• Korean Journal of Microbiology
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• v.47 no.1
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• pp.30-37
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• 2011

This ultra real-time PCR (UPCR) based diagnosis system for avian influenza A virus (AIV) subtype was designed. The target primer in this study was derived from H5N1 subtype-specific 133 bp partial gene of hemagglutinin (HA), and was synthesized by using PCR-based gene synthesis on the ground of safety. UPCR was operated by Mini-Opticon Q-PCR Quantitative Thermal Cycler using aptamer-based molecular beacon, total 10 ${\mu}l$ of reaction mixture with extraordinarily short time in each steps in PCR. The detection including UPCR and analysis of melting temperature was totally operated within 15 min. The AIV-specific 133 bp PCR product was correctly amplified until 5 molecules of HA gene as minimum of templates. This kind of PCR was drafted as UPCR in this study and it could be used to detect not only AIV subtype, but also other pathogens using UPCR-based diagnosis.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.29 no.10B
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• pp.849-856
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• 2004

This paper proposes a novel superframe formation algorithm for wireless systems with time-slotted superframe structure. The design objective of the proposed algorithm is to provide high performance, stability, error tolerance, as well as power-saving features. The paper presents a comprehensive performance analysis of the Proposed superframe formation algorithm with different types of traffic applied. During analysis several performance metrics are investigated. The error tolerance to beacon loss and the behavior of CBR flows are also studied. We show that the proposed algorithm inherits the advantages of the previously used algorithms, while providing additional features.