• Asian-Australasian Journal of Animal Sciences
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• v.10 no.5
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• pp.505-509
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• 1997

Data were from four institutional herds and four field data collection centers involved in a progeny testing program for Nili-Ravi buffaloes in Pakistan. The REML with a single trait animal model, employed on 2,353 lactations, from 901 daughters of 66 sires, gave a heritability estimate of 0.18 for milk yield with repeatability (between lactations) of 0.43. Estimated milk yield was highest at 65 months of age for the first parity and 81 months for later parities. Correction factors for age at calving, standardized to 60 months in the second and later parities, were developed.

• Clinics in Shoulder and Elbow
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• v.18 no.1
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• pp.52-58
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• 2015

Animal shoulder models are important in the investigation of the natural history of various pathologic conditions and for evaluation of the effectiveness of different treatments and biomaterials. The characteristics of animal shoulder models that may be used for rotator cuff related experiments are different with regard to the anatomy, behavioral pattern, advantages and disadvantages in application to each other. The lower primates and all the non-primate species, except the tree kangaroo, were neither functional overhead nor had a true rotator cuff. Utilizing more advanced primates, or perhaps even the tree kangaroo would be ideal and the most relevant to man as they possess a true rotator cuff. However, ethical concerns, costs, and many limitations in obtaining primates generally preclude the use of these animals for such research. Finally we should consider the differences in comparative anatomy and behavioral pattern of each animal model during performance or interpretation of rotator cuff experiments.

• Biomedical Science Letters
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• v.29 no.4
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• pp.296-301
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• 2023

This study was conducted to induce muscle loss using dexametasone and then use the extract to determine its effectiveness in a muscle loss animal model. Animal experimental groups were divided by five groups. Changes in the weight of the animals were measured for a total of 5 weeks. After animal sacrifice, muscle mass was measured, and animal behavior evaluation was conducted using grip strength test and pole test. The expression levels of MAFbx protein was measured using muscle samples. Oral administration of Fomes fomentarius extract was effective in suppressing muscle atropy and increasing muscle, which was confirmed through animal behavior evaluation and muscle-related protein expression.

• Journal of The Korean Society of Grassland and Forage Science
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• v.36 no.3
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• pp.205-214
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• 2016

The objective of this study was to construct a forage rye (FR) dry matter yield (DMY) estimation model based on climate data by locations in South Korea. The data set (n = 549) during 29 years were used. Six optimal climatic variables were selected through stepwise multiple regression analysis with DMY as the response variable. Subsequently, via general linear model, the final model including the six climatic variables and cultivated locations as dummy variables was constructed as follows: DMY = 104.166SGD + 1.454AAT + 147.863MTJ + 59.183PAT150 - 4.693SRF + 45.106SRD - 5230.001 + Location, where SGD was spring growing days, AAT was autumnal accumulated temperature, MTJ was mean temperature in January, PAT150 was period to accumulated temperature 150, SRF was spring rainfall, and SRD was spring rainfall days. The model constructed in this research could explain 24.4 % of the variations in DMY of FR. The homoscedasticity and the assumption that the mean of the residuals were equal to zero was satisfied. The goodness-of-fit of the model was proper based on most scatters of the predicted DMY values fell within the 95% confidence interval.

• Journal of Animal Reproduction and Biotechnology
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• v.39 no.1
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• pp.2-11
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• 2024

Background: The small intestine plays a crucial role in animals in maintaining homeostasis as well as a series of physiological events such as nutrient uptake and immune function to improve productivity. Research on intestinal organoids has recently garnered interest, aiming to study various functions of the intestinal epithelium as a potential alternative to an in vivo system. These technologies have created new possibilities and opportunities for substituting animals for testing with an in vitro model. Methods: Here, we report the establishment and characterisation of intestinal organoids derived from jejunum tissues of adult pigs. Intestinal crypts, including intestinal stem cells from the jejunum tissue of adult pigs (10 months old), were sequentially isolated and cultivated over several passages without losing their proliferation and differentiation using the scaffold-based and three-dimensional method, which indicated the recapitulating capacity. Results: Porcine jejunum-derived intestinal organoids showed the specific expression of several genes related to intestinal stem cells and the epithelium. Furthermore, they showed high permeability when exposed to FITC-dextran 4 kDa, representing a barrier function similar to that of in vivo tissues. Collectively, these results demonstrate the efficient cultivation and characteristics of porcine jejunum-derived intestinal organoids. Conclusions: In this study, using a 3D culture system, we successfully established porcine jejunum-derived intestinal organoids. They show potential for various applications, such as for nutrient absorption as an in vitro model of the intestinal epithelium fused with organ-on-a-chip technology to improve productivity in animal biotechnology in future studies.

• Archives of Plastic Surgery
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• v.44 no.5
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• pp.361-369
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• 2017

As the popularity of fat grafting research increases, animal models are being used as the source of pre-clinical experimental information for discovery and to enhance techniques. To date, animal models used in this research have not been compared to provide a standardized model. We analyzed publications from 1968-2015 to compare published accounts of animal models in fat grafting research. Data collected included: species used, graft characteristics (donor tissue, recipient area, amount injected, injection technique), time of sacrifice and quantification methods. Mice were most commonly used (56% of studies), with the "athymic nude" strain utilized most frequently (44%). Autologous fat was the most common source of grafted tissue (52%). Subcutaneous dorsum was the most common recipient site (51%). On average, $0.80{\pm}0.60mL$ of fat was grafted. A single bolus technique was used in 57% of studies. Fat volume assessment was typically completed at the end of the study, occurring at less than 1 week to one year. Graft volume was quantified by weight (63%), usually in conjunction with another analysis. The results demonstrate the current heterogeneity of animal models in this research. We propose that the research community reach a consensus to allow better comparison of techniques and results. One example is the model used in our laboratory and others; this model is described in detail. Eventually, larger animal models may better translate to the human condition but, given increased financial costs and animal facility capability, should be explored when data obtained from small animal studies is exhausted or inconclusive.

• Animal Bioscience
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• v.36 no.12
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• pp.1785-1795
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• 2023

Objective: This study was to estimate heritabilities, additive genetic correlations, and phenotypic correlations between number of piglets born alive (NBA), litter birth weight (LTBW), number of piglets weaned (NPW) and litter weaning weight (LTWW) in different parities of Landrace (L), Yorkshire (Y), Landrace×Yorkshire (LY), and Yorkshire×Landrace (YL) sows in a commercial swine operation in Northern Thailand. Methods: Two models were utilized, a single trait repeatability model (RM) and a multiple trait animal model (MTM). The RM assumed reproductive records from different parities to be repeated values of the same trait, whereas the MTM assumed these records to be different traits. The two models accounted for the fixed effects of farrowing year-season, genetic group of the sow, heterosis, and age at first farrowing, and the random effects of sow, boar, and residual. Results: Heritability estimates from RM were 0.02±0.01 for NBA, 0.10±0.01 for LTBW, 0.04±0.01 for NPW, and 0.11±0.01 for LTWW. Heritability estimates from MTM fluctuated across parities, ranging from 0.04±0.01 in parity 2 to 0.09±0.02 in parity 4 for NBA, 0.07±0.02 in parity 2 to 0.16±0.02 in parity 3 for LTBW, 0.04±0.02 in parity 4 to 0.08±0.01 in parity 1 for NPW, and 0.16±0.02 in parity 1 to 0.20±0.02 in parity 2 for LTWW. Additive genetic correlation estimates from MTM were also variable, ranging from 0.29±0.24 between NBA in parity 1 and NBA in parity 2 to 0.99±0.05 between LTWW in parity 3 and LTWW in parity 4. Conclusion: The findings of this study highlight the advantage of using MTM for the genetic improvement of reproductive traits in swine and contribute to the development of sustainable swine breeding programs in Thailand.

• Asian-Australasian Journal of Animal Sciences
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• v.11 no.1
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• pp.84-88
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• 1998

The aim of this study was to describe growth patterns of mice using Gompertz model. Two distinct types of mice, laboratory mouse $CF_{\sharp1}$ (Mus musculus domesticus) and Yonakuni wild mouse (Yk, Mus musculus molossinus yonakuni) were used. From all possible crosses, there were two parental types and two reciprocal $F_1$ crosses obtained. Individual body weights were measured weekly from birth to ten weeks of age on 321 mice. Standardization to six mice was conducted and only first litters were used. Growth curve parameters were estimated to fit growth data. The results showed that growth among genetic groups were significantly different (p < 0.05) for both sexes, in which parental type of $CF_{\sharp1}$ and Yk had the highest and the smallest values, respectively. Meanwhile, reciprocal $F_1$ crosses were intermediate between parental types. It was concluded that Gompertz model provided and excellent fit for the growth data with a high coefficient determination $(R^2 = 0.999)$.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.10
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• pp.1335-1340
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• 2011

Different genetic parameters for weaning weight and wool traits were estimated using restricted maximum likelihood (REML) in Angora rabbits. Total wool yield of first (I), second (II) and third (III) clips were taken as a separate trait under study. The records from more than 2,700 animals were analysed through fitting six animal models with various combinations of direct and maternal effects. A log likelihood ratio test was used to select the most appropriate model for each trait. Direct heritability estimates for the wool traits were found to be moderate to high across different models. Heritability estimates obtained from the best model were 0.24, 0.22, 0.20 and 0.21 for weaning weight, clip I, II and III; respectively. Maternal effects especially due to permanent environment had higher importance at clip I and found to be declining in subsequent clips. The estimates of repeatability of doe effect on wool traits were 0.44, 0.26 and 0.18 for clip I, II and III; respectively. Weaning weight had moderately high genetic correlations with clip I (0.57) and II (0.45), but very low (0.11) with clip III. Results indicated that genetic improvement for wool yield in Angora rabbit is possible through direct selection. Further, weaning weight could be considered as desirable trait for earliest indirect selection for wool yield in view of its high genetic correlation with wool traits.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.7
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• pp.914-918
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• 2004

The random regression model methodology was applied into the estimation of genetic parameters for body weights in Chinese Simmental cattle to replace the traditional multiple trait models. The variance components were estimated using Gibbs sampling procedure on Bayesion theory. The data were extracted for Chinese Simmental cattle born during 1980 to 2000 from 6 national breeding farms, where records from 3 months to 36 months were only used in this study. A 3 orders Legendre polynomial was defined as the submodel to describe the general law of that body weight changing with months of age in population. The heritabilities of body weights from 3 months to 36 months varied between 0.31 and 0.48, where the heritabilities from 3 months to 12 months slightly decreased with months of age but ones from 13 months to 36 months increased with months of age. Specially, the heritabilities at eighteenth and twenty-fourth month of age were 0.33 and 0.36, respectively, which were slightly greater than 0.30 and 0.31 from multiple trait models. In addition, the genetic and phenotypic correlations between body weights at different month ages were also obtained using regression model.