• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.4
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• pp.1134-1139
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• 2004

This study was conducted to investigate the application possibility of leaf and stem extract(LSE) from the mixture of leaf and stem of ginseng radix(Panax Ginseng CA Meyer). This study measured antifatigue effects by rota-rod test and swimming test. Also we examined the cell toxicity in normal liver and kidney cells, and acute toxicity in mice. Dropping times of LSE treated group decreased about 39-83% compared to the control group according to supplementation intake levels. Swimming time in LSE treated group increased compared to the control group at short and long supplementation. Negative effects were not found from the results of the cell toxicity. Also acute toxicity not shown. These results imply that the leaf and stem of ginseng radix could be used as possible food resources and functional food material and feed stuff.

• Journal of Environmental Science International
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• v.23 no.7
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• pp.1269-1275
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• 2014

Ecotoxicity assessments with the physiochemical water quality items and the bioassay test using Daphnia magna were conducted for 18 selected effluents of 6 industrial types (metal processing, petroleum refining, synthetic textile manufacturing, plating, alcohol beverage manufacturing, inorganic compound manufacturing) being detected toxicity from industrial effluent in Ulsan city, and the interrelationship between total toxic unit (${\Sigma}TU$) and concentrations of Water Quality Conservation Act in Korea were investigated. The average toxic unit(TU) of effluents for 6 industrial types displayed the following ascending order: petroleum refining (0.2) < synthetic textile manufacturing (0.6) < alcohol beverage manufacturing (0.9) < metal processing (1.3) ${\leq}$ inorganic compound manufacturing (1.3) < plating (3.0). These values were less than effluent permission standard. Based on the result of substances causing ecotoxicity, the correlation analysis was not easy because most of heavy metals were not detected or were less than effluent permission standard. Toxicological assessment of industrial effluent was suitable for the evaluation of the mixture toxicity for pollutant. The whole effluent toxicity test using a variety of species was needed for the evaluation of industrial wastewater.

• The Korean Journal of Pesticide Science
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• v.5 no.4
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• pp.57-61
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• 2001

Tank mixing application of pesticides has been used to reduce labor and to control wide spectrum of pests, but it may cause significant pesticide poisoning on human and animals due to carelessness. The toxic response of pesticides for the tank mixtures and mixtures was investigated to determine acute toxicity and enzymatic change using experimental animals. Acute oral toxicity and acute dermal toxicity were tested by RDA test guideline. The $LD_{50}$ was calculated by probit analysis method and cholinesterase was measured with automatic analyzer. The toxicities were generally higher than estimated toxicities in tank mixing and mixture. Serum cholinesterase activity was inhibited more than expected at the dose levels of 1/5, 1/10 and 1/20 of $LD_{50}$. Therefore, the results of this study showed that acute toxicity caused by the pesticide mixtures should be considered before the tank mixing method is applied.

• Korean journal of applied entomology
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• v.43 no.1
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• pp.71-79
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• 2004

Toxicities of 42 pesticides (13 acaricides, 13 insecticides, 13 fungicides and 3 adjuvants) commonly used to control rose insect, mite, and disease pests were evaluated to the two-spotted spider mite, Tetranychus urticae egg and adult, and its predator Phytoseiulus persimilis egg, nymph and adult at the recommended concentration. The effect of density suppression of T urticae by predatory mite and pesticide mixture on the rose in the greenhouses was also investigated. Among 13 acaricides tested, acequinocyl, bifenazate, fenbutatin oxide and spirodiclofen showed much less toxicity to P. persimilis than to T urticae. Among insecticides, acetamiprid, imidacloprid, spinosad, thiamethoxam and acetamiprid＋etofenprox showed low toxicity to P. persimilis. and T ruticae. Among 13 fungicides, azoxystrobin, kresoxim-methyl, myclobutanil, nuarimol, triadimefon, triflumizole and oxadixyl＋mancozeb had a negligible effect on P. persimilis and T. urticae. Among three adjuvants, cover and siloxane expressed high toxicity, while spreader showed very low toxicity to P. Persimilis. In the greenhouses experiments, the density of T urticae before treatment was 65.3 mites per leaf. However, their density after release about 30 predatory mites per rose abruptly decreased from 3.8 mites at 11th day to zero mite at 20th day. During survey periods, four treatments of fungicides (kresoxim-methyl, myclobutanil, nuarimol, triflumizole) for the control of Sphaerotheca pannosa and one treatment of insecticide (spinosad) the control of Frankliniella occidentalis were applied, and these treatments had no the pesticides had no effect on the predatory mite density. It may be suggested from these results that four acaricides, five insecticides, seven fungicides, and one adjuvant could be incorporated into the integrated T. urticae management system with P. persimilis on rose cultivation.

• Journal of Physiology & Pathology in Korean Medicine
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• v.23 no.5
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• pp.1125-1131
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• 2009

Lophatheri Herba, Patriniae Radix, Alli tuberosi Herba, and Menthae Herba have long been used externally for treatment of injuries or inflammations in Korea. This study was carried out to examine the anti-dandruff effect of the mixture made with equal parts of 70%-ethanol extracts of Lophatheri Herba, Patriniae Radix, Allii tuberosi Herba, and Menthae Herba (LPAM) and of each one above, compared with 1%-zinc pyrithione (Standard) used commercially as anti-dandruff. The results showed the mixture was more effective than each crude drug. The 10%-LPAM produced no toxicity in skin reaction test and eye irritation test, and showed significant antimicrobial activity against Pityrosporum ovale, the major cause of dandruff, and also showed significant anti-dandruff and itch-improvement effects on head skin.

• Biomolecules & Therapeutics
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• v.7 no.2
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• pp.185-190
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• 1999

The current study was performed to determine the acute oral toxicity of P55A, a crude extract of 1 : 1 mixture of Phellodendron amurense cortex and Aralia elata cortex, in SD rats and beagle dogs. 5 rats of each sex were treated with a single dose of P55A orally at doses of 0 and 5,000 mg/kg respectively. Also 2 dogs of each sex were treated with a single dose of P55A orally at doses of 0 and 2,000 mgAg, respectively. After the treatment, clinical signs, and body weight change were observed for 14 days. All rats survived during the study and did not show any clinical sign. Body weight gain showed no significant difference between the control and treated rats. Grossly, no lesion was observed in the rats. All dogs survived during the study. In clinical signs, dark stool was observed in the 2,000 mg/kg treated dogs at day 1 after administration. The animals recovered from general signs at day 2 after administration. Body weight gain showed no significant difference between the control and treated dogs. Grossly, no lesion was observed in the dogs. It is suggested that the LD$_{50}$ of P55A by oral administration was estimated to be over 5,000 mg/kg in both sexes of rats and 2,000 mg/kg in both sexes of beagle dogs.s.

• Journal of Korean Society on Water Environment
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• v.31 no.2
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• pp.87-93
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• 2015

In this study, the inhibitory effect of toxic metals was investigated on the activated sludge of the municipal sewage treatment plant. The allowable concentration of toxic metals was also estimated for the stable operation of the biological treatment process. The single and mixture toxicity of $Cu^{2+}$, $Al^{3+}$ and $Zn^{2+}$ were evaluated for the activated sludge microorganisms. As a result, nitrifying microorganisms were more susceptible than heterotrophic microorganisms. $IC_{10}$ (Inhibition Concentration of 10%) of $Cu^{2+}$, $Al^{3+}$ and $Zn^{2+}$ for the nitrifying microorganisms was 3 mg/L, 7 mg/L and 25 mg/L, respectively. The mixture toxicity showed three times more sensitive than the single toxicity. The concentrations of $Cu^{2+}$, $Al^{3+}$ and $Zn^{2+}$ to minimize the inhibitory effect on organic matter removal and nitrification in batch experiments were found to be 1.3 mg/L, 2.5 mg/L and 6.3 mg/L.

• Journal of Food Hygiene and Safety
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• v.29 no.4
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• pp.383-390
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• 2014

The safety of a new natural plant composition (ADP) was assessed on the genotoxicity study and 14-day repeat dose toxicity study. ADP contains a mixed water extract obtained from the mixture of Phellodendron cortex (Phellodendron amurense) and Anemarrhena rhizoma (Anemarrhena asphodeloides), and poses the contractile properties mediated by alpha-adrenoceptor of the prostate and urethra as well as antioxidant and anti-inflammatory properties. In order to evaluate genetic safety, in vivo micronucleus test was performed in ICR mice orally administered with three dose levels of 1250, 2500, 5000 mg/kg body weight, and vehicle and positive control. In the 14 days study, Sprague-Dawley rats were treated with ADP at the dose levels of 500, 1000, 2000 mg/kg once a day, and clinical signs, body weights, hematology, serum biochemistry, necropsy findings and organ weights were monitored and examined. In experimental results, ADP treatment, compared with vehicle control, did not induce the micronucleated erythrocytes from mouse bone marrow. In the 14 days study, any significant and toxicological differences in all measurements of parameters were not observed in ADP treatment groups of animals, compared with vehicle treatment. The No-Observed-Adverse-Effect-Level (NOAEL) of ADP in the 14 days study was determined to be greater than 2000 mg/kg/day in both sexes.

• Korean Journal of Veterinary Research
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• v.44 no.3
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• pp.379-387
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• 2004

When an organism is exposed to various toxicants chronically, reactive oxygen species(ROS) are accumulated and eventually result in several biological effects from gene expression to cell death. In the present study we investigated the oxidative damage of 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin(TCDD) and/or benzo(a)pyrene (B(a)P) in C100 cells. C100 cells treated with TCDD(30 nM) and B(a)P($3{\mu}M$) underwent diverse oxidative stress as determined through thiobarbituric acid-reactive substances(TBARS) formation, DNA fragmentation, DNA single strand break(SSB) assay, immunohistochemical staining of 8-hydroxy-2'-deoxyguanosine(8-OHdG), and mRNA expressions of antioxidant enzymatic genes such as Cu/Zn-SOD gene, GPx(glutathione peroxidase 5) gene, and catalase gene. Lipid peroxidation in C100 cells was determined through measuing the formation of TBARS. For theat, the cells were pretreated with TCDD(30 nM) and/or B(a)P($3{\mu}M$) for 0.5, 1, 2 and 4 days. TBARS formation was increased in TCDD(30 nM) and B(a)P($3{\mu}M$) and mixture($30nM\;TCDD+3{\mu}M\;B(a)P$) and positive control treatment groups comparing to the controls. Mixture treatment induced more DNA fragmentation than the single treatment group at day 6. Also, SSB in all treatment groups was clearly observed when compared with the negative control group. As with the expression of antioxidant enzyme, GPx 5mRNA, B(a)P alone and mixture($30nM\;TCDD+3{\mu}M\;B(a)P$) treatment were higher comparing to those of the negative control and TCDD treatment groups. Our results suggest that exposure of C100 cells to mixture of TCDD and B(a)P leads to significant oxidative damage comparing to the exposures to the individual chemicals. Mechanisms of action are discussed. Additional studies are needed to elucidate the detailed mechanism of mixture-induced toxicity.

• Journal of Korean Society on Water Environment
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• v.33 no.5
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• pp.556-562
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• 2017

Heavy metals in water systems are being managed on the concentration-based guidelines in Korea. However, various chemicals present in water can interact with heavy metals affecting their toxicity. Such interactions are not considered in the concentration-based guidelines. This study investigated the effect of hardness and coexisting heavy metals on heavy metal toxicity to emphasize the importance of having the effect-based guidelines together with the concentration-based guidelines in water management. The toxic effects of Cd, Zn, or mixtures of Cd and Zn were studied with Daphnia magna as a test species following the standard test method at different hardness conditions (100, 200, and $300mg\;L^{-1}$ as $CaCO_3$). The toxicities of single metal solutions and mixtures showed a decreasing trend with increasing hardness, and this can be attributed to the competition between heavy metals and cations such as calcium ions ($Ca^{2+}$) that cause hardness. The predicted toxicities of the heavy metal mixtures from the single metal toxicity deviated from the measured toxicities, and the predicted toxic effects tend to be greater than the measured toxic effects suggesting that Cd and Zn are in competition. This shows the limitations of using predicted toxic effects and the needs for further studies on mixture toxicities. Overall, this study shows that the management of heavy metals in waters needs to employ the effect-based guidelines together with the concentration-based guidelines.