• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.213.2-214
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• 2003

Three mistletoe lectins (ML -I, ML -IIU, ML -IIL) have been identified in Europe based on sugar specificities for galactose(Gal) and N-acetyl galactosamine(GalNAc). Korean mistletoe lectins have been known as mainly ML -II type. In previous results, we suggested that there are two lectins, 64 KDa and 60 KDa, in Korean mistletoe lectin (KML -C). (omitted)

• 약학회지
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• 제38권4호
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• pp.418-424
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• 1994

Lectin from mistletoe (Viscum coloratum) were obtained by salt fractionation, gel filtration and anion exchange chromatography. A molecular weight of about 60,000D has been determined by SDS-PAGE and two basic subunits which have molecular weights of 33,000D and 28,000D are linked by a disulfide bond. The partially purified mistletoe lectins agglutinated human B erythrocytes. Agglutinating activity was relatively stable at varied pH $(3.77{\sim}8.71)$ and at temperature range of $0{\sim}40^{\circ}C$ and not affected by 9 metal ions. Galactose, lactose and N-acetyl-D- galactosamine inhibited agglutinating activity of lectin. Lectin from mistletoe was more mitogenic to murine lymphocytes than concanavalin A.

• 한국식품영양과학회지
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• 제27권2호
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• pp.359-365
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• 1998

This study was carried out to investigate mutagenecity and antimutagenic effects from crude extract, heating extract and alcohol extract of Korean mistletoe(Viscum album L.) on the bacterial short-term tests, such as Ames test, spore rec-assay, SOS spot test and SOS chromotest by using several kinds of mutagens. In the Ames test, each extract did not show any mutagenesis, but each extract showed inhibitory effects of 80∼95% and 70∼94% against mutagenesis induced by 3-amino-1, 4-dimethyl-5H-pyrido[4,3-b] indole(Trp-P-1) and 2-aminofluorene(2-AF) in Salmonella typhimurium TA98, respectively. In th spore rec-assay, mistletoe ectracts showed antimutagenic effect with inhibiton zone in the range of 5∼11mm against mutagenicity induced by mitomycin C(MMC, 18mm) and N-methyl-N'-nitro-N-nitrosoguanidne (MNNG, 24mm), respectively. The heating and alcohol extracts in the SOS chromotest showed 96% and 70% inhibition against benzo-α-pyrene[B(α)P] and Trp-P-1 induced mutagenesis, respectively.

• 한국당과학회:학술대회논문집
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• 한국당과학회 2006년도 제1회 연례학술대회
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• pp.63-63
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• 2006

• 한국당과학회:학술대회논문집
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• 한국당과학회 2006년도 제1회 연례학술대회
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• pp.64-64
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• 2006

• 한국자원식물학회지
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• 제29권6호
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• pp.650-657
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• 2016

Research has found that the management of the host plant is essential to mistletoe cultivation. A Trunk injection test on the host plant that contained a mixture of indole-3-butryc acid (IBA) and liquid fertilizer was conducted with respect to the improvement of the one year survival rate of mistletoe. As a result, the trunk injection experiments showed the effect of the IBA and liquid fertilizer mixture in all treatments. This mixture was effective to increase the survival rate of mistletoe by 20% with the IBA at 100 mg/L and Hyponex at 100 mg/L. The examination proved that the host plant fertilizer effect was the most effective treatment for organic fertilizer with 60% added NPK (4-2-1). Its effects were higher compared to the control at the length and diameter of one-years-old branches in the host plant, even when the parasitic mistletoe improved its growth in length, diameter, and number of branches. Comparing the control and host plant fertilizer, the latter was the most effective way to process 20 kg per a treatment effect in the experimental site and to process at any time after the inoculation. This treatment is effective to improve the growth of mistletoe by watering the host plant three times per week. Therefore, the management of the host plant is an essential element in the successful cultivation of mistletoe, not only to supply fertilizer and plant hormones to the host plant in the initial inoculation time but also to provide organic fertilizer and irrigation for the host plant.

• BMB Reports
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• 제39권5호
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• pp.560-570
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• 2006

Mistletoe (Viscum album) lectins, which are classified as a type II ribosome-inactivating protein (RIP) due to their unique biological function and the potential medical and therapeutic application in cancer cells, receive a rising attention. The heterodimeric glycoproteins contain the A-chain with catalytic activity and the B-chain with sugar binding properties. In recent years, studies involving the lectins from the white berry European mistletoe (Viscum album) and the yellow berry Korean mistletoe (Viscum album coloratum) have been described. However, the detailed mechanism in exerting unique cytotoxic effect on cancer cells still remains unclear. Here, we aim to understand and define the molecular basis and biological effects of the type II RIPs, through the studies of the recombinant Korean mistletoe lectin. To this end, we expressed, purified the recombinant Korean mistletoe lectin (rKML), and investigated its molecular characteristics in vitro, its cytotoxicity and ability to induce apoptotic cell death in cancer cells. To gain structural basis for its catalytic activity and sugar binding properties, we performed homology modeling studies based on the high degree of sequence identity and conserved secondary structure prediction between Korean and European, Himalayan mistletoe lectins, and Ricin.

• 생약학회지
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• 제40권3호
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• pp.205-212
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• 2009

Antitumor activity of Korean mistletoe extract (KM-110) and European commercial mistletoe preparation (Helixor) was investigated. KM-110 showed the cytotoxic effect that it is high for various tumor cell lines and normal splenocytes in comparison with Helixor. Administration of two mistletoe extracts ($100{\mu}g$) to mice did not show any significant changes on the level of glutamic-oxaloacetic transaminase (GOT), glutamic-pyruvate transaminase (GTP), blood creatinine (CRE) and blood urea nitrogen (BUN) in sera. The culture supernatant of macrophages stimulated with KM-110 inhibited effectively tumor growth whereas Helixor had little effect. Administration of KM-110 or Helixor resulted in a effective inhibition of lung metastasis after the i.v. inoculation of colon 26-M3.1 lung carcinoma, B16-BL6 melanoma and L5178Y-ML25 lymphomas. In all cases, the mice treated with KM-110 showed more effective anti-tumor metastatic activity than the mice of Helixor. These results suggest that Korean mistletoe extracts, KM-110 might be used as an alternative methods having antitumor activity like European mistletoe preparation, Helixor.

• 한국식품영양과학회지
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• 제38권5호
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• pp.529-534
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• 2009

추출용매 및 방법을 달리하여 추출한 겨우살이의 생리활성을 측정하였다. 겨우살이는 microwave 방법으로 물, 50% 에탄올, 100% 에탄올의 용매를 이용하여 추출한 뒤 이의 생리활성을 측정하였다. 총 폴리페놀 함량 측정 시 원재료(slice) 추출물에 비해 분말상태(powder) 추출물의 polyphenol 함량이 높았다. 특히 50% 에탄올 추출구에서 물과 100% 에탄올 추출구보다 높은 함량을 보였다. 전자공여 작용 역시 분말(powder) 상태의 겨우살이 추출물에서 그 활성이 높게 나타났으며, 그 활성은 90% 이상으로 기준물질인 L-ascorbic acid보다 높은 결과를 보였다. 아질산염은 전자공여능과 총 폴리페놀함량과 마찬가지로 원재료(slice)보다분말(powder) 추출구에서 더 높은 소거능을 보였으며, pH의 증가에 따라 점점 감소하는 경향을 보였다. 반면 SOD는 40% 이하의 낮은 활성을 보였으며, tyrosinase 역시 20% 이하로 낮은 결과를 보였다. 결론적으로 겨우살이 추출물은 SOD 유사활성 및 tyrosinase 저해효과는 높지 않지만 우수한 전자공여능과 아질산염 소거능이 있는 것으로 판단되므로 추출 시 시료의 양이나 추출물의 농도를 조금씩 높여준다면 천연 항산화제로서 이용 가능성을 높일 수 있을 것으로 판단되었다.

• Journal of Animal Science and Technology
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• 제64권5호
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• pp.970-984
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• 2022

Thermal stress is a tremendous health predicament encountered by poultry farmers with adverse effects on the performance, product stature, health condition, survival, and overall welfare of poultry birds, and so requires urgent dietary user-friendly strategy to curb. This study was conducted with 200-day old broilers for the purpose of investigating the potential of phytogenics in refining the negative effects of heat stress on broiler chicken. Moringa, Phyllanthus and mistletoe leaves were processed as phytogenic supplements and incorporated into standard ration for broilers as treatments B1 (control), B2, B3 and B4 diet during the peak of thermal discomfort in humid tropics. Growth and carcass indices were monitored in a 49-day trial and blood samples were harvested at the end of the ordeal period to assess haematology, serum biochemical and oxidative stress markers with the use of standard procedures. The results obtained showed that the prevailing environmental condition in the study site indicated that the birds were exposed to heat stress. Birds fed on moringa and mistletoe supplements had higher performance index than birds without supplementation during heat stress condition, while birds fed on mistletoe supplement had the highest survival rate across the treatments. The liveweight, slaughter weight, dressed weight and eviscerated weight of heat stressed birds fed on moringa, phyllanthus and mistletoe supplements were significantly higher than birds on control treatment. Heterophyl/lymphocyte ratio of heat stressed birds without supplement were higher than birds on phytogenic supplements, with least values recorded in phyllanthus and mistletoe fed birds. Birds on phytogenic supplement tend to have lower cholesterol profile, lipid peroxidation and better antioxidant profile than birds on control treatment during heat stress conditions. Mistletoe supplementation in broiler ration enhances the survival rate, as well as promotes growth indices better among the phytogenic supplements. However, phytogenic supplements did ameliorate the negative effects of thermal discomfort on performance, physiological and oxidative stress in heat-stressed broiler chicken.