several lifestyle variabilities (smoking, alcohol consumption and tobacco-chewing) were investigated. Our observations showed relatively higher BNMN and MNL (P<0.05) in the MIC-affected than in the controls. Exposed females (EF) exhibited significantly higher BNMN and MNL (P<0.01) than their unexposed counterparts. Similarly, female offspring of the exposed (FOE) also suffered higher BNMN and MNL (P<0.05) than in controls. A significant reduction in NDI (P<0.05) was found only in EF. The affected group of non-smokers and non-alcoholics featured a higher frequency of BNMN and MNL than the control group of non-smokers and non-alcoholics (P<0.01). Similarly, the affected group of tobacco chewers showed significantly higher BNMN and MNL (P<0.001) than the non-chewers. Amongst the affected, smoking and alcohol consumption were not associated with statistically significant differences in BNMN, MNL and NDI. Nevertheless, tobacco-chewing had a preponderant effect with respect to MNL. A reasonable correlation between MNL and lifestyle habits (smoking, alcohol consumption and tobacco-chewing) was observed only in the controls. Our results suggest that EF and FOE are more susceptible to cancer development, as compared to EM and MOE. The genotoxic outcome detected in FOE reflects their parental exposure to MIC. Briefly, the observed cytogenetic damage to the MIC-affected could contribute to cancer risk, especially in the EF and FOE.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.32 no.2D
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• pp.121-127
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• 2012

One of the traffic problems has been caused by the illegal parking because of the lack of parking space. Especially, the illegal occupancy of the road near an intersection makes an serious reduction of road capacity. The current illegal parking and stop-prohibited areas have been designated for less than 5 meters from the edge or the corner of the 4-leg intersection. However, in reality, the cars parked at the place over 5 meters have had a significant impact on the intersection traffic. In addition, many variables such as intersection type, size, and service levels should be considered as factors which influence on the traffic condition at the intersection. It was assumed that through and right-turn traffic rate have a significant impact on the 4-leg intersection with the illegal parking or stop near it. This study tried to verify the impact through the simulation implementation with MOE and LOS. It shows a guideline to designate the parking or stop-prohibited area near the 4-leg intersection under various traffic conditions.

• Journal of Microbiology and Biotechnology
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• v.28 no.10
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• pp.1749-1759
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• 2018

Recombinant (rec) prion protein (PrP) is an extremely useful resource for studying protein misfolding and subsequent protein aggregation events. Here, we report mass production of high-purity rec-polypeptide encoding the C-terminal globular domain of PrP; (90-230) for human and (89-231) for murine PrP. These proteins were expressed as His-tagged fusion proteins in E. coli cultured by a high cell-density aerobic fermentation method. RecPrPs recovered from inclusion bodies were slowly refolded under reducing conditions. Purification was performed by a sequence of metal-affinity, cation-exchange, and reverse-phase chromatography. The current procedure yielded several dozens of milligrams of recPrP per liter with >95% purity. The purified recPrPs predominantly adopted an ${\alpha}$-helix-rich conformation and were functionally sufficient as substrates to measure the seeding activity of human and animal prions. Establishment of a procedure for high-level production of high-purity recPrP supports the advancement of in vitro investigations of PrP including diagnosis for prion diseases.

• Journal of Microbiology and Biotechnology
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• v.30 no.5
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• pp.668-680
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• 2020

Bacillus velezensis is an important plant growth-promoting rhizobacterium with immense potential in agriculture development. In the present study, Bacillus velezensis Lle-9 was isolated from the bulbs of Lilium leucanthum. The isolated strain showed antifungal activities against plant pathogens like Botryosphaeria dothidea, Fusarium oxysporum, Botrytis cinerea and Fusarium fujikuroi. The highest percentage of growth inhibition i.e., 68.56±2.35% was observed against Fusarium oxysporum followed by 63.12 ± 2.83%, 61.67 ± 3.39% and 55.82 ± 2.76% against Botrytis cinerea, Botryosphaeria dothidea, and Fusarium fujikuroi, respectively. The ethyl acetate fraction revealed a number of bioactive compounds and several were identified as antimicrobial agents such as diketopiperazines, cyclo-peptides, linear peptides, latrunculin A, 5α-hydroxy-6-ketocholesterol, (R)-S-lactoylglutathione, triamterene, rubiadin, moxifloxacin, 9-hydroxy-5Z,7E,11Z,14Z-eicosatetraenoic acid, D-erythro-C18-Sphingosine, citrinin, and 2-arachidonoyllysophosphatidylcholine. The presence of these antimicrobial compounds in the bacterial culture might have contributed to the antifungal activities of the isolated B. velezensis Lle-9. The strain showed plant growth-promoting traits such as production of organic acids, ACC deaminase, indole-3-acetic acid (IAA), siderophores, and nitrogen fixation and phosphate solubilization. IAA production was accelerated with application of exogenous tryptophan concentrations in the medium. Further, the lily plants upon inoculation with Lle-9 exhibited improved vegetative growth, more flowering shoots and longer roots than control plants under greenhouse condition. The isolated B. velezensis strain Lle-9 possessed broad-spectrum antifungal activities and multiple plant growth-promoting traits and thus may play an important role in promoting sustainable agriculture. This strain could be developed and applied in field experiments in order to promote plant growth and control disease pathogens.