• Journal of The Korean Society of Grassland and Forage Science
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• v.32 no.4
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• pp.369-378
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• 2012

This study was performed out to investigate the influence of the mixed application of chemical fertilizer (CF) and liquid swine manure (LSM) on the growth characteristics, dry matter yield, amino acids, minerals, and free sugars in cultivating silage corn on paddy soils. The field experiment was designed in a randomized block design of 3 repetitions with CF 100% treatment (C), CF 70% + LSM 30% treatment (T1), CF 50% + LSM 50% treatment (T2), CF 30% + LSM 70% treatment (T3), and LSM 100% treatment (T4). At this time, the application of LSM was based solely on the nitrogen. Ear length, ear circle, stem diameter, and stem hardness of the silage corn did not show significant differences between treatments. Fresh yield, dry matter yield and TDN yield were highest in T3, whereas the lowest in C treatment (p<0.05). Crude protein, crude fat, and crude ash content were significantly higher in T1, C, and T4 treatment, respectively (p<0.05). However, NDF, ADF and crude fiber content did not show significant difference between treatments. The total mineral content decreased significantly (p<0.05) as the LSM application rate increased. Total composition amino acid content was higher in the order of T1 > T2 > C > T4 > T3 treatment (p<0.05). Free sugar content was higher in the order of T1 > T3 > T4 > T2 > C treatment (p<0.05). Based on the above results, suggests that the mixed application of chemical fertilizer 30~50% and LSM 50~70% (T2 and T3) is the most effective, considering the yield performance and the content of sugar degree and free sugar affecting silage.

• Asian-Australasian Journal of Animal Sciences
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• v.11 no.6
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• pp.697-701
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• 1998

In order to improve silage quality and utilization of napier grass (Pennisetum purpureum Schum.) by goats, the grass was ensiled with molasses (MOL) and/or defatted rice bran (DRB). Napier grass was harvested at the growing stage in July and cut into 3 cm length. The grass was mixed with 4% MOL and/or 15% DRB, ensiled 15 kg each into plastic bags and stored for 9 months. Dry matter content of the silage ensiled with MOL (MOL-silage) was 13.4%, but increased to 20% with DRB addition. The addition of MOL decreased pH value and ammonia nitrogen content, but increased lactic acid content. MOL-silage contained about 6% spoilage, but addition of DRB decreased spoilage to less than 1%. Goats were fed the silage at a level of 2.25% (DM basis) of their body weight. Goats fed DRB- or MOL/DRB-silages maintained nitrogen retention, but goats fed MOL-silage did not. The rumen fluid of goats fed DRB-silage tended to be higher in acetic acid and lower in propionic acid than those fed the other silages. Ammonia in the rumen fluids, urea nitrogen in the blood and the urinary nitrogen excretion were the lowest in goats fed MOL/DRB-silage. As the result, the ratio of retained nitrogen to nitrogen intake was the highest in goats fed MOL/DRB-silage. In conclusion, addition of DRB to napier grass increased DM of silage and decreased the volume of spoilage. The combination of MOL and DRB can improve the fermentation quality and thus enhance the utilization of the silage by goats, more than the MOL or DRB being as a single treatment.

• Journal of The Korean Society of Grassland and Forage Science
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• v.41 no.4
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• pp.302-307
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• 2021

In the current study, lactic lactic acid bacteria (LAB) Lactobacillus plantarum and Pediococcus pentosaceus were used as a mixed additive for the production of Orchardgrass silage by ensiled method and nutritional change fermentation ability and microbial content of experimental silages. The addition of LAB to Orchardgrass during ensiling process rapidly reduced the pH of the silages than the non-inoculated silages. In addition, the lactic and acetic acid content of silage was increased by LAB strains than the non-inoculated silages whereas butyric acid content was reduced in silage treated with LAB. A microbiological study revealed that higher LAB but lower yeast counts were observed in inoculated silages compared to non-inoculated silage. Overall data suggested that the addition of LAB stains could have ability to induce the fermentation process and improve the silage quality via increasing lactic acid and decreasing undesirable microbes.

• Animal Bioscience
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• v.36 no.5
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• pp.720-730
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• 2023

Objective: This study investigated the effects of corn silage as a source of microbial inoculant containing antifungal and carboxylesterase-producing bacteria on fermentation, aerobic stability, and nutrient digestibility of fermented total mixed ration (FTMR) with different energy levels. Methods: Corn silage was used as a bacterial source by ensiling for 72 d with an inoculant mixture of Lactobacillus brevis 5M2 and L. buchneri 6M1 at a 1:1 ratio. The corn silage without or with inoculant (CON vs MIX) was mixed with the other ingredients to formulate for low and high energy diets (LOW vs HIGH) for Hanwoo steers. All diets were ensiled into 20 L mini silo (5 kg) for 40 d in quadruplicate. Results: The MIX diets had lower (p<0.05) acid detergent fiber with higher (p<0.05) in vitro digestibilities of dry matter and neutral detergent fiber compared to the CON diets. In terms of fermentation characteristics, the MIX diets had higher (p<0.05) acetate than the CON diets. The MIX diets had extended (p<0.05) lactic acid bacteria growth at 4 to 7 d of aerobic exposure and showed lower (p<0.05) yeast growth at 7 d of aerobic exposure than the CON diets. In terms of rumen fermentation, the MIX diets had higher (p<0.05) total fermentable fraction and total volatile fatty acid, with lower (p<0.05) pH than those of CON diets. The interaction (p = 0.036) between inoculant and diet level was only found in the immediately fermentable fraction, which inoculant was only effective on LOW diets. Conclusion: Application of corn silage with inoculant on FTMR presented an antifungal effect by inhibiting yeast at aerobic exposure and a carboxylesterase effect by improving nutrient digestibility. It also indicated that fermented feedstuffs could be used as microbial source for FTMR. Generally, the interaction between inoculant and diet level had less effect on this FTMR study.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.4
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• pp.523-528
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• 2007

This study was conducted to evaluate the feeding value of jambo grass (Sorghum $bicolour{\times}Sorghum$ sudanefe) silage and mott grass (Pennisetum purpureum) silage as a replacement of conventional fodder (jambo grass) in the diet of lactating Nili buffaloes (Bubalus bubalis). Thirty early-lactating ($45{\pm}4$ days), multi-parous Nili buffaloes, ten in each group, were allotted to three experimental diets. Jambo grass and mott grass were ensiled with molasses (at 2% of fodder DM) in two trench silos for 30 days. The control diet (JG) contained 75% jambo grass while the other two diets contained 75% jambo grass silage (JGS) and 75% mott grass silage (MGS). The remaining 25% DM in each diet was supplied by concentrates. Diets were mixed daily and fed twice a day ad libitum for 120 days. Dry matter intake (DMI) was higher with the JG diet compared with JGS and MGS diets. However, DMI as % body weight did not differ significantly in buffaloes fed either fodder or silage based diets. Crude protein (CP), digestible CP and NDF intakes were significantly higher on JG compared with silage-based diets. Apparent total tract digestibilities of DM, CP and NDF were similar in buffaloes fed JG, JGS and MGS diets. Milk yield (4% FCM) was similar in buffaloes fed JG and silage based diets. Fat, total solids, solid not fat, CP, true protein and non-protein nitrogen content of milk were similar in buffaloes fed fodder or silage based diets. The present results indicated that jambo grass and mott grass ensiled with 2% molasses for 30 days could safely replace the conventional fresh grass fodder (75% DM) in the diet of lactating Nili buffaloes without affecting their milk yield.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.1
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• pp.62-72
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• 2016

This study investigated the dynamics associated with prolonged ensiling and aerobic deterioration of whole crop corn (WCC) silages and total mixed ration (TMR) silages containing WCC (C-TMR silages) to clarify the differences that account for the enhanced aerobic stability of TMR silages. Laboratory-scale barrel silos were randomly opened after 7, 14, 28, and 56 d of ensiling and were subjected to analyses of fermentation quality, microbial and temperature dynamics during aerobic exposure. WCC and C-TMR silages were both well preserved and microorganisms were inhibited with prolonged ensiling, including lactic acid bacteria. Yeast were inhibited to below the detection limit of 500 cfu/g fresh matter within 28 d of ensiling. Aerobic stability of both silages was enhanced with prolonged ensiling, whereas C-TMR silages were more aerobically stable than WCC silages for the same ensiling period. Besides the high moisture content, the weak aerobic stability of WCC silage is likely attributable to the higher lactic acid content and yeast count, which result from the high water-soluble carbohydrates content in WCC. After silo opening, yeast were the first to propagate and the increase in yeast levels is greater than that of other microorganisms in silages before deterioration. Besides, increased levels of aerobic bacteria were also detected before heating of WCC silages. The temperature dynamics also indicated that yeast are closely associated with the onset of the aerobic deterioration of C-TMR silage, whereas for WCC silages, besides yeast, aerobic bacteria also function in the aerobic deterioration. Therefore, the inclusion of WCC might contribute to the survival of yeast during ensiling but not influence the role of yeast in deterioration of C-TMR silages.

• Animal Bioscience
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• v.34 no.9
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• pp.1479-1490
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• 2021

Objective: Our recent findings confirmed the effectiveness of sodium metabisulfite (SMB) in controlling the growth of undesirable microorganisms in fruit and vegetable discards (FVD); however, lactic acid bacteria (LAB) are susceptible to its antibacterial effects. Two series of experiments were conducted to enable the survivability of LAB during silage fermentation of a total mixed ration (TMR) containing SMB-treated FVD. Methods: In Exp. 1, the objective was to isolate a strain of LAB tolerable to the toxic effect of SMB. In Exp. 2, the SMB load was minimized through its partial replacement with a chemical mixture (CM) based on sodium benzoate (57%), potassium sorbate (29%), and sodium nitrite (14%). FVD was treated with SMB + CM (2 g each/kg biomass) and added to the TMR at varying levels (0%, 10%, or 20%), with or without KU18 inoculation. Results: The KU18 was screened as a presumptive LAB strain showing superior tolerance to SMB in broth medium, and was identified at the molecular level using 16S rRNA gene sequence analysis as Lactobacillus plantarum. Inoculation of KU18 in TMR containing SMB was not successful for the LAB development, biomass acidification, and organoleptic properties of the resultant silage. In Exp. 2, based on the effectiveness and economic considerations, an equal proportion of SMB and CM (2 g each/kg FVD) was selected as the optimal loads for the subsequent silage fermentation experiment. Slight differences were determined in LAB development, biomass acidification, and sensorial characteristics among the experimental silages, suggesting the low toxicity of the preservatives on LAB growth. Conclusion: Although KU18 strain was not able to efficiently develop in silage mass containing SMB-treated FVD, the partial substitution of SMB load with the CM effectively alleviated the toxic effect of SMB and allowed LAB development during the fermentation of SMB + CM-treated FVD in TMR.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.4
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• pp.522-528
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• 2018

Objective: This study evaluated inclusion of buckwheat silage to the diet of growing lambs in terms of meat quality as compared to maize silage. Methods: Buckwheat, rich in total phenols (TP, 33 g/kg dry matter [DM]), was harvested at the end of the milk stage and ensiled in 40 kg plastic bags after wilting (294 g/kg silage DM). A total of 18 growing lambs ($21.6{\pm}1.2$) were individually fed isonitrogenous and isoenergetic total mixed rations (TMR) for 75 d that either contained buckwheat or maize silage at DM proportions of 0.50. At the end of feeding trail all lambs were slaughtered to assess carcass characteristics and meat quality. Results: Buckwheat silage increased (p<0.01) the DM intake of lambs as compared to maize silage, but had no effects (p>0.05) on live weight gain and feed efficiency. Carcass weight, dressing percentage, meat pH, water holding capacity, cooking loss, shear force ($kg/cm^2$), and total viable bacteria count of meat did not differ (p>0.05) between the treatments. However, TP content of meat increased (p<0.001) by feeding buckwheat TMR. Feeding buckwheat TMR also decreased (p<0.05) the b* values of meat. Conclusion: The results provide that buckwheat silage is palatable and could successfully include TMR of growing lambs with no adverse effects on performance, carcass and meat quality. Additionally, feeding buckwheat silage to lambs offers increased TP in meat.

• Journal of The Korean Society of Grassland and Forage Science
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• v.30 no.1
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• pp.49-58
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• 2010

This experiment was carried out to estimate the nutritive value of mixed-sowing winter forage crop and whole crop barley by investigating the effects of feeding mixed-sowing winter forage crop and whole crop barley in KBG (Korean black goat) on feed intake, average daily body weight gain, nutrient digestibility and nitrogen retention. The 12 male KBG were divided into four experimental groups - i.e. T1: barley silage added group, T2: mixed-sowing hairy vetch silage added group, T3: mixed-sowing field peas added silage, T4: mixed-sowing Italian rye grass added silage group. Three KBG per each treatment were allotted into individual metabolic cages by Latin-square design. The results from this study are as follow. The DMI (dry matter intake) and organic matter intake in T4 were significantly (p<0.05) higher than those in T1 and T3 and crude protein intake in T4 also recorded the highest among treatments (p<0.05). The highest values in intakes of ADF and NDF were observed in T4 followed by T2, T1 and T3 in order. The average daily body weight gains in T4 and T2 were significantly (p<0.05) higher than those in TI and T3. The dry and organic matter digestibility in T2 and T4 were significantly (p<0.05) higher than those in T1 and T3. The crude protein digestibility in T1 was significantly (p<0.05) lower than those in T2 and T4. The nitrogen intakes in T2 and T4 were significantly (p<0.05) higher than those in T3. The results obtained from this study suggested that the feeds supplemented with whole crop barley with hairy vetch and Italian rye grass mixture silage increased KBG productivity resulted from increases in feed intake, nutrient digestibility and nitrogen retention.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.4
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• pp.516-519
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• 2009

This study was conducted to investigate the effects of replacing whole crop corn silage (WCCS) with whole crop rice silage (WCRS) in the total mixed ration (TMR) on nutrient intake, milk yield and its composition in Holstein cows. The Chucheong rice variety (Oryza sativa L. Japonica) and corn (Pioneer 32 P75) were harvested at yellow-ripe stage and were ensiled in round bales and in trench silos, respectively. Two TMR containing either WCCS or WCRS were prepared. These diets were randomly assigned to 16 midlactating Holstein cows (8 cows per treatment) and were fed for 120 days. The first 20 days were used for dietary adaptation and for the next 100 days daily feed intake, milk yield and its composition were recorded. The pH, lactic acid, NDF, ADF, CP, Ca and P contents were similar in WCRS and WCCS. The DM, ash and EE contents of WCRS were greater compared with WCCS. Nutrient (DM, NDF, TDN and CP) intakes were similar in cows fed WCCS- and WCRS-based TMR. Daily and 4% fat corrected milk yield were not affected by the treatments. Milk composition (percent milk fat, protein, lactose and total solids) was similar in cows fed either WCCS- or WCRSbased TMR. The concentration of milk urea N was greater in cows fed WCRS-based TMR than those fed WCCS-based TMR. In conclusion, round-baled WCRS can replace WCCS in the diet of mid- to late-lactating Holsteins without any deleterious effects on feed consumption, milk yield and its composition. The present findings raise the possibility that WCRS can be used as an alternative roughage source in the diets of dairy cows in countries with surplus rice production.