YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Isolation and Characterization of Lectin from Viscum coloratum

겨우사리 중의 렉틴성분 분리 및 특성

  • Park, Won-Bong (Department of Chemistry, College of Natural Sciences, Seoul Woman's University) ;
  • Kim, Hee-Sook (Department of Chemistry, College of Natural Sciences, Seoul Woman's University)
  • 박원봉 (서울여자대학교 자연과학대학 화학과) ;
  • 김희숙 (서울여자대학교 자연과학대학 화학과)
  • Published : 1994.08.30
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Abstract

Lectin from mistletoe (Viscum coloratum) were obtained by salt fractionation, gel filtration and anion exchange chromatography. A molecular weight of about 60,000D has been determined by SDS-PAGE and two basic subunits which have molecular weights of 33,000D and 28,000D are linked by a disulfide bond. The partially purified mistletoe lectins agglutinated human B erythrocytes. Agglutinating activity was relatively stable at varied pH $(3.77{\sim}8.71)$ and at temperature range of $0{\sim}40^{\circ}C$ and not affected by 9 metal ions. Galactose, lactose and N-acetyl-D- galactosamine inhibited agglutinating activity of lectin. Lectin from mistletoe was more mitogenic to murine lymphocytes than concanavalin A.

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