• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2003년도 추계학술대회 논문요약집
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• pp.20-20
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• 2003

• BMB Reports
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• 제45권3호
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• pp.153-158
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• 2012

Geft is a guanine nucleotide exchange factor, which can specifically activate Rho family of small GTPase by catalyzing the exchange of bound GDP for GTP. Geft is highly expressed in the excitable tissue as heart and skeletal muscle and plays important roles in many cellular processes, such as cell proliferation, migration, and cell fate decision. However, the in vivo role of Geft remains unknown. Here, we generated a Geft conditional knockout mouse by flanking exons 5-17 of Geft with loxP sites. Cre-mediated deletion of the Geft gene in heart using Mef2c-Cre transgenic mice resulted in a dramatic decrease of Geft expression. Geft knockout mice develop normally and exhibit no discernable phenotype, suggesting Geft is dispensable for the development of the second heart field in mouse. The Geft conditional knockout mouse will be a valuable genetic tool for uncovering the in vivo roles of Geft during development and in adult homeostasis.

• 대한교통학회지
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• 제20권1호
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• pp.19-38
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• 2002

일반적으로 교통운영 시스템에 대한 효과를 평가하기 위해서 구간통행 시간, 통행속도, 지정체율 등과 같은 효과척도(MOE: measure of effectiveness)가 사용된다. 그러나, 에너지에 대한 관심이 극히 높은 상황에서는 기존의 교통측면의 효과척도 이외에 에너지소모라는 효과척도가 교통운영시스템의 MOE로서 대안평가에서 점차로 중요한 영향을 발휘할 수 있다. 본 연구는 교통운영방식별 에너지효율성에 대한 평가를 위해 (기존에 주로 사용되던 효과척도로서의 구간평균속도, 구간평균통행시간, 지정체 뿐 아니라), 에너지 소모 또한 대안평가에 사용할 수 있을 것으로 판단하여, 각 대안별 에너지 소모를 상대적으로 측정하여 볼 수 이는 절차를 개발하고 상호 그 결과를 비교하고자 하였다. 이를 위해 메조모형 시뮬레이터인 Integration이 사용되었으며, HOV 차로 운영, 신호 최적화, 차로 확장, ITS 적용에 대한 대안의 평가를 수행하였다. 이중ITS의 적용이 에너지 절감에 가장 큰 효과가 있는 것으로 나타났으며, 차로확장, 신호최적화, HOV 차로 운영 순으로 에너지 절감 효과가 나타났다. 본 연구는 차종에 대한 특성의 고려, 잠재수요에 대한 고려를 하지 않았으며, 분석 기간이 단지 첨두 1시간에 그쳤기 때문에 향후 이러한 현실적 요소에 대한 고려 및 시스템의 확대적용에 있어 보다 정확한 경제성 분석절차 및 방법론적 개선이 요구된다고 하겠다.

• Geomechanics and Engineering
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• 제12권3호
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• pp.361-397
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• 2017

The unloading effect from excavations can cause the deformation of adjacent tunnels, which may seriously influence the operation and safety of those tunnels. However, systematic studies of the deformation characteristics of tunnels located along side excavations are limited, and simplified methods to predict the influence of excavations on tunnels are also rare. In this study, the simulation capability of a finite element method (FEM) considering the small-strain characteristics of soil was verified using a case study. Then, a large number of FEM simulations examining the influence of excavations on adjacent tunnels were conducted. Based on the simulation results, the deformation characteristics of tunnels at different positions and under four deformation modes of the retaining structure were analyzed. The results indicate that the deformation mode of the retaining structure has a significant influence on the deformation of certain tunnels. When the deformation magnitudes of the retaining structures are the same, the influence degree of the excavation on the tunnel increased in this order: from cantilever type to convex type to composite type to kick-in type. In practical projects, the deformation mode of the retaining structure should be optimized according to the tunnel position, and kick-in deformation should be avoided. Furthermore, two methods to predict the influence of excavations on adjacent tunnels are proposed. Design charts, in terms of normalized tunnel deformation contours, can be used to quantitatively estimate the tunnel deformation. The design table of the excavation influence zones can be applied to determine which influence zone the tunnel is located in.

• Journal of the Korean Wood Science and Technology
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• 제25권1호
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• pp.42-49
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• 1997

The purpose of this study was to investigate the relationships among density, moisture content, and modulus of elasticity in which are important characteristics in physical and mechanical properties of woods. In this study, the dynamic MOE was calculated through the combination with resonance frequency of transverse vibration method and density, and the estimated moisture contents were calculated with two different equations (1, 2) in order to compare with experimental moisture contents. The following results from this study were obtained: 1. According to the regression analysis with two different parameters (E and density), the two regression lines appeared to be straight intersecting at 0.6 density. As another factor, moisture contents in wood also influenced on the analysing regression at the below F.S.P. 2. When considering the relationship between moisture contents and E, the tendency of each moisture content and E showed very similar pattern suggesting that moisture contents in addition to density are very important parameter. 3. When together with moisture contents and density as parameters for multiple regression analysis, coefficiences of determinations are dramatically improved. Interestingly, the coefficiences of determinations are further increased when analysing at the below point of F.S.P. and when analysing higher and lower density separately. In summary, more correct estimation of the dynamic MOE of woods can be possible with only transverse vibration and density in wood. Therefore, with this indirect method, the calculation of MOE in all kinds of woods including timber, live tree and wood products can be feasible resulting in accelerating the efficiency of time and labor.

• Journal of Ginseng Research
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• 제44권3호
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• pp.442-452
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• 2020

Backgroud: Sleep deprivation (SD) impairs learning and memory by inhibiting hippocampal functioning at molecular and cellular levels. Abnormal autophagy and apoptosis are closely associated with neurodegeneration in the central nervous system. This study is aimed to explore the alleviative effect and the underlying molecular mechanism of stem-leaf saponins of Panax notoginseng (SLSP) on the abnormal neuronal autophagy and apoptosis in hippocampus of mice with impaired learning and memory induced by SD. Methods: Mouse spatial learning and memory were assessed by Morris water maze test. Neuronal morphological changes were observed by Nissl staining. Autophagosome formation was examined by transmission electron microscopy, immunofluorescent staining, acridine orange staining, and transient transfection of the tf-LC3 plasmid. Apoptotic event was analyzed by flow cytometry after PI/annexin V staining. The expression or activation of autophagy and apoptosis-related proteins were detected by Western blotting assay. Results: SLSP was shown to improve the spatial learning and memory of mice after SD for 48 h, accomanied with restrained excessive autophage and apoptosis, whereas enhanced activation of phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin signaling pathway in hippocampal neurons. Meanwhile, it improved the aberrant autophagy and apoptosis induced by rapamycin and re-activated phosphoinositide 3-kinase/Akt/mammalian target of rapamycin signaling transduction in HT-22 cells, a hippocampal neuronal cell line. Conclusion: SLSP could alleviate cognitive impairment induced by SD, which was achieved probably through suppressing the abnormal autophagy and apoptosis of hippocampal neurons. The findings may contribute to the clinical application of SLSP in the prevention or therapy of neurological disorders associated with SD.

• Journal of the Korean Wood Science and Technology
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• 제43권4호
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• pp.446-455
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• 2015

국내외 기계등급제재목(구조재 및 층재)의 등급기준 및 설계강도 산출방법을 비교 분석하고 국내 제재산업 실정을 고려한 평균 탄성계수(modulus of elasticity, 이하 MOE) 기준방법 적용을 제안하였다. 먼저 올바른 기계등급제재목 기준 정착을 위해 기계등급구조재와 기계등급층재의 공통점과 차이점을 설명하였다. 최소 고정 MOE 기준 등급을 사용하는 국내 기준은 등급구분에는 편리하나 휨강도(modulus of rupture, 이하 MOR) 예측과 자원이용도 측면에서는 효율성이 낮은 것으로 파악되었다. 해외에서 사용되는 평균 MOE 기준 방법은 초기 컴퓨터 기반 작동을 요구하나 MOR-MOE 직선회귀에 근거한 합리적인 MOR 예측과 품질관리 측면에서 효율성이 높은 것으로 분석되었다. 무엇보다도 현 국내 기계등급구조재 등급체계는 수종별 강도 특성을 반영하지 못하고 있다는 것이 가장 큰 문제점으로 분석되었으며 이러한 결과를 기반으로 MOR-MOE 직선회귀분석에 근거한 기계등급제재목 등급기준 및 기준설계값 산출방법 적용을 제안하였다. 이를 통하여 궁극적으로 부가가치가 높은 국산 기계등급구조재 생산 활성화를 이루고, 기계 등급구조재의 층재 전용 가능에 따른 구조용 집성재 가격경쟁력 제고 효과를 얻을 수 있다고 사료되었다.

• Journal of Microbiology and Biotechnology
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• 제26권4호
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• pp.790-798
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• 2016

Chlamydiae, obligate intracellular bacteria, are associated with a variety of human diseases. The chlamydial life cycle undergoes a biphasic development: replicative reticulate bodies (RBs) phase and infectious elementary bodies (EBs) phase. At the end of the chlamydial intracellular life cycle, EBs have to be released to the surrounded cells. Therefore, the interactions between Chlamydiae and cell death pathways could greatly influence the outcomes of Chlamydia infection. However, the underlying molecular mechanisms remain elusive. Here, we investigated host cell death after Chlamydia infection in vitro, in L929 cells, and showed that Chlamydia infection induces cell necrosis, as detected by the propidium iodide (PI)-Annexin V double-staining flow-cytometric assay and Lactate dehydrogenase (LDH) release assay. The production of reactive oxygen species (ROS), an important factor in induction of necrosis, was increased after Chlamydia infection, and inhibition of ROS with specific pharmacological inhibitors, diphenylene iodonium (DPI) or butylated hydroxyanisole (BHA), led to significant suppression of necrosis. Interestingly, live-cell imaging revealed that Chlamydia infection induced lysosome membrane permeabilization (LMP). When an inhibitor upstream of LMP, CA-074-Me, was added to cells, the production of ROS was reduced with concomitant inhibition of necrosis. Taken together, our results indicate that Chlamydia infection elicits the production of ROS, which is dependent on LMP at least partially, followed by induction of host-cell necrosis. To our best knowledge, this is the first live-cell-imaging observation of LMP post Chlamydia infection and report on the link of LMP to ROS to necrosis during Chlamydia infection.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2008년도 학술발표회 논문집
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• pp.194-198
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• 2008

Significant soil erosion and water quality degradation issues are occurring at highland agricultural areas of Kangwon province because of agronomic and topographical specialities of the region. Thus spatial and temporal modeling techniques are often utilized to analyze soil erosion and sediment behaviors at watershed scale. The Soil and Water Assessment Tool (SWAT) model is one of the watershed scale models that have been widely used for these ends in Korea. In most cases, the SWAT users tend to use the readily available input dataset, such as the Ministry of Environment (MOE) land cover data ignoring temporal and spatial changes in land cover. Spatial and temporal resolutions of the MOE land cover data are not good enough to reflect field condition for accurate assesment of soil erosion and sediment behaviors. Especially accelerated soil erosion is occurring from agricultural fields, which is sometimes not possible to identify with low-resolution MOD land cover data. Thus new land cover data is prepared with cadastral map and high spatial resolution images of the Doam-dam watershed. The SWAT model was calibrated and validated with this land cover data. The EI values were 0.79 and 0.85 for streamflow calibration and validation, respectively. The EI were 0.79 and 0.86 for sediment calibration and validation, respectively. These EI values were greater than those with MOE land cover data. With newly prepared land cover dataset for the Doam-dam watershed, the SWAT model better predicts hydrologic and sediment behaviors. The number of HRUs with new land cover data increased by 70.2% compared with that with the MOE land cover, indicating better representation of small-sized agricultural field boundaries. The SWAT estimated annual average sediment yield with the MOE land cover data was 61.8 ton/ha/year for the Doam-dam watershed, while 36.2 ton/ha/year (70.7% difference) of annual sediment yield with new land cover data. Especially the most significant difference in estimated sediment yield was 548.0% for the subwatershed #2 (165.9 ton/ha/year with the MOE land cover data and 25.6 ton/ha/year with new land cover data developed in this study). The results obtained in this study implies that the use of MOE land cover data in SWAT sediment simulation for the Doam-dam watershed could results in 70.7% differences in overall sediment estimation and incorrect identification of sediment hot spot areas (such as subwatershed #2) for effective sediment management. Therefore it is recommended that one needs to carefully validate land cover for the study watershed for accurate hydrologic and sediment simulation with the SWAT model.

• 한국음향학회:학술대회논문집
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• 한국음향학회 1998년도 학술발표대회 논문집 제17권 2호
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• pp.232-235
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• 1998

In this paper, we present the modified blind adaptive multiuser detector based on Constant Modulus Algorithm(CMA) for the demodulation of code-division multiple-access(CDMA) signals. Convergence issues are treated, and the performance of three algorithms is compared via computer simulations.