• Microbiology and Biotechnology Letters
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• v.37 no.3
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• pp.273-279
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• 2009

Hyungbangjihwang-Tang (HT), an Oriental herbal formula, has been known to play a role which helps to recover vigor of human in the Orient. In this study, antifungal substance (HTI) was purified from the ethyl-acetate extracts of HT by using $SiO_2$ column chromatography and HPLC, and the antifungal effects of HTI and its mode of action were investigated. By using a broth micro-dilution assay, the activity of HTI was evaluated against fungi. HTI showed antifungal activities without hemolytic effect against human erythrocytes. To confirm antifungal activity of HTI, we examined the accumulation of intracellular trehalose as stress response on toxic agents and effect on dimorphic transition in Candida albicans. The results demonstrated that HTI induced the accumulation of intracellular trehalose and exerted its antifungal effect by disrupting the mycelial forms. To understand its antifungal mode of action, cell cycle analysis was performed with C. albicans, and the results showed HTI arrested the cell cycle at the S phase in yeast. The present study indicates that HTI has considerable antifungal activity, deserving further investigation for clinical applications.

• BMB Reports
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• v.31 no.2
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• pp.149-155
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• 1998

The N199H variant of the EcoRI endonuclease has about twice the catalytic activity of the wild-type. A comparison of their biochemical characteristics, using synthetic oligonucleotides 5'-dAAAACTTAAGAAAAAAAAAAA-3' (KA) and 5'-dTTTTTGAATTCTTTTTTTTTT-3' (KT), helps to define the cleavage reaction pathway of these enzymes. Both EcoRI and EcoRI variant N199H were found to cleave singlestranded KA or KT about three times faster than the double-stranded forms, although the KT oligonucleotide was more susceptible. Using the ssDNA substrate in kinetic analyses, lower $K_m$ values were obtained for the N199H variant than for the wild-type at low (50 mM), as well as high (200 mM), sodium chloride concentrations. This difference between the endonucleases is attributed to a grealter accessibility for tbe substrate by the variant, and also a higher affinity for the DNA backbone. It also appears that the relative activities of the two enzymes, particularly at high ionic strength, are proportional to their populations in the monomeric enzyme form. That is, according to gel filtration data, half of the N199H molecules exist as monomers in 200 mM NaCl, whereas those of the wild-type are mainly dimeric. Consequently, the Asp199 residue of the EcoRI endonuclease may be implicated in the protein-protein interaction leading to dimerization, as well as in coupling to DNA substrates. In summary, it is proposed that active monomeric endonuclease molecules, derived from the dimeric enzyme, recognize and form a complex with a single stranded form of the DNA substrate, which then undergoes nucleophilic substitution and cleavage.

• Applied Biological Chemistry
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• v.38 no.1
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• pp.90-94
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• 1995

Post emergence herbicidal activities$(pI_{50})$ of X-substituted phenylvinylsulfone derivatives(S) in-vivo against rice(Oryza sativa L.), Barnyard grass(Echinochloa crus-galli) and Pickerelweed(Monochoria vaginalis Presl) were measured by the pot test under paddy conditions. The (S) showed herbicidal symptom rapidly with lower activity(average $pI_{50}=2.0$) as proherbicide, which was excellent tolerance to rice. The structure activity relationships(SAR) were analyzed using such a physicochemical parameters as hydrophobic$({\pi})$ and molecular orbital(MO) quantity by the multiple regression technique, and discussed with quantum pharmacology. The herbicidal activities were related to the hydrophobic$({\pi})$ effect of X-substituent and orbital(HOMO & LUMO) energy. In case of Pickerelweed, the effect was rationalized by parabolic function of ${\pi}$ constant, where the optimal value of ${\pi}$ was 1.10. An increase in hydrophobicity and negative orbital energy by the electron attracting X-substituent may contribute to the herbicidal activity. Based on results proposed from SAR analysis, the mode of herbicidal action could be assumed.

• Steel and Composite Structures
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• v.42 no.2
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• pp.277-288
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• 2022

A novel flat steel plate-concrete-corrugated steel plate (FS-C-CS) sandwich panel was proposed for resisting impact load. The failure mode, impact force and displacement response of the FS-C-CS panel under impact loading were studied via drop-weight impact tests. The combined global flexure and local indentation deformation mode of the FS-C-CS panel was observed, and three stages of impact process were identified. Moreover, the effects of corrugated plate height and steel plate thickness on the impact responses of the FS-C-CS panels were quantitatively analysed, and the impact resistant performance of the FS-C-CS panel was found to be generally improved on increasing corrugated plate height and thickness in terms of smaller deformation as well as larger impact force and post-peak mean force. The Finite Element (FE) model of the FS-C-CS panel under impact loading was established to predict its dynamic response and further reveal its failure mode and impact energy dissipation mechanism. The numerical results indicated that the concrete core and corrugated steel plate dissipated the majority of impact energy. In addition, employing end plates and high strength bolts as shear connectors could prevent the slip between steel plates and concrete core and assure the full composite action of the FS-C-CS panel.

• Geomechanics and Engineering
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• v.11 no.2
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• pp.253-267
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• 2016

The stability of surrounding rock will be poor when the tunnel is excavated through nearly horizontal stratum. In this paper, the instability mechanism of local nearly horizontal stratum in super-large section and deep buried tunnel is revealed by the analysis of the macro failure and micro fracture. A structural model is proposed to explain the mechanics of surrounding rock collapse under the action of stress redistribution and shed light on the macroscopic analytical approach of the stability of surrounding rock. Then, some highly effective formulas applied in the tunnel engineering are developed according to the theory of mixed-mode micro fracture. And well-documented field case is made to demonstrate the effectiveness and accuracy of the proposed analytical methods of mixed-mode fracture. Meanwhile, in order to make the more accurate judgment about yield failure of rock mass, a series of comprehensive failure criteria are formed. In addition, the relationship between the nonlinear failure criterion and $K_I$ and $K_{II}$ of micro fracture is established to make the surrounding rock failure criterion more comprehensive and accurate. Further, the influence of the parameters related to the tension-shear mixed-mode fracture and compression-shear mixed-mode fracture on the propagation of rock crack is analyzed. Results show that ${\sigma}_3$ changes linearly with the change of ${\sigma}_1$. And the change rate is related to ${\beta}$, angle between the cracks and ${\sigma}_1$. The proposed simple analytical approach is economical and efficient, and suitable for the analysis of local nearly horizontal stratum in super-large section and deep buried tunnel.

• Magazine of the Korean Society of Agricultural Engineers
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• v.39 no.6
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• pp.67-79
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• 1997

The perpose of this study was to suggest the experimental characteristic of the Cherepnov Water Lifter following the drain mode. The Cherepnov Water Lifter(CWL), which is powered by the potential energy of water, can be set to operate automatically when the water m a tank is drained. In this study, a CWL is constructed in the valve drain controlling mode(VCM) and the siphon drain controlling mode(SCM), and a pressure transducer is installed. It was found that, in the VCM, intake flow volume is proportional to both delivery flow volume and drain flow volume. In the SCM, intake flow volume is proportional to drain flow volume, and the average delivery rate is proportional to both efficiency and the water utilization ratio. Also, in the VCM, the water utilization ratio is 35~49%, efficiency is 62~9O%, average delivery rate is 12.8~81.2$cm^3$/s, and the average drain rate is 14.O~91.5c$cm^3$/s. On the contrary in the SCM, the water utilization ratio is 1.7~38%, efficiency is 3~58%, average delivery rate is 3.1 ~69.2$cm^3$/s, and the average drain rate is shown as 114.5~ 183$cm^3$/s. As a result of the water utilization ratio, efficiency, average delivery rate, and average drain rate are compared, the VCM is found to be superior and the more economical mode. However, the VCM requires manpower and electricity to operate the electronic machinery involved, while the SCM requires no manpower or electricity at all. An economic evaluation of these differences will be necessary in the future. Also, in the SCM, studies to improve water utilization ratio and efficiency, to find the optimum height of the siphon for decreasing the average drain rate, and to determine the radius of curvature of throat have to be conducted in advance, since a large flow rate is drained during the priming action of the siphon.

• Journal of the Korea institute for structural maintenance and inspection
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• v.23 no.2
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• pp.99-106
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• 2019

A wall-frame structure resists horizontal load by the interaction between the flexural mode of the shear wall and the shear mode of the frame, which implies that the frame deflects only by reverse bending of the columns and girders, and that the columns are axially rigid. However, as the height of frame increases the shear mode of frame changes to flexural mode, which is due to the extension and shortening of the columns. An approximate hand method for estimating horizontal deflection and member forces in high-rise shear wall-frame structures subjected to horizontal loading is presented. The method is developed from the continuous medium theory for coupled walls and expressed in non-dimensional structural parameters. It accounts for bending deformations in all individual members as well as axial deformations in the columns. The deformations calculated from the presented approximate method and matrix analysis by computer program are compared. The presented approximate method is more accurate for the taller structures.

• Journal of Microbiology and Biotechnology
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• v.27 no.1
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• pp.43-48
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• 2017

In a previous study, we analyzed the transcriptome of Scolopendra subspinipes mutilans using next-generation sequencing technology and identified several antimicrobial peptide candidates. One of the peptides, scolopendrasin V, was selected based on the physicochemical properties of antimicrobial peptides using a bioinformatics strategy. In this study, we assessed the antimicrobial activities of scolopendrasin V using the radial diffusion assay and colony count assay. We also investigated the mode of action of scolopendrasin V using flow cytometry. We found that scolopendrasin V's mechanism of action involved binding to the surface of microorganisms via a specific interaction with lipopolysaccharides, lipoteichoic acid, and peptidoglycans, which are components of the bacterial membrane. These results provide a basis for developing peptide antibiotics.

• Applied Microscopy
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• v.8 no.1
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• pp.1-8
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• 1978

The aim of this research was to discover the action mechanism of various antituberculosis agents (isoniazid, paraaminosalicylic acid and streptomycin) which act on Mycobacteria tuberculosis hominis $H_{37}R_v$ and also to study the relationship of ultrastructural changes and the growth pattern in Mycobacterium tuberculosis hominis. The ultrastructural change was observed with an electron microscope while the growth pattern was studied through in vitro culture. The results are summarized as follows: 1. The ultrastructural changes found in the group treated only with isoniazid were the loss of nuclear materials and the appearence of electron dense granules. 2. In the group treated with paraaminosalicylic acid, thickening of nuclear filaments and meso some arrangement disorders were observed. 3. In the group treated with streptomycin, the ribosome particles appeared indistinct and the cytoplasm was denaturalized. 4. In the group cross treated with all three agents, all the ultrastructural changes mentioned above could be observed in the cell just as they appeared in the single treated groups. 5. In all of the single and in the crossly treated group, there were no significant changes note in the cell wall or cytoplasmic membranes of any of the cells observed. 6. In the cultural data in vitro, through the crossly treated group and single treated group. growth was observed in 3-5 weeks of culture.

• Wind and Structures
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• v.5 no.2_3_4
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• pp.329-336
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• 2002

The along-wind response of a surface-mounted elastic fence under the action of wind was investigated numerically. In the computations, two sets of equations, one for the simulation of the unsteady turbulent flow and the other for the calculation of the dynamic motion of the fence, were solved alternatively. The resulting time-series tip response of the fence as well as the flow fields were analyzed to examine the dynamic behaviors of the two. Results show that the flow is unsteady and is dominated by two frequencies: one relates to the shear layer vortices and the other one is subject to vortex shedding. The resulting unsteady wind load causes the fence to vibrate. The tip deflection of the fence is periodic and is symmetric to an equilibrium position, corresponding to the average load. Although the along-wind aerodynamic effect is not significant, the fluctuating quantities of the tip deflection, velocity and acceleration are enhanced as the fundamental frequency of the fence is near the vortex or shedding frequency of the flow due to the occurrence of resonance. In addition, when the fence is relatively soft, higher mode response can be excited, leading to significant increases of the variations of the tip velocity and acceleration.