• 미생물학회지
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• 제25권4호
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• pp.304-308
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• 1987

The mode of transglycosylation reaction observed during the action of low-molecular-weight 1,4-$\beta$-D-glucan glucanohydrolase (EC 3.2.1.4) purified from Trichoderma koningii ATCC 26113 was investigated using $^{1}H-NMR $spectroscopy. The H-1 proton resonances were analysed. After reaction of the enzyme with cellotriose, the reaction products were separated by high performance liquid chromatography. H-1 resonances of the products were consisted with those of cellobiose, cellotriose and cellotitraose, respectively. Therefore it was proved that all the reaction products formed by the action of the enzyme on cellooligosaccharides, including transglycosylation products, possess only H-NMR -1,4-glycosidic linkage(s).

• 한국미생물·생명공학회지
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• 제23권6호
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• pp.671-677
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• 1995

An extracellular enzyme showing lytic activity on E. coli peptidoglycan had been isolated from Bacillus sp. BL-29. The lytic enzyme was purified to homogeneity by ion-exchange chromatography and gel filtration, with a recovery of 5%. The enzyme was monomeric and had an estimated molecular weight of 31,000 Da. The mode of action of the purified enzyme was also investigated. When the purified lytic enzyme was incubated with cell wall peptidoglycan, N-terminal amino groups were released without the release of reducing groups. The N-terminal amino acid released was identified as dinitrophenylalanine (DNP-alanine) by analysis of terminal amino acid by dinitrophenylation method. This result suggests that the lytic enzyme should be a kind of N-acetylmura-myl-L-alanine amidase.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2009년도 제40회 하계학술대회
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• pp.1144_1145
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• 2009

Tidal power station is using the difference of the ebb and flow and the single action tidal power is dependent on tide amplitude and basin volume. Therefore the inflow of basin in rainy season has also effect on the daily power. Also if operating units are changed then starting head too changed. Therefore the number of units are very important for the optimization model. According to our study the primary point when we make a determination of optimization is starting head and governorl control mode. On this study optimization model for tidal power station is considered all of this conditions.

• 한국잡초학회지
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• 제18권2호
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• pp.161-170
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• 1998

Fenoxaprop-P-ethyl에 대한 bentazon의 길항작용기작을 검정하기 위하여 4엽기의 벼와 피를 대상으로 bentazon 혼합처리에서 나타나는 fenoxaprop-P-ethyl의 흡수, 이행, 대사 및 작용점의 활성 변화를 조사하였다. Fenoxaprop-P-ethyl에 대하여 벼와 피의 지상부에서 생육억제가 크게 나타났다. Fenoxaprop-P-ethyl 단독처리에 있어서 두 초종 모두에서 처리 직후부터 빠른 속도로 약제가 흡수되어, 총 흡수량의 90%이상이 약제처리 6시간 이내에 이루어졌으며, 처리 24시간까지 흡수량의 약 30%가 처리엽의 상, 하위조직으로 이행되었다. Ethyl ester형으로 처리된 약제는 신속히 acid형으로 전환되었으며, 처리 24시간 후부터는 acid형이 conjugate형의 대사물로 전환되었다. 그러나 bentazon에 의한 fenoxaprop-P-ethyl의 흡수, 이행 및 대사활성의 변화는 인정되지 않았다. In vitro ACCase의 활성은 벼와 피 에서 각각 26.5 및 23.2nmol/min/mg protein으로 나타났다. 이 효소 활성의 50% 저해농도에 있어서 fenoxaprop-P-ethyl 단독처리와 bentazon 혼합처리 사이에 유의성은 인정되지 않았다. 그러나 bentazon 처리에 의하여 in vivo ACCase 활성의 감소와 엽록체 thylakoid에서 전자전달의 억제가 나타났다. 따라서 fenoxaprop-P-ethyl에 대한 bentazon의 길항작용기작은 bentazon이 fenoxaprop-P-ethyl 작용점의 활성에 직접적으로 관여하는 것이 아니라, 엽록체 구조 및 활성 저해에 의한 fenoxaprop-P-ethyl의 작용점 감소를 통하여 간접적으로 fenoxaprop-P-ethyl의 활성을 억제하는 것으로 판단된다.

• Wind and Structures
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• 제29권3호
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• pp.163-175
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• 2019

Multispan suspension bridges make a good alternative to single-span ones if the crossed strait or river width exceeds 2-3 km. However, multispan three-tower suspension bridges are found to be very sensitive to the wind load due to the lack of effective longitudinal constraint at their central tower. Moreover, at certain critical wind speed values, the aerostatic instability with sharply deteriorating dynamic characteristics may occur with catastrophic consequences. An attempt of an in-depth study on the aerostatic stability mode and damage mechanism of three-tower suspension bridges is made in this paper based on the assessment of strain energy and dynamic characteristics of three particular three-tower suspension bridges in China under different wind speeds and their further integration into the aerostatic stability analysis. The results obtained on the three bridges under study strongly suggest that their aerostatic instability mode is controlled by the coupled action of the anti-symmetric torsion and vertical bending of the two main-spans' deck, together with the longitudinal bending of the towers, which can be regarded as the first-order torsion vibration mode coupled with the first-order vertical bending vibration mode. The growth rates of the torsional and vertical bending strain energy of the deck after the aerostatic instability are higher than those of the lateral bending. The bending and torsion frequencies decrease rapidly when the wind speed approaches the critical value, while the frequencies of the anti-symmetric vibration modes drop more sharply than those of the symmetric ones. The obtained dependences between the critical wind speed, strain energy, and dynamic characteristics of the bridge components under the aerostatic instability modes are considered instrumental in strength and integrity calculation of three-tower suspension bridges.

• 전자공학회논문지SC
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• 제37권3호
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• pp.72-83
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• 2000

본 논문에서는 리칭모드 제어기와 신경 회로망을 이용하여 3상 유도전동기의 위치제어 시스템을 구현한다. 리칭모드 제어기는 위치오차와 속도오차의 궤적을 슬라이딩 평면으로 들어가도록 하고 신경회로망의 초기학습을 담당한다. 리칭모드 제어기의 구조는 슬라이딩 평면의 스위칭 함수로부터 간단히 구성하였다. 또한, 신경 회로망은 전향경로 신경망으로 구성되며 비선형 매핑능력과 탁월한 학습능력을 이용하여 유도전동기의 등가제어입력을 학습하도록 하였고 신경 회로망의 입력으로는 모터의 기준속도, 기준위치 및 엔코더를 이용하여 측정된 모터의 실제속도와 위치 등을 이용하였고 온라인 상태로 학습되도록 하였다. 이와 같이 복합적으로 구성된 제어기들을 유도전동기의 위치제어 시스템에 적용하였다. 본 논문에서 제안된 알고리즘의 타당성을 보이기 위해 기존의 PI 제어기와 비교평가를 하였으며 시뮬레이션과 실험결과로부터 초기운전 상태에서는 리칭모드 제어기가 주로 제어를 담당하지만 시간이 지남에 따라 신경회로망이 학습되어 신경 회로망이 주 제어기가 됨을 확인하였다. 아울러, 제안된 제어기가 PI 제어기보다 우수하고 특히 부하변동과 같은 외란에 강인함을 알 수 있었으며, 정상상태 오차가 현저히 감소하여 정밀한 제어가 가능함을 확인하였다.

• Journal of Microbiology and Biotechnology
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• 제13권6호
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• pp.998-1000
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• 2003

This study was carried out to elucidate the mode of bacteriostatic property of xanthostatin (XS), a novel depsipeptide antibiotic with an N-acetylglycine side chain and selective antimicrobial activity against Xanthomonas spp. Two biotransformed XSs were isolated by the treatment of XS with the cell lysate of Xanthomonas campestris pv. citri, a solvent partition, preparative TLC, and HPLC. Structure determination of those two biotransformed XSs demonstrated deletion of the N-acetylglycine side chain. Noteworthily, they showed no antimicrobial activity against Xanthomonas spp. This result suggests that the N-acetylglycine side chain plays a critical role in the antimicrobial activity of XS, and that the bacteriostatic property of XS is due to susceptibility of the ester bond between the hexadepsipeptide nucleus and the N-acetylglycine side chain to hydrolytic enzyme(s) produced by Xanthomonas spp.

• Structural Engineering and Mechanics
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• 제16권5호
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• pp.581-595
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• 2003

Since the conventional direct approaches are hard to be applied for damage diagnosis of complex large-scale structures, a two-step approach for diagnosing the joint damage of framed structures is presented in this paper by using artificial neural networks. The first step is to judge the damaged areas of a structure, which is divided into several sub-areas, using probabilistic neural networks with natural Frequencies Shift Ratio inputs. The next step is to diagnose the exact damage locations and extents by using the Radial Basis Function (RBF) neural network with the second Element End Strain Mode of the damaged sub-area input. The results of numerical simulation show that the proposed approach could diagnose the joint damage of framed structures induced by earthquake action effectively and has reliable anti-jamming abilities.

• 한국통신학회논문지
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• 제22권1호
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• pp.21-29
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• 1997

This paper describes implementation contents contents of manual/automatic complex redundancy control mothod for control of a modulation system of a Paging earth station in redundancy stracture. The existed redundancy control method usually is a automatic local control method in which the redundancy switching, including display or alarm beeping through operation of display devices or audio devices, is performed by the co-action of components or modules when the abnormal status is occurred in a modulation system. However, this method introduced in here is designed to enable use of three control modes;1) Manual mode by an operator, 2) Auto-remote mode by the Network Maagement System, through implementation of the redundancy control system composed of the redundancy control board and the redundancy switching circuit.

• Structural Engineering and Mechanics
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• 제27권5호
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• pp.609-623
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• 2007

The problem of a semi-infinite magneto-electro-elastically impermeable mode-III crack in a magneto-electro-elastic material is considered under the action of impact loads. For the case when a pair of concentrated anti-plane shear impacts, electric displacement and magnetic induction impacts are exerted symmetrically on the upper and lower surfaces of the crack, the magneto-electro-elastic field ahead of the crack tip is determined in explicit form. The dynamic intensity factors and dynamic energy density factor are obtained. The method adopted is to reduce the mixed initial-boundary value problem, by using the Laplace and Fourier transforms, into three simultaneous dual integral equations, one of which is converted into an Abel's integral equation and the others into a singular integral equation with Cauchy kernel. Based on the obtained fundamental solutions of point impact loads, the solutions of two kinds of different loading cases are evaluated by integration. For some particular cases, the present results reduce to the previous results.