• Journal of Food Hygiene and Safety
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• v.4 no.1
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• pp.37-44
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• 1989

The present work was to study the changes of nonvolatile amines and microorganism in fermentation of anchovy during 12 weeks with addition of various concentration of sodium chloride. Changes of histamine occured significantly during fermentation of anchovy with 10, IS, 20% salt and 10% mixed salts (5.0% NaCl+5.0% KCl). A maximum histamine content was observed in anchovy fermented for 6 weeks while the change of histamine content was not with addition of 20% sodium chloride. Tyramine was found at highest contents in the fermented anchovy of 10% mixed salts and increased markedly in all anchovy fermented for 8 weeks. Cadaverine content was higher in fermented for all fermentation periods than in raw. During fermentation cadaverine contents increased significantly in fermented with 10% mixed salts. In contrast with that, fermented anchovy with 20% sodium chloride had very low those content and high sodium chloride concentration had influenced on amine formation. Although the highest content of putrescine was observed in fermented for 8 weeks, those content was not changed significantly during fermantation. The growth of Microflora, Achromobacter, Aeromonas and Pseudomonas were found in the in itial fermantation and Micrococus, Pediococcus, Lactobacillus and Saccharomyces were found during all fermentation periods.

• The Korean Journal of Food And Nutrition
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• v.24 no.1
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• pp.117-123
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• 2011

This study was conducted to establish process conditions for plant-originated lactic acid production using a mixed culture of plant originated lactic acid bacteria, Lactobacillus sakei B2-16, and Lactobacillus plantarum P23, which were isolated from kimchi, and Bacillus subtilis, which was TP6 isolated from Denjang. Soybean medium was pretreated for 10 minutes at $110^{\circ}C$ and hydrolyzed with 0.2%(w/v) cellulase at $55\sim60^{\circ}C$ for at least 2 hrs. The quality of the final fermentation product was influenced by the inoculation ratio of the Lactobacillus sakei B2-16, Lactobacillus plantarum P23, and Bacillus subtilis TP6. The optimum microorganism inoculation ratio was 1:0.7:0.3, Lactobacillus sakei B2-16: Lactobacillus plantarum P23: Bacillus subtilis TP6, respectively. The sensory characteristics of the product were a refreshing sourness and a soft flavor.

• Journal of Wetlands Research
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• v.19 no.4
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• pp.443-450
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• 2017

The objective of this study was to isolate algae growth inhibiting microorganism to biologically control Microcystis aeruginosa, which is a harmful cyanobacterium. Various bacterial strains were isolated in this study, and four bacterial strains of M1~M4 exhibited remarkable growth inhibiting activity against M. aeruginosa. Based on the 16S rRNA analysis, the isolated M1~M4 strains were identified, and isolated four strains were rod-type and gram-negative. In particular, as well as respective single strain, co-culture of the isolated M1~M4 strains showed obvious algicidal activity against M. aeruginosa. When mixed four strains were inoculated, about 50% of the chlorophyll a was reduced after two days, about 70% after four days, and about 80% after seven days. From these results mentioned above, the four bacterial strains may contribute to the control of harmful M. aeruginosa.

• Korean Journal of Veterinary Service
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• v.13 no.2
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• pp.130-140
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• 1990

A field test using Nophazin (drying off period therapeutics) was done from May, 1989 to October, 1989. 299 quarters of 75 cows from 28 herds were tested. The results were summarized as follows. 1. At the day 7 after parturition, CMT-was 81.60%, clots were removed perfectly, removal rate of hard, hot, pain was 99.6%, only one hard 0.35% was maintained. Recovery rate of general symptoms was 100%. 2. Recovery rate in Nophazin treatment was 100% in Micro-coccus SPP, Corynebacterium SPP, and unisolated, 97.06% in Enterobacterid SPP, 95.58% in Staphylococcus SPP, 91.67% in other Bacilli, 89.19% in Streptococcus SPP, Overall recovery rate was 95.32%, And fungi did not react. 3. The ratio of isolated microorganisms was Staphylococcus SPP 49.8%, Streptococcus SPP 16.3%, Enterobacteria SPP 15.0%, Micrococcus SPP 7.9% Corynebacterium SPP 4.4%, Pseudomonas SPP 2.6%, Bacilli 2.6% and Fungi 1.3%. 4. The range of sensitivity of each microorganism to therapeutics is as follow : Staphylococcus SPP 61.1∼94.7%, Streptococcus SPP 51.4∼91.9%, Micrococcus SPP 44.4∼94.4%, Corynebacterium SPP 70∼80%, Enterobacteria SPP 11.8∼88.2%, and other Gram negative bacilli 8.3∼83.3%. Overall sensitivity was in the range of 50.4∼92.0%, Microorganisms showed the highest sensitivity (80∼94.7%) to Nophazin. 5. New intramammary infection rate was 3.96% (9 strains) at paturition, 8.37% (19 strains) at the day 7 after paturition. Persistent organism wits 6.17% .(14 strains). 6. There was no side effect of Nophazin treatment.

• Journal of the Korea institute for structural maintenance and inspection
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• v.22 no.6
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• pp.24-29
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• 2018

The properties of concrete with addition of microgel - containing hydrogel support were investigated. As a result of measuring the slump of the self - healing concrete, the target slump was satisfied in all the mixing conditions, but the slump was decreased as the mixing amount of the hydrogel support increased. The change of porosity due to incorporation of hydrogel support was minimal. As a result of the evaluation of the compressive strength of the self - healing concrete, the incorporation of the hydrogel support did not affect the strength. However, under the same mixing condition, the dispersion value of the specimens tended to increase with increasing hydrogel support contents. As a result of the permeability test of self-healing concrete according to the incorporation of hydrogel support, it was confirmed that the mixing ratio of hydrogel support was effective to decrease the permeability coefficient.

• Korean Journal of Environmental Agriculture
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• v.38 no.4
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• pp.262-268
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• 2019

BACKGROUND: To investigate mineralization characteristics of organic resources in the soil, five materials (rice straw, cow manure sawdust compost, microorganism compost, mixed oil-cake, and amino acid fertilizer) were treated according to the nitrogen content, and an indoor incubation experiment was conducted for 128 days. The results of this analysis were applied to determine the nitrogen mineralization pattern of these organic resources. METHODS AND RESULTS: During the constant temperature incubation period, the nitrogen net mineralization rate of the organic resources was the highest in the amino acid fertilizer with the highest nitrogen content, and the lowest in the rice straw with the lowest nitrogen content. A positive correlation (0.96) was observed between the potential nitrogen mineralization rate and total nitrogen content. The mineralization rate constant, k, was negatively correlated with the organic matter (-0.96) and carbon content (-0.97). The nitrogen mineralization rate during the first cropping season, as estimated by the model, was 6.6%, 11.6%, 30.9%, 70.7%, and 81.0% for the rice straw, the cow manure sawdust compost, the microorganism compost, the mixed oil-cake, and the amino acid fertilizer, respectively. CONCLUSION: The nitrogen mineralization rate varies depending on the type of organic resources or the nitrogen content; thus, it can be used as an index for determining the nitrogen supply characteristics of the organic resource. Organic resources such as compost with low nitrogen content or those undergoing fermentation contain organic nitrogen. Organic nitrogen is stabilized during the composting process. Therefore, as the nitrogen mineralization rate of these resources is lower than that of non-fermented organic resources, it is desirable to use the fermented organic materials only to improve soil physical properties rather than to supply nutrients for the required amount of fertilizer.

• Korean Journal of Medicinal Crop Science
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• v.8 no.2
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• pp.109-116
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• 2000

This study was carried out to screen herbs among herbal plants showing antimicrobial activity against some food spoilage and pathogenic microorganisms. One hundred fifteen kinds of herbal plants were extracted by 70% ethanol, and then they have been screened for antimicroorganisms. Six herbal plants such as Salviae radix, Dryopteris rhizoma, Terminaliae fructus, Araliae radix, Psoraleae fructus and Schisandrae fructus showed strong antimicrobial activities against Bacillus subtilis. Antimicrobial activities were showed in Anemarrhena radix and Dryopteris rhizoma on Candida albicans, and in Anemarrhenae radix, Dryopteris rhizoma and Polygalae radix on Schizosaccharomyces sp. It was revealed that eight herbal plants such as Dryopteris rhizoma, Salviae radix, Sappan ligunum, Sinomeniae radix, Schisandrae fructus, Rhui fructus, Sophorae radix and Inulae radix also showed antimicrobial activities on Streptococcus mutans. In addition, Anemarrhena radix, Curcuma tuber, Inulae radix, Polygonum radix, Sanguisorbae radix, Scutellariae radix and Terminaliae fructus and showed antimicrobial activities on Trichophyton mentagrophytes. Four kinds of herbal plants such as Dropteris rhizoma, Salviae radix, Terminaliae fructus and Scutellariae radix which showed broad antimicrobial spectrums were mixed by 1 : 1 ratio with the other herbal paints showing relatively strong microbial activities such as Terminaliae fructus, Sinomeniae radix and Scutellariae radix etc. The extracts of mixed herbal paints showed higher antimicrobial activities than those of single herbal plant.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.3
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• pp.450-457
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• 2011

Roasted soybean flour (RSF) and mixed cereals were fermented by the solid state fermentation using Bacillus subtilis HA to optimize the production of biologically active compounds. The RFS fermented with 52.7% moisture content showed higher production with protease activity of 42.6 unit/g and 10% mucilage content after fermentation for 24 hr. Tyrosine content and protease activity after 48 hr fermentation time were the highest values with 445.5 mg% and 55.1 unit/g, respectively. However, the wholesome fermented RSF (FRSF) was obtained by fermentation for 24 hr because of the production of unpleasant flavors after fermentation for 48 hr. The RSF fermented with various types and contents of cereals has no effects on tyrosine content and protease activity. However, the addition of brown rice significantly increased mucilage content, especially indicating 24.55% at the addition of 80% (w/w). For addition of barley, fibrinolytic activity was increased to 11.82 unit/g by the fortification of 60% barley. It is concluded that biologically active compounds including fibrinolytic activity and mucilage content in FRSF were dependent upon the type and content of various cereals.

• Journal of the Korean GEO-environmental Society
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• v.17 no.11
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• pp.35-43
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• 2016

In this study, by using a Effective Microorganisms Brewing Cycle, it confirmed the purification effect of pollutants that are adsorbed on the basins stench removal and retarding soil. On the basis of on-site application test, a soil decontamination system will be suggested. Using a Effective Microorganisms Brewing Cycle, the odor concentration is reduced 2.5 times than that of natural purification treatment method. It was measured and found that the quality of the pore water discharged from the soil is improved. In addition, it was found that a composite of copper and lead with the fermentation microorganisms adsorbed on soil particles from the surface of the stirred experiments lagoon mixed soil is reduced to 65% and 66%, respectively, The TPH organic component was confirmed that the reduction effect of 85%. Restoration of reservoir contaminated soils using the effective microorganism brewing cycle needs to be more developed and implemented as a long-term purification system. This study may be a good reference of developing more complete microorganism brewing system which will efficiently reduce the odor and soil contamination based on optimal stirring and mixing ratio of the compound solutions and contaminated soils in reservoir.

• Journal of the Korea Organic Resources Recycling Association
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• v.11 no.1
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• pp.149-153
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• 2003

This study was conducted to survey the utilization possibility of composting system of small scale static pile with animal manure produced from cattle shed and the effect of addition of microorganism on the maturity of compost. Microorganisms added in composting substrate were bacteria+lactobacillus(BL)+photo.(BLP). The composting practiced was a windrow system without aeration equipment and turning was practiced periodically. The water content of substrate mixed with cow manure, rice husk, and sawdust was about 60%. The temperature during the composting process was increased at over $60^{\circ}C$ within 3 days after composting starting. Increase of temperature at the early stage of composting was fasten in BLP and BL than Control. Because the pH of the raw material was high, the changes of pH during composting was little and stabilized in weak alkaline condition. EC value was high for accumulation of manure and urine excreted continuously by animal and the changes of those during composting occurred in 5~10% increase. Reduction rates of C/N ratio were the largest as the 22.7% in BLP and 19.2and 17.5% in BL and Control respectively. In the evaluation of phytotoxicity, there was stabilized within the short time in BLP and not the difference between BL and Control. Treatment of animal manure produced from small scale cattle shed was possible by using the small scale static pile composting system with reasonable water content and turning and the addition of microorganism in composting substrate was effected on the temperature increase at the early stage of composting and reduction of plant toxicity compounds but little on the maturity of compost.