• Asian-Australasian Journal of Animal Sciences
• /
• v.33 no.2
• /
• pp.360-372
• /
• 2020

Objective: Specific genomic sites can be recognized and permanently modified by genome editing. The discovery of endonucleases has advanced genome editing in pigs, attenuating xenograft rejection and cross-species disease transmission. However, off-target mutagenesis caused by these nucleases is a major barrier to putative clinical applications. Furthermore, off-target mutagenesis by genome editing has not yet been addressed in pigs. Methods: Here, we generated genetically inheritable α-1,3-galactosyltransferase (GGTA1) knockout Yucatan miniature pigs by combining transcription activator-like effector nuclease (TALEN) and nuclear transfer. For precise estimation of genomic mutations induced by TALEN in GGTA1 knockout pigs, we obtained the whole-genome sequence of the donor cells for use as an internal control genome. Results: In-depth whole-genome sequencing analysis demonstrated that TALEN-mediated GGTA1 knockout pigs had a comparable mutation rate to homologous recombination-treated pigs and wild-type strain controls. RNA sequencing analysis associated with genomic mutations revealed that TALEN-induced off-target mutations had no discernable effect on RNA transcript abundance. Conclusion: Therefore, TALEN appears to be a precise and safe tool for generating genomeedited pigs, and the TALEN-mediated GGTA1 knockout Yucatan miniature pigs produced in this study can serve as a safe and effective organ and tissue resource for clinical applications.

• The Transactions of the Korean Institute of Power Electronics
• /
• v.14 no.2
• /
• pp.112-119
• /
• 2009

This paper presents a PLL(Phase Lock Loop) approach for effective speed and torque control of high speed miniature BLDCM(Brushless DC Motor) using hall sensor. The proposed speed control method based on PLL uses only a phase shift between reference pulse signal according to speed reference and actual pulse signal from hall sensor. It doesn't use any speed calculation, and calculates a direct current reference from phase shift. The current reference is changed to reduce the phase shift between reference and actual pulse. So the actual speed can keep the reference speed. The proposed control scheme is very simple but effective speed control is possible. In order to obtain a smooth torque production, the reference current is changed using acceleration and deceleration slope. The proposed control scheme is verified by experimental results of the 50W, 40,000[rpm] high speed miniature BLDCM.

• Transactions of the Korean Society of Mechanical Engineers B
• /
• v.26 no.3
• /
• pp.439-447
• /
• 2002

The microchannel flow in miniature TCDs (thermal conductivity detectors) is investigated numerically. The solutions based on the boundary layer approximation are not very accurate in the region of the duct inlet for low Reynolds numbers. In this study, two-dimensional Navier-Stokes equations are considered to analyze the gas flow in a miniature TCD. Effects of channel size, inlet and boundary conditions on the heat transfer rate are examined. When the gas stream is not preheated, the distances for a miniature TCD to reach the conduction-dominant region for duct flow are found to be approximately two and three times the thermal entry length for duct flow with constant properties, respectively, leer constant wall temperature and constant wall heat flux boundary conditions. If the gas temperature at the channel inlet is close to the mean gas temperature in the conduction-dominant region, the entrance region is much shorter compared to other cases considered in this study.

• Transactions of Materials Processing
• /
• v.19 no.5
• /
• pp.283-289
• /
• 2010

Extrusion process in the bulk material for fabrication of miniature helical gears has problems such as a high forming load and short tool life because the cross-section is complex and asymmetry. To overcome these problems, in this study, miniature helical gears were fabricated by Zn-22Al powder hot extrusion. The included die angle for minimum extrusion load and improving die filling was determined by FE-simulation. The Zn-22Al spheroidal powder produced by gasatomization were compacted and sintered for extrusion experiment. The dimension of helical-gear is 0.3mm in module, 3.35mm in pitch diameter, $15^{\circ}$ in helix angle and the number of teeth is 12. All of the extrusion experiments were performed with internal helical gear die which was machined by precision electric discharge machining using the electrode. The experiment was conducted at $190^{\circ}C$ to $310^{\circ}C$ to obtain extrusive and mechanical properties. The extruded helical gears were analyzed through extrusion load, Vickers hardness and SEM images for each extrusion temperature. The powder hot extrusion process was successfully applied to fabricate a miniature helical gear.

• Journal of Animal Science and Technology
• /
• v.62 no.3
• /
• pp.348-355
• /
• 2020

Cyclophosphamide, a cytotoxic anticancer agent, induces immunosuppression and has several adverse effects. N-acetylcysteine alleviates oxidative stress, liver injury, and intestinal tissue damage. The present study examined whether N-acetylcysteine modulates the adverse effects of cyclophosphamide in pigs. Miniature pigs (n = 15) were used as an experimental model to evaluate the effects of N-acetylcysteine treatment on immune reactions, liver injury, and oxidative stress after cyclophosphamide challenge. Corn-soybean meal based dietary treatments were as follows: control diet with either saline injection, cyclophosphamide injection, or 0.5% N-acetylcysteine and cyclophosphamide injection. N-acetylcysteine increased the number of immune cells and decreased TNF-α production after cyclophosphamide injection and decreased TNF-α, IFN-γ, NF-κB, and IL-8 expression and increased IL-10 expression in peripheral blood mononuclear cells. Serum levels of alanine transaminase and aspartate aminotransferase decreased, superoxide dismutase activity increased, and malondialdehyde activity decreased following N-acetylcysteine treatment after cyclophosphamide injection. N-acetylcysteine decreases immunosuppression, liver injury, and oxidative stress in cyclophosphamide-challenged miniature pigs. The present study suggests that N-acetylcysteine has therapeutic application in livestock for modulating immune reactions, liver injury, and oxidative stress.

• Journal of Advanced Marine Engineering and Technology
• /
• v.31 no.4
• /
• pp.343-349
• /
• 2007

This cryogenic miniature globe valve is used to transfer the liquified natural gas which temperature is $-169^{\circ}C$, supplied pressure is 30bar(3.0MPa). In the present work the temperature distribution and thermal deformation is calculated numerical the FE method is useful to predict the thermal matter of cryogenic miniature globe valve. For this reason, to optimum design of the cryogenic miniature globe valve the analysis of the parameter about bonnet has been studied. It's used 3-D modeling to analyze cryogenic globe valve, which is 1/2". Numerical study used 3-D modeling makes a decision of efficient process of product before producing in the factory. A commercial software(ANSYS 10.0) is used in the structural analysis for cryogenic globe valve.

• Acta Via Serica
• /
• v.3 no.1
• /
• pp.119-138
• /
• 2018

The dragon of the East was an object of worship and an authority to make rain, unlike the West. The dragon image, one of the positively accepted Chinese motifs with the blue-and-white porcelain of the Ming dynasty by the Ottoman Empire in the 16th century, was combined with gigantic saw-edged leaves to create a genre in Saz style. By combining Eastern dragons with plant motifs instead of clouds, dragons were no longer accepted as authority and nobility but as symbols of life and longevity. Unlike Iran and other countries, the image of dragons in Turkish miniature paintings has evolved into a unique style using Turkish calligraphy. The stylistic feature is that a thick black line that gives the impression of calligraphy forms the dragon's back or a huge saz leaf stalk and forms the axis of the screen. Most of the work was black ink drawing, not painting, and partly lightly painted. In the development stage, the dragon appears as a protagonist on the screen of the early works, but the dragon retreats to the latter half and the saz leaves play a leading role on the screen. A common feature in all paintings, whether early or late, is that they have a militant character and create tension on the screen. From the viewpoint of comparative culture, Turkish dragon miniature drawings of the 16thcentury Ottoman period and the Joseon dynasty are somewhat similar in that they are based on calligraphic character and desire for longevity and loyalty, and are drawn according to certain iconic principles.

• Journal of Veterinary Clinics
• /
• v.30 no.2
• /
• pp.111-114
• /
• 2013

A 4-year-old spayed female Miniature Pinscher was presented with a 3.5-year history of elevated liver enzymes level. Continuous elevation of liver enzymes was found on repeated blood examinations and the dog was referred for further evaluation. The abdominal ultrasonography revealed that the gallbladder could not be visualized in any plane. The absence of gallbladder was suspected on ultrasonography. Computed tomography (CT) confirmed gallbladder agenesis (GBA). This is the first reported case of diagnosis of GBA in Miniature Pinscher by using CT.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
• /
• v.32 no.4
• /
• pp.327-332
• /
• 2019

Recently, inverter control systems have attracted immense attention to increase the energy efficiency. However, such systems use repeated on/off high currents for linear operation control, instead of the prevalent step variable current control method. Hence, there arise concerns of personal and property damage, especially due to the durability, explosive characteristics, and operating speed of the fuse, which is responsible for safety and is one of the internal components using current control. Therefore, in this paper, we propose an IEC60127-4 SMD sub-miniature fuse, consisting of Ag-Cu alloys and ceramic powder for arc soothing. The IEC60127-4 SMD sub-miniature fuse has high durability and cut-off capacity, and operates safely in dangerous circumstances caused by the inverter control system.

• Animal cells and systems
• /
• v.15 no.4
• /
• pp.310-314
• /
• 2011

The Banna miniature pig (BNMP) is a representative miniature pig breed in China. Even though BNMP dwarfism is obvious, its underlying causative mutations remain unknown. In this study, the BNMP and Large White pig (LWP) serum growth hormone (GH) and insulin-like growth factor (IGF-1) levels were detected by ELISA and compared. BNMP serum IGF-1 levels were significantly lower than LWP levels (P<0.05). The miniature condition may arise from mutations in the GH and GH receptor (GHR) genes. Therefore, GH and GHR cDNA from the BNMP were cloned into a pMD18-T vector by RT-PCR using the total RNA obtained from the BNMP's pituitary and liver tissues. Sequencing results indicated that the open reading frame of the BNMP GH gene is composed of a 26-residue signal peptide and a 191-residue mature peptide. The coding sequence of the BNMP GHR gene contained 639 amino acids, including a signal peptide that is 18 amino acids long. Two amino acid substitutions, A09V and R22Q, were found in the signal peptide of the GH gene. Additionally, the S104P mutation was found in the BNMP's mature GH protein. Four mutations in the cytoplasmic domain of GHR may influence the downstream signal transduction of GHR, which needs further experimental evidence.